Lipoamide Alleviates Oxidized Fish Oil-Induced Host Inflammatory Response Thiab Oxidative Damage hauv Oviduct Ntawm Laying Hens Part 1
Jul 28, 2023
Ntses roj (FO) yog ib qho tseem ceeb ntawm lipids hauv cov khoom noj khoom haus thiab aquafeeds. Txawm li cas los xij, qhov teeb meem cuam tshuam ntawm oxidized ntses roj (OFO) ntawm tus tswv tsev metabolism thiab kev noj qab haus huv tsis tau paub meej. Tsis tas li ntawd, lipoamide (LAM) tau kawm dav dav los ua tus neeg sawv cev rau kev txo ntau yam kab mob cuam tshuam nrog oxidative cuam tshuam. Yog li, txoj kev tshawb fawb tam sim no tshawb xyuas qhov cuam tshuam ntawm LAM hauv kev txo qis OFO vim kev poob qis hauv kev ua me nyuam thiab kev puas tsuaj oxidative rau lub oviduct hauv nteg qe. Peb tsim 1 feem pua ntawm FO qauv tshiab, 1 feem pua OFO qauv, thiab LAM qauv nrog 1 feem pua OFO (OFO ntxiv LAM) ntxiv ntawm 100 mg/kg los tshawb txog cov nyhuv antioxidant ntawm LAM. Ntawm no, cov txiaj ntsig no tau raug soj ntsuam los ntawm kev yug me nyuam, kev tiv thaiv kab mob, tshuaj estrogen, thiab cov tshuaj tiv thaiv antioxidant ntawm cov qauv ntshav, nrog rau cov follicles thiab cov tshuaj tiv thaiv antioxidant ntawm oviducts. Los ntawm cov txiaj ntsig, piv nrog FO pawg, OFO txo qis qis qis qis, nce cov ntsiab lus ntawm tag nrho cov protein (TP) thiab cov kab mob inflammatory [mob necrosis factor (TNF-), interleukin (IL)-6, IL-8, thiab interferon (INF- )], thiab txo cov concentration ntawm cov tshuaj tiv thaiv oxidation [tag nrho cov antioxidant (T-AOC), tag nrho superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), glutathione reductase (GR), catalase (CAT), thiab hydroxyl radical scavenging activity (HRSA)] hauv cov qauv ntshav, nrog rau txo cov qib ntawm cov tshuaj tiv thaiv oxidation hauv cov ntaub so ntswg oviduct (p < 0 .05). Ntawm qhov kev ceeb toom, kev ntxiv ntawm LAM tuaj yeem ua rau muaj txiaj ntsig zoo, txhim kho cov qib ntawm cov tshuaj tiv thaiv kab mob hauv lub cev (IgA, IgG, thiab IgM), cov tshuaj estrogen [progesterone (P) thiab estradiol (E2)], thiab cov tshuaj tiv thaiv kab mob (T- AOC, T-SOD, GSH-Px, GSH, GR, CAT, thiab HRSA) thiab txo cov concentrations ntawm cov ntshav cytokines (TNF-, IL-6, IL-8, thiab INF-) hauv tso hens tom qab OFO kev tswj hwm (p <0.05). Tsis tas li ntawd, LAM tuaj yeem nce cov ntsiab lus ntawm cov tshuaj tiv thaiv antioxidant (p <0.05) hauv oviducts thiab txhim kho lub peev xwm zais cia ntawm lub tsev menyuam. Ua ke, OFO ua rau tus tswv tsev metabolic tsis ua haujlwm, oxidative puas tsuaj, uterine morphological abnormalities, thiab hloov pauv hauv zes qe menyuam ua haujlwm. Cov txiaj ntsig no tau qhia tias kev tswj hwm LAM tuaj yeem txo tus tswv tsev metabolic tsis ua haujlwm thiab ua rau muaj kev puas tsuaj, thiab tom qab ntawd ameliorate oxidative kev puas tsuaj hauv oviduct vim OFO, thaum kawg txhim kho kev ua me nyuam.
