Optimization ntawm Extraction txheej txheem ntawm Polysaccharide Los ntawm Cistanche Deserticola Los ntawm Teb Txheej Txheej Txheej Txheej

Lub Hom Phiaj:Txhawm rau txiav txim siab qhov zoo tshaj plaws rau kev rho tawm polysaccharides los ntawm Cistanche deserticola los ntawm kev rho tawm dej kub. Txoj kev: Txoj kev rho tawm dej kub tau raug xaiv los rho tawm polysaccharides los ntawm Cistanche deserticola deserticola, Cistanche deserticola deserticola tau siv los ua cov khoom siv raw, thiab tus nqi rho tawm ntawm polysaccharide los ntawm Cistanche deserticola deserticola tau siv los ua tus nqi teb. Qhov zoo tshaj plaws ntawm ib qho kev mob tshwm sim tau pom los ntawm kev sim ib leeg, thiab cov txiaj ntsig kev sim ib leeg tau ua kom zoo los ntawm cov lus teb saum npoo txheej txheem. Qhov tshwm sim: Cov txiaj ntsig kev sim qhia tau hais tias qhov zoo tshaj plaws rau cov dej kub rho tawm txoj kev yog: kua rau cov khoom sib piv ntawm 45: 1 (mL: g), dej kub extraction kub ntawm 100 degree, lub sij hawm ntawm 130 feeb, thiab ethanol los nag concentration ntawm 100 feem pua. Raws li cov xwm txheej no, tus nqi rho tawm polysaccharide ntawm Cistanche deserticola yog 11.207 feem pua, uas yog ze heev rau qhov kwv yees tus nqi ntawm 11.609 feem pua ​​​​los ntawm cov lus teb saum npoo txheej txheem. Xaus: Qhov kev sim ua pov thawj tias qhov kev ua kom zoo ntawm polysaccharide extraction ntawm Cistanche deserticola los ntawm cov lus teb saum npoo yog tsim nyog thiab txhim khu kev qha, uas tuaj yeem muab lub hauv paus txhim khu kev qha rau cov kauj ruam tom ntej ntawm kev tsim khoom.

Ntsiab lus: Cistanche deserticola; Txoj kev rho tawm dej kub; Polysaccharides; Tus nqi rho tawm; Teb nto txoj kev; Ua kua rau cov khoom sib piv; Kub; Lub sij hawm; Dej cawv concentration

