Mechanism Of Herba Cistanches-Epimedium Herb-Fortune's Drynaria Rhizome hauv Kev Kho Mob Osteoporosis Raws li Network Pharmacology Thiab Molecular Docking Ⅱ

Table 1 Cov khoom xyaw nquag ntawm Drynaria fortunei, Cistanche deserticola thiab Epimedium

Daim duab 1 Lub hom phiaj kev sib tshuam cov noob

Daim duab 3 Tshuaj-cov khoom xyaw-lub hom phiaj-stroke phiaj network

GO enrichment tsom xam

5 KEGG pathway analysis

A:MMP-9-Suchilactone;B:MMP-9-quercetin;C:MMP-9-lu－teolin;D:MMP-9-kaempferol

Table 2 Ligand-receptor khi zog

3 Kev sib tham

OP yog ib qho teeb meem loj ntawm cov pob txha metabolism, tshwj xeeb tshaj yog rau cov poj niam hnub nyoog 60 xyoo. Raws li kev cuam tshuam ntawm cov tshuaj hormones, qhov tshwm sim ntawm qhov tshwm simqhov xwm txheej ntawm OPyog nce ntxiv. Cov yam ntxwv hloov pauv ntawm OP yog qhovtus nqi ntawm cov pob txha synthesislos ntawm osteoblasts tsawg duauas yog osteoclasts. Ntxiv rau, yam xws li txocalcium nqus,vitamin D metabolism cuam tshuam, thiabtxo lub raum ua haujlwmtuaj yeem ua rau OP[2]. Nyob rau tib lub sijhawm, OP tuaj yeem ua rau cov pob txha tawg thiab tawg yooj yim.

Lub hom phiaj tseem ceeb ntawm kev kho OP yog tiv thaiv kev puas tsuaj, thiab ntau yam tshuaj xws libisphosphonates, Denosumab, teriparatide, thiab abaloparatide feem ntau siv rau hauv kev kho mob. Cov txiaj ntsig kho mob ntawm kev kho OP los ntawm kev txhawb nqa cov synthesis ntawm osteoblasts lossis inhibiting qhov kev txiav txim ntawm osteoclasts yog tsis meej heev.

HIGH GLYCOSIDE ECHINACOSIDE CISTANCHE EXTRACT RAU HAUV

pom tseeb, thiab kuj muaj qee yam kev mob tshwm sim. Cov tshuaj suav tshuaj tseem ceeb tshwj xeeb hauv kev kho OP. Suav tshuaj ntsuab xws li Drynariae, Epimedium, thiab Cistanche deserticola yog suav tias yog ntau tus kws kho mob hauv Suav teb ua cov khoom siv tshuaj zoo hauv kev kho mob OP. Lub ntsiab Cheebtsam ntawm Drynariae muaj xws li flavonoids, terpenes, phenolic acids, thiab lwm yam, uas muaj ib tug zoo kho nyhuv ntawm OP [3, 4]. Epimedium, raws li cov nroj tsuag herbaceous ntawm tsev neeg Berberidaceae, feem ntau siv rau hauvtshuaj suav tshuaj noj raum, thiab aphrodisiac, ntxiv dag zog rau cov leeg thiab pob txha, thiab tshem tawm rheumatism nrog rau lwm yam tshuaj. Nyob rau hauv xyoo tas los no, ntau thiab ntau cov kev tshawb fawb tau pom tias epimedium glycosides kuj muaj kev tiv thaiv kab mob thiab tiv thaiv kab mob [5, 6]. Raws li metabolomics

TRADITIONAL CHINESE DAIM NTAWV THOV KOM TAU TXAIS KEV KHO MOB

Cov kev tshawb fawb tau pom tias Epimedium muaj kev kho mob ntawm OP [7]. Cov tshuaj suav tshuaj suav tshuaj suav tshuaj icariin, Anemarrhena saponin thiab ferulic acid tuaj yeem txhawb kev nthuav qhia ntawm pob txha matrix thiab tswj UMR-106 los ntawm BMP thiab Wnt/ -catenin signaling pathways. Osteoblast osteogenesis [8].

Raws li cov nroj tsuag succulent loj hlob nyob rau sab qaum teb kuv lub teb chaws, Cistanche deserticola hlob nyob rau hauv cov suab puam thiab arid cheeb tsam. Nws yog thawj zaug sau tseg hauv Compendium ntawm Materia Medica los kho lub raum yang deficiency, mob thiab tsis muaj zog ntawm lub duav thiab lub hauv caug, thiab hu ua "desert ginseng". Cov khoom tseem ceeb ntawm Cistanche deserticola muaj xws li cistanche deserticola glycoside, eugenol glycoside, -sitosterol, carotene, amino acids thiab polysaccharides. Cov kev tshawb fawb tau pom [9] tias Cistanche deserticola tag nrho glycosides tuaj yeem qhib GSK-3 kom degrade -catenin yam los ntawm Wnt/ -catenin signaling pathway, yog li cuam tshuam rau lub voj voog ntawm tes, txhawb nqa cell apoptosis, txwv kev tsiv teb tsaws ntawm tes, thiab inhibiting siab. mob qog noj ntshav.

