Ntu Ⅱ: Cistanche: Ua kom muaj txiaj ntsig zoo ntawm Phenylethanoid Glycosides Ntawm Mesoporous Carbon
Mar 04, 2022
Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com
Helin Xu, Wenjing Pei, Xueqin Li, thiab Jinli Zhang
Kev sib piv ntawm Peb Mesoporous Carbons Adsorption Performance
Vim tias peb qhov adsorbents feem ntau yog tsim los ntawm cov pa roj carbon, qhov adsorption kev sib cuam tshuam ntawm PhGs thiab cov pa roj carbon mesoporous tau suav tias yog tib yam. Yog li, nws tau txiav txim siab tias qhov sib txawv ntawm kev ua haujlwm adsorption tau muab los ntawm qhov sib txawv ntawm lub cev qauv ntawm mesoporous carbons. Table 2 qhia txog kev ua haujlwm adsorption ntawm peb hom mesoporous carbons. Nws tuaj yeem pom los ntawm Table 2 tias peb hom mesoporous carbons txhua tus tuaj yeem nqus tau PhGs, thiab CMK-3 muaj qhov ua haujlwm zoo dua li CMK-8 thiab DMC. Qhov no qhia tias qhov pore loj ntawm mesoporous carbons loj dua PhGs molecules, thiab qhov pore loj tsis yog qhov tseem ceeb cuam tshuam rau lub peev xwm adsorption rau peb cov pa roj carbon ntau. Lub peev xwm adsorption thiab desorption tus nqi ntawm CMK-3 tau mus txog 189.37 mg / g thiab 94.96 feem pua, raws li. Lub peev xwm adsorption ntawm CMK-3 tau siab dua li ntawm CMK-8. Vim tias qhov pore ntim thiab thaj chaw tshwj xeeb ntawm CMK-3 loj dua DMC thiab CMK-8, nws tau qhia tias qhov pore ntim thiab thaj chaw tshwj xeeb yog qhov tseem ceeb cuam tshuam rau kev ua haujlwm adsorption.
Optimization ntawm adsorption tej yam kev mob
Kev ua kom zoo tshaj plaws ntawm kev sim adsorption rau CMK-3 tau ua tiav los ntawm kev sib xyaw ntawm ob qho tib si acteoside thiab echinacoside. Kub, pH, thiab concentration yog lub ntsiab tseem ceeb ntawm CMK-3 adsorption kev ua tau zoo. Yog li, cov teebmeem ntawm peb qhov cuam tshuam rau CMK-3 adsorption kev ua tau zoo raug tshawb xyuas.
Cov txiaj ntsig ntawm Cov Qauv Cuam Tshuam rau Adsorption Performance ntawm CMK-3
Cov nyhuv ntawm cov qauv concentration ntawm cov adsorption kev ua tau zoo ntawm CMK {{0}} tau pom nyob rau hauv daim duab 5. Raws li cov concentration nce, lub peev xwm ntawm adsorption nce. Lub peev xwm adsorption tsis nce ntxiv nrog qhov nce ntawm cov qauv concentration thaum cov qauv concentration tau mus txog ntawm 0.41 mg / g. Ntawm qhov qis qis pib ntawm cov qauv, cov chaw ua haujlwm adsorbent tau txaus rau adsorption ntawm ib qho me me ntawm PhGs molecules. Nyob rau hauv sib piv, nyob rau hauv ib tug siab pib concentration ntawm cov qauv, tus nqi ruaj khov ntawm cov chaw nquag ntawm cov adsorbents tsis muaj peev xwm adsorption nce tus nqi ntawm PhGs molecules. Yog li, lub peev xwm adsorption ntawm CMK-3 rau PhGs molecules zoo li sib npaug.