Cov tshuaj tseem ceeb ntawm Cistanche deserticola

Cistanche deserticola deserticola hauv Inner Mongolia yog ib tsob nroj uas muaj hnub nyoog, uas feem ntau loj hlob hauv huab cua thiab qhuav qhuav, thiab muaj txiaj ntsig zoo tshuaj. Cistanche deserticola nyiam lub koob npe nrov ntawm suab puam ginseng vim nws tshwm sim feem ntau nyob rau hauv keeb kwm ntawm Suav tshuaj ntsuab tshuaj rau tonics. Cistanche deserticola deserticola hauv Inner Mongolia muaj ntau yam tshuaj lom neeg, feem ntau suav nrog Phenethyl cawv glycosides, polysaccharides, flavonoids, thiab lwm yam. Tsis tas li ntawd, muaj cov amino acids, inorganic kab kawm, thiab lwm yam. Ib qho ntawm nws lub ntsiab Cheebtsam, polysaccharides, muaj ib tug immunomodulatory. Cov nyhuv, tuaj yeem pab lymphocyte proliferation, txhim kho kev tiv thaiv kab mob hauv lub cev, thiab ua kom cov macrophages, nrog rau kev tswj hwm ntawm kev tiv thaiv kab mob. Cistanche deserticola deserticola polysaccharide kuj muaj cov teebmeem ntawm kev tiv thaiv oxidation, tiv thaiv kev laus, txo kev qaug zog, soothing cov hnyuv thiab catharsis, txhim kho cov tsos mob ntawm Parkinson's disease, txhim kho kev kawm thiab kev nco, tiv thaiv cov hlab ntsha, tiv thaiv kab mob, thiab tiv thaiv qog. Tam sim no, hauv Alxa, Inner Mongolia, koob Cistanche deserticola tau cog thiab ua tiav. Kev lag luam saw ntawm kev muag khoom tau raug txhim kho. Nws yog nyiaj ntsuab qoob loo zoo thiab tau dhau los ua kev lag luam hauv zos. Ua ke nrog kev tswj hwm kev lag luam desertification, nws tau txo qis hauv cheeb tsam Desertification ib puag ncig thiab muaj kev cia siab rau txoj kev loj hlob. Ntawm lawv, cov tshuaj siv tshuaj ntawm polysaccharides hauv Cistanche deserticola deserticola tau dhau los ua qhov kev tshawb fawb hotspot, thiab yuav ua li cas txhawm rau txhim kho tus nqi rho tawm ntawm Cistanche deserticola deserticola polysaccharides hauv Inner Mongolia tau dhau los ua qhov txuas tseem ceeb. Qhov kev sim no tau xaiv txoj kev rho tawm dej kub los rho cov polysaccharides. Tam sim no, txoj hauv kev rau extracting polysaccharides muaj xws li microwave-pab extraction, ultrasonic-pab extraction, thiab enzymatic-pab extraction. Txoj kev siv microwave-pab extraction tuaj yeem ua rau cov cua sov tsis sib xws ntawm cov khoom siv tshuaj, ua rau cov khoom siv deactivation. Txoj kev pabcuam ultrasonic yog qhov tsim nyog tshaj plaws rau kev rho tawm polysaccharides los ntawm cov nroj tsuag uas muaj cov dej noo siab, thaum peb lub chaw kuaj mob siv cov khoom siv tshuaj qhuav qhuav, uas tsis haum. Enzymatic extraction yuav tsum muaj kev sim siab thiab kev ua haujlwm nyuaj, yog li qhov yooj yim tshaj plaws thiab ruaj khov dej extraction txoj kev raug xaiv rau cov kauj ruam tom ntej ntawm kev tshawb fawb. Tom qab ntawd, cov txheej txheem teb saum npoo yog siv los tshawb nrhiav qhov zoo tshaj plaws extraction tej yam kev mob rau Cistanche deserticola polysaccharide extraction, muab kev pab txhawb rau industrialization ntawm Cistanche deserticola polysaccharide ntau lawm hauv Inner Mongolia. Nws muab kev txhawb nqa theoretical rau kev kawm ntxiv ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia.

Cov tshuaj tseem ceeb ntawm Cistanche deserticola

Nyem qhov no mus saib Cistanche cov khoom

【Nug ntxiv】 Email: cindy.xue@wecistanche.com / Whats App: 0086 18599088692 / Wechat: 18599088692

1 Cov khoom siv thiab cov txheej txheem

1.1 Xeem tshuaj thiab tshuaj reagents

Cistanche deserticola deserticola (Inner Mongolia Hongkui Cistanche Group Co., Ltd., ntau lawm batch naj npawb: PDD 236305955677); D anhydrous qabzib tus qauv (China Foods Limited thiab Drug Identification Institute, batch tooj: 11508 201602); Ethanol (Xilong Chemical, ntau lawm batch tooj U1000510 500mL), concentrated sulfuric acid (sigma, ntau lawm batch tooj 7664 93 9 nyob rau hauv lub tebchaws United States), thiab phenol (sigma, ntau lawm batch tooj 108 95 2 nyob rau hauv Tebchaws Meskas) feem ntau yog siv cov tshuaj reagents, tag nrho cov tshuaj ntsuam xyuas dawb huv.

1.2 Cov cuab yeej thiab cov cuab yeej siv

Keyili Swing High-Speed ​​Universal Crusher (Zhejiang Yili Kev Lag Luam thiab Kev Lag Luam Co., Ltd., qauv: QE-1000); Hluav taws xob tshuab ziab khaub ncaws (Shanghai Yiheng Scientific Instrument Co., Ltd., qauv: DHG 9240A); Xinyue pom spectrophotometer (Beijing Puxi General Instrument Co., Ltd., qauv: T6); Multi tube tsis siv neeg tshuav nyiaj li cas centrifuge (Shanghai Luxiangyi Centrifuge Instrument Co., Ltd., qauv: TD5M); Peb lub hom phiaj qhov dej kub tsis tu ncua (Tianjin Taist Instrument Co., Ltd., qauv: SHHW21-600A Hom II); Electronic tshuav nyiaj li cas [Auhaus Instruments (Changzhou) Co., Ltd., qauv: PX224ZH].