Glycoside ntawm cistanche tuaj yeem ua rau muaj kev ua haujlwm ntawm SOD hauv plawv thiab daim siab cov ntaub so ntswg, thiab txo cov ntsiab lus ntawm lipofuscin thiab MDA hauv txhua cov ntaub so ntswg, tshem tawm ntau yam reactive oxygen radicals (OH-, H₂O₂, thiab lwm yam) thiab tiv thaiv DNA puas. los ntawm OH-radicals. Cistanche phenylethanoid glycosides muaj peev xwm tshem tawm cov dawb radicals, muaj peev xwm txo tau ntau dua li cov vitamin C, txhim kho cov haujlwm ntawm SOD hauv cov phev ncua, txo cov ntsiab lus ntawm MDA, thiab muaj qee yam kev tiv thaiv ntawm cov phev ua haujlwm. Cistanche polysaccharides tuaj yeem txhim kho kev ua haujlwm ntawm SOD thiab GSH-Px hauv erythrocytes thiab ntsws cov ntaub so ntswg ntawm cov nas senescent sim los ntawm D-galactose, nrog rau txo cov ntsiab lus ntawm MDA thiab collagen hauv lub ntsws thiab ntshav, thiab nce cov ntsiab lus ntawm elastin, muaj Cov nyhuv scavenging zoo ntawm DPPH, ncua lub sijhawm ntawm hypoxia hauv cov nas senescent, txhim kho cov haujlwm ntawm SOD hauv cov ntshav, thiab ncua lub physiological degeneration ntawm lub ntsws hauv kev sim cov nas nrog cellular morphological degeneration, kev sim tau pom tias Cistanche muaj peev xwm antioxidant zoo. thiab muaj peev xwm los ua ib qho tshuaj tiv thaiv thiab kho cov kab mob ntawm daim tawv nqaij laus. Nyob rau tib lub sijhawm, echinacoside hauv Cistanche muaj lub peev xwm tseem ceeb txhawm rau txhawm rau DPPH dawb radicals thiab muaj peev xwm tshem tawm cov pa oxygen reactive thiab tiv thaiv dawb radical-induced collagen degradation, thiab kuj muaj kev kho zoo ntawm thymine dawb radical anion puas.
Nyem rau ntawm Cistanche Tablets Cov txiaj ntsig
【Yog xav paub ntxiv:george.deng@wecistanche.com / WhatApp:86 13632399501 】
Ntsiab lus: lipoamide, oxidized ntses roj, tiv thaiv oxidation, oviduct, tso hens
Taw qhia
Thaum lub sij hawm txheej yug me nyuam, teeb meem, xws li ib tug sai poob nyob rau hauv lub qe ntau lawm tus nqi ntawm cov laus nteg qe thiab tsis zoo ntawm lig kev lag luam qe, yog txwv ntau lawm efficiency. Nws feem ntau lees paub tias qhov poob ntawm fertility feem ntau cuam tshuam los ntawm kev laus ntawm lub cev xeeb tub (1). Feem ntau ntawm cov teeb meem kev noj qab haus huv ntsig txog kev laus yog cuam tshuam nrog kev ntxhov siab oxidative ntau dhau thiab mob hauv lub cev. Nws yog hypothesized tias lub ntsiab yog vim li cas rau poob qe ntau npaum li cas nyob rau hauv cov laus nteg qe yog oxidative puas thiab inflammatory teb rau lub oviductal mucosa induced los ntawm lub physiological kev nyuaj siab ntawm lub sij hawm ntev ntau lawm, oxidized rog nyob rau hauv cov zaub mov, thiab aflatoxin. (AFB) commulation poisoning (2). Ib txoj kev tshawb fawb uas kav ntev li 23 xyoos tau pom tias kev poob vim salpingitis nyob rau hauv cov laus nteg hens suav txog 1-8 feem pua ntawm tag nrho cov kev poob ntawm kev ua liaj ua teb (3). Qhov no tuaj yeem txhais tau tias kev noj qab haus huv ntawm oviduct zoo heev txwv qhov kev siv ntawm cov qub nteg qe. Yog li ntawd, alleviating oviductal oxidative kev nyuaj siab, txo qhov tshwm sim ntawm salpingitis, thiab tswj oviduct noj qab haus huv tej zaum yuav yog ib qho tseem ceeb cov lus qhia los txhim kho qe ntau lawm nyob rau hauv cov laus nteg qe.
Raws li peb txhua tus paub, cov roj ntses (FO) muaj qhov tshwj xeeb lom neeg ua haujlwm ntawm kev txhawb nqa cov metabolism ntawm saturated fatty acids hauv vivo. Txawm li cas los xij, vim nws qib siab tsis txaus ntseeg, FO yog qhov cuam tshuam rau oxidation, thiab cov teeb meem ntawm oxidized ntses roj (OFO) ntawm cov tsiaj txhu tau nthuav dav. Ntau cov kev tshawb fawb tau pom tias kev pub OFO rau cov tsiaj sim tuaj yeem ua rau muaj cov pa oxygen ntau dhau (ROS), uas tuaj yeem ua rau MDA thiab noj SOD, tom qab ntawd ua rau muaj kev ntxhov siab oxidative (4-7). Kev noj OFO los ntawm cov tsiaj yuav ua mob rau kev loj hlob thiab kev muaj peev xwm antioxidant ntawm cov tsiaj lub cev, txo qhov tsis kam ntawm cov tsiaj mus rau cov kab mob, thiab txo qhov zoo ntawm cov khoom tsiaj.
