Extraction, Sib cais thiab tshuaj ntsuam xyuas ntawm Polysaccharides los ntawm Cistanche

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Sheng Wei & Gao Leining

Polysaccharidesyog ib qho tseem ceeb biopolymer compounds. Txawm tias qhov kev tshawb fawb ntawmpolysaccharidesnyob rau hauv kuv lub teb chaws pib lig, nyob rau hauv lub xyoo caum tsis ntev los no, nrog rau kev kawm ntawm membrane txoj kev xav thiab kev tiv thaiv kab mob, qhov tseem ceeb ntawmpolysaccharidesnyob rau hauv lub neej phenomena tau maj mam nrhiav tau. , Nws tau pom tiaspolysaccharidesyog cov tshuaj uas tsis yog-cytotoxic thiab muaj ntau yam kev ua haujlwm lom neeg. Kev tshawb fawb ntawm polysaccharides tau them nyiaj ntau dua. Tus sau tau ua ib txoj kev tshawb fawb txog cov khoom siv polysaccharide ntawmCistanchenyob rau hauv cov tshuaj suav tshuajCistanche, vam tias yuav ua rau muaj txiaj ntsig rau kev tshawb fawb ntawmcistanchepolysaccharides.

cistanchepolysaccharides

1 Khoom siv thiab khoom siv

Cistanche: muab los ntawm Inner Mongolia Alexa cov khoom siv tshuaj kho mob. Anhydrous qabzib siv tshuaj: muab los ntawm Tuam Tshoj National Institute rau Kev Tswj Xyuas Tshuaj thiab Khoom Siv Tshuaj lom neeg, infrared spectrophotometer: 599-B PE. Ultraviolet spectrophotometer: UV-260 Shimadzu, lub tshuab nqus tsev kub kom qhuav qhov cub: YB-IA Tianjin.

2 Cov txheej txheem thiab cov txiaj ntsig kev sim

2.1 Extraction, sib cais thiab refining

Txiav lubcistanche, ntxiv dej, boil rau 2 teev, lim. Ntxiv dej, boil lub residue rau 2 teev, lim, muab cov extracts. Siv txoj kev Se-wag tshem tawm cov protein. Tom qab cov extract yog concentrated, ethanol yog ntxiv rau rov qab los nag, thiab nws yog decolorized nrog H2O2 nyob rau hauv cov xwm txheej ntawm pH =g②2 kom tau ib yam khoom dawb dawb. Ntxuav nrog cov ethanol thiab diethyl ether tsis tu ncua, thiab muab tso rau hauv lub tshuab nqus tsev desiccator kom qhuav hauv qhov txo qis kom tau txais cov khoom lag luam tawm dawb.

polysaccharide los ntawmcistanche

2. 2 Kev tshuaj xyuas lub cev thiab tshuaj lom neeg

(1) Physicochemical zog: Cov khoom zoocistanchepolysaccharidesyog nyob rau hauv daim ntawv ntawm off-dawb hmoov. Nws yog soluble hauv dej. Nws yog tshwj xeeb tshaj yog soluble hauv dej kub. Insoluble nyob rau hauv high-concentration ethanol, acetone, ethyl acetate, chloroform, ether thiab lwm yam organic solvents. Lub aqueous tov ntawmcistanchepolysaccharideyog pob tshab thiab khov, cov tshuaj tiv thaiv ntawm iodine-potassium iodide yog qhov tsis zoo, cov tshuaj tiv thaiv ntawm ninhydrin tsis zoo, thiab cov tshuaj tiv thaiv ntawm anthrone thiab mono carbazole sulfate yog qhov zoo.

(2) Purity identification: siv silica gel nyias-txheej chromatography nrog concentrated ammonia dej: n-butanol; dej (40'60'5) los txhim kho, lubcistanchepolysaccharidetsuas muaj ib qho chaw xwb, daim ntawv chromatography siv Xinhua lim ntawv los tsim kom txog li 8 teev, Muab nws tawm thiab tshuab kom qhuav, stain nrog Q 5 feem pua ​​​​toluidine xiav ethanol tov, thiab tom qab ntawd yaug nrog 95 feem pua ​​​​ethanol kom txog thaum lub keeb kwm yav dhau tsis muaj xim. Qhov tshwm sim tau pom ib qho chaw.

(3) Ultraviolet spectroscopy tsom xam: Lub Bossicacistanchepolysaccharidetau npaj rau hauv cov tshuaj 500Vg/ml thiab luam tawm ntawm 200-400m nrog ib qho ultraviolet spectrophotometer. Tsis muaj tus yam ntxwv nqus ntawm nucleic acid (260rm) thiab protein (280nm) tau pom.

(4) Infrared spectroscopy tsom xam: Noj 2mg ntawm Bossicacistanchepolysaccharide, nias KB1 ntsiav tshuaj, luam theej duab infrared spectroscopy, tus yam ntxwv haum raws li: 3500m, 3000m, 1600m, 1400cm, 110m, 890m yog cov yam ntxwv nqus peaks ntawm -pyranoside daim ntawv cog lus.

(5) Kev txiav txim siab ntawm cov ntsiab lus: Siv txoj kev sulfuric acid-anthrone J colorimetric, anhydrous qabzib raws li cov khoom siv, cov ntsiab lus polysaccharide ntsuas yog 69.64 feem pua, thiab txoj kev sulfuric acid-carbazole ntsuas glucuronic acid cov ntsiab lus ntawm 18 12 feem pua. Tag nrho cov ntsiab lus qab zib yog 87.76 feem pua, thiab cov seem yog dej thiab tshauv, tsis muaj protein ntau thiab nucleic acid.

cistanchepolysaccharide

3 Kev sib tham

Nws qhia qhov tawm dawbpolysaccharidesrho tawm los ntawmcistanchethiab lub cev thiab tshuaj muaj zog ntawm covpolysaccharides. Vim muaj kev txwv, lub purity ntawm lubcistanchepolysaccharidestsis zoo txaus. Qhov no yuav yog cov kev taw qhia ntawm tus sau lub dag zog. Nyob rau tib lub sij hawm, cov monosaccharide muaj pes tsawg leeg thiab kev sib txuas ntawm covcistanchepolysaccharidesYuav yog qhov teeb meem uas tus kws sau ntawv txuas ntxiv mus mloog.

Cov ntaub ntawv

1 Phau ntawv Journal ntawm Shenyang College of Pharmacy. Vol. 2, nr 3, 1985, 234–240

2 Shanghai Institute of Materia Medica, Suav Academy ntawm Sciences. Extraction thiab sib cais ntawm cov khoom xyaw nquag ntawm Suav tshuaj ntsuab. Shanghai Science thiab Technology Xovxwm, 1983415 ~ 420

3 Yuan Xiao, thiab lwm yam. Cog Physiology thiab Biochemistry Experiment. Higher Education Press, 1983

(Hnub tau txais, Lub Ob Hlis 21, 2000)

polysaccharide muab rho tawm los ntawm cistanche