Cistanche deserticola kev sim

2 Txoj kev

2.1 Cov dej kub rho tawm thiab rho tawm ntawm polysaccharides

Ua ntej, Cistanche deserticola hauv Inner Mongolia tau qhuav ntawm 65 degree thiab tom qab ntawd crushed nrog lub grinder, tshuaj xyuas nrog 60 mesh sieve, thiab tag nrho cov seem dhau 60 mesh sieve yuav siv tau. Ua kom raug hnyav 10g ntawm Cistanche deserticola hmoov thiab tsau rau hauv dej kub raws li qhov kub thiab lub sijhawm rau kev tshem tawm dej kub. Cov tau txais extract tau centrifuged ntawm qhov ceev ntawm 3000r / min (nrog lub vojvoog ntawm 18cm) rau 10 feeb kom tau txais lub supernatant. 95 feem pua ​​​​ethanol, uas yog peb npaug ntawm qhov ntim ntawm supernatant, tau nchuav rau hauv supernatant thiab sib tov zoo. Tom qab ntawd, nws raug tso cai kom daws tau 12 teev nrog cawv. Lub precipitate tau khaws cia los ntawm centrifugation ntawm qhov ceev ntawm 3000r / min (nrog lub vojvoog ntawm 18cm) rau 2 feeb, thiab ntxuav nrog dej tsis muaj ethanol. Thaum ethanol volatilizes lawm, nws tsim cov crude polysaccharide ntawm Cistanche deserticola hauv Inner Mongolia.

2.2 Kev tsim cov qauv nkhaus

Pipette 0 1mg / mL ntawm cov qauv piam thaj tov thiab dilute nws cais mus rau 10 μ G / mL, 20 μ G / mL, 30 μ G / mL, 40 μ G / mL, 50 μ G / mL, 60 μ G / mL, 70 μ G / mL, 80 μ G / mL, 90 μ G / mL, siv phenol sulfuric acid txoj kev siv lub spectrophotometer λ Ntsuas absorbance rau 490nm. Qhov kev sib raug zoo ntawm qhov loj ntawm cov piam thaj thiab absorbance yog Y=0.015X{13}}.012, R{15}}.995.

Daim duab 1 Glucose Standard Curve

2. 3 Qhov piv ntawm cov kua rau cov khoom cuam tshuam rau kev rho tawm ntawm polysaccharides los ntawm Cistanche deserticola hauv Inner Mongolia

Cov polysaccharide ntawm Cistanche deserticola tau muab rho tawm los ntawm 95 feem pua ​​​​ethanol nag lossis daus ntawm 90 degree, 120 min, thiab 90 degree hauv dej kub. Qhov piv ntawm cov kua rau cov khoom yog teem rau ntawm 10 ∶ 1, 20 ∶ 1, 30 ∶ 1, 40 ∶ 1, 50 ∶ 1, thiab 60 ∶ 1 (mL ∶ g).

2.4 Lub sij hawm rho tawm dej kub cuam tshuam qhov kev rho tawm ntawm polysaccharides los ntawm Cistanche deserticola hauv Inner Mongolia

Cov polysaccharide ntawm Cistanche deserticola tau muab rho tawm nrog qhov sib piv ntawm cov kua rau cov khoom siv ntawm 40 ∶ 1 (mL ∶ g), qhov kub ntawm cov extraction ntawm 90 degree, thiab 95 feem pua ​​​​ethanol raws li qhov kev sim kho. Lub sijhawm tshem tawm cov dej kub tau siv los ua qhov sib txawv, thiab cov kev sim tau ua nrog 30 min, 60 min, 90 min, 120 min, 150 min, thiab 180 min, raws li. Cov ntsiab lus polysaccharide tau txiav txim siab thiab tus nqi rho tawm raug xam.