Wang F et al. [10] tau qhia tias Cistanche deserticola tag nrho glycosides thiab polysaccharides tuaj yeem txhawb kev tsim cov pob txha osteogenic hauv SAMP6 nas los ntawm kev ua kom Wnt / -catenin signaling pathway, txhim kho pob txha microstructural puas, thiab kho OP. Hauv txoj kev tshawb no stigmasterol, beta-sitosterol, kaempferol,

Muaj tag nrho ntawm 46 cov khoom xyaw tseem ceeb suav nrog luteolin, Icariin, quercetin, chryseriol, thiab lwm yam. Lub hom phiaj tseem ceeb ntawm kev koom tes yog AKT1, ERS2, MMP-9, AR, mTOR, thiab lwm yam. Cov kev tshawb fawb pom tau tias Drynariae, Epimedium, thiab Cistanche deserticola tebchaw tuaj yeem kho OP los ntawm yam xws li AKT, mTOR, thiab lwm yam. Txhawm rau txhawm rau txheeb xyuas ntxiv Drynariae, Epimedium, thiab Cistanche deserticola, Epimedium rau OP metabolism mechanism. Txoj kev tshawb no siv GO enrichment thiab KEGG tsom xam, thiab cov txiaj ntsig tau pom tias cov molecules koom nrog hauv peb cov tshuaj yog ntau lub hom phiaj. GO

Cov kev nkag muaj xws li inflammatory yam tseem ceeb IL-2, mitogen-activated protein kinase MAPK, apoptosis factor CASPASE, thiab lwm yam. Cov lus qhia ntawm IL-2 plays lub luag haujlwm tseem ceeb hauv Wnt / txoj hauv kev ntawm kab mob metabolic pob txha [11 ].

Tib lub sijhawm, kev tshawb fawb tau pom tias puerarin inhibits osteoclastogenesis los ntawm inhibiting TRAF6/ROS-dependent MAPK/NF-κB signaling pathway, li no alleviating osteoporosis in ovariectomized nas [12]. Cov txiaj ntsig saum toj no qhia tau hais tias cov tshuaj suav tshuaj Drynariae, epimedium, thiab Cistanche deserticola tuaj yeem kho OP los ntawm kev tswj kev mob, inhibiting pob txha cell apoptosis, thiab tswj cov metabolism. KEGG cov txiaj ntsig tau pom tias txoj hauv kev koom nrog Drynariae, Epimedium, thiab Cistanche deserticola feem ntau suav nrog txoj hauv kev mob qog noj ntshav, txoj hauv kev qhia cov tshuaj estrogen, PI3K-Akt signaling pathways, thiab lwm txoj hauv kev. Hauv cov kab mob ntsig txog cov pob txha metabolism, PI3K-Akt txoj hauv kev ua lub luag haujlwm tseem ceeb ua lub hub metabolic. Cov kev tshawb fawb tau pom [13] tias kev ua kom lub cev muaj zog thiab cov qe ntshav liab tuaj yeem ua rau osteogenesis thiab angiogenesis ua rau OP tom qab lub cev tsis muaj zog los ntawm kev tswj hwm PI3K-Akt signaling pathway.

TRADITIONAL CHINESE DAIM NTAWV THOV KOM TAU TXAIS KEV KHO MOB

Cov tshuaj suav tshuaj suav tshuaj Eucommia ulmoides, Cuscuta phev, thiab Drynariae rhizome extracts txhim kho glucocorticoid-induced osteoporosis los ntawm inhibiting osteoclastogenesis los ntawm PI3K / Akt txoj kev [14]. Epimedium tseem tuaj yeem tswj hwm ntau yam kab mob los ntawm PI3K / Akt [15]. Molecular docking cov txiaj ntsig qhia tau tias MMP-9 thiab suchilac-

Cov molecules me me xws li suab nrov, quercetin, luteolin, thiab kaempferol muaj zog khi zoo. Cov kev tshawb fawb zaum kawg no qhia tau hais tias MMP-9 yog ib qho tseem ceeb ntawm cov protein uas cuam tshuam rau cov pob txha metabolic kab mob xws li OP [16]. Kev tshawb fawb qhia tau hais tias velvet antler polysaccharide thiab peptide extracts txhawb MAKP thiab MMP-9 signaling.

