AbstractCov kev tiv thaiv ntawm daim tawv nqaij inflammatory kev ua ub no ntawm rose petal extracts tau piav nyob rau hauv peb kev tshawb fawb yav dhau los. Vim tias daim tawv nqaij o yog ze ze rau ntawm daim tawv nqaij laus, peb txoj kev tshawb fawb tau tshawb xyuas qhov cuam tshuam ntawm Rosa gallica petals ntawm daim tawv nqaij aging-txog kev ua ub no xws li daim tawv nqaij ntxuav hniav dawb thiab anti-wrinkle zog. Txhua tus qauv tau npaj los ntawm kev rho tawm siv qhov sib txawv ntawm ethanol los ntsuas qhov zoo tshaj plaws extraction tej yam kev mob rau cov ntawv thov kev lag luam. Aqueous 50 feem pua ​​(v/v) EtOH extract ntawm R. gallica petal ho suppressed tyrosinase kev ua, melanin ntau lawm, thiab hnub ci UV-induced matrix metalloproteinase -1, ib tug yam ntxwv ntawm wrinkle tsim. Tsis tas li ntawd, cov aqueous 50 feem pua ​​(v/v) EtOH extract tau pom cov nyhuv antioxidative siab tshaj plaws thiab muaj cov ntsiab lus flavonoid siab tshaj plaws, ua raws li cov lus qhia los tiv thaiv kev laus. Zuag qhia tag nrho, peb qhov kev tshawb pom qhia tias R. gallica petals extracts pom muaj kev tiv thaiv kev laus. Tsis tas li ntawd, 50 feem pua ​​​​ntawm EtOH extraction, tshwj xeeb, yog qhov zoo tshaj plaws rau kev tiv thaiv kev laus, thiab cov tshuaj tiv thaiv oxidative nrog rau kom tau txais cov ntsiab lus flavonoid siab tshaj plaws.

Raws li cov kev tshawb fawb cuam tshuam, cistanche yog ib hom tshuaj ntsuab uas hu ua "cov tshuaj ntsuab muaj txuj ci uas ua rau lub neej ntev". Nws lub ntsiab tivthaiv yog cistanoside, uas muaj ntau yam teebmeem xws li antioxidant, tiv thaiv kev mob, thiab kev tiv thaiv kev ua haujlwm. Cov txheej txheem ntawm cistanche thiab tawv nqaij dawb yog nyob rau hauv cov nyhuv antioxidant ntawm cistanche glycosides. Melanin nyob rau hauv tib neeg daim tawv nqaij yog tsim los ntawm oxidation ntawm tyrosine catalyzed los ntawm tyrosinase, thiab cov tshuaj tiv thaiv oxidation yuav tsum muaj kev koom tes ntawm oxygen, yog li cov pa-dawb radicals nyob rau hauv lub cev ua ib qho tseem ceeb cuam tshuam rau melanin ntau lawm. Cistanche muaj cistanoside, uas yog ib qho antioxidant thiab tuaj yeem txo qhov tsim ntawm cov dawb radicals hauv lub cev, yog li inhibiting melanin ntau lawm.

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Ntsiab lusRosa gallica Cov tawv nqaij laus Flavonoid Antioxidative nyhuv

Taw qhia

Daim tawv nqaij ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv kev tiv thaiv kev phom sij cuam tshuam nrog kev xeeb tub thiab noob caj noob ces, teeb meem ib puag ncig, hloov pauv hormonal, thiab cov txheej txheem metabolic (Mukherjee li al., 2006). Ntawm cov no, ib puag ncig ib puag ncig, xws li raug rau hnub ci ultraviolet (UV) hluav taws xob, ua tus neeg nruab nrab tseem ceeb uas ua rau muaj kev laus ntxov ntxov (Laga thiab Murphy, 2009). Cov tsos mob tseem ceeb ntawm daim tawv nqaij laus, uas tshwm sim los ntawm cov duab-laus, suav nrog kev sib sib zog nqus wrinkles, txawv txav pigmentation, thiab elasticity (Farage li al., 2013; Wlaschek li al., 2001).

