Immunoregulation Thiab Anti-tumor Effects Of Polyporusus Bellatus: Kev Tshawb Fawb Txog Kev Tshawb Fawb Tsis ntev los no

Hu rau: emily.li@wecistanche.com

Miao-Miao Lin, Mei-Yu Cui1, Hai-Yan Cao, Xue-Song Wu, Li-Jing Cai, Li-Hui Zhang, Guo-Wei Zhang

1 Lub Tsev Kawm Qib Siab Suav Tshuaj, Hebei University, Baoding, Hebei xeev, Suav.

2 Laiyuan tsev kho mob ntawm Suav Tshuaj, Laiyuan, Hebei xeev, Suav.

Qhov tseem ceeb

Polyporus Bellatus (PPS) yog qhov tseem ceeb ntawm Zhuling (Polyporus). Qhov kev tshaj tawm no thawj zaug saib cov ntaub ntawv tseem ceeb ntawm PPS hauv xyoo tas los no. PPS muaj kev tiv thaiv kab mob, tiv thaiv daim siab, thiab tiv thaiv qog nqaij hlav. PPS tuaj yeem siv tshuaj tiv thaiv kab mob, hepatoprotective, thiabtshuaj tiv thaiv qogcuam ​​tshuam los ntawm ntau txoj hauv kev thiab ntau lub hom phiaj, uas muab kev pom zoo rau kev thov hauv tsev kho mob.

Cistanche tuaj yeem txhim kho kev tiv thaiv

Abstract

Peb tau piav qhia txog Polyporus Bellatus (PPS) kev ua tau zoo ntawm kev tiv thaiv kab mob, tiv thaiv daim siab, thiab tiv thaiv qog, tom qab ntawd muab cov hauv paus rau kev tshawb fawb ntxiv thiab kev siv tshuaj kho mob. Qhov kev tshaj tawm no thawj zaug saib cov ntaub ntawv tseem ceeb ntawm PPS nyob rau xyoo tas los no thiab piav qhia meej txog kev tshawb fawb txog kev tiv thaiv kab mob, tiv thaiv lub siab, thiab tiv thaiv qog nqaij hlav thiab cov txheej txheem ntawm PPS. Kev tshuaj xyuas qhia tau hais tias PPS ua si los tiv thaiv qog nqaij hlav los ntawm kev tiv thaiv oxidation, tshem tawm cov dawb radicals, inhibiting qog cell proliferation,inductionapoptosis, cuam tshuam cov qog gene nthuav qhia, thiabtxhim khotiv thaiv kab mobkev ua haujlwm. PPS tuaj yeem tiv thaiv kev tiv thaiv kab mob, hepatoprotective, thiab tiv thaiv qog nqaij hlav los ntawm ntau txoj hauv kev thiab ntau lub hom phiaj, uas muab kev pom zoo rau kev thov hauv tsev kho mob.

Ntsiab lus: Polyporusus Bellatus, Immunoregulation,Anti-mob, tshuaj xyuas

Cov ntawv luv: PPS, Polyporusus Bellatus; BMDCs: pob txha pob txha dendritic hlwb; TLR4: Tus xov tooj zoo li tus txais 4; IFN-: Interferon-; IL-1 : Interleukin-1 ; INOS: Inducible Nitric Oxide Synthase; TNF-: Tumor Necrosis Factor; TLR2: Tus xov tooj zoo li tus txais 2; LAK: lymphokine-activated killer; RIL-2: Recombinant Interleukin-2; NK: Ntuj Killer Cell; TSIS MUAJ: Nitric Oxide; GSH: Glutathione; BCG: Bacille Calmette-Guerin; PPL: PP-lymphocyte; NF-κB: Nuclear factor-kappa beta; CCl4: Carbon Tetrachloride; PCR: Polymerase Chain Reaction; GPx: Glutathione Peroxidase; CAT: Catalase; SOD: Superoxide Dismutase; PPAR-: Peroxisome Proliferator-activated Receptor Gamma.

Keeb kwm

ZhuLing (Polyporus) yog ib hom tshuaj suav tshuaj ntsuab uas tau siv dav hauv Suav teb rau ntau tshaj 2000 xyoo. Nws yog ib txwm siv los ua tus neeg sawv cev diuretic los kho cov kab mob urinary [1]. Nyob rau hauv xyoo tas los no, tus nqi tshuaj ntawm Zhuling tau txais kev saib xyuas ntau dua. Ntau tus kws tshawb fawb kho mob tau tshawb fawb ntau yam ntawm nws cov khoom xyaw nquag, cov tshuaj pharmacological, thiab cov txheej txheem. Polyporus Bellatus (PPS) yog qhov tseem ceeb ntawm Zhuling thiab muaj kev tiv thaiv qog thiab tiv thaiv kab mob. PPS muaj cov teebmeem tshwj xeeb hauv inhibiting qog loj hlob [2,3].