2. 5 Cov nyhuv ntawm cov dej kub extraction kub ntawm qhov extraction tus nqi ntawm polysaccharide los ntawm Cistanche deserticola

Cov polysaccharide ntawm Cistanche deserticola tau muab rho tawm raws li cov xwm txheej ntawm cov khoom siv ua kua sib piv ntawm 40 ∶ 1 (mL ∶ g), lub sijhawm rho tawm ntawm 150 min, thiab 95 feem pua ​​​​ethanol nag lossis daus. Cov dej kub rho tawm kub tau teeb tsa raws li qhov sib txawv, thiab qhov kev sim tau ua ntawm 60 degree, 70 degree, 80 degree, 90 degree, thiab 100 degree. Cov ntsiab lus polysaccharide tau txiav txim siab thiab tus nqi rho tawm polysaccharide raug xam.

2 .6 Ethanol concentration cuam tshuam tus nqi rho tawm ntawm polysaccharides los ntawm Cistanche deserticola hauv Inner Mongolia

Kev rho tawm ntawm polysaccharides los ntawm Inner Mongolia Astragalus membranaceus yog ua los ntawm cov kua-rau-cov ntaub ntawv piv ntawm 40: 1 (mL: g), lub sij hawm rho tawm dej kub ntawm 150min, thiab dej kub extraction kub ntawm 100 degree. Lub ethanol nag lossis daus concentration tau teem rau ntawm 60 feem pua, 70 feem pua, 80 feem pua, 85 feem pua, 90 feem pua, 95 feem pua, thiab 100 feem pua ​​rau kev sim. Cov ntsiab lus polysaccharide tau ntsuas thiab ntsuas tus nqi rho tawm.

2.7 Response Surface Optimization Design

Tom qab ua tiav qhov kev sim saum toj no ib leeg xwb, cov txiaj ntsig tau los ntawm qhov kev sim ib leeg-tseem ceeb saum toj no yuav raug txiav txim siab raws li qib siab, thiab cov lus teb qhov chaw optimization tsim yuav ua tiav. Cov kev teeb tsa tshwj xeeb muaj nyob hauv Table 1.

Table 1 Ib theem teeb tsa ntawm Cistanche deserticola polysaccharide extraction txheej txheem hauv Inner Mongolia

Plaub yam thiab peb theem Tsim kev sim yog ua los ntawm kev siv Design Exper11 software raws li Box Benhnken tsim qauv. Cov phiaj xwm sim thiab cov ntsiab lus teb saum npoo yog qhia hauv Table 2.

Table 2 Teb Cov Qauv Tsim Nto thiab Cov Nqis Teb

Siv Design Exper11 software los ua ntau yam regression haum ntawm cov ntaub ntawv kab hauv Table 1 tau los ntawm qhov kev sim no, Thaum kawg, tus nqi rho tawm (Y) ntawm polysaccharide los ntawm Cistanche deserticola hauv Inner Mongolia tau txais quadratic ntau regression simulation kab zauv Y{{2} } plus 9. }.337D plus 0.232AB-0.046AC plus 0.079AD-0 283BC-0 096BD-0 046CD plus 0.076A2-0 282B2-0 130C2 plus 0.190 D2, R2=0.943. Saib Table 3. Tus tsim exper11 software tau siv los txheeb xyuas thiab sib piv cov teebmeem ntawm ob qho xwm txheej ntawm kev rho tawm tus nqi ntawm polysaccharides los ntawm Cistanche deserticola hauv Inner Mongolia, nrog rau qhov piv ntawm A kua rau cov khoom, qhov kub ntawm B dej kub extraction, qhov concentration ntawm C ethanol, thiab lub sij hawm ntawm D dej kub extraction raws li tseem ceeb influencing yam. Saib cov duab 6-11.

3 Kev tshwm sim

3.1 Qhov piv ntawm cov kua rau cov khoom cuam tshuam rau kev rho tawm ntawm polysaccharides los ntawm Cistanche deserticola hauv Inner Mongolia

Nyob rau tib lub sijhawm ntawm kev rho tawm qhov kub thiab txias, lub sijhawm rho tawm, thiab dej cawv nag lossis daus concentration, qhov rho tawm ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia tau pom qhov nce siab ntawm cov kua-rau-cov khoom sib piv ntawm 10 ∶ 1 txog 40 ∶ 1 thiab mus txog qhov siab tshaj plaws. tus nqi ntawm 40 ∶ 1. Tom qab ntawd, qhov kev rho tawm ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia txo qis nrog qhov nce ntxiv ntawm cov kua rau cov khoom siv. Yog li, cov kua-rau-cov ntaub ntawv piv yog 40: 1 (mL: g), uas yog qhov zoo tshaj plaws tus nqi xaiv rau qhov kev sim no. Saib daim duab 2.