Pigmentation yog cov tsos mob ntawm kev laus, uas yog tshwm sim los ntawm qhov txawv txav ntawm melanin, uas ua rau ntau yam kab mob ntawm daim tawv nqaij xws li freckles, melasma, hnub nyoog me ntsis, thiab lwm yam hyperpigmentation syndromes (D'Mello et al., 2016; Seo et al., 2003). Nyob rau hauv txoj kev melanogenesis, tyrosinase yog ib qho tseem ceeb raws li tus nqi txwv enzyme uas hloov L-tyrosine rau L-DOPA thiab oxidizes L-DOPA los ua DOPA-quinone (Akhtar li al., 2015). Yog li ntawd, inhibition ntawm tyrosinase yog txuam nrog melanin synthesis. Ntxiv mus, wrinkle tsim, tshwm sim los ntawm kev poob ntawm collagen fibrils thiab elastase, yog lwm yam ntxwv ntawm photoaging. Matrix metalloproteinase-1 (MMP-1), secreted los ntawm tib neeg daim tawv nqaij fibroblasts, yog lub luag hauj lwm rau collagen degradation thaum lub sij hawm cov duab-aging (Pandel li al., 2013). Kev txo qis ntawm MMP-1 kev qhia, uas inhibits collagen degradation, tuaj yeem txhim kho txoj haujlwm ntawm wrinkles.

Rau cov laj thawj saum toj no, kev siv tyrosinase inhibitors lossis MMP inhibitors yog suav tias yog lub tswv yim zoo rau kev txo qis ntawm daim tawv nqaij laus. Cov kev tshawb fawb yav dhau los tau qhia tias retinol, qej extract (caffeine acid, thiab S-ally cysteine) thiab phytoceramide, kojic acid, arbutin, thiab vitamin C tuaj yeem ua raws li cov tawv nqaij laus inhibitors (Couteau and Coiffard, 2016; Kim et al., 2013) . Txawm li cas los xij, kev siv cov tshuaj no muaj qee yam kev txwv xws li ntau yam kev mob tshwm sim nrog rau cytotoxicity, tsw, thiab xim (Yamakoshi li al., 2003). Yog li ntawd, cov kev tshawb fawb tam sim no tau tsom mus rau kev txhim kho kev nyab xeeb, ib txwm muab los ntawm cov khoom siv uas muaj txiaj ntsig zoo ntawm daim tawv nqaij photoprotection.

Rosa hom, loj hlob thoob ntiaj teb, raug suav hais tias yog qhov zoo ntawm kev noj zaub mov zoo. Rose petal extract (RPE), uas muaj cov ntsiab lus xws li phenolic acid, flavonols, thiab anthocyanins, tau tshaj tawm tias muaj ntau yam txiaj ntsig zoo, xws li kev tiv thaiv daim tawv nqaij, ntxiv rau lwm yam kev ua haujlwm lom neeg (Bitis li al., 2. 017; Lee et al., 2018; Masek et al., 2017; Navarro-Gonzalez et al., 2015). Vim tias cov khoom no ntawm RPE paub tias muaj feem xyuam rau kev laus ntawm daim tawv nqaij, nws tau raug pom zoo los ua tus neeg sib tw muaj peev xwm tiv thaiv tawv nqaij. Txawm li cas los xij, me ntsis paub txog qhov cuam tshuam ntawm RPE ntawm daim tawv nqaij laus. Tsis tas li ntawd, dej thiab ethanol raug suav tias yog cov kuab tshuaj zoo tshaj plaws vim yog qhov sib txawv ntawm qhov sib txawv thiab kev nyab xeeb ntawm kev siv (Abarca-Vargas li al., 2016). Yog li, cov qauv sib txawv ntawm cov dej thiab ethanol (0, 10, 30, 50, 70, 90, thiab 100 feem pua ​​​​ethanol RPE) tau npaj los ntawm kev rho tawm.