Polysaccharide muaj pes tsawg leeg yog paub hauv Zhuling

Zhuling muaj PPS. Tsib polysaccharides tam sim no cais tawm ntawm Zhuling, uas nws lub npe, hom, thiab muaj pes tsawg leeg ntawm monosaccharides feem yog: (1) Polyporus Bellatus, dej-soluble polysaccharide 6-branched beta (1→3)-Dextran [4]; (2) Dextran, tsim los ntawm condensation ntawm -(1→3), -(1→4) thiab (1→6) glucosidic bonds [5]; (3) Polyporusus Bellatus, muaj cov piam thaj thiab galactose [6]; (4) Polyporusus Bellatus (1 → 6) -D-glucopyranose skeleton, ceg los ntawm -D-Glcp, (1 → 3) DGlcp, (1 → 3) -D-GlcpA, (1 → 4) -D-Glcp thiab (1 → 4) -D-GlcpA muaj pes tsawg leeg [7]; (5) Polyporusus Bellatus (1 → 6,1 → 4) -D-glucopyranose skeleton muaj qhov hloov pauv ntawm (1 → 6) D-glucopyranose ceg los ntawm (1 → 3) -d glucopyranosyl [8].

Lub ntsiab pharmacological teebmeem ntawm PPS

PPS muaj cov nyhuv immunomodulatory

PPS yog bioactive polysaccharide. Li, XQ, et al. qhia tias PPS tuaj yeem ua rau muaj kev sib txawv thiab kev loj hlob ntawm murine pob txha pob txha dendritic hlwb (BMDCs). PPS txhawb nqa BMDCs ntau lawm ntawm Interleukin -12 (IL-12) p40 up-regulates cov kev qhia ntawm co-stimulatory molecules thiab txhim khu lub stimulation ntawm naïve T hlwb ntawm tus xov tooj zoo li Receptor 4 (TLR4) [9] . Jiang, Z. et al. xaus lus tias M1 macrophages tuaj yeem raug ntxias los ntawm RAW264.7 hlwb los ntawm kev txhawb nqa Interferon- (IFN-) ib leeg. Tsis tas li ntawd, PPS txhawb nqa mRNA kev qhia ntawm Interleukin-1 (IL-1), Inducible Nitric Oxide Synthase (INOS), Interleukin-10 (IL-10), Transforming Growth Factor- (TGF- ), thiab Tumor Necrosis Factor (TNF) hauv M1 macrophages [10]. Li, XQ. ua al. soj ntsuam phenotype thiab kev ua haujlwm ntawm PPS los ntawm 3H-TdR kev koom ua ke. Piv nrog rau cov pab pawg tsis zoo, lawv pom tias PPS tuaj yeem ua rau muaj kev sib koom ua ke ntawm CD11c thiab CD86 molecules ntawm Dendritic Cells (DC) nto thiab tsim cov IL-12 thiab IL{28}} nyob rau hauv ib tug npaum li cas ntawm koob tshuaj. PPS kuj tau txhim kho kom loj hlob BMDCs muaj peev xwm ntawm T cell pib ua haujlwm thiab txo phagocytosis ntawm BMDCs. Anti-TLR4, tab sis tsis yog anti-xws li Receptor 2 (TLR2) los yog ntxiv receptor 3 (CR3) monoclonal antibodies inhibited PPS-induced zus tau tej cov IL-12 p40 thiab thaiv kev sib xyaw ntawm fluorescence-labeled PPS (f- PPS) thiab BMDCs. Lawv cov ntaub ntawv qhia tau hais tias PPS tuaj yeem txhawb nqa kev ua kom murine BMDC ntawm TLR4 thiab kev loj hlob ntawm kev ua haujlwm immunomodulatory [11]. Li, JF, et al. tshawb xyuas cov kev ua ntawm PPS, mycobacterium polysaccharide (MPS), thiab lentinan (LEN) ntawm lymphokine-activated killer (LAK) cell kev ua hauv vitro hauv txoj kev tshawb no. Tib neeg peripheral ntshav mononuclear hlwb (PBMC) tau coj mus kuaj rau 96 teev nrog qhov nruab nrab uas muaj qhov sib txawv ntawm cov tshuaj uas tau hais los saum toj no ua ke nrog Recombinant Interleukin-2 (RIL-2). Tom qab ntawd cell-mediated lysis tau txiav txim siab los ntawm 1H-TdR tso tawm kev soj ntsuam nrog rau ntuj killer cell (NK) rhiab thiab ntuj killer cell resistant lub hom phiaj. Lawv xaus lus tias thaum ua ke nrog RIL-2 hauv ib qho kev xav, tag nrho peb hom polysaccharides tuaj yeem txhim kho LAK kev ua haujlwm los ntawm 42 feem pua ​​-56.9 feem pua ​​, thiab txo cov koob tshuaj RIL-2 los ntawm 50 feem pua. Nws tau hais tias PPS, mycobacterium polysaccharide, thiab lentinan tuaj yeem siv los ua bioactivity regulators hauv LAK cell kho hauv kev kho mob qog nqaij hlav [12]. Nie Hong, et al. tau lees paub tias ib qho Rprescription ntawm PPS tau pom tias muaj peev xwm rov qab los ntawm lymphocyte transformation muaj peev xwm, tua cov haujlwm ntawm NK hlwb, tag nrho cov T cells, thiab IgG antibody muaj peev xwm tsim tau hauv nas nrog cyclophosphamide [13]. Zhang Juncai, et al. tau lees paub tias PPS tuaj yeem txhim kho kev lom zem thiab kev tiv thaiv kab mob ntawm tes hauv Cavia porcellus, thiab PPS yog lub zog tiv thaiv kab mob [14]. Hou Gan, et al. pom tias