Lipoic acid (LA), ib qho khoom noj tseem ceeb uas ua rau qeeb lossis kho oxidative puas tsuaj thiab nthuav tawm cov haujlwm muaj zog antioxidant (8), suav tias yog tus neeg sawv cev zoo hauv kev tiv thaiv lossis kho qee yam kab mob ntawm kev laus (9). Hauv kev sim ntawm polycystic zes qe menyuam syndrome cov poj niam, LA tau pom tias yuav ua rau muaj cov tshuaj tiv thaiv antioxidant hauv cov menyuam yaus thiab txhim kho kev ua haujlwm ntawm cov menyuam yaus (10). Hauv cov nqaij qaib, ntau qhov kev tshawb fawb tau pom tias LA nce qhov kev ua haujlwm ntawm cov enzyme antioxidant thiab tag nrho cov tshuaj tiv thaiv antioxidant (T-AOC) hauv cov ntshav thiab daim siab cov qauv, nrog rau txo qis lipid peroxidation hauv vivo raws li kev noj zaub mov zoo hauv broilers (11). —13). Lipoamide (LAM) yog qhov tseem ceeb tshaj plaws nruab nrab amide ntawm LA, thiab ob lub tebchaw no muaj cov qauv zoo sib xws thiab muaj peev xwm lom neeg (14, 15). Cov kev tshawb fawb tau qhia tias LAM yog ib qho antioxidant hauv vitro (16), thiab LAM muaj cov nyhuv antioxidant ntau dua li LA (17, 18). Hou et al. pom tias LAM tuaj yeem tiv taus oxidative kev nyuaj siab-mediated neuronal cell puas. Dhau li ntawm tib qhov concentration, cov nyhuv antioxidant ntawm LM tau zoo dua li LA (19). Hais txog kev tiv thaiv kev tiv thaiv ntawm LM zoo dua LA, nws yuav yog vim LM muaj lipid solubility ntau dua, yog li nws muaj peev xwm hloov mus rau lub cev ib puag ncig tshaj li ntawm LA (20, 21).
Oxidative stress yog lub ntsiab ua rau degeneration ntawm oviduct muaj nuj nqi thiab salpingitis nyob rau hauv nteg hens (22). LAM, raws li lub zog antioxidant, tuaj yeem muaj peev xwm tiv thaiv oxidative puas tsuaj. Txawm li cas los xij, seb LAM puas muaj cov txiaj ntsig los txo cov kev puas tsuaj oxidative hauv oviduct tseem tsis tau paub. Yog li ntawd, peb txoj kev tshawb fawb tau ua los tsim ib qho qauv ntawm OFO-vim kev ntxhov siab hauv cov qub nteg qe thiab tom qab ntawd tshawb xyuas qhov tsis zoo ntawm LAM mitigation ntawm oxidative kev nyuaj siab los tsim cov khoom noj ntxiv kom txo qis oviduct o thiab oxidation hauv cov hens.
Cov khoom siv thiab cov txheej txheem
Tag nrho cov txheej txheem cuam tshuam txog kev siv tsiaj hauv txoj kev tshawb fawb no tau pom zoo los ntawm Pawg Saib Xyuas Kev Saib Xyuas Tsiaj thiab Siv Khoom ntawm Suav Agricultural University.
