High-phosphorus Diet Induces Upregulation Ntawm Bmp2 Thiab Spp1 MRNA Qhia hauv Rat Raum Ⅱ

Kev sib tham

Ib qho kev paub zoo ntawm lub cev muaj txiaj ntsig ntawm kev noj zaub mov siab P yog qhovkev loj hlob ntawm nephrocalcinosis. Hauv kev tshawb nrhiav tam sim no,cev Cathiab P concentrations tau nce nyob rau hauv high-P pawg piv rau pawg tswj hwm, uas zoo ib yam nrog cov txiaj ntsig ntawm kev tshawb fawb yav dhau los1-6). Cov kev tshawb fawb yav dhau los tau tshaj tawm tias cov qhab nia histological ntawm nephrocalcinosis tau zoocorrelated nrog raum Cathiab P concentrations4, 21). Yog li, txawm hais tias tsis muaj kev soj ntsuam histological ntawm lub raum tau ua tiav hauv txoj kev tshawb no,nce raum Cathiab P concentrations qhia tiasnephrocalcinosistau induced los ntawm high-P noj.

Nyem qhov no kom tau txais Natural organic CISTANCHE EXTRACT nrog 25% ECHINACOSIDE thiab 9% ACTEOSIDE rau lub raum ua haujlwm

Fig. 1 Renal mRNA qhia ntawm Bmp2 (A) thiab Spp1 (B) hauv kev tswj (qhib bar) thiab high-P (khoom dub bar). Tus nqi ntawm pawg tswj hwm tau suav tias yog 1.00. Tus nqi yog txhais tau tias ± SEM (n ＝ 6). * Ntau qhov sib txawv ntawm pawg tswj hwm (P ＜ 0.05).

Vim tias Ca deposits induced los ntawm high-P noj zaub mov feem ntau xav tias yog ib daim ntawv ntawm hydroxyapatite6, 7), peb xav hais tias txoj kev loj hlob ntawm nephrocalcinosis yog tswj los ntawm ib tug zoo xws li cov txheej txheem ntawm kev kho mob.osteogenesis. Yog li, peb tau tshuaj xyuas qhov cuam tshuam ntawm kev noj zaub mov siab P ntawm Bmp2 mRNA qhia hauv lub raum. Cov txiaj ntsig tau pom tiascev bmp2mRNA qhia tau nce nyob rau hauv high-P pab pawg neeg thiab hais tias ib tug high-P noj zaub mov induces upregulation ntawm Bmp2 mRNA qhia nyob rau hauv lub raum. Qhov kev tshawb pom no tuaj yeem pab suav txog cov txheej txheem ntawm kev txhim kho nephrocalcinosis vim yog kev noj zaub mov siab-P. Txawm hais tias cov pov thawj tseeb txhawb peb qhov kev xav tsis muaj, ib qho ua tau yog tias qhov kev qhia ntawm osteogenic transcription yam yog nce siab hauv thaj tsam calcified hauv lub raum. BMP2 induces osteoblast sib txawv thiab cov pob txha tsim los ntawm kev tswj hwm osteogenic transcription yam xws li Osterix, Runx2, thiab Msx28, 9); Yog li, BMP2 tuaj yeem txhawb nqa nephrocalcinosis los ntawm kev tswj hwm ntawm xws liosteogenic transcriptionyam. Tam sim no, lub luag haujlwm ntxaws ntawm BMP2 hauv kev txhim kho nephrocalcinosis tseem tsis tau meej. Tsis tas li ntawd, txij li tsuas yog Bmp2 mRNA qhia tau ntsuas hauv qhov kev tshawb fawb no, nws tsis paub tias puas muaj qhov sib txawv ntawm BMP2 protein qhia. Txawm li cas los xij, peb cov txiaj ntsig tau qhia tias muaj peev xwm ua tau los ntawm kev hloov pauv hauv BMP2 qhia ua lub luag haujlwm muaj zog hauv cov kab mob ntawm high-P kev noj haus-induced nephrocalcinosis.

