Herba Cistanche Extract Enhances Mitochondrial ATP Generation hauv Rat Hearts Thiab H9c2 Cells
Mar 04, 2022
Hu rau: Audrey Huaudrey.hu@wecistanche.com
Hoi Yan Leung & Kam Ming Ko
Hais txog tsab xov xwm no: Hoi Yan Leung & Kam Ming Ko (2008) Herba Cistanche Extract Enhances Mitochondrial ATP Generation in Rat Hearts and H9c2 Cells, Pharmaceutical Biology, 46:6, 418-424, DOI: 10.1080/138380508
Abstract
Txhawm rau tshawb xyuas cov tshuaj hauv paus ntawm "Yang- invigorating" kev ua ntawm Herbal Cistanche [cov nroj tsuag qhuav tag nrho ntawm Cistanche deserticola YC Ma (Orobanchaceae)], hauv Suav tshuaj, cov teebmeem ntawm methanol extract ntawm Herba Cistanche ntawm mitochondrial ATP tiam muaj peev xwm tau tshuaj xyuas siv tus ex vivo nas lub plawv qauv thiab ib qho hauv situ H9c2 cell assay. Kev kho mob nrog Herba Cistanche extract nce myocardial mitochondrial ATP tiam muaj peev xwm nyob rau hauv ib koob tshuaj nyob rau hauv nas, raws li kev soj ntsuam nyob rau hauv vitro ntsuas. Qhov kev txhawb nqa ntawm ATP tiam muaj peev xwm tau cuam tshuam nrog kev sib txuas ntxiv hauv mitochondrial electron thauj khoom txhawb nqa los ntawm pyruvate tab sis tsis yog succinate. Kev kho mob Herba Cistanche kuj tau nce myocardial mitochondrial complex I thiab complex III kev ua ub no, nrog rau qhov kev txhawb nqa ntawm complex I kev ua si loj dua. Herba Cistanche kev kho mob tau tsim ib koob tshuaj-thiab lub sij hawm-nyob ntawm qhov nce hauv mitochondrial ATP tiam muaj peev xwm hauv H9c2 hlwb. Cov txiaj ntsig tau qhia tias Herba Cistanche kev kho mob tuaj yeem nce mitochondrial ATP tiam hauv nas lub siab ex vivo thiab H9c2 hlwb nyob rau hauv situ, tejzaum nws los ntawm kev txhim kho oxidative phosphorylation.
Ntsiab lus:ATP, Cistanche deserticola, H9c2 hlwb, lub plawv, mitochondria.
Taw qhia
"Yang invigoration," uas embodies lub generalized li ntawm lub cev muaj nuj nqi nyob rau hauv Suav tshuaj, koom nrog rau kev siv lub zog nyob rau hauv tsav ntau yam biochemical dab. Hauv qhov no, ATP, uas yog lub txiaj ntsig thoob ntiaj teb ntawm lub zog hauv kev ua haujlwm ntawm tes, feem ntau yog tsim los ntawm oxidative phosphorylation hauv mitochondria. Peb tau tshaj tawm tias "Yang invigorating" tshuaj ntsuab muaj peev xwm nce mitochondrial ATP tiam muaj peev xwm (Ko li al., 2004). Qhov no yog kev txhawb nqa los ntawm peb qhov kev tshawb pom tsis ntev los no tias "Yang- invigorating" tab sis tsis yog "Yin-nourishing" Suav tshuaj ntsuab tuaj yeem txhim kho myocardial ATP tiam muaj peev xwm hauv nas lub siab ex vivo (Ko et al., 2006). Herba Cistanche, qhuav tag nrho cov kab mob parasitic (tsis suav cov paj) ntawm Cistanche deserticola YC Ma (Orobanchaceae), yog cais raws li 'Yang-invigorating' tonifying tshuaj ntsuab nyob rau hauv cov tshuaj suav tshuaj (Chen, 1998), thiab nws zoo nkaus li nrov tshaj plaws. muaj nyob rau hauv ib tug xov tooj ntawm Suav patent formulations siv rau Yang-invigoration'. Hauv Suav teb thiab Nyij Pooj, Herba Cistanche tau siv dav rau kev kho mob ntawm tus tswv ntawm Yang deficiency cov tsos mob. Cov kev tshawb fawb pharmacological tau qhia tias Herba