PPS tuaj yeem nce INOS kev ua haujlwm hauv nas peritoneal macrophages, txhawb nqa Nitric Oxide (NO) synthesis, thiab haus intracellular Glutathione (GSH). Nws qhia tias intracellular GSH tuaj yeem ua lub luag haujlwm hauv kev tswj tsis muaj kev tsim tawm hauv macrophages thiab tiv thaiv cov tswv tsev ntawm NO-mediated cytotoxicity [15]. Zhang, et al. tau qhia hauv vivo cov kev tshawb fawb tias kev sib koom ua ke ntawm PPS ua ke nrog Bacille Calmette-Guerin (BCG) tuaj yeem ua rau muaj txiaj ntsig zoo rau kev qhia ntawm co-stimulatory molecules CD86, CD40, thiab TLR4/CD14. Immunohistochemical tsom xam pom tias CD86 thiab CD40 staining tau tshaj tawm [16]. Jiang Zb, et al. tau kawm txog cov txiaj ntsig ntawm PPS ntawm kev tswj hwm ntawm lipopolysaccharide (LPS)-vim cov cytokines hauv J774 macrophage o qauv thiab kawm txog nws cov txheej txheem tiv thaiv kab mob uas yuav yog los ntawm mitogen-activated protein kinase (MAPK) qhia txoj hauv kev kom txo tau kev puas tsuaj [17 ]. Jiang ZB et al. tau soj ntsuam cov txiaj ntsig ntawm PPS ntawm INF- inducing M1 subtype macrophage membrane nto protein thiab nws cov teebmeem ntawm cov cytokines IL-1 , Interleukin -6 (IL-6), thiab TNF- mRNA. Hauv hom macrophage M1, nce kev qhia ntawm M1 inflammatory cytokines ntxias los ntawm INF-y thiab cov tshuaj tiv thaiv kab mob cytokine tib lub sijhawm yuav muaj kev tswj hwm kev tiv thaiv kab mob bidirectional [18]. Xu, et al. pom tias PPS tuaj yeem txhawb kev loj hlob ntawm cov nas spleen hlwb thiab tsim cov NO, IL-1 , TNF- , thiab TLR4 hauv peritoneal macrophages [19]. Nws tau kwv yees tias PPS tuaj yeem ua rau nas peritoneal macrophages los ntawm TLR4 thiab ua lub luag haujlwm hauv kev tiv thaiv kab mob. Sun, et al. pom tias PPS tuaj yeem nce tus naj npawb ntawm NK thiab LAK hauv tus po ntawm nas thiab txhawb kev loj hlob thiab kev sib txawv ntawm lymphocytes B thiab lymphocytes T hauv nas [20]. Dai, et al. pom tias PPS tuaj yeem ua kom zoo B hlwb, macrophages, thiab cov hlwb zoo li tsob ntoo [21]. Cov polysaccharide epitopes muaj feem xyuam rau ib puag ncig antigens uas tuaj yeem ua rau kev hloov pauv lub cev tiv thaiv kab mob. PPS yog qhov tseem ceeb hauv kev tiv thaiv kab mob ntawm Zhuling. Pan WL, et al. pom tias PPS muaj qhov ua kom pom tseeb ntawm cov hlab ntsha hauv cov ntshav hematopoietic qia hlwb [22]. Nws tuaj yeem txhawb kev hloov pauv ntawm nas lub cev tiv thaiv kab mob hematopoietic rov tsim kho ntawm cov hlab ntshav hematopoietic qia hlwb. Hauv kev muaj sia nyob, ntshav rov qab los, thiab cov ntsiab lus ntawm lub sijhawm thiab cov txiaj ntsig, pawg PPS hloov pauv tau zoo dua rau pawg nas hloov pauv, thiab muaj qhov sib txawv tseem ceeb hauv qib CD3 ntxiv, CD4 ntxiv, CD8 ntxiv, thiab CD19 ntxiv. Zhang, et al. siv cov kev txhaj tshuaj ntawm hom II collagen los tsim ib tus nas qauv ntawm kev mob caj dab (CIA) thiab cais nas PP-lymphocyte (PPL), plab hnyuv epithelial lymphocytes, thiab lamina propria lymphocytes, co-cultured nrog ntau ntau ntawm PPS [23]. Cov txiaj ntsig tau pom tias PPS muaj qhov sib txawv ntawm cov tshuaj tiv thaiv kab mob ntawm cov nas PPL, uas tuaj yeem txo qhov tso tawm ntawm TNF- los ntawm PPL thiab nce qhov tso tawm ntawm IFN-. Cov theem ntawm TNF- thiab IFN- secreted los ntawm plab hnyuv Epithelial Lymphocytes thiab Lamina Propria Lymphocytes raug txo kom ntau qib. Li X, et al. pom tias PPS tuaj yeem txhim kho kev ua haujlwm ntawm macrophages, xws li kev tsim cov NO thiab kev qhia ntawm cytokines. PPS tau txhawb nqa kev loj hlob ntawm splenocytes thiab tsim cov TNF- , IL-1 , thiab TSIS TAU ntawm peritoneal macrophages los ntawm C3H / HeN nas. Cov tshuaj tiv thaiv kev ua haujlwm rau TLR4, tab sis tsis yog TLR2 thiab CR3, tau txwv tsis pub PPS-mediated TNF- thiab IL-1 ntau lawm. Nuclear translocation thiab DNA binding kev ua haujlwm ntawm nuclear kappa beta (NF-κB) tau tshwm sim los ntawm PPS. Lawv cov ntaub ntawv qhia tias PPS tuaj yeem ua rau nws cov tshuaj tiv thaiv kab mob los ntawm TLR{120}} ua kom muaj kev taw qhia [24].