Tsiaj Husbandry and Experiment Design
Ob qhov kev sim tau ua sib cais hauv qhov kev tshawb fawb no. Kev sim 1 tau tsim los tshawb xyuas cov txiaj ntsig ntawm kev noj zaub mov ntxiv ntawm LAM ntawm cov ntsuas kev ua haujlwm ntawm kev yug me nyuam, xws li qe-ntev tus nqi, qe hnyav, qe ntau lawm, thiab pub-rau-qe piv ntawm cov qub nteg qe. Ua ntej, tag nrho ntawm 60 kev lag luam tso hens ntawm Peking Red strain (Yukou Poultry Co., Ltd. ntawm Beijing, Tuam Tshoj) thaum muaj hnub nyoog 106 lub lis piam nrog kev ua haujlwm zoo sib xws tau muab faib ua ob pawg kev kho mob (CON) thiab ntxiv 100 mg/kg LAM pawg (LAM). Txhua pab pawg muaj 15 replicates (ob nteg hens ib replicate) nyob rau hauv 15 txawv tawb (ob tug noog ib lub tawb). Cages (H 45 × W 45 × D 45 cm) tau nruab nrog ib lub txiv mis haus dej thiab ib qho khoom noj sab nrauv uas nthuav qhov ntev ntawm lub tawb. Cov qaib tau loj hlob nyob rau hauv ib qho chaw kaw, qhov cua, thiab cov pa hauv tsev nrog 16 h-teeb thiab 55 feem pua ntawm cov av noo nyob nruab nrab. Pub thiab dej tau muab ad libitum thaum lub sijhawm sim tag nrho. Rau 2 lub lis piam ntawm kev pub noj ua ntej, tag nrho cov kev kho mob tau noj cov pob kws-soybean noj zaub mov rau cov hens lig. Los ntawm 108 lub lis piam (lub lim tiam 1 ntawm qhov kev sim), txhua qhov kev kho mob tau noj cov zaub mov sib raug thiab pib lub sijhawm 16- lub lis piam soj ntsuam mus txog rau thaum xaus ntawm 123 lub lis piam (lub lim tiam 16 ntawm qhov kev sim). Cov qe qe tso txhua lub limtiam, qe hnyav, thiab noj zaub mov tau sau tseg rau 16 lub lis piam, thiab qe qe thiab pub-rau-qe piv tau suav.
Kev sim 2 tau tsim los tshawb xyuas cov txiaj ntsig ntawm LAM ntawm kev ua me nyuam, kev tiv thaiv kab mob ntshav, inflammatory thiab hormonal indexes, thiab antioxidant indices ntawm cov ntshav thiab uterine ib feem ntawm cov laus nteg qe nyob rau hauv oxidative kev nyuaj siab. Los ntawm 124 lub lis piam (lub lim tiam 17 ntawm qhov kev sim), 30 hens nyob rau hauv pawg CON hauv kev sim 1 tau muab faib ua ob pawg thiab ntxiv nrog 1 feem pua ntawm FO tshiab thiab 1 feem pua OFO, feem. Tus nqi peroxide ntawm FO tshiab thiab OFO yog 3.65 thiab 184.54 meq / kg, feem. Txhua qhov kev kho mob muaj 15 replicates nrog ib tug noog ib replicate, muab tso rau hauv tib lub tawb. Tsis tas li ntawd, 15 nteg hens nyob rau hauv pab pawg LAM hauv kev sim 1 tau xaiv xaiv thiab ntxiv nrog 1 feem pua OFO (OFO ntxiv rau LAM). Thaum kawg ntawm 127 lub lis piam ntawm hnub nyoog (lub lim tiam 20 ntawm qhov kev sim), qhov kev ua me nyuam ntawm peb pawg kho mob tau sau tseg. Cov zaub mov basal pob kws-soybean noj zaub mov tau tsim los ua kom tau raws li cov cai ntawm Peking Red nteg hens (NYT33-2004) (Table 1). Thaum lub sijhawm sim, cov hens tau pub ob zaug ib hnub.
Qauv sau thiab kho
Thaum kawg ntawm lub sijhawm sim (127 lub lis piam), ib tus noog ze rau qhov hnyav nruab nrab raug xaiv rau txhua qhov rov ua dua. Cov noog uas xaiv tau raug ntsuas thawj zaug. Tom qab ntawd, cov ntshav tis venous tau sau, thiab cov ntshav tau txais los ntawm centrifugation ntawm cov qauv ntshav ntawm 3, 000 r / min rau 15 min ntawm 4◦C thiab khaws cia ntawm −80◦C mus txog rau kev soj ntsuam ntxiv. Tom qab sau ntshav, cov noog tau muab txi thiab txiav. Lub tsev menyuam tau sau los ntawm ib txhij ntxuav nrog txias sterile PBS kom tshem tawm cov impurity txuas thiab maj mam muab txhuam. Cov qauv hauv tsev menyuam tau diluted nrog rau cuaj lub sij hawm ntim ntawm sterile ice-txias ib txwm saline (0.9 feem pua) raws li tus qauv hnyav thiab tom qab ntawd homogenized siv lub tes tuav iav homogenizer. Cov ntaub so ntswg supernatants tau sau los ntawm centrifuging ntawm 3,500 × g rau 10 min ntawm 4◦C, thiab cov concentration ntawm cov protein tau txiav txim los ntawm BCA protein assay cov khoom raws li cov neeg tsim khoom qhia (Pierce, Rockford, IL) thiab khaws cia ntawm −80◦ C rau kev kawm ntxiv. Thaum kawg, lub zes qe menyuam thiab oviducts raug muab cais, yees duab, thiab hnyav, qhov ntev ntawm cov oviducts raug ntsuas, tus naj npawb ntawm cov follicles sib txawv raug suav, ces cov ntaub ntawv tau raug kaw.