txoj kev inhibits pob txha resorption nyob rau hauv siab turnover osteoporosis [17]. Ligands quercetin thiab VitE tuaj yeem tswj hwm tag nrho cov pob txha pob txha kom tsawg dua thiab tiv thaiv kev mob pob txha los ntawm kev tswj cov kev qhia ntawm autophagy thiab apoptosis proteins xws li LC3, beclin1 thiab caspase 3 [18]. Luteolin muaj ntau lub hom phiaj thiab ntau txoj kev cuam tshuam hauv kev kho mob pob txha, tuaj yeem txo cov pob txha poob hauv OVX nas, thiab tuaj yeem txhawb kev sib txawv ntawm osteogenic ntawm BMSCs los ntawm kev tswj cov haujlwm ntawm PI3KAkt signaling pathway19].

Raws li molecular docking, nws tau pib pom tias cov khoom sib xyaw me me uas muaj nyob hauv cov tshuaj suav tshuaj suav tshuaj tuaj yeem kho thiab tiv thaiv OP los ntawm ntau lub hom phiaj thiab ntau txoj hauv kev.

Piv txwv li, xws lilactone, quercetin, luteolin, kaempferol thiab lwm yam khoom xyaw yog cov khoom tseem ceeb ntawm Drynariae, Epimedium thiab Cistanche deserticola, thiab lwm yam xws li AKT1, ERS2, MMP-9, AR, mTOR thiab lwm yam yog qhov tseem ceeb. lub hom phiaj ntawm qhov tseem ceeb me me molecules. point, tejzaum nws kho los ntawm nws txoj kev

thiab tiv thaiv OP. Kev soj ntsuam los ntawm kev sim tshuaj hauv network muab lub hauv paus theoretical rau kev tshawb fawb ntxiv cov tshuaj suav tshuaj suav tshuaj Drynariae, Epimedium, thiab Cistanche deserticola hauv kev kho OP, tab sis ntxiv elucidation ntawm cov txheej txheem metabolic tshwj xeeb tseem yuav tsum tau kuaj xyuas.

Cov ntaub ntawv:

[1] Hu Xu, Lian Xu, Zhang Qi, et al. Kev tshawb fawb ntawm cannabinoid hom 2 receptor agonist kev ua ntawm Rehmannia glutinosa thiab nws cov kev tswj hwm ntawm cov pob txha metabolism [J]. Suav tshuaj ntsuab, 2022, 53(20): 6481-6491.

[2] Wu D, Cline-Smith A, Shashkova E, et al. T-Cell Mediated Inflamation hauv Postmenopausal Osteoporosis [J]. Pem Hauv Ntej Immunol, 2021, 12: 687551. [3] Ma Jiangtao, Wan Lei, Huang Hongxing. Kev tshawb nrhiav cov txheej txheem ntawm kev txiav txim ntawm Drynaria fortunei hauv kev kho mob pob txha raws li network pharmacology [J]. Suav Journal of Osteoporosis, 2020, 26(4): 490-496.

[4] Huang JW, Yang WS, et al. Kev tshawb nrhiav cov txheej txheem ntawm kev ua ntawm Drynaria fortunei hauv kev kho mob pob txha raws li kev siv tshuaj hauv network thiab hauv vitro cell thwmsim Kawm txog cov txheej txheem ntawm kev ua ntawm cov khoom xyaw nquag ntawm "Sanqi-Gu Sui Bu" tshuaj rau kev kho mob pob txha [J] . Suav Journal of Traditional Chinese Medicine, 2023, 48(4): 1087-1097.

[5]Bi Z, Zhang W, Yan X. Anti-inflammatory thiab immunoregulatory teebmeem ntawm icariin thiab icaritin [J]. Biomed Pharmacother, 2022, 151: 113180.

[6] Dai Z. Kawm txog kev tiv thaiv cov nyhuv thiab cov txheej txheem ntawm Rhizoma Drynariae -Epimedium Formula rau Osteoarthritis hauv nas[J]. Contrast Media Mol Imaging, 2022, 2022: 2869707.

[7] Zhao JF, Xu JY, Xu YE, et al. High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathway to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis [J]. Plaub Hlis 11, 2020, 11:13 teev tsaus ntuj. [8] Li M., Zhang ND, Wang Y, et al. Kev tswj hwm osteogenesis cuam tshuam ntawm icariin, timosaponin B II thiab ferulic acid los ntawm cov tshuaj suav tshuaj suav tshuaj ntawm UMR-106 osteoblastic cells thiab osteoblasts hauv neonatal nas calvaria cultures [J].J ,2016,185:120-131