Kev tiv thaiv daim siab

PPS tuaj yeem txo qhov kev puas tsuaj ntawm carbon tetrachloride rau nas lub siab, txiavdaim siabkab mobkev puas tsuaj, txo cov ntshav alanine aminotransferase kev ua haujlwm, tiv thaiv lub siab 6 phosphoglucomutase thiab khi cov kua qaub phosphatase los ntawm kev txo qis [25]. Huang DN, et al. pom tau hais tias kev tiv thaiv kev ua haujlwm ntawm PPS tau siv Carbon Tetrachloride (CCl4)-induced hepatic raug mob hauv tus qauv nas. Cov teebmeem ntawm PPS tau raug soj ntsuam los ntawm biochemical qhov tseem ceeb thiab kev kuaj histopathological; mRNA qhia tau ntsuas los ntawm lub sijhawm tiag tiag Polymerase Chain Reaction (PCR). Lawv pom PPS koob tshuaj-dependently alleviated hepatic raug mob tshwm sim los ntawm kev rov qab los ntawm Lactate Dehydrogenase (LDH), Aspartate Transaminase (AST), Alanine Transaminase (ALT), Malondialdehyde (MDA), txo GSH, Glutathione Peroxidase (GPx), Catalase (CAT) , thiab Superoxide Dismutase (SOD) qib. Kev kuaj histopathological kuj tau lees paub qhov txo qis ntawm kev raug mob siab. Lub caij no, qhov kev tawm tsam mRNA ntawm GPx, CAT, thiab SOD los ntawm CCl4 tau rov qab los ntawm kev kho PPS. Cov ntaub ntawv no tau qhia tias PPS tiv thaiv CCl4-vim raug mob hepatic, thiab cov txheej txheem cuam tshuam nrog kev tswj hwm ntawm GPx, CAT, thiab SOD qhia [26]. Liu XL, et al. tau tsim ib qho qauv ntawm cov kua dej cawv hauv cov nas thiab tau kawm tias PPS tuaj yeem txo taudaim siabcellmobnyob rau hauv dej cawv fatty daim siab nas, paub meej tias PPS tuaj yeem kho daim siab puas tsuaj rau qee qhov [27]. Tau J, et al. pom tias qhov zoo ntawm cov ntshav HBsAb hauv cov neeg mob nrog PPS ua ke nrog cov tshuaj tiv thaiv kab mob siab B yog siab dua li cov ntshav HBsAb hauv cov neeg mob uas tau txhaj tshuaj tiv thaiv kab mob siab B ib leeg, thiab tus nqi ntawm tus kab mob siab B tau qis dua, uas qhia tau tias PPS yog tus Lub ntsiab active ingredient nyob rau hauv txo cov ntshav rog thiab lub siab tiv thaiv piglet [28]. Du JL, et al. pom tias PPS tuaj yeem cuam tshuam kev raug mob hepatocyte los ntawm CCl4, txo qis kev ua haujlwm ntawm Alanine Transaminase, Aspartate Transaminase, thiab Malondialdehyde hauv hepatocytes thiab nce tus nqi ciaj sia ntawm hepatocytes. Nyob rau tib lub sijhawm, CYP3A mRNA qhia tau cuam tshuam los ntawm PPS, uas muaj cov txiaj ntsig ntawm kev tiv thaiv hepatocytes [29]. Liu XL et al. tsim ib tus nas cawv uas muaj roj fatty siab tus qauv thiab teem ib koob me me (0.3 g/kg), koob tshuaj nruab nrab (1g/kg), koob tshuaj siab (3 g/kg) PPS pawg [30]. Cov txiaj ntsig tau pom tias qib ntawm cov roj (cholesterol) (TC) thiab Triglyceride (TG) hauv cov ntshav ntawm cov nas rog rog hauv txhua pab pawg tau txo qis, thiab qib ntawm cov kab mob siab cell steatosis tau txo. PPS muaj cov txiaj ntsig ntawm kev kho dej cawv fatty siab hauv nas. Cov nyhuv curative ntawm PPS ntawm cov kab mob siab B mob ntev yog qhov tseeb. Txawm hais tias nws yog siv ib leeg lossis ua ke nrog lwm cov tshuaj suav tshuaj, tshuaj tiv thaiv kab mob siab B, interferon, lamivudine, thiab al., nws tuaj yeem cuam tshuam qhov kev rov ua dua ntawm tus kab mob siab B thiab nce HBeAg thiab HBV-DNA qhov kev hloov pauv tsis zoo thiab tiv thaiv HBe zoo. tus nqi los txhim kho daim siab ua haujlwm. Tshwj xeeb tshaj yog nyob rau hauv kev sib xyaw nrog lwm cov tshuaj, nws tuaj yeem ua lub luag haujlwm kho mob hauv ntau yam, xws li kev txhim kho kev ua tau zoo, thiab qee qhov txo qis qhov tsis zoo ntawm lwm cov tshuaj, uas tau qhib txoj hauv kev tshiab rau kev sib xyaw ntawm Suav Tshuaj thiab Western Tshuaj kho mob. kab mob siab B mob ntev [31].

