Kev Tshawb Fawb Kev Tshawb Fawb Ntawm Phenylethyl Cawv GlycosidesinCistanche Ntawm Perimenopausal Syndrome Tsiaj Qauv Ⅱ

Kev sim 1 Kev Npaj Cistanche phenylethanoid glycoside 1 Qhov chaw ntawm cov khoom siv tshuaj

Cistanche tubulosa, Chengdu Wecistanche biotech Ltd.d, batch naj npawb: 20130501.

2 reagents

95% ethanol (qib kho mob); AB -8 macroporous adsorption resin, Tianjin Guangfu Fine Chemical Research Institute, batch tooj: 20130618.

Echinacoside siv tshuaj, muab los ntawm Tuam Tshoj lub koom haum rau Kev Tswj Xyuas Tshuaj thiab Khoom Siv Tshuaj lom neeg, batch tooj: 111670-200503.3

Nws yuav siv sij hawm ntev npaum li cas rau CISTANCHE ua haujlwm?

Txoj kev npaj

Kev siv tau ua rau txoj kev sau ntawv [19] thiab tau ua raws li kev qhia ntawm xibfwb Feng Suxiang los ntawm Lub Tsev Haujlwm Saib Xyuas Tshuaj Tshuaj ntawm Henan College of Traditional Chinese Medicine.Grind Cistancherau hauv cov hmoov ntxhib, ntxiv 10 zaug ntawm qhov nyiaj ntawm 70% ethanol rau reflux, thiab rho tawm ob zaug, 1.5 teev txhua lub sijhawm, muab ob qho dej cawv rho tawm, mloog zoo rau hauv qhov txo qis kom txog thaum tsis muaj cawv, thiab tawg. nrog dej distilled (qhov concentration yog 0.5g cov khoom siv tshuaj / ml) raws li cov qauv tshuaj. Dhau los ntawm AB-8 resin (kem tshuaj: resin yog 1:10), thauj cov qauv hauv qhov ntws, thiab cia nws sawv ntsug li 5 teev kom txog thaum nws tau nqus tag nrho. Ua ntej, ntxuav nrog 10 npaug ntawm sab ntim ntawm cov dej distilled, pov tseg cov dej, thiab tom qab ntawd siv 10 npaug ntawm sab ntim. Siv 10% ethanol tshem tawm impurities, thiab thaum kawg elute nrog 7 zaug ntawm kem ntim ntawm 60% ethanol. Sau cov eluate thiab evaporate rau dryness kom tau ib tug hmoov. 4 Kev ntsuas wavelength xaiv

Precisely ntsuas {{0}}.5 mL ntawm kev siv tshuaj, ntxiv 1 mL ntawm 5% sodium nitrite tov, co zoo, thiab cia sawv ntsug rau 6 feeb; ntxiv 1 mL ntawm 10% txhuas nitrate tov, co zoo, thiab cia sawv ntsug rau 6 feeb; Ntxiv 10% sodium hydroxide tov 10 mL, dilute mus rau 25 mL nrog dej, co zoo, thiab cia sawv ntsug rau 18 feeb los ua kev siv tshuaj. 0.5 ml ntawmCistanche phenylethanol glycosidetov tau npaj ib yam li cov tshuaj ntsuam xyuas. Siv cov qauv piv txwv tsis ntxiv cov ntaub ntawv siv thiab sim cov tshuaj raws li qhov khoob, thiab npaj cov tshuaj dawb huv tib yam. Ntawm UV spectrophotometer, ua ib qho kev soj ntsuam wavelength tag nrho ntawm peb qhov kev daws teeb meem saum toj no hauv qhov wavelength ntau ntawm 200-800nm. Ob qho khoom siv thiab cov tshuaj ntsuam xyuas muaj qhov siab tshaj plaws nqus ntawm 507nm, yog li 507nm tau txiav txim siab raws li qhov nqus nqus wavelength.

Tom qab xim kev loj hlob ntawm Cistanche phenylethanol glycoside qauv + xim kev loj hlob ntawm Echinacea tangerine siv tshuaj (superimposed sib piv), saib daim duab 1.