Fig. 2 Cov txiaj ntsig ntawm cov khoom siv ua kua sib piv ntawm kev rho tawm tus nqi ntawm polysaccharide los ntawm Cistanche deserticola hauv Inner Mongolia

3. 2 Cov nyhuv ntawm cov dej kub lub sij hawm rho tawm ntawm tus nqi rho tawm ntawm polysaccharide los ntawm Cistanche deserticola

Raws li cov xwm txheej ntawm cov khoom siv ua kua, qhov kub thiab txias, thiab 95 feem pua ​​​​ethanol nag lossis daus, tus nqi rho tawm ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia tau pom qhov kev nce siab hauv cov dej da dej lub sij hawm ntawm 30 ~ 180 min thiab mus txog qhov siab tshaj plaws tus nqi thaum lub Lub sij hawm rho tawm dej kub yog 150 min. Tus nqi rho tawm maj mam ruaj khov thiab tsis nce ntxiv thaum lub sij hawm tshem tawm cov dej kub tau txuas ntxiv. Yog li ntawd, qhov kev sim no tau xaiv qhov zoo tshaj plaws extraction lub sij hawm ntawm 150 feeb. Saib daim duab 3.

Fig. 3 Cov nyhuv ntawm cov dej kub lub sij hawm rho tawm ntawm tus nqi rho tawm ntawm polysaccharide los ntawm Cistanche deserticola hauv Inner Mongolia

3. 3 Cov nyhuv ntawm cov dej kub rho tawm kub ntawm qhov rho tawm ntawm polysaccharide los ntawm Cistanche deserticola

Nyob rau ntawm qhov chaw ntawm tib cov khoom siv ua kua sib piv, lub sij hawm rho tawm, thiab dej cawv nag lossis daus concentration, qhov kev rho tawm ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia tau zoo sib xws nrog qhov kub thiab txias thaum cov dej kub extraction kub yog 60 ~ 100 degree thiab mus txog qhov siab tshaj plaws ntawm tus nqi. 100 degree. Yog li, 100 degree raug xaiv los ua qhov zoo tshaj plaws extraction kub rau qhov kev sim no. Saib daim duab 4.

Fig. 4 Cov nyhuv ntawm cov dej kub rho tawm kub ntawm qhov rho tawm ntawm polysaccharide los ntawm Cistanche deserticola hauv Inner Mongolia

3.4 Ethanol concentration cuam tshuam tus nqi rho tawm ntawm polysaccharide los ntawm Cistanche deserticola hauv Inner Mongolia

Nyob rau tib lub sijhawm ntawm cov khoom siv ua kua sib piv, lub sijhawm rho tawm, thiab qhov ntsuas kub, qhov kev rho tawm ntawm polysaccharide pom qhov nce siab thaum cov ethanol concentration nyob nruab nrab ntawm 60 feem pua ​​​​thiab 95 feem pua, thiab mus txog qhov siab tshaj plaws thaum lub concentration ntawm 1669 · Ntiaj teb tsoos. Cov tshuaj suav suav yog 95 feem pua ​​​​hauv Lub Rau Hli 2023, Volume 18, Qhov Teeb Meem 12. Tom qab ntawd, nrog kev nce ntawm ethanol concentration, qhov rho tawm ntawm crude polysaccharide los ntawm Cistanche deserticola deserticola txo. Yog li, qhov concentration ntawm 95 feem pua ​​​​ethanol yog qhov zoo tshaj plaws rau qhov kev sim no. Saib daim duab 5.

Fig. 5 Cov nyhuv ntawm ethanol concentration ntawm kev rho tawm tus nqi ntawm polysaccharide los ntawm Cistanche deserticola hauv Inner Mongolia

3.5 Box Behnken Test Results and Analysis

Cov txiaj ntsig ntawm kev txheeb xyuas qhov sib txawv yog qhia hauv Table 3.