Serum Biochemical, Immune, Inflammatory, thiab Hormone Parameters
Cov qib ntawm tag nrho cov protein (TP), albumin (ALB), immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), qog necrosis factor (TNF-), interleukin 1 (IL-1 ) , interleukin 6 (IL-6), thiab interferon (IFN-) hauv cov ntshav tau ntsuas nrog cov khoom siv (Nanjing Jiancheng Bioengineering Institute, Nanjing, Suav). Cov theem ntawm estradiol (E2) thiab progesterone (P) hauv cov ntshav tau txiav txim siab nrog cov khoom siv hluav taws xob ua lag luam (RIA) los ntawm cov chaw tsim khoom cov lus qhia (Nanjing Jiancheng Bioengineering Institute, Nanjing, Suav).
Antioxidant Assay of Serum thiab Uterus
Cov tshuaj tiv thaiv kab mob hauv tsev menyuam thiab cov tshuaj tiv thaiv kab mob hauv tsev menyuam tau kuaj pom siv cov khoom siv coj mus muag (Nanjing Jiancheng Bioengineering Institute, Nanjing, Suav). Cov concentrations ntawm tag nrho cov antioxidant (TAOC), tag nrho superoxide dismutase (T-SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-Px), glutathione (GSH), hydroxyl radical scavenging kev ua (HRSA), thiab malondialdehyde (MDA) hauv cov ntshav thiab uterine ib feem raug ntsuas los ntawm cov chaw tsim khoom cov lus qhia. Cov txiaj ntsig tau normalized rau cov protein ntau hauv txhua lub tsev menyuam homogenate.
Hematoxylin-Eosin (H & E) Staining thiab Histopathology Analyzes
Ntau qhov chaw ntawm lub tsev menyuam tau sau los ntsuas kev puas tsuaj histopathological. Tom qab ntawd, uterine cais tau ntws nrog PBS, thiab lub tsev menyuam tau muab cais ua ob ntu. Ib feem yog snap-khov hauv cov kua nitrogen thiab tom qab ntawd muab khaws cia ntawm −80◦C hauv lub tub yees rau kev soj ntsuam tom qab. Lwm cov ntaub so ntswg tau kho nyob rau hauv 4 feem pua paraformaldehyde, suav nrog paraffin, txiav rau hauv micro-seem ntawm 5µm, mounted rau iav slides, thiab thaum kawg stained nrog hematoxylin (Solarbio, Beijing, Suav teb) thiab eosin Y tov (Solarbio, Beijing, Suav teb) . Tom qab ntawd, hematoxylin thiab eosin (H&E) stained paraffin seem tau saib nyob rau hauv ci ntsa iab ntawm Zeiss Axio Imager microscope raws li tau hais dhau los. Microscopic plab hnyuv puas tau pom nyob rau hauv cov duab uas siv cov cuab yeej ntsuas ntawm Case Viewer software ntawm × 200 magnification.
Kev txheeb cais
Hauv kev sim ib qho, cov ntaub ntawv tseem ceeb ntawm kev yug me nyuam tau raug tshuaj xyuas los ntawm qhov tsis sib xws t-test siv SPSS 25.0 (SPSS Inc., Chicago, IL, USA). Cov txiaj ntsig ntawm tus lej raug qhia raws li qhov ntsuas, nrog p < 0.05 suav tias yog qhov tseem ceeb.
Hauv kev sim 2, thawj qhov kev ua me nyuam yug me nyuam, kev tiv thaiv kab mob, kab mob, thiab cov tshuaj hormones, thiab cov tshuaj tiv thaiv antioxidant tau ua los ntawm kev siv ANOVA ib-txoj kev muaj nrog SPSS 25.0 (SPSS Inc., Chicago, IL, USA). Cov kev kho mob txhais tau tias yog sib cais los ntawm Duncan ntau yam kev ntsuam xyuas ntawm p < 0.05 theem tseem ceeb.
TSEEM CEEB
Qhov cuam tshuam ntawm LAM ntawm Kev Ua Haujlwm ntawm Laying hens Hauv Kev Pub Noj
Raws li pom hauv kev sim 1 (Table 1), piv nrog rau pawg tswj hwm, tsis muaj qhov cuam tshuam tseem ceeb ntawm kev noj zaub mov ntxiv nrog LAM ntawm lub qe-ntev tus nqi, qe hnyav, qe qe, pub noj, nteg qe qe, thiab pub / qe piv. hauv kev sim ntawm 1–16 lub lis piam (p > 0.05). Txawm li cas los xij, muaj qhov cuam tshuam zoo ntawm LAM ntawm kev ua me nyuam ntawm cov laus nteg qe nyob rau hauv ib txwm pub.