Txoj kev tshawb no tau tsom mus rau Spp1 mRNA qhia ntxiv rau Bmp2 mRNA qhia. Peb txoj kev tshawb fawb yav dhau los uas siv cov tshuaj tiv thaiv kab mob immunohistochemical qhia tau hais tias muaj ntau ntau ntawm OPN qhia nyob rau hauv lub raum tubules ntawm nas noj cov zaub mov siab-P12). Txoj kev tshawb fawb tam sim no pom tias Spp1 mRNA qhia tau nce los ntawm kwv yees li 10-fold hauv high-P pawg. Cov txiaj ntsig ntawm cov kev tshawb fawb tam sim no thiab yav dhau los qhia tau hais tias kev noj zaub mov siab-P ua rau OPN qhia hauv lub raum. Ntawm qhov tod tes, lwm cov kws tshawb fawb tau pom tias OPN ua lub pob zeb matrix yog qhov tseem ceeb hauv kev tsim cov pob zeb tso zis22, 23); Yog li, peb tsab ntawv tshaj tawm yav dhau los tau qhia tias OPN tuaj yeem muaj lub luag haujlwm raws li cov khoom siv matrix hauv kev tsim cov Ca deposits induced los ntawm high-P noj 12). Txawm li cas los xij, Paloian et al. tsis ntev los no tau tshaj tawm tias OPN ua lub luag haujlwm hauv kev tiv thaiv kev noj zaub mov siab-P nephrocalcinosis hauv OPN KO nas24). Namely, lub physiological lub luag hauj lwm ntawm OPN ntawm high-P noj nephrocalcinosis zoo li yuav tsub kom es ib tug nce qib ntawm calcification nyob rau hauv lub raum. Tsis tas li ntawd, cov ntaub ntawv dhau los tau piav qhia tias OPN qib nce ntxiv los cuam tshuam ntxiv pathologic ectopic calcification nyob rau hauv lub raum thiab vasculature raws li OPN yog upregulated ntawm qhov chaw ntawmpathologic calcification24, 25).

Raws li tau hais los saum no, txoj kev tshawb fawb tam sim no tau qhia qhov tseem ceeb ntawm BMP2 thiab OPN hauvHigh-P kev noj haus-induced nephrocalcinosis. Nws yog ib qho tseem ceeb uas cov teebmeem ntawm BMP2 thiab OPN ntawm txoj kev loj hlob ntawm nephrocalcinosis yog xav tias yuav tswj hwm los ntawm cov txheej txheem sib txawv. Cov kev sib txawv ntawm cov tshuab no yuav qhia tau tias lub hauv paus txheej txheem ntawm nephrocalcinosis yog qhov nyuaj.

Cov ntsiab lus tom ntej no yuav tsum xav txog yog tias kev loj hlob inhibition tuaj yeem cuam tshuam rau Bmp2 thiab Spp1 mRNA qhia hauv lub raum vim tias txoj kev tshawb fawb tam sim no tau pom qhov txo qis ntawm lub cev qhov hnyav kawg hauv pawg P siab. Kev soj ntsuam hauv peb yav dhau los12) thiab cov kev tshawb fawb tam sim no tuaj yeem pab suav txog qhov no. Peb yav dhau los tau tshaj tawm txog kev nthuav tawm OPN protein ntau hauv lub raum tubules ntawm cov nas uas nws txoj kev loj hlob tsis cuam tshuam los ntawm high-P noj 12). Tsis tas li ntawd, txoj kev tshawb fawb tam sim no tau pom tias muaj kev loj hlob inhibition hauv cov pab pawg P siab, tab sis qib siab ntawm Spp1 mRNA qhia hauv lub raum. Raws li cov kev soj ntsuam no, txoj kev tshawb fawb tam sim no zoo li muaj kev cuam tshuam me ntsis ntawm kev loj hlob inhibition ntawm Bmp2 thiab Spp1 mRNA qhia. Txawm li cas los xij, cov ntsiab lus ntawm kev sib raug zoo ntawm kev loj hlob inhibition thiab Bmp2 thiab Spp1 noob qhia tseem tsis meej. Yog li ntawd, kev tshawb nrhiav ntxiv yog xav tau los piav qhia qhov no. Hauv kev xaus, kom paub meej txog cov txheej txheem ntawm kev loj hlob ntawm nephrocalcinosis tshwm sim los ntawm kev noj zaub mov siab P, txoj kev tshawb fawb tam sim no tau txiav txim siab qhov cuam tshuam ntawm kev noj zaub mov siab P ntawm Bmp2 thiab Spp1 mRNA qhia hauv lub raum. Lub raum Ca thiab P concentrations tau nce siab hauv pawg siab P piv rau pawg tswj hwm. Tsis tas li ntawd, mRNA kev qhia ntawm Bmp2 thiab Spp1 tau nce siab hauv pawg P siab piv rau pawg tswj hwm. Cov txiaj ntsig no qhia tau hais tias kev noj zaub mov P siab txhawb kev tswj hwm ntawm Bmp2 thiab Spp1 mRNA qhia hauv lub raum.

Cov ntaub ntawv

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