Anti-tumor nyhuv

Cov qog nqaij hlav zais zis yog ib qho ntawm cov qog qog nqaij hlav tshaj plaws uas cuam tshuam nrog macrophages. PPS tau pom tias muaj txiaj ntsig zoo hauv kev kho mob qog noj ntshav nrog cov kev mob tshwm sim tsawg. PPS tau hais tias yuav muaj txiaj ntsig zoo hauv inhibiting zais zis carcinogenesis hauv nas [32]. Tsis tas li ntawd, Zhuling tau ua pov thawj pom tias muaj txiaj ntsig zoo rau lub zais zis [33]. Peroxisome Proliferator-activated Receptor Gamma (PPAR-) yog ib tug tswv cuab ntawm lub nuclear receptor superfamily ntawm ligand-activated transcription yam uas tau txuam nrog inhibition ntawm qog cell loj hlob. Li CX, et al. soj ntsuam kev qhia ntawm PPAR- hauv tib neeg T24 zais zis qog nqaij hlav los ntawm immunocytochemistry, Real-time PCR, thiab Western blot. Lawv xaus lus tias PPS inhibited kev loj hlob ntawm zais zis qog nqaij hlav T24 hlwb. PPAR-antagonist bisphenol A diglycidyl ether (BADGE) thim rov qab PPS xav txog kev loj hlob ntawm tes inhibition [34]. Cov kev tshawb fawb tau lees paub tias PPS inhibits tib neeg lub zais zis kab mob qog noj ntshav T24 hlwb, inhibits kev loj hlob ntawm cov hlwb, thiab tiv thaiv S-phase hlwb los ntawm kev nkag mus rau theem G2 / M. PPS inhibits cell proliferation los ntawm kev cuam tshuam thiab cuam tshuam rau T24 cell voj voog. Nyob rau tib lub sijhawm, PPS tseem tuaj yeem ua rau T24 cell morphology hloov, qeeb ntawm cell proliferation, ua rau txo qis ntawm cov hlwb adherent thiab nce cov hlwb tuag [35]. Shen G, et al. kho cov pab pawg tswj T24 hlwb ib txwm, thiab cov pab pawg neeg sim tau incubated nrog ntau koob tshuaj PPS. Piv nrog rau pawg tswj hwm, lawv pom tias cell apoptosis tau nce, BCL-2 mRNA stability thiab BCL-2 mRNA thiab protein qhia tau txo qis hauv cov pab pawg nruab nrab thiab cov koob tshuaj PPS, tag nrho Cov protein ntawm Hu R tau hloov me ntsis, tab sis cov cytoplasm Hu R protein tau qis-tswj thiab cov nucleus Hu R protein tau hloov kho. Lawv txiav txim siab tias PPS tuaj yeem ua rau apoptosis ntawm T24 hlwb, uas muaj feem cuam tshuam rau kev txo qis ntawm cytoplasm Hu R protein thiab nrog kev txo qis ntawm BCL 2 m RNA stability thiab BCL -2 protein qhia [36]. Liu CP, et al. siv macrophages kab lis kev cai ib leeg lossis nrog T24 tib neeg lub zais zis mob qog noj ntshav cell kab lis kev cai supernatant raws li kev kawm qauv. Lawv pom tias PPS txhim kho cov dej num ntawm IFN stimulated RAW 264.7 macrophages, raws li qhia los ntawm kev tso tawm INOS, tso tawm ntawm TNF thiab Interleukin -6 (IL-6), phagocytosis kev ua, nrog rau cov kab lus ntawm M1. phenotype indicators, xws li CD40, CD284, thiab CD86. PPS tau ua raws li cov dej ntws hauv qhov ua kom muaj zog ntawm cov khoom siv hluav taws xob nuclear NF-κB qhia txoj hauv kev los ntawm kev cuam tshuam nrog NF-κB phosphorylation. Tsis tas li ntawd, PPS tswj hwm NF-κB P65 teeb liab los ntawm kev cuam tshuam nrog Tus Xov Tooj Zoo li TLR-4, INOS, thiab cyclooxygenase COX-2. Lawv cov txiaj ntsig tau qhia tias PPS qhib cov macrophages los ntawm TLR4 / NF-κB txoj kev taw qhia [37].