Qhov cuam tshuam ntawm LAM ntawm Kev Ua Tau Zoo ntawm Kev Tso Hniav Hauv Kev Nyuaj Siab Oxidative
Hauv kev sim 2 (Table 2), piv nrog FO pab pawg, OFO pawg muaj qhov qis qis qis qis (p < 0.05) thiab txo qis hauv qe qe (p > 0 05). Tsis muaj qhov sib txawv tseem ceeb hauv qe qhov hnyav, kev noj zaub mov noj, tso qhov hnyav ntawm tus qaib, thiab pub-rau-qe piv (p > 0.05). Ntawm qhov tod tes, piv nrog pab pawg OFO, cov qe qe thiab qe ntau lawm hauv pab pawg OFO ntxiv rau LAM tau nce ntxiv (p <0.05), tab sis tsis muaj qhov hloov pauv loj hauv lub qe hnyav, noj zaub mov, tso hens. qhov hnyav, thiab pub / qe piv (p> 0.05).
Qhov cuam tshuam ntawm LAM ntawm Cov Khoom Siv Biochemical Parameters, Immunoglobulin Levels, Inflammatory Cytokines, thiab Antioxidant Capacity ntawm Tsob Nroj Los Ntawm Oxidative Stress
Raws li pom hauv daim duab 1, piv nrog FO pawg, qib ntawm TP tau nce ntau thiab cov ntsiab lus ntawm IgM tau txo qis hauv OFO pawg (p < 0.05), tab sis muaj tsis muaj qhov sib txawv tseem ceeb hauv cov ntsiab lus ntawm ALB, IgA, thiab IgG (p > 0.05). Hauv pab pawg OFO ntxiv rau LAM, cov ntsiab lus ntawm IgG, IgA, thiab IgM tau siab dua li ntawm pawg OFO (p <0.05).
Raws li pom hauv daim duab 2, piv nrog FO pawg, cov ntsiab lus ntawm TNF- , IL-1 , IL-6, thiab IFN- tau nce ntau hauv pawg OFO (p < 0 05). Hauv pab pawg OFO ntxiv rau LAM, cov ntsiab lus ntawm TNF- , IL-1 , IL-6, thiab IFN- tau txo qis heev dua li hauv pawg OFO (p < 0.{{ 18}}5). Tsis tas li ntawd, (Daim duab 3) tsis muaj qhov sib txawv tseem ceeb hauv qib P thiab E2 hauv pawg OFO piv nrog FO pawg, tab sis muaj qhov sib txawv ntawm ob pawg (p > 0.{{29} }5). Piv nrog rau pab pawg OFO, qib P thiab E2 tau nce ntau hauv OFO ntxiv rau LAM pawg (p <0.05). Qhov tseem ceeb (Daim duab 4), piv nrog FO pawg, cov ntsiab lus ntawm T-AOC, T-SOD, GSH-Px, GSH, GR, CAT, thiab HRSA tau poob qis, thiab qib ntawm MDA tau nce ntau hauv OFO. pab pawg (p <0.05). Ntawm qhov tod tes, cov theem ntawm T-AOC, T-SOD, GSH-Px, GSH, GR, CAT, thiab HRSA tau nce siab dua hauv OFO ntxiv rau LAM pawg dua li hauv pab pawg OFO, thaum MDA tau qis dua nyob rau hauv pawg OFO (p <0.05).
Qhov cuam tshuam ntawm LAM ntawm Uterine Part Morphology thiab Antioxidant Peev Xwm ntawm Laying Hens Nyob rau hauv Oxidative Stress
Tubular qog nyob rau hauv pawg FO yog sparsely ntim rau hauv lub taub hau ntawm mucosal quav, thiab nruj nreem ntim rau hauv lub hauv paus ntawm mucosal folds (Daim duab 5A). Hauv pab pawg OFO, cov qog qog nqaij hlav tau ntim rau hauv cov mucosal folds (Daim duab 5B). Piv nrog rau pab pawg OFO, cov qog qog tau nruj nruj rau hauv cov mucosal quav hauv OFO ntxiv rau LAM pab pawg, thiab cov qog yog kab nrog cov hlwb uas muaj cov eosinophilic granules zoo (Daim duab 5C). Qhov nruab nrab qhov ntev ntawm qhov sib txawv tau ntsuas (Daim duab 5D). Qhov nruab nrab qhov ntev ntawm pawg OFO tau luv dua li cov hauv pawg FO, tab sis tsis muaj qhov sib txawv ntawm ob pawg (p > 0.05). Txawm li cas los xij, qhov nruab nrab qhov ntev ntawm pawg OFO ntxiv rau LAM tau ntev dua li ntawm pawg OFO (p <0.05).