Lub hom phiaj ntawm txoj kev tshawb no yog los tshawb xyuas cov txiaj ntsig ntawm RPE rau ntawm daim tawv nqaij dawb thiab txhim kho wrinkle. RPE rho tawm nrog sib txawv extraction hnyav piv (0, 10, 30, 50, 70, 90, thiab 100 feem pua ​​​​ethanol/dej cov kuab tshuaj) tau sim los txiav txim seb qhov zoo tshaj qhov hnyav piv rau extraction.

Cov ntaub ntawv thiab cov txheej txheem

Reagent

Rosa gallica petals tau imported los ntawm Qaib Cov Txwv los ntawm GN Bio (Gyeonggi, Kauslim). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), Penicillin– streptomycin–neomycin, thiab 0.5 feem pua ​​trypsin–EDTA tau yuav los ntawm GIBCO Invitrogen (Auckland, NZ, USA). Cov tshuaj tiv thaiv tshwj xeeb tiv thaiv tyrosinase thiab b-actin tau yuav los ntawm Santa Cruz Biotech (Santa Cruz, CA, USA). Thawj cov tshuaj tiv thaiv ntawm MMP-1 tau txais los ntawm R&D systems. Tag nrho lwm yam tshuaj, suav nrog alpha-melanocyte stimulating hormone (a-MSH), nceb tyrosinase, thiab L-DOPA (L-3,4-dihydroxyphenylalanine) tau yuav los ntawm Sigma-Aldrich Co., LLC (St. Louis, MO, USA).

Kev npaj ua qauv

Rose petals tau tov nrog 100 mL ntawm 0, 10, 30, 50, 70, 90, thiab 100 feem pua ​​(v/v) EtOH (tsis muaj ethanol). Soluble Cheebtsam tau muab rho tawm hauv 80 degree dej siv lub reflux condenser. Cov extract tau lim los ntawm cov ntawv lim xov tooj 2 (Whatman, Maidstone, England), lub tshuab nqus tsev, thiab tom qab ntawd yaj hauv dej distilled thiab khov-qhuav, siv los ua cov qauv rau kev ntsuas kev ua haujlwm.

Cell kab lis kev cai

B16F10 melanoma hlwb tau yuav los ntawm Kauslim Cell Line Bank (Seoul, Kauslim). Tib neeg dermal fibroblast (HDF) hlwb tau txais los ntawm Dr. Jin Ho Chung (College of Medicine, Seoul National University, Seoul, Kauslim). Ob lub hlwb tau coj mus kuaj hauv DMEM ntxiv nrog 10 feem pua ​​​​ntawm fetal bovine serum (v/v) thiab 1 feem pua ​​(v/v) penicillin hauv qab 37 degree, thiab 5 feem pua ​​​​ntawm CO2.

Cell viability

Cell viability raug ntsuas los ntawm MTS [3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-({{7 }}sulfophenyl)-2H-tetrazolium] kev soj ntsuam. Cov noob tau cog rau ntawm 96- cov phiaj zoo thiab loj hlob kom txog thaum sib xyaw. Tom qab ntawd, cov hlwb tau tshaib plab nrog cov tshuaj DMEM tsis pub dhau ib hmos thiab kho nrog cov qauv ntawm qhov qhia tau hais tias muaj zog rau 24 teev. Tom qab raug rau cov qauv, 20 lL ntawm MTS tov tau tso cai rau hnov ​​mob nrog cov hlwb rau 1 h. Absorbance tau ntsuas siv lub microplate nyeem ntawv (Sunrise-Basic Tecan, Tecan Austria GmbH Gro¨dig, Austria) ntawm 570 nm.

Hauv vitro nceb tyrosinase kev ua haujlwm

Qhov kev ntsuam xyuas hauv vitro tyrosinase tau ua raws li cov kev piav qhia yav dhau los (Kim li al., 2015). Hauv luv luv, 40 lL ntawm txhua tus qauv tau ntxiv rau qhov ntsuas tsis tau (20 lL ntawm 0.1 M potassium phosphate), tom qab ntawd incubation rau 30 min nrog 20 lL ntawm nceb tyrosinase (0.02 mg / mL). Tom qab ntawd, 40 lL ntawm substrate (L-DOPA) tau ntxiv rau txhua qhov sib tov. Cov tshuaj tiv thaiv tau tso cai mus rau hauv chav sov li 15 feeb ua ntej tsim cov dopachrome tau soj ntsuam los ntawm kev ntsuas qhov nqus ntawm 475 nm siv tus nyeem ntawv microplate (Infinite 2000 PRO, Tecan, Switzerland).