Table 3 Variance tsom xam cov txiaj ntsig ntawm kev sib npaug regression ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia

Qhov loj ntawm txoj kab nqes ntawm qhov chaw teb, qhov ntau rhiab qhov kev teb tus nqi yog qhov hloov pauv ntawm cov xwm txheej cuam tshuam. Raws li txoj kab nqes loj ntawm daim duab 6-11, qhov loj tshaj plaws uas cuam tshuam rau kev rho tawm ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia yog qhov kub ntawm qhov extraction, uas yog raws li cov txiaj ntsig ntawm ANOVA.

Daim duab 6 Teb rau kev sib cuam tshuam ntawm cov kua rau cov khoom sib piv thiab cov dej kub Extraction kub

Daim duab 7 Teb rau kev sib cuam tshuam ntawm cov kua rau cov khoom sib piv thiab cov dej kub rho lub sij hawm

Daim duab 8 Teb rau kev sib cuam tshuam ntawm Cov Khoom Ua Kua Dej thiab Ethanol Concentration

Daim duab 9 Teb rau kev sib cuam tshuam ntawm Cov Dej Kub Kub Extraction Kub thiab Kub Dej Extraction Sijhawm

Daim duab 10 Teb daim phiaj saum npoo ntawm kev sib cuam tshuam ntawm cov dej kub rho tawm kub thiab ethanol concentration

Xa mus rau qhov zoo tshaj plaws haum extraction tej yam kev mob thiab coj mus rau hauv tus account qhov tseeb qhov teeb meem nyob rau hauv peb lub chaw soj nstuam, qhov kawg optimized extraction txoj kev npaj tau txiav txim raws li nram no: kua-khoom ratio 45:1 (mL: g), extraction kub 100 degree, ethanol concentration 100 feem pua. , thiab lub sijhawm rho tawm 100 feeb. Tus nqi rho tawm ntawm polysaccharides los ntawm Cistanche deserticola hauv Inner Mongolia yog 11.207 feem pua, uas yog ze heev rau qhov kwv yees tus nqi ntawm 11.609 feem pua.

Daim duab 11 Teb rau kev sib cuam tshuam ntawm cov dej kub rho tawm lub sij hawm thiab ethanol concentration

4 Kev sib tham

Hauv kev sim, nws tau pom tias polysaccharide tawm los ntawm qis mus rau siab thiab tom qab ntawd txo qis dua ntawm cov kua-rau-cov khoom sib piv ntawm 20: 1, 30: 1, 40: 1, 50: 1, thiab 60: 1 ( saib daim duab 2). Txawm li cas los xij, tag nrho cov kev hloov pauv tsis yog siab, nrog cov kua-rau-cov khoom sib piv ntawm 20: 1 txog 60: 1. Cov txiaj ntsig ntawm polysaccharides tsuas yog nce li 5 feem pua, thaum cov dej siv tau nce los ntawm ob zaug. Rau kev tsim khoom lag luam, kev npaj loj ntawm polysaccharides ua rau cov dej pov tseg. Nws raug nquahu kom xaiv cov kua-rau-cov khoom sib piv ntawm 20: 1 (mL: g), qhov ntsuas kub ntawm 100 degree, ethanol concentration ntawm 100 feem pua, thiab lub sijhawm rho tawm ntawm 100 feeb. Hauv kev xaus, cov txheej txheem tsis zoo ntawm Cistanche deserticola polysaccharide hauv Inner Mongolia tau txais los ntawm cov txheej txheem teb saum npoo, uas ua tau zoo, ruaj khov, thiab ua tau. Xiao Dao'an [21] siv anthrone sulfuric acid txoj kev thiab orthogonal thwmsim kom tau ib tug polysaccharide extraction tus nqi ntawm 8.17 feem pua ​​thaum cov khoom siv kua piv yog 1:25, qhov kub thiab txias yog 60 degree, thiab lub sij hawm extraction yog 30 feeb. Yang Wen et al. [11] muab rho tawm polysaccharides los ntawm Cistanche deserticola los ntawm ultrasonic txoj kev ua ke nrog enzymolysis. Siv ib qho tseem ceeb thiab kev ntsuam xyuas orthogonal, tus nqi rho tawm yog 5.14 feem pua. Lianle et al. [22] siv ib qho tseem ceeb thiab kev ntsuam xyuas orthogonal los txiav txim siab qhov zoo tshaj plaws extraction tej yam kev mob ntawm Cistanche deserticola polysaccharide extraction tus nqi ntawm 18.90 feem pua.