Cov nyhuv ntawm FO, OFO, thiab OFO ntxiv rau LAM ntawm uterine feem antioxidant muaj peev xwm yog pom nyob rau hauv daim duab 5E-L. Piv nrog rau pawg FO, cov ntsiab lus ntawm T-AOC, T-SOD, GSH-Px, GSH, GR, CAT, thiab HRSA tau poob qis, thiab MDA cov ntsiab lus tau nce ntau hauv pawg OFO (p < {{ 4}} {8}}5). Piv nrog rau pawg OFO, cov ntsiab lus ntawm T-AOC, TSOD, GSH-Px, GSH, GR, CAT, thiab HRSA tau nce ntau, thiab MDA cov ntsiab lus tau txo qis hauv OFO ntxiv rau LAM pawg (p <0.05).
Cov nyhuv ntawm LAM ntawm Ovary thiab Oviduct ntawm Laus Laying Hens Nyob rau hauv Oxidative Stress
In terms of the number of follicles (Figures 6A–H), the number of dominant follicles in the OFO group is significantly decreased compared with the FO group (p < 0.05). Compared with the OFO group, the number of dominant follicles (P < 0.05) and total number of follicles (0.05 < P < 0.1) increased in the OFO + LAM group, while there were no significant changes in another follicle number (p>{{0}}.05). Raws li pom hauv daim duab 6I, tsis muaj qhov sib txawv ntawm qhov ntev ntawm oviduct ntawm peb pawg, tab sis qhov ntev oviduct hauv OFO ntxiv rau LAM pawg tau nce mus rau qee qhov piv nrog OFO pawg (p> 0.05).
Kev sib tham
Cov teebmeem ntawm OFO ntawm cov tsiaj txhu tau kawm los ntawm ntau tus kws sau ntawv, thiab cov kev tshawb fawb feem ntau tau pom tias OFO ua rau cov tsiaj tsis zoo thiab txo qis kev zom zaub mov thiab kev siv cov as-ham (5, 23). Tshwj xeeb, nyob rau hauv cov nqe lus ntawm oxidative kev nyuaj siab, qhov sib ntxiv ntawm 15 feem pua OFO rau cov khoom noj ua rau oxidative kev puas tsuaj nyob rau hauv ntau yam tsiaj kab mob, feem ntau yog los ntawm kev tso tawm ROS, ntau ntau lawm ntawm MDA, txo cov antioxidant enzymes, xws li SOD, thiab oxidative kev nyuaj siab. hauv ntau lub cev thiab cov ntaub so ntswg (4-7). Oxidative stress yog txhais tias yog qhov tsis sib xws ntawm kev tsim cov dawb radicals thiab lawv tshem tawm. Qhov kev tsis txaus ntseeg no ua rau muaj kev puas tsuaj ntawm cov biomolecules tseem ceeb thiab cov hlwb, nrog rau kev cuam tshuam rau tag nrho cov kabmob (24). Nkauj et al. (25) pom tias kev noj zaub mov 6 feem pua OFO inhibited kev loj hlob thiab ua kom cov plab hnyuv siab rau hauv cov ntses. Txoj kev tshawb fawb tam sim no tau lees paub nrog cov ntawv tshaj tawm yav dhau los tias cov khoom noj uas tau ntxiv nrog OFO nce cov ntsiab lus ntawm qhov mob o (TNF- , IL-1 , IL-6, thiab IFN- ), antioxidant enzymes (T-AOC, T-SOD, GSHPx, GSH, GR, CAT, thiab HRSA), txo MDA cov ntsiab lus thiab nws thiaj li coj mus rau qhov txo qis ntawm cov qe qe ntawm cov qub nteg qe piv nrog cov FO tshiab. Nws kuj tseem sawv cev tias OFO tuaj yeem ua tiav cov qauv kev ntxhov siab oxidative hauv cov qub nteg qe.