Kev ntsuam xyuas ntawm melanin ntau lawm

Kev ntsuam xyuas melanin ntau lawm tau ua raws li cov txheej txheem tau piav qhia yav dhau los (Friedmann thiab Gilchrest, 1987; Gordon li al., 1989). B16F10 hlwb (8 9 103 hlwb) tau muab noob rau ntawm 6-zoo daim hlau nrog 2 mL kab lis kev cai xov xwm. Tom qab 24 teev, cov qauv raug pretreated nrog cov hlwb rau 1 h, tom qab uas a-MSH (100 nM) raug rau cov hlwb. Cov hlwb tau sau tom qab 72 h, thiab melanin ntau lawm tau ntsuas los ntawm kev nyeem ntawv microplate (Infinite 2000 PRO, Tecan, Switzerland) ntawm 495 nm.

Western blot

Cov qauv protein tau txais los ntawm cov hlwb, siv 19 Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA). Protein concentration tau kwv yees siv PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific, San Jose', CA, USA). Cov qauv kuaj tau raug thauj mus rau 10 feem pua ​​​​SDS- polyacrylamide gel (Bio-Rad Laboratories, Hercules, California, USA) rau electrophoresis thiab tom qab ntawd xa mus rau Immobilon P membrane (Millipore, Billerica, MA, USA). PVDF daim nyias nyias tau thaiv nrog 5 feem pua ​​​​ntawm cov mis nyuj tsis muaj rog rau 1 h thiab daim nyias nyias tau kho nrog cov tshuaj tiv thaiv tshwj xeeb thaum hmo ntuj ntawm 4 degree. Protein bands tau kuaj pom siv cov khoom siv tshuaj ntsuam xyuas chemiluminescence (GE Healthcare, NJ, USA) tom qab hybridization nrog HRP-conjugated secondary antibody (Cell Signaling).

DPPH radical scavenging assay

DPPH radical scavenging kev ua haujlwm tau ntsuas raws li hauv qab no; {{0}}.2 mL txhua cov extract tau ntxiv rau 3 mL ntawm -ethanol, uas 0.8 mL ntawm 400 lM DPPH yaj hauv ethanol ntxiv. Qhov sib tov no tau vortexed rau 10 s thiab khaws cia ntawm chav tsev kub rau 10 min, thiab absorbance yog ntsuas ntawm 517 nm (Wang li al., 1999). DPPH radical scavenging kev ua si tau qhia raws li feem pua ​​​​ntawm cov absorbance ntawm pab pawg uas tsis muaj DPPH ntxiv. Txhua qhov kev sim tau rov ua dua yam tsawg kawg ntawm 3 zaug.

Tag nrho cov ntsiab lus flavonoid

Tag nrho cov ntsiab lus flavonoid nyob rau hauv cov extracts tau ntsuas siv txoj kev txhuas chloride (Jia li al., 1999) uas tau hloov kho siv catechin. Rau 100 lL ntawm cov extract, 500 lL ntawm distilled dej thiab 30 lL NaNO2 tau ntxiv, tom qab uas 60 lL AlCl3 tau ntxiv 6 min tom qab. Tom qab 5 feeb, 200 lL ntawm 1 M NaOH ntxiv thiab coj mus txog 1 mL nrog dej distilled. Cov tshuaj tau tov zoo thiab centrifuged ntawm 15,928g, ntawm 4 degree, rau 5 min. Tom qab centrifugation, 200 lL ntawm cov supernatant tau txais thiab nqus tau raug ntsuas los ntawm kev nyeem ntawv microplate (Infinite 2000 PRO; Tecan, Switzerland) ntawm 510 nm.

Kev txheeb cais

Kev sim tau ua nyob rau hauv triplicate, thiab cov ntaub ntawv tau qhia raws li qhov nruab nrab ± tus qauv sib txawv (SD). Cov