Suav tshuaj ntsuab cistanche

Tam sim no, nyob rau hauv cov txheej txheem ntawm extracting polysaccharides los ntawm Cistanche deserticola, tsuas yog ib qho kev ntsuam xyuas thiab cov kev ntsuam xyuas orthogonal yog siv, thiab tsuas yog cov cawv ntawm ib qho tseem ceeb ntawm qhov kev sim tau txiav txim siab. Cov txheej txheem tsis sib xws ntawm Cistanche deserticola polysaccharides los ntawm Inner Mongolia yog txhais los ntawm cov lus teb saum npoo, uas tuaj yeem txiav txim siab txog kev cuam tshuam ntawm ntau yam thiab kev cuam tshuam ntawm ob yam ntawm kev tawm los, zam qhov kev tsim ntawm ib sab orthogonal tom qab kev kho mob ntawm ib qho xwm txheej. kuaj. Thiab piv rau lwm txoj kev rho tawm polysaccharide, qhov kev sim no muaj tus nqi qis dua, ntau dua polysaccharide tawm los, thiab muaj kev nyab xeeb, thiab tsim nyog rau kev npaj loj hauv lub Hoobkas. Qhov tsis zoo ntawm qhov kev sim no yog tias txoj kev phenol sulfuric acid tau siv los txiav txim siab cov ntsiab lus ntawm polysaccharides, uas tsis yog tsuas yog txoj hauv kev ua haujlwm tab sis kuj cuam tshuam los ntawm lwm yam tshuaj, uas ua rau muaj qhov tseeb tsawg. Tom qab ntawd, cov txheej txheem tshiab los txiav txim siab polysaccharides tuaj yeem hloov pauv lossis impurities tuaj yeem raug tshem tawm los txhim kho qhov kev sim. Cistanche deserticola hauv Inner Mongolia tsis yog tsuas yog muaj txiaj ntsig zoo rau thaj chaw suab puam hauv Inner Mongolia tab sis kuj tseem muaj txiaj ntsig zoo thiab kho mob. Txawm li cas los xij, nws tsuas yog txwv rau kev tshawb fawb yooj yim tam sim no. Nws cia siab tias cov kev tshawb fawb tob hauv qab no ntawm Cistanche deserticola polysaccharide yuav ua tiav, thiab ntau daim ntawv thov yuav raug tsim hauv cov tshuaj tshiab, khoom noj qab haus huv, zaub mov, thiab lwm yam.

Cistanche extract hmoov

Cov ntaub ntawv

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[14] Li Wu, Wu Shimin, Li Caihong, et al. Optimization of Solidified Fermented Coconut Milk Process by Response Surface Methodology [J]. Tuam Tshoj Brewing, 2019, 38 (2): 212-216

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[17] Aladan McTeilly, Yang Ting, Yuan Jie, et al. Kawm txog cov txheej txheem rho tawm thiab hauv vitro antioxidant kev ua ntawm polysaccharides los ntawm dregs ntawm Cistanche tubulosa [J]. Chemical and Biological Engineering, 2020, 37 (2): 36 41

[18] He Meng, You Lin, Bao Xiaowei, et al. Cov nyhuv ntawm aqueous extract ntawm Cistanche deserticola ntawm antioxidation nyob rau hauv vitro thiab cuam tshuam ntawm Gut microbiota hauv nas ［ J］. Food Studies and Development, 2020, 41 (23): 44  50

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[22] Li Xu, Gao Mingbo, Yin Xinxin, et al. Optimization ntawm ultrasonic extraction txheej txheem tej yam kev mob ntawm Cistanche deserticola deserticola polysaccharide [J]. Guangzhou Chemical Industry, 2018, 46 (19): 61  62, 77