Qhov cuam tshuam ntawm pH ntawm Adsorption Performance ntawm CMK-3
Daim duab 6 tau pom qhov cuam tshuam ntawm pH ntawm kev ua haujlwm adsorption ntawm CMK-3. Nws tuaj yeem pom los ntawm daim duab 6 tias qhov zoo tshaj pH tus nqi yog 6 rau qhov adsorption ntawm PhGs los ntawm CMK-3. Cov laj thawj yog raws li hauv qab no: PhGs muaj ntau pawg phenolic hydroxyl thiab koom nrog cov tsis muaj zog acidic molecules, cov pH sib txawv cuam tshuam rau ionization thiab stability ntawm PhGs molecules. Lub ionization ntawm phenolic hydroxyl pawg yuav raug inhibited ntawm tus nqi pH qis, thaum lub zog ntawm phenolic hydroxyl pawg ntawm PhGs yuav txo qis ntawm tus nqi pH siab. Qhov inhibited ionization ntawm phenolic hydroxyl pawg ntawm PhGs ua rau txo qis hauv electrostatic kev sib cuam tshuam ntawm PhGs thiab CMK-3, txo qhov adsorption kev ua tau zoo ntawm CMK-3 rau PhGs. Yog li, qhov zoo tshaj pH yog 6 rau CMK- 3 adsorption.
Qhov cuam tshuam ntawm qhov kub thiab txias ntawm Adsorption Performance ntawm CMK-3
Qhov ntsuas kub yog qhov tseem ceeb hauv cov txheej txheem adsorption. Qhov kub tsis tsuas yog cuam tshuam rau qhov sib txawv ntawm PhGs molecules ntawm sab nraud ciam teb interface tab sis kuj nyob rau hauv cov adsorbent pores. Daim duab 7 tau pom qhov cuam tshuam ntawm qhov kub thiab txias ntawm lub peev xwm adsorption ntawm CMK-3. Lub peev xwm adsorption ntawm CMK-3 nce nrog kub nce ntawm 30 txog 60◦C. Nws tau raug tshaj tawm tias qhov laj thawj tseem ceeb yog qhov chaw ua haujlwm tau nce nrog qhov kub thiab txias vim qhov endothermic xwm ntawm cov txheej txheem, thiab intra-particle diffusion ntawm cov adsorbents tau nce nrog qhov nce ntawm qhov kub ntawm adsorption (Peng li al., 2015) . Tsis tas li ntawd, kev txav mus los ntawm PhGs molecules tau nce ntxiv thiab lawv qhov kev tawm tsam tsis kam txo nrog qhov kub thiab txias. Lub peev xwm adsorption txo qis nrog qhov kub thiab txias thaum qhov kub ntawm 60-80 ◦C. Nws tau qhia tias qhov ntsuas kub adsorption muaj qhov zoo tshaj plaws thiab PhGs molecules yuav tsis ruaj khov ntawm qhov kub siab. Yog li ntawd, qhov zoo tshaj plaws adsorption kub yog xaiv los ntawm 60 ◦C.
Qhov zoo tshaj plaws adsorption tej yam kev mob ntawm CMK{{0}} yog raws li nram no: tus qauv concentration yog 0.41 mg/g, kua pH yog 6, thiab adsorption kub yog 60◦C. Nyob rau hauv cov xwm txheej zoo, lub peev xwm adsorption ntawm PhGs nyob rau hauv crude extract ntawm CMK -3 yog 358.09 ± 4.13 mg / g, uas yog ntau tshaj peb npaug ntawm adsorption peev ntawm PhGs ntawm macroporous resins (HPD300, 94.93 mg / g) (Liu et al., 2013). Lub caij no, tus nqi desorption ntawm CMK-3 yog 94.67 feem pua siab dua li ntawm macroporous resin.
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Adsorption Isotherms Cov
Cov adsorption isotherms tau pom hauv daim duab 8A. Nrog rau qhov nce ntawm qhov sib npaug ntawm qhov sib npaug, lub peev xwm adsorption rau PhGs tau nce thiab mus txog qhov ua kom saturation. Txhawm rau nkag siab ntxiv txog kev ua haujlwm adsorption ntawm PhGs ntawm CMK- 3, cov adsorption isotherms ntawm CMK-3 tau tshawb xyuas siv Langmuir thiab Freundlich qauv. Cov tsis muaj adsorption tau txais los ntawm cov qauv sib txawv muab qee cov ntaub ntawv tseem ceeb ntawm adsorption mechanisms. Daim duab 8B, C piav qhia txog CMK-3 adsorption isotherms qauv los ntawm Langmuir thiab Freundlich qauv.