Cistanche tuaj yeem tshem tawm cov dawb radicals (Xiong li al., 1996), tsim cov tshuaj sedative (Lu, 1998), antiaging (Lee et al., 1990), antinociceptive thiab anti-inflammatory teebmeem (Lin et al., 2002). ), thiab txhim khu kev tiv thaiv kab mob (Wu et al., 2005). Lub ntsiab active ingredient ntawm Herba Cistanche yog phenylethanoid glycosides (Ouyang li al., 2003), uas tau pom muaj antibacterial, antistress, thiab antioxidative zog (Xiong li al., 1998), raws li zoo raws li anti-apoptotic teebmeem ntawm cultured. neurons (Tian & Pu, 2005). Hauv txoj kev tshawb fawb tam sim no, txhawm rau tshawb xyuas cov tshuaj hauv paus ntawm Yang-invigorating kev txiav txim ntawm Herba Cistanche, cov teebmeem ntawm Herba Cistanche kev kho mob ntawm ATP tiam muaj peev xwm hauv mitochondria cais tawm ntawm nas lub siab ex vivo thiab kab lis kev cai H9c2 cardiomyocytes nyob rau hauv situ. Txhawm rau tshawb xyuas cov txheej txheem biochemical hauv qab ntawm ATP tiam-txhim kho qhov ua tau zoo, cov teebmeem ntawm Herba Cistanche kev kho mob ntawm mitochondrial electron thauj thiab complex I-IV kev ua ub no kuj tau tshuaj xyuas hauv lub siab nas.
Cistanche deserticola extract
Cov ntaub ntawv thiab cov txheej txheem
Tshuaj, kab lis kev cai ntawm tes, thiab tshuaj ntsuab cov ntaub ntawv ATP, ADP, thiab 3-[4,5-dimethylthiozol-2-yl]-2,5-diphenyl- tetrazolium bromide ( MTT) tau yuav los ntawm Sigma Chemical Co. (St. Louis, MO, USA). Luciferase tov (ATPlite) tau txais los ntawm PerkinElmer (Boston, MA, USA). Dulbecco's modified Eagle's medium (DMEM) thiab fetal bovine serum (FBS) tau yuav los ntawm GIBCO BRL Life Technologies (Grand Island, NY, USA). H9c2 xov tooj ntawm tes tau yuav los ntawm ATTC (Rockville, MD, USA). Herba Cistanche tau muab los ntawm ib tus neeg muag tshuaj ntsuab hauv zos (Lee Hoong Kee). Cov tshuaj ntsuab tau lees paub los ntawm tus neeg muag khoom, thiab daim ntawv pov thawj (HKUSTY00301) tau muab tso rau hauv Department of Biochemistry, Hong Kong University of Science & Technology (HKUST).
Tshuaj ntsuab extraction
Herba Cistanche (100 g) txiav rau hauv me me pieces thiab ces muab rho tawm los ntawm cua sov nyob rau hauv reflux nyob rau hauv 300 mL methanol.
ntawm 65 ◦C rau 2 h. Cov txheej txheem tau rov ua dua ob zaug. Cov kua dej sib tov tau qhuav los ntawm evaporating cov kuab tshuaj nyob rau hauv lub siab txo, thiab cov methanol extract ntawm Herba Cistanche tau los ntawm ib tug yield ntawm 39 feem pua (w/w). Kev tshuaj xyuas tshuaj tau qhia tias methanol ex-tract ntawm Herba Cistanche muaj tag nrho cov saponins, tag nrho cov flavonoids, tag nrho lignans, thiab polysaccharides ntawm concentrations ntawm 1.62 feem pua (w/w), 0.08 feem pua, 0.15 feem pua, thiab 75.6 feem pua.
Kev tu tsiaj
Cov txiv neej laus Sprague-Dawley nas (8-10 lub lis piam; 250-300g) tau khaws cia nyob rau hauv 12-h tsaus ntuj / lub teeb lub voj voog nyob rau hauv ib chav huab cua / av noo ntawm li 22◦C thiab pub zaub mov thiab dej ad libitum nyob rau hauv Cov Tsev Kho Mob Tsiaj
ntawm HKUST. Cov txheej txheem sim tau pom zoo los ntawm Pawg Neeg Saib Xyuas Kev Tshawb Fawb ntawm HKUST.