Daim duab 1 Tom qab xim kev loj hlob ntawm Cistanche phenylethanoid glycoside qauv + tom qab xim kev loj hlob ntawm Echinacea mandarin txiv kab ntxwv siv tshuaj Daim duab 5 Cov ntsiab lus nrhiav kom tau txoj kev

Precisely ntsuas 1 mL ntawm cov tshuaj yuav tsum tau kuaj, ntxiv 1 mL ntawm 5% sodium nitrite tov, co zoo, thiab cia sawv ntsug rau 6 min; ntxiv 1 mL ntawm 10% txhuas nitrate tov, co zoo, thiab cia sawv ntsug rau 6 min; ntxiv 10% sodium hydroxide Cov tshuaj yog 10 mL, dilute mus rau 25 mL nrog dej, co zoo, thiab cia sawv ntsug rau 18 feeb. Siv cov qauv piv txwv tsis ntxiv cov qauv kev daws teeb meem raws li qhov khoob, thiab npaj cov tshuaj dawb huv tib yam. Ntsuas siv UV spectrophotometer ntawm 507nm. 6 Methodological tshuaj xyuas

6.1 Kev npaj cov lus qhia siv thiab cov qauv kev daws teeb meem

Kev npaj cov tshuaj siv: Ua kom hnyav 3. co zoo, thiab tso tseg. Tus qauv concentration yog 0.1224 mg / ml.

Kev npaj cov qauv tshuaj: Precisely hnyav 5 mg ntawmCistanche phenylethanoid glycoside rho tawmrau hauv 10 ml volumetric hwj, yaj nws nrog 70% ethanol thiab dilute mus rau lub ntim.

6.2 Kev npaj cov qauv nkhaus

Yog kos {{0}}, 1.0, 2.0, 3.0, 4.{{10}}, 5.0 , 6.0 mL ntawm kev siv tshuaj, tsim cov xim thiab dilute rau ntim raws li txoj kev hauv 4.2, thiab ntsuas qhov absorbance tus nqi ntawm 507 nm. Siv cov concentration piv rau absorbance los ua ib qho kev nkhaus regression, qhov sib npaug regression yog: Y=20.296X-0.0015 (r=0.9994), thiab cov kab sib chaws yog 4.{{ 23}}.4 ug/ml.

6.3 Precision xeem

Siv cov qauv kev daws teeb meem thiab ntsuas nws rau lub sij hawm ua ke raws li "Tsev Kawm Ntawv Txiav Txim" yam khoom. Xam qhov txheeb ze tus qauv sib txawv ntawm qhov nqus. Cov absorbances yog {{0}}.299, 0.299, 0.298, 0.297, 0.297, 0.297 raws. Qhov nruab nrab absorbance=0.298, RSD=0.330% (n =6).

6.4 Stability test

Siv tus nqi tsim nyog ntawm cov qauv kev daws teeb meem, thiab raws li "Txoj Kev Txiav Txim Siab" cov khoom, ntsuas qhov ntsuas qhov nqus ntawm {{0}}, 15, 30, 50 , thiab 60 feeb tom qab qhov kev daws teeb meem yog xim tag, thiab xam tus txheeb ze tus qauv sib txawv. Cov absorbances yog 0.304, 0.303, 0.300, thiab 0.296 feem. , 0.295, 0.295, nruab nrab absorbance=0.299, RSD=1.362% (n=5).

6.5 Repeatability test

Ua ib qho qauv, npaj 6 cov qauv kev daws teeb meem raws li "Kev Npaj Cov Qauv Txheej Txheem" ​​cov khoom, thiab ntsuas cov nqi nqus raws li cov khoom "Kev txiav txim siab cov ntsiab lus", thiab suav cov ntsiab lus. Cov ntsiab lus yog 65.2%, 65.6%, 66.7%, 67.2%, thiab 66.5% feem. , 65.8%, nruab nrab cov ntsiab lus 66.2%, RSD=1.142% (n=6).

6.6 Sample recovery test

Precisely kos 0.5 mL ntawm cov qauv kev daws teeb meem nrog cov ntsiab lus paub, tag nrho ntawm 9 qhov chaw, thiab ntxiv 200, 250, thiab 300 ul ntawm cov qauv kev daws teeb meem feem. Ntsuas thiab xam tus nqi rov qab thiab txheeb ze tus qauv sib txawv ntawm Cistanche phenylethanol glycoside. Tus nqi rov qab yog 101.45% thiab 99.26% feem. , 98.94%, 101.83%, 99.05%, 99.47%, 98.72%, 99.17%, 100.64%, qhov nruab nrab rov qab yog 99.84%, RSD=1.164% (n=9).

7 Kev txiav txim siab cov ntsiab lus

Table 1 Kev txiav txim siab ntawm phenylethanoid glycoside cov ntsiab lus hauv Cistanche