Tus qauv Langmuir tau ua raws li qhov kev xav tias adsorption tshwm sim ntawm qhov chaw tshwj xeeb hauv cov adsorbent, tsis muaj kev cuam tshuam tseem ceeb ntawm cov adsorbed hom, thiab cov adsorbent tau saturated tom qab ib txheej ntawm:
adsorbent molecules tsim rau ntawm qhov chaw adsorbent. Linearized Langmuir isotherm equation tuaj yeem sau tau raws li hauv qab no:
CE/QE=1 KLqm plus CE/QM (2)
Tus qauv Freundlich feem ntau yog siv los piav txog cov yam ntxwv adsorption ntawm ntau txheej thiab ntau qhov chaw (Wu li al., 2017). Nws daim ntawv linearized yog muab raws li hauv qab no:lnqe=lnKF plus 1n/lnce (3)
Qhov twg QM (mg / g) yog qhov theoretical siab tshaj plaws monolayer adsorption muaj peev xwm, KL (mL / mg) yog Langmuir tas li hais txog lub zog adsorption uas cuam tshuam txog kev sib raug zoo ntawm cov adsorbate thiab adsorbent (Wu li al., 2017; He et al. , 2019), KF [(mg/g)•(mL/mg)1/n] thiab n yog Freundlich constants. KF yog qhov taw qhia ntawm tus txheeb ze adsorption muaj peev xwm, n muaj feem xyuam rau qhov loj ntawm cov adsorption tsav tsheb thiab heterogeneity ntawm qhov chaw sib khi, thiab 1 / n qhia qhov zoo ntawm adsorption.
Cov yam ntxwv ntawm cov qauv isotherm tau sau tseg hauv Table 3. Cov qauv Langmuir tau zoo dua li Freundlich qauv los piav qhia cov ntaub ntawv adsorption ntawm PhGs ntawm CMK-3, uas qhia tau hais tias qhov adsorption ntawm phenylethanoid glycosides ntawm CMK{{ 2}} yog ib qho yooj yim monolayer adsorption txheej txheem (Wang F. li al., 2017). Tsis tas li ntawd, muaj cov pa oxygen-muaj cov pab pawg ua haujlwm nyob rau saum npoo ntawm CMK-3 txhim kho cov adsorption ntawm PhGs. Qhov siab tshaj plaws adsorption tus nqi (QM) tuaj yeem ncav cuag 380.70 mg / g uas zoo ib yam li tus nqi sim. Tsis tas li ntawd, 1 / n tus nqi ntawm PhGs tau suav tias yog 0.22, uas yog<0.5. it="" indicated="" that="" the="" adsorption="" of="" the="" phgs="" on="" the="" cmk-3="" could="" take="" place="" easily="" (fu="" et="" al.,="" 2007;="" gan="" et="" al.,="" 2018).="" this="" indicated="" that="" the="" adsorption="" of="" phenylethanoid="" glycosides="" on="" cmk-3="" was="" not="" completely="" a="" physical="" adsorption="" process.="" phgs="" contain="" multiple="" hydroxyl="" groups="" which="" might="" form="" hydrogen="" bonds="" with="" the="" oxygen-containing="" functional="" groups="" on="" the="" cmk-3="" and="" offer="" weak="" chemical="" adsorption="" for="" the="" adsorption.="" therefore,="" cmk-3="" adsorption="" of="" phgs="" was="" a="" complex="" adsorption="" process="" combining="" physical="" adsorption="" with="">
Adsorption Kinetics
CMK-3 adsorption muaj peev xwm raug tshawb xyuas raws li lub sijhawm ua haujlwm los txiav txim siab lub sijhawm adsorption equilibrium hauv daim duab 9A. Kev tshawb fawb qhia tau hais tias qhov sib npaug adsorption tus nqi maj mam txo qis thiab maj mam txo qis thaum lub peev xwm adsorption nce mus txog qhov sib npaug. Nws tau pom tias qhov sib npaug adsorption tau mus txog tom qab 14 teev. Tus nqi pib sai sai tuaj yeem yog vim qhov siab concentration gradient ntawm PhGs thiab CMK-3 hauv kev daws teeb meem thiab qhov chaw ntawm CMK-3 muaj ntau qhov chaw ua haujlwm.