Lipoic acid yog sulfur-muaj fatty acid, uas tuaj yeem tshem tawm cov pa dawb radicals sai sai thaum nws nkag mus rau hauv lub cev thiab muaj kev ua haujlwm zoo antioxidant. LAM, carboxylic acid derivative ntawm LA, maj mam dhau los ua lwm txoj hauv kev rau LA vim tias nws muaj cov haujlwm biomedical zoo dua thiab tsim nyog siv rau tib neeg ntau dua li LA (21). Ntau qhov kev soj ntsuam tau pom tias LAM yog cov muaj zog antioxidant ntau dua li LA hais txog kev puas tsuaj oxidative (15), xws li hauv kev tiv thaiv macrophage zoo li cov hlwb los ntawm oxidative raug mob (26), thiab cov hlwb neuronal los ntawm glutamate-induced cell raug mob (27 ). Txawm li cas los xij, nws tsis paub meej tias qhov sib ntxiv ntawm LAM rau kev noj haus yuav ua rau cov hens raug mob. Yog li ntawd, peb tau ntxiv LAM-muaj pub rau cov qub nteg qes uas nyob rau hauv ib txwm tsim thaum xub thawj, thiab pom tias LAM tsis cuam tshuam rau qe ntau lawm thiab kev tsim tawm, thiab txawm tias muaj qee qhov txiaj ntsig zoo.
Cov qe ntau lawm yog ib qho tseem ceeb qhia txog kev tsim qe qe. Nyob rau theem tom ntej ntawm kev tsim qe, qhov txo qis hauv qe qe tuaj yeem cuam tshuam rau kev lag luam ntawm cov neeg ua liaj ua teb. Kev tswj hwm kev noj zaub mov tuaj yeem txhim kho lub qe ntau lawm thiab txuas ntxiv lub sij hawm qe-ntev ntawm nteg qe. Ntau cov kev tshawb fawb tau pom tias kev noj zaub mov ntxiv nrog cov tshuaj antioxidants tuaj yeem txhim kho qe qe hauv cov hens. Piv txwv li, Kothari et al. pom tias kev noj zaub mov ntxiv ntawm fermented ntoo thuv koob extract nce qe ntau lawm thiab pub noj rau tag nrho lub sijhawm sim (28). Liu et al. (29) qhia tias lub qe-ntws tus nqi muaj ib tug quadratic correlation nrog cov theem ntawm quercetin thiab tau maximized los ntawm qib supplementation ntawm 0.2 g / kg ntawm kev noj haus. Raws li cov tshuaj antioxidant muaj zog, peb ntseeg tias LAM muaj txiaj ntsig zoo rau kev tsim cov qe hauv cov hens, thiab zoo sib xws, peb cov kev sim tau pom tias kev noj zaub mov ntxiv ntawm LAM tau txhim kho cov qe qe thiab qe ntau lawm hauv cov laus nteg qe. Tsis tas li ntawd, ib txoj kev tshawb nrhiav pom tias qhov sib ntxiv ntawm LA rau cov khoom noj tau nce lub qe qe, tab sis qhov sib txawv tsis tseem ceeb (30). Ua ke, cov txiaj ntsig tau pom tias LAM yuav ua tau zoo dua LA hauv kev tsim cov nqaij qaib.
Qe ntau lawm yog qhov tseem ceeb txwv qhov kev ua tau zoo ntawm kev siv qe thaum lub sij hawm qe qe qe, thaum lub zes qe menyuam thiab oviduct kev laus yog qhov laj thawj tseem ceeb rau kev poob qe (31, 32). Oxidative stress yog suav hais tias yog ib qho ntawm cov txheej txheem tseem ceeb hauv lub zes qe menyuam thiab oviduct aging (30). Kev sim kev ntxhov siab hauv cov nas pom tau tias kev ntxhov siab ntev tuaj yeem ua rau MDA concentration thiab ua rau txo qis hauv kev tiv thaiv antioxidant hauv oviduct (33). Antioxidants muaj txiaj ntsig zoo hauv kev txo qis oxidative kev puas tsuaj rau oviduct. Astaxanthin muaj cov tshuaj tiv thaiv oxidative ntawm bovine oviduct epithelial hlwb vim induction ntawm antioxidant noob (34). Hauv txoj kev tshawb fawb tam sim no, qhov sib ntxiv ntawm LAM rau kev noj zaub mov ua rau nce cov tshuaj tiv thaiv antioxidant ntawm oviduct uterine ib feem thiab cov ntshav. Raws li peb cov txiaj ntsig, ntau qhov kev tshawb fawb tau qhia tias LA tuaj yeem txhim kho cov tshuaj tiv thaiv kab mob antioxidant thiab kev tiv thaiv kab mob hauv cov hens, txhim kho qe zoo, txhim kho nqaij zoo (13, 35), thiab txhim kho cov qib antioxidant hauv cov menyuam dev thiab nas (36, 37). Tsis tas li ntawd, raws li kev noj haus ntxiv, LA tau tshaj tawm los tiv thaiv oxidative stress-induced ntshav qab zib neuropathy thiab insulin tsis kam rau tib neeg (38). Hauv tib neeg, LAM tej zaum yuav muaj cov nyhuv antioxidant zoo dua rau qee yam kab mob.
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