Tshuaj kho mob
Tsiaj txhu tau muab faib ua pawg, nrog tsib mus rau rau tus tsiaj nyob rau hauv txhua. Hauv pab pawg kho mob, cov nas tsuag tau muab tshuaj ua ke nrog cov tshuaj methanol extract ntawm Herba Cistanche (solved / suspended hauv dej) ntawm kev noj tshuaj txhua hnub ({{0}}}.031–0.5 g / kg) rau 3 hnub. Tswj tsiaj tau txais dej xwb. Nees nkaum plaub teev tom qab txhaj tshuaj kawg, lub plawv tau txais los ntawm phenobarbital-anesthetized tsiaj thiab raug rau biochemical tsom xam.
Kev npaj ntawm mitochondrial feem thiab ntsuas ntawm ATP tiam muaj peev xwm (ATP-GC) ex vivo
Lub plawv sab laug ventricular cov qauv kuaj tau raug tshem tawm thiab yaug nrog dej khov-txias isotonic buffer (0.32 M sucrose, 1 mM EDTA, 50 mM Tris / HCl, pH 7.4). Cov kab mob plawv mitochondrial tau npaj los ntawm qhov sib txawv centrifugation hauv iso-tonic tsis nyob ntawm 4◦C. Ib qho 10 feem pua (w/v) cardiac homogenate tau npaj los ntawm homogenizing cov nqaij minced ventricular nrog Teflon-iav homogenizer ntawm 4000 rpm rau 20-30 ua tiav strokes. Cov homogenate tau centrifuged ntawm 600 g rau 10 min kom tshem tawm cov nuclei thiab cov khib nyiab ntawm tes. Tom qab ntawd tus supernatant tau centrifuged ntawm 8000 g rau 30 feeb kom sediment lub mitochondria (Evan, 1992). Cov pellets tau rov ua dua nyob rau hauv 1 mL ntawm isotonic tsis thiab rov tsim cov mitochondrial feem. Lub mitochondrial ATP-GC ntawm cov tsiaj tsis kho tau ntsuas los ntawm txoj kev Leung li al. (2005).
Kev ntsuas ntawm mitochondrial electron thauj
Kev ntsuas ntawm kev thauj hluav taws xob hauv kev sib cais mitochondria, uas yog raws li kev txo qis ntawm MTT, tau ua raws li tau piav qhia los ntawm Cohen li al. (1997). Mitochondria tau npaj ntawm cov protein ntau ntawm 1 mg / mL nrog rau qhov tsis sib haum xeeb (250 mM sucrose, 50 mM HEPES, 10 mM KH2PO4, 2 mM MgCl2, 1 mM EGTA, pH 7.4) . Ib qho aliquot (40 µL) ntawm mitochondria tau tov nrog 100 µL txhua ntawm 15 mM pyruvate lossis 0.5 mM succinate thiab 0.42 mg / mL MTT. Cov tshuaj tiv thaiv sib xyaw yog incubated ntawm 37◦C rau 10 feeb nrog maj mam co. Tom qab incubation, cov tshuaj tiv thaiv sib tov raug txiav los ntawm qhov sib ntxiv ntawm 100 µL lysis buffer (10 feem pua, w/v, sodium dodecyl sulfate, thiab 45 feem pua dimethylformamide, kho rau pH 4.7 nrog glacial acetic acid). Tom qab sawv ntsug rau 5 feeb, kev nyeem ntawv absorbance ntawm cov tshuaj tiv thaiv sib tov tau muab coj los siv nrog lub microtiter phaj nyeem ntawv (Bio-Rad, Hercules, CA, USA) thiab tshaj tawm tias qhov sib txawv ntawm 570 nm thiab 630 nm. Cov ntaub ntawv tau nthuav tawm raws li feem pua ntawm cov txiaj ntsig ntawm kev tswj hwm (ie, Herba Cistanche-tsis kho) pawg.