Covtiv thaiv-qogCov txiaj ntsig ntawm PPS tau pom tias muaj feem cuam tshuam rau nws qhov kev txhawb nqa ntawm TSIS tso tawm los ntawm macrophages, thiab tib lub sijhawm stimulation ntawm macrophages nrog rBCG-IL-2 thiab PPS yog qhov muaj zog tshaj qhov uas txhawb nqa nrog rBCG-IL-2 ib leeg [ 38] ib. Huang, DN et al. tau soj ntsuam qhov tsim ntawm NO, qhov kev ua, thiab mRNA qhia ntawm INOS nyob rau hauv peritoneal macrophages ntawm nas siv nrog ntau koob tshuaj PPS los ntawm Griess cov tshuaj tiv thaiv, fluorimetry assay, thiab RT-PCR, raws li. Lawv pom tias PPS tuaj yeem txhawb nqa iNOS kev ua haujlwm nrog kev siv tshuaj noj thiab txhawb nqa iNOS mRNA kev qhia ntawm peritoneal macrophages hauv nas. Raws li qhov tshwm sim, lawv xav tias txoj cai ntawm PPS ntawm kev tsim cov NO hauv peritoneal macrophages ntawm nas yuav tshwm sim ntawm qib transcriptional ntawm iNOS. Qhov no qhia tau hais tias cov txheej txheem ntawm PPS's antitumor thiab immunoregulation functions tej zaum yuav muaj feem xyuam rau nce NO tso zis ntawm macrophages los ntawm stimulating iNOS's denovo synthesis [39].