Adsorption kinetics tau soj ntsuam los ntawm daim ntawv thov ntawm pseudo-thawj-order, pseudo-thib ob-order, thiab intra-particle diffusion qauv. Cov duab kos duab ntawm pseudo-thawj-order, pseudo-second-order qauv, thiab intra-particle diffusion qauv rau adsorption PhGs mus rau CMK-3 tau pom nyob rau hauv daim duab 9B-D, feem.
Cov kab ntawv ntawm cov qauv kinetics tau nthuav tawm raws li hauv qab no: Pseudo-thawj-order (Li li al., 2017a):
log (qe − qt )=logqe − K1/2.303 t (4)
Pseudo-thib ob-order (Tang et al., 2018):
t/qt=1/K2q2e plus t/qt (5)
qhov twg K1 (1/min) thiab K2 (g/mg·min−1) yog tus nqi tsis tu ncua ntawm pseudo-first-order tus nqi sib npaug thiab pseudo-thib ob-order tus nqi sib npaug, feem. qe (mg / g) yog theoretical adsorption muaj peev xwm ntawm equilibrium.
Intra-particle difffusion qauv:
qt=Kpt1/2ntxiv rau C (6)
qhov twg Kp yog qhov intra-particle difffusion tas li (mg / g min) thiab C yog qhov cuam tshuam ntawm ciam txheej cuam tshuam (mg / g).
Cov nqi suav ntawm qe, tus nqi tsis tu ncua, thiab kev sib raug zoo coefficient yog qhia nyob rau hauv Table 4. Tus qauv pseudo-thib ob-order tau muab tus nqi R2 ntawm 0.998, thaum tus nqi R2 ntawm tus qauv pseudo-thawj thawj yog 0.84. Nws txhais tau hais tias tus qauv pseudo-thib ob-txiav txim siab nthuav tawm txoj kev sib raug zoo dua li tus qauv pseudo-thawj thawj-order. Daim duab 9C qhia tau hais tias cov phiaj xwm kab ntawm t/qe vs. t qhia pom zoo nrog cov ntaub ntawv sim los ntawm cov qauv pseudo-thib ob-kev txiav txim. Qhov xam qe ntawm pseudo-thib ob-kev txiav txim tau zoo ib yam li cov ntaub ntawv sim (Table 4). Cov txiaj ntsig no tau qhia tias pseudo-thib ob-kev txiav txim yog tus qauv kinetics zoo dua li pseudo-thawj-order.
Txawm li cas los xij, qhov kev ntsuam xyuas cov txiaj ntsig ntawm tus qauv pseudo-thib ob-kev txiav txim tsis tuaj yeem txiav txim siab lub peev xwm adsorption mechanism. Nws feem ntau tau lees paub tias adsorption kinetics tau tswj hwm los ntawm diffusion mechanism, uas muaj sab nraud diffusion, ciam txheej diffusion, thiab intra-particle diffusion (Wong et al., 2019). Tus qauv intra-particle diffusion tau siv los txiav txim siab tias intra-particle diffusion yog tus nqi-limiting kauj ruam.
Lub intra-particle diffusion tau hais tias yog tus nqi txwv cov kauj ruam thaum qt vs. t1/2 yog linear. Lub intra-particle diffusion yog tib theem tswj tus nqi thaum lub nkhaus hla los ntawm lub hauv paus chiv keeb. Nws tuaj yeem pom los ntawm daim duab 9D, tias qhov sib txawv ntawm qhov sib txawv tau tswj hwm los ntawm ob theem sib txawv. Thawj theem ntawm qhov nkhaus sawv cev rau saum npoo adsorption. Qhov thib ob theem qhia intra-particle diffusion hauv CMK-3 pores. Raws li intra-particle diffusion zaj duab xis tsis dhau los ntawm lub hauv paus chiv keeb, tus qauv qhia tau hais tias lub adsorption mechanism yog ntau tshaj ib tug mechanism thiab intra-particle diffusion tsis yog tus nqi-limiting kauj ruam xwb. Yog li, nws tuaj yeem txiav txim siab tias cov txheej txheem ntawm PhGs adsorption ntawm CMK-3 yog qhov nyuaj uas ob qho tib si sab nraud adsorption thiab intra-particle diffusion tshwm sim ib txhij.