Cell kab lis kev cai
H9c2 hlwb, ib kab ntawm tes mus tas li muab tau los ntawm lub plawv myoblasts (Hescheler li al., 1991), tau coj los ua monolayers hauv DMEM ntxiv nrog 10 feem pua (v/v) FBS. Qhov nruab nrab muaj cov piam thaj (4.5 g / L) thiab glutamine (4.5 mM), ntxiv nrog NaHCO3 (17 mM), penicillin (100 IU / mL), thiab streptomycin (100 μg / mL). Txhua lub hlwb tau loj hlob nyob rau hauv qhov chaw ntawm 5 feem pua (v/v) CO2 hauv huab cua ntawm 37◦C. Qhov nruab nrab tau hloov los ntawm qhov nruab nrab tshiab txhua 2 lossis 3 hnub. Ib qho khoom lag luam ntawm cov hlwb tau loj hlob hauv 75 cm2 kab lis kev cai haus cawv thiab sib cais ua ntej sib xyaw ntawm qhov sib piv ntawm subcultivation ntawm 1:10. Rau kev ntsuam xyuas ATP-GC, H9c2 hlwb tau cog rau ntawm qhov ntom ntawm 2.5 104 hlwb/zoo rau hauv 24-zoo phaj. Tom qab kev sib txuas ntawm tes, Herba Cistanche extract (solved nyob rau hauv phosphate-buffered saline; PBS) tau siv nyob rau hauv nruab nrab, thiab lub hlwb tau incubated rau lub sij hawm nce (2-16 h) ntawm lub sij hawm. Tswj (tsis kho) hlwb tau muab rau PBS nkaus xwb.
Kev ntsuas ntawm ATP-GC hauv qhov chaw
Tom qab lub sij hawm qhia ntawm incubation nrog Herba Cistanche extract ntawm nce concentrations (5{{10}}}-300 µg/mL), qhov kev ntsuam xyuas ATP-GC tau ua. Qhov nruab nrab yog aspirated thiab cov hlwb raug kho nrog digitonin (50 µg / mL) nyob rau hauv ib qho incubation buffer (120 mM KCl, 5 mM KH2PO4, 2 mM EGTA, 10 mM HEPES, 0.1 mM MgCl2, 0.5% BSA, pH 7.4). min ntawm 37◦C. Tom qab aspirating lub digitonin, glutamate (5 mM), malate (5 mM), thiab ADP (60µM) tau ntxiv rau cov hlwb rau mitochondrial ATP tiam, uas tau saib xyuas ntawm lub sijhawm nce ntxiv ntawm 0 txog 15 min. Cov tshuaj tiv thaiv raug txiav tawm los ntawm qhov sib ntxiv ntawm 60 µL ntawm perchloric acid (30 feem pua , w / v), thiab cov tshuaj tiv thaiv sib tov ces centrifuged ntawm 600g rau 10 min ntawm 4◦C. Ib qho aliquot (120 µL) ntawm supernatant yog tov nrog 90 µL ntawm 1.4 M KHCO3 rau neutralization. Cov sib tov tau centrifuged dua ntawm 600 g ntawm 4◦C, thiab cov supernatants raug ntsuas rau ATP cov ntsiab lus raws li tau piav qhia ua ntej. ATP-GC ntawm cov hlwb tsis kho tau kwv yees los ntawm kev suav thaj tsam nyob rau hauv qhov nkhaus ntawm daim duab (AUC1) plotting ATP generated (nmol / mg protein) tawm tsam lub sij hawm (0-15 min) thiab qhia nyob rau hauv arbitrary units. Rau Herba Cistanche-kho cov hlwb, AUC1 qhov tseem ceeb ntawm kev tsim lub sij hawm incubation (3, 5, 7, 10, thiab 15 min) tau normalized rau tus nqi tswj hwm tus nqi los ntawm cov qauv tsis kho thiab qhia raws li feem pua ntawm kev tswj hwm. Tom qab ntawd, thaj tsam hauv qab ntawm qhov nkhaus (AUC2) ntawm daim duab plotting feem pua ntawm kev tswj hwm qhov tseem ceeb tiv thaiv lub sij hawm incubation (3-15 min) raug xam thiab ex-pressed hauv arbitrary units. Cov ntaub ntawv ntawm Herba Cistanche-kho pab pawg tau qhia raws li feem pua ntawm kev tswj hwm los ntawm kev sib npaug:
[AUC2(HerbaCistanche − kho)/AUC2(untreated)] × 100 feem pua .