Nws tau pom tias PPS tuaj yeem txhawb nqa qhov kev qhia ntawm CD14, TLR2, thiab TLR4 molecules thiab kev qhia ntawm adhesion molecule CD11b nyob rau saum npoo ntawm macrophages nyob rau theem pib ntawm BCG synergy. Yog li ntawd, TLR cov molecules thiab cov kev taw qhia yuav raug siv los ua ib txoj hauv kev tseem ceeb los kho PPS thiab BCG-induced kab mob tiv thaiv kab mob thiab induce anti-tumor effect [40]. Nws kuj tau pom tias rBCG tuaj yeem nkag ncaj qha mus rau cytoplasm ntawm T24 hlwb, thaum PPS muaj txiaj ntsig los txhawb kev hloov pauv ntawm rBCG los ntawm cytoplasm mus rau lub nucleus ntawm cov qog hlwb [41]. Tom qab T739 hlwb raug kho nrog PPS, BCG thiab lawv ua ke, cov kev hloov hauv mRNA thiab protein qhia ntawm inhibitor ntawm kappa B kinase beta (IKK), NF-κB subunit p65 (NF-κB p65), ICAM1, thiab CCL2 nyob rau hauv lub zais zis cancer. Cov kab ntawm tes T739 tau txiav txim siab los ntawm cov txheeb ze txheeb ze ntawm PCR, Western blot thiab ntws cytometry. NF-κB p65 DNA-binding kev ua si hauv T739 hlwb tau kuaj pom los ntawm biotinylated sojntsuam-ELISA, thiab NF-κB p65 nuclear qhia hauv T739 hlwb tau soj ntsuam los ntawm immunohistochemistry. Wei JA et al. xaus lus tias PPS tuaj yeem cuam tshuam qhov kev ua kom dhau ntawm NF-κB kev taw qhia txoj hauv kev tshwm sim los ntawm BCG hauv cov qog nqaij hlav zais zis thiab ua kom txo qis qhov tsis zoo rau kev kho BCG [42]. Hauv kev tshawb fawb, Zhang GW et al. pom tias kev txhim kho qog nqaij hlav hauv lub zais zis hauv cov qauv nas pom tau txo qis kev mob qog noj ntshav nrog Zhu Ling PPS ua ke nrog BCG. Flow cytometric tsom pom kev qhia ntawm costimulatory molecules CD86, CD40, thiab TLR4/CD14 nce ntxiv nrog Zhu Ling PPS ua ke nrog BCG. Lawv qhov kev tshawb pom tau pom tias Zhu Ling PPS txo qis cov kev mob tshwm sim thiab pom muaj kev sib koom ua ke thaum BCG instillation hauv nas zais zis qauv [16]. Nws tau pom tias PPS ua ke nrog BCG tuaj yeem txhim kho kev qhia ntawm CD11b, CD18, thiab costimulatory molecules [43]. Zhang et al. pom tias PPS ua ke nrog BCG tuaj yeem tiv thaiv qog nqaij hlav qog nqaij hlav hauv nas thiab txo cov kev mob tshwm sim ntawm BCG hauv lub cev [16]. Nyob rau tib lub sijhawm, kev qhia ntawm CD86, CD40, thiab TLR4/CD14 tau nce hauv nas peritoneal macrophages thiab zais zis epithelial hlwb. CD86 ntawm cov qog nqaij hlav qog nqaij hlav epithelial tsis tau qhia los ntawm cov qog nqaij hlav cancer. Yang Dao, et al. siv cov carcinogen OH-BBN los ua tus qauv qog nqaij hlav zais zis hauv cov poj niam nas thiab pub 90 g / kg qhuav hmoov ntawm swine fever. Tom qab 30 lub lis piam, cov nas tau txi. Lawv tau soj ntsuam cov teebmeem ntawm Zhuling rau cov nas mob qog noj ntshav. Cov txiaj ntsig tau pom tias tag nrho cov qog ntawm lub zais zis txo los ntawm 38.9 feem pua ​​​​piv rau pawg tswj kab mob. Cov qog txoj kab uas hla ntawm ib tus nas tau qis dua li pawg tswj hwm pathological. Tus mob qog noj ntshav poob qis los ntawm 66.7 feem pua ​​​​piv rau pawg tswj kab mob. Nws tau pom tias Zhuling muaj qhov cuam tshuam loj heev rau qhov tshwm sim ntawm OH-BBN zais zis mob cancer, thiab tsis muaj qhov pom tseeb tshwm sim [44]. Cui C, thiab al.studied nyob rau hauv vitro lub polysaccharide secreted los ntawm PPS mus suppress lub secretion ntawm immunosuppressed molecules nyob rau hauv cov qog hlwb thiab pom tias PPS muaj ib tug tiv thaiv nyhuv ntawm cov qog nyob rau hauv mob cancer Colon26 hlwb [45]. Li CX, et al. siv BBN thiab saccharin dej los txhawb kev tsim ntawm Fisher344 nas zais zis qauv [46]. Cov kev tshawb fawb tau pom tias Zhuling thiab PPS tuaj yeem cuam tshuam qhov tshwm sim thiab kev loj hlob ntawm cov qog los ntawm kev cuam tshuam rau thymus, spleen Performance index, thiab lymphocyte infiltration, thiab CD86 qhia hauv zais zis cov ntaub so ntswg thiab precancerous cov ntaub so ntswg ntawm lub zais zis qauv nas. Qin GF, et al. qhia tau tias Zhuling muaj qhov cuam tshuam loj heev rau cov qauv qog nqaij hlav zais zis uas tshwm sim los ntawm N-butyl-N-4-hydroxy butyl nitrosamine (BBN) hauv cov qauv nas ntawm lub zais zis. [47] Qhov kev qhia ntawm Aquaporin 1 (AQP1) thiab Aquaporin 3 (AQP3) protein qhia tau hais tias hydrophobization kev ua tau zoo ntawm swine fever tuaj yeem cuam tshuam kev loj hlob ntawm zais zis los ntawm inhibiting kev qhia ntawm AQP1 thiab AQP3. Jiang ZB, et al. siv macrophage RAW264.7 ua ib vector los induce qhov mob ntawm M1 subtype macrophages los ntawm IFN- [48]. Lawv tau kawm txog cov nyhuv ntawm PPS ntawm M1 daim nyias nyias ntawm cov protein qhia thiab nws cov cytokines cuam tshuam thiab tshawb xyuas cov txheej txheem inhibitory ntawm PPS ntawm cov qog. Cov txiaj ntsig tau pom tias PPS muaj kev tswj hwm ob txoj kev tswj hwm thiab tuaj yeem polarize macrophages rau hom M1 thiab nce qhov kev qhia ntawm M1 inflammatory factor. Zeng, et al. tau pom tias PPS feem ntau inhibits TLR4 teeb liab txoj hauv kev los ntawm inhibiting kev qhia ntawm cov noob muaj feem xyuam, NF-κB p65 DNA txoj kev sib khi ua haujlwm, thiab cov lus qhia nuclear los inhibit qog kev loj hlob [49]. Cov kev tshawb fawb tau lees paub tias cov khoom siv hauv Zhuling inhibits kev loj hlob ntawm tib neeg cov qog hlwb HT-29 (mob qog noj ntshav), HeLa 229 (mob qog noj ntshav), Hep3B (mob qog noj ntshav), thiab AGS (mob qog noj ntshav), thiab nthuav tawm cytotoxicity. [50].

Xaus

Raws li kev siv tshuaj fungus feem ntau, PPS yog muab rho tawm los ntawm Zhuling. Nyob rau hauv xyoo tas los no, los ntawm kev tshawb fawb pharmacological niaj hnub no, nws tau lees paub tias muaj ntau yam kev ua ub no thiab cov tshuaj pharmacological. Nws ua rau muaj txiaj ntsig zoo rau kev siv tshuaj kho kab mob siab B thiab ua lub luag haujlwm tseem ceeb hauvtiv thaiv-qogmob qog noj ntshav. Los ntawm ntau yam kev tshawb fawb thiab kev tshawb fawb pharmacological, nws muab lub hauv paus theoretical rau nws daim ntawv thov kev kho mob thiab muaj kev taw qhia tseem ceeb.