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Kev sim rov ua dua
Reusability yog ib qho tseem ceeb rau kev txiav txim siab siv thiab tus nqi ntawm adsorbents hauv kev siv tswv yim. Yog li ntawd, qhov kev ua tau zoo ntawm cyclic adsorption ntawm CMK -3 rau cov extract crude tau sim. Daim duab 10 tau pom cov txiaj ntsig ntawm cyclic adsorption ntawm CMK-3 rau ib qho crude extract ntawm Cistanche tubulosa. Nws tuaj yeem pom los ntawm daim duab 10 tias lub peev xwm adsorption ntawm CMK -3 hloov los ntawm 358.09 ± 4.13 mg / g rau 320.78 ± 5.62 mg / g tom qab peb lub voj voog ntawm adsorption, uas qhia tias CMK- 3 tau zoo rov ua dua, thiab CMK-3 tuaj yeem rov siv dua los ua kom cov phenylethanoid glycosides.
Cov lus xaus
Cov khoom adsorption ntawm PhGs ntawm peb hom mesoporous carbons tau tshawb xyuas thiab cov pa roj carbon mesoporous ua ntej thiab tom qab adsorption yog yam ntxwv. Cov txiaj ntsig tau pom tias CMK{{0}} muaj thaj chaw tshwj xeeb tshaj plaws thiab qhov pore ntim ntawm peb qhov adsorbents (CMK- 3, DMC, thiab CMK-8), thiab nws tuaj yeem nqus tau. PhGs molecules zoo dua DMC thiab CMK-8 los ntawm cov extracts ntawm Cistanche tubulosa. Vim tias cov pab pawg ua haujlwm muaj pa oxygen nyob rau saum npoo ntawm CMK-3 tuaj yeem muab ntau qhov chaw ua haujlwm adsorption rau PhGs molecules. Tsis tas li ntawd, hydrogen bonds tau tsim los ntawm cov pab pawg hydroxyl ntawm PhGs thiab cov pa oxygen-muaj cov haujlwm ua haujlwm ntawm CMK- 3. Lub peev xwm adsorption ntawm crude extract rau PhGs yog 358.09 ± 4.13 mg / g ntawm qhov pom kev zoo ntawm 0.41 mg / L, pH=6 thiab 60◦C, thiab tus nqi desorption ntawm CMK-3 yog 95.02 feem pua. Cov ntaub ntawv adsorption tau nthuav tawm tias adsorption ntawm PhGs ua raws li Langmuir qauv thiab pseudo-thib ob-order qauv, cov qauv intra-particle diffusion qhia tias tus nqi-txheej cov kauj ruam ntawm adsorption yog cov qauv intra-particle diffusion. CMK-3 tuaj yeem siv los ua qhov muaj peev xwm adsorbent kom muaj txiaj ntsig zoo adsorb PhGs los ntawm Cistanche tubulosa.
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DATA AVAILABILITY STATEMENT
Tag nrho cov ntaub ntawv tsim tawm rau txoj kev tshawb fawb no suav nrog hauv kab lus / cov khoom siv ntxiv.
AUTHOR CONTRIBUTIONS
HX tau ua txhua yam kev sim, tau sau cov ntaub ntawv, tshuaj xyuas cov ntaub ntawv, thiab sau cov ntawv sau. WP tau pab txheeb xyuas cov ntaub ntawv FT-IR thiab tau sau cov ntaub ntawv. XL tau pab piav qhia qee qhov kev sim ntsuas thiab kho cov ntawv sau. JZ tau pab txhawb rau kev txhais lus tshawb fawb ntawm cov txiaj ntsig thiab kho cov ntawv sau.
NYIAJ
Peb ua tsaug txaus siab lees paub txog kev txhawb nqa los ntawm National Natural Science Foundation rau Cov Kws Tshawb Fawb Hluas ntawm Tuam Tshoj (Grant No. 21706166), Program rau Young Innovative Talents ntawm Shihezi University (CXRC201802), Program rau Changjiang Scholars thiab Pab Pawg Tshawb Fawb Tshiab hauv University (Grant No. . IRT_15R46), thiab Yangtze River scholar research project ntawm Shihezi University (Grant No. CJXZ201601)