Kev ntsuas ntawm complex I-IV thiab citrate synthase cov dej num
Mitochondrial electron thauj saw (ETC) complex I-III cov dej num tau ntsuas los ntawm spectrophotometric txoj kev siv complex-specific substrates (NADH rau complex I, succinate rau complex II, thiab decylubiquinol rau complex III) raws li tau piav qhia (Grad & Lemire, 2004; Hsu et al., 2005; Mark et al., 2001). Complex IV kev ua haujlwm tau ntsuas los ntawm kev ntsuas cytochrome c oxidase cov khoom siv los ntawm Sigma. Mitochondrial citrate synthase kev ua haujlwm tau ntsuas los ntawm txoj kev Srere (1969).
Protein assay
Protein concentrations ntawm mitochondrial fractions thiab cell lysates tau txiav txim siab siv BioRad protein assay cov khoom siv bovine serum albumin raws li tus qauv ({{0}}}.038–0.600 mg/mL).
Kev txheeb cais
Tag nrho cov ntaub ntawv tau nthuav tawm raws li tus qauv kev ua yuam kev ntawm qhov nruab nrab (SEM). Lawv tau txheeb xyuas los ntawm ib txoj kev tsom xam ntawm qhov sib txawv (ANOVA). Post hoc ntau qhov kev sib piv tau ua tiav nrog LSD. P tus nqi<0.05 were="" regarded="" as="" statistically="">
Cov txiaj ntsig
Cov teebmeem ntawm Herba Cistanche kev kho mob ntawm myocardial mitochondrial ATP-GC hauv nas
Raws li pom nyob rau hauv daim duab 1, kev kho mob nrog Herba Cistanche ex-tract ntawm koob tshuaj mus txog rau 0.5 g/kg nce myocardial mitochondrial ATP-GC nyob rau hauv ib koob tshuaj nyob rau hauv nas, nrog rau qib ntawm stimulation yog 89 feem pua. ntawm ib koob ntawm 0.5 g/kg.
Cov teebmeem ntawm Herba Cistanche kev kho mob ntawm mitochondrial electron thauj hauv nas lub siab
Qhov kev thauj mus los ntawm electron hauv kev sib cais mitochondria tau ntsuas los ntawm kev saib xyuas qhov txo qis ntawm MTT. Lub substrate siv rau kev ntsuam xyuas yog pyruvate los yog succinate. Raws li pom nyob rau hauv daim duab 2a, Herba Cistanche kev kho mob koob tshuaj-raws li nce qhov ntawm mitochondrial electron thauj txhawb nqa los ntawm pyruvate hauv nas lub siab, nrog rau qhov zoo tshaj plaws stimulation pom ntawm koob ntawm 0.5 g / kg. Txawm li cas los xij, tsis muaj kev hloov pauv tseem ceeb ntawm qhov kev thauj mus los ntawm mitochondrial electron txhawb nqa los ntawm succinate tau kuaj pom hauv Herba Cistanche-kho lub siab nas (Fig. 2b).
Cov teebmeem ntawm Herba Cistanche kev kho mob ntawm mitochondrial ua pa nyuaj ua haujlwm hauv nas lub siab
Raws li pom nyob rau hauv daim duab 3, Herba Cistanche kev kho mob ntawm qhov pom zoo koob tshuaj ntawm 0.5 g / kg ho nce complex I (34 feem pua) thiab complex III (22 feem pua) kev ua ub no nyob rau hauv nas plawv. Kev ua haujlwm ntawm citrate synthase, ib qho enzyme tseem ceeb rau lub voj voog tricarboxylic acid, tsis cuam tshuam los ntawm kev kho mob Herba Cistanche (Fig. 3). Western blot tsom xam qhia tias qib protein ntawm complex I thiab III tsis cuam tshuam los ntawm Herba Cistanche kev kho mob (cov ntaub ntawv tsis qhia).