Kev lees paub

Cov kws sau ntawv xav ua tsaug rau Guowei Zhang (MD thiab Ph.D., Tuam Tshoj) rau nws qhov kev pabcuam tseem ceeb.

Cov ntaub ntawv

1 National Pharmocopoeia Commission. Pharmacopoeia ntawm Cov Neeg Koom pheej ntawm Tuam Tshoj. Beijing: Beijing Chemical Industry Xovxwm, 2005: 222.

2. Hatanaka K, Satomi N, Sakurai A, thiab al. Antitumor kev ua ub no thiab qog necrosis qhov ua tau zoo ntawm cov tshuaj suav tshuaj thiab cov tshuaj tsis zoo. J Trad Chin Med 1985, 20: 1-5.

3. Han J. Cov tshuaj suav tshuaj thiab kev tshawb nrhiav cov tshuaj antineoplastic tshiab. J Ethnopharmacol 1988, 24: 1-17.

4. Wang YJ. Tshuaj tiv thaiv kab mob siab B tshiab-Polyporusus Bellatus txhaj. Chin J Hosp Pharm 1992, 12:477.

5. Feng QR, Fu GL, Meng B, et al. Kev txiav txim siab ntawm qhov hnyav molecular thiab viscosity ntawm Polyporusus Bellatus polysaccharides. Tuam Tshoj J Chin Mater Med 1987,12: 38-40.

6. Sun Y, Zhou X. Purification, thawj tus cwj pwm thiab kev tiv thaiv kab mob ntawm polysaccharides los ntawm fungus, Polyporus umbellatus. Food Sci Hum Well 2014, 3: 73-78.

7. He PF, Zhang AQ, Wang XL, et al. Cov qauv elucidation thiab antioxidant kev ua haujlwm ntawm ib qho tshiab polysaccharide los ntawm Polyporus umbellatus, sclerotia [J]. Int J Biol Macromol 2016, 82: 411-417.

8. Dai H, Han XQ, Gong FY, et al. Tus qauv elucidation thiab immunological muaj nuj nqi tsom xam ntawm ib tug tshiab -glucan los ntawm cov txiv hmab txiv ntoo lub cev ntawm Polyporus umbellatus (Pers.) Fries. Glycobiology 2012, 22: 1673-1683.

9. Li XQ, Wen X, Chen J. Polysaccharide purified los ntawm Polyporus umbellatus (Per) Fr induces lub activation thiab maturation ntawm murine pob txha-derived dendritic hlwb ntawm tus xov tooj hu-zoo li receptor 4. Cell Immunol 2010, 265: 50-56 .

10. Jiang Z, Huang R, Zhang X, et al. Kev tswj cov teebmeem ntawm Polyporus polysaccharide ntawm cov lus qhia ntawm cytokines tso tawm los ntawm M1 macrophages [J]. Chin J Cell Mol Immunol 2014, 30: 1030-1033.

11. Li, XQ, Xu W. Effect of Polyporus umbellatus polysaccharides on activation of murine bone marrow dendritic cells via Toll-like receptor 4. Chin Trad Her Drugs 2011, 42: 118-123.

12. Li JF, Guo JW, Huang XF. Kawm txog kev txhim kho cov nyhuv ntawm Polyporus polysaccharide, mycobacterium polysaccharide, thiab lentinan ntawm lymphokine-activated killer cell kev ua hauv vitro. Chin J Integr Med 1996, 16: 224-226.

13. Nie H, MA AL, Shen BH, et al. Immunoregulation nyhuv ntawm compound Polyporus umbellatus polysaccharide rau nas. J Cell Mol Immunol 2000, 16: 384-386

14. Zhou J.C. Kev cuam tshuam ntawm kev sib txawv adjuvants ntawm kev tiv thaiv ntawm BCG. Prog Vet Med 2003, 24: 96-98.

15. Chen WS, Hou G, Zhang HT. Cov teebmeem ntawm Polyporus polysaccharide ntawm TSIS MUAJ ntau lawm, iNOS kev ua, thiab intracellular txo glutathione qib los ntawm peritoneal macrophages ntawm nas. J Guangdong Med Coll 2003, 21: 319-320.

16. Zhang GW, Qin GF, Han B, et al. Kev ua tau zoo ntawm Zhuling Polyporus polysaccharide nrog BCG los tiv thaiv zais zis carcinoma. Carbohydrate Polym 2015,118: 30-35.

17. Jiang ZB, Li SM, Zhao J, et al. Anti-inflammatory Effect thiab Mechanism ntawm Polyporus Polysaccharide ntawm LPS-stimulated J774 Cells. Chin J Exp Trad Med Formul 2015, 21: 156-159.

...