Lub sij hawm kawm ntawm Herba Cistanche-induced-hloov hauv mitochondrial ATP-GC hauv H9c2 hlwb
Kev hloov pauv hauv ATP-GC tau tshuaj xyuas hauv Herba Cistanche- kho cov hlwb (50 thiab 150 µg / mL) tom qab 2 txog 16 teev ntawm incubation. Raws li pom nyob rau hauv daim duab 4, Herba Cistanche kev kho mob tau nce ATP-GC nyob rau hauv lub sij hawm-dependent thiab biphasic yam nyob rau hauv H9c2 hlwb, nrog rau qhov siab tshaj plaws stimulation (44 feem pua thiab 89 feem pua, raws li) soj ntsuam tom qab 8 h incubation rau ob qho tib si tshuaj concentrations. ATP-GC tom qab ntawd rov qab mus rau qhov tsis muaj kev tswj hwm qhov tseem ceeb thaum lub sij hawm incubation tau dhau mus rau 16 teev. Ua ntej pib ntawm stimulation tshwm sim thaum lub hlwb raug kho nrog ntau dua concentration (150 µg / mL) ntawm Herba Cistanche extract.
Concentration-dependence ntawm Herba
Cistanche-induced nce hauv ATP-GC hauv H9c2 hlwb
H9c2 hlwb raug kho nrog kev nce ntxiv (50- 300 µg / mL) ntawm Herba Cistanche extract rau 4 h. Daim duab 5 qhia tau hais tias Herba Cistanche kev kho mob nce ATP- GC nyob rau hauv ib tug concentration-dependent yam, nrog rau qhov siab tshaj plaws stimulation (82 feem pua) pom nyob rau hauv lub concentration ntawm 300 µg / mL.
Cistanche extract nce hauv ATP ntau lawm
Kev sib tham
Lub upregulation ntawm cellular kev ua ub no pab txhawb lub cev muaj nuj nqi xws li cov leeg nqaij contraction thiab tiv thaiv kab mob los ntawm Yang invigoration yuav tsum tau nce nyob rau hauv kev noj ntawm ATP, uas yog nyob rau hauv lem txhawb los ntawm mitochondrial oxidative phosphorylation. Nyob rau hauv txoj kev tshawb no tam sim no, Herba Cistanche kev kho mob tau pom tias koob tshuaj-nyob ntawm seb yuav ua rau cov mitochondrial ATP tiam muaj peev xwm ex vivo hauv nas plawv. Qhov kev soj ntsuam no yog ua raws li peb qhov kev tshawb pom yav dhau los uas ntawm Yang-invigorating Suav tonifying tshuaj ntsuab, Herba Cistanche kev kho mob tsim ib tug loj npaum li cas ntawm stimulation ntawm mitochondrial ATP tiam nyob rau hauv nas lub siab (Ko li al., 2006). Kev txhim kho ntawm mitochondrial ATP tiam yog sib npaug los ntawm kev nce hauv mitochondrial electron thauj uas tau tsav los ntawm pyruvate tab sis tsis succinate, raws li kev soj ntsuam los ntawm kev txo qis ntawm MTT. Cov txiaj ntsig tau los ntawm kev ntsuas kinetic ntawm mitochondrial complex I-IV qhia tau hais tias kev ua ub no ntawm ob qho tib si complexes I thiab III tau nce hauv Herba Cistanche-kho lub siab, nrog rau qhov kev txhawb nqa ntawm txoj haujlwm I ua haujlwm loj dua li ntawm complex III. Raws li kev ua ntawm citrate synthase, ib qho tseem ceeb enzyme ntawm lub voj voog tricarboxylic acid, tsis muaj kev cuam tshuam, kev txhim kho ntawm mitochondrial ATP tiam muaj peev xwm los ntawm Herba Cistanche kev kho mob feem ntau yuav raug ntaus nqi los ntawm stimulation ntawm complex I thiab / los yog III kev ua ub no. Feem ntau ntawm cov kev cai ATP ntawm lub plawv tau ntsib los ntawm mitochondria, uas yuav luag tag nrho ntawm cov complex I (NADH)-txuas oxidation ntawm fatty acid thiab qabzib (Stanley li al., 1997; Taegtmeyer, 1998). Complex I, uas nthuav tawm cov haujlwm qis dua li ua lwm yam ua pa nyuaj, suav tias yog qhov tseem ceeb hauv kev tswj hwm ntawm oxidative phosphorylation. Yog li, qhov stimulation lossis inhibition ntawm complex I kev ua si tuaj yeem muaj kev cuam tshuam loj rau kev mob plawv. Txawm hais tias Western blot tsom xam tsis tau qhia txog kev hloov pauv hauv qib protein ntawm ob txoj hauv kev no (cov ntaub ntawv tsis qhia), qhov nce enzyme ua haujlwm yuav tshwm sim los ntawm kev hloov pauv ntawm cov protein thiol thiab / lossis lipid ib puag ncig (Davey li al., 1998; Paradies et al., 2004).
Herba Cistanche Extracttuaj yeem tsim cov ATP tiam-txhim kho cov nyhuv hauv kab lis kev cai H9c2 hlwb hauv qhov chaw. Qhov pib tshwm sim ntxov ntawm qhov kev ua haujlwm stimulatory uas tsim los ntawm Herba Cistanche kev kho mob, tshwj xeeb tshaj yog nyob rau hauv cov koob tshuaj siab, yog raws li kev soj ntsuam los ntawm ex vivo cov kev tshawb fawb uas tau qhia tias cov synthesis ntawm complex I thiab III proteins tsis zoo li koom nrog hauv ATP tiam-txhim kho. kev ua. Kev ua qauv qhia ntawm Herba Cistanche-induced enhancement nyob rau hauv ATP tiam nyob rau hauv situ mob txhawb txoj kev xav hais tias qhov nce nyob rau hauv mitochondrial ATP tiam muaj peev xwm, raws li kev ntsuam xyuas los ntawm ex vivo ntsuas, tej zaum tsuas yog muaj kev cuam tshuam ib tug parallel nyhuv uas ua los ntawm cov tshuaj kho nyob rau hauv vivo mob. Kev ntsuas ntawm ATP tiam nyob rau hauv cais mitochondria thiab kab lis kev cai siv malate thiab glutamate li substrates yog ib qho kev ntsuas tsis ncaj ntawm lub xeev 3 mitochondrial respiration (Chen li al., 2003). Siv tib qhov kev ntsuam xyuas bioluminescent ATP, tus nqi ntawm mitochondrial ATP tiam nyob rau hauv ntau cov ntaub so ntswg ntawm cov nas tsis kho, raws li tau qhia ua ntej hauv peb lub chaw kuaj mob (Leung li al., 2005), tau pom zoo nrog cov ntaub ntawv tsis ntev los no (Drew & Leeuwenburgh, 2003). ). Lub peev xwm ntawm ATP tau kwv yees los ntawm ob-kauj ruam kev sib koom ua ke ntawm lub sijhawm hloov pauv hauv ATP qib hauv ob qho tib si ex vivo thiab hauv situ kev soj ntsuam. Yog li ntawd, qhov kev pom zoo ntawm cov tshuaj stimulatory tshwm sim tau nthuav dav rau kev sib piv. Kev txhim kho ntawm ATP tiam muaj peev xwm los ntawm Herba Cistanche kev kho mob hauv nas plawv mitochondria ex vivo tau sib npaug los ntawm kev txhawb nqa ntawm ATP tiam hauv H9c2 hlwb hauv qhov chaw. Muab lub xub ntiag ntawm ATP tiam-txhim kho kev ua si hauv Yang-invigorating 'Suav tonifying tshuaj ntsuab, suav nrog Herba Cistanche, hauv nas lub siab ex vivo (Ko et al., 2006), cov txiaj ntsig tau qhia tias kev ntsuas ntawm ATP tiam muaj peev xwm hauv H9c2 hlwb hauv situ tej zaum yuav raug siv los ua tshuaj ntsuam xyuas rau Yang-invigorating Suav tonifying tshuaj ntsuab los yog tshuaj ntsuab mis.
Hauv kev xaus, kev kho mob nrog Herba Cistanche extract tuaj yeem txhim kho mitochondrial ATP tiam muaj peev xwm hauv nas lub siab ex vivo thiab hauv H9c2 hlwb hauv qhov chaw. Cov kev tshawb pom txhawb peb cov postulation tias lub hauv paus pharmacological ntawm Yang-invigoration cuam tshuam nrog kev txhim kho lub cev ua haujlwm los ntawm kev txhawb nqa mitochondrial ATP ntau lawm.
Herba Cistanche extract txhim khu lub mitochondrial ATP tiam muaj peev xwm
Cov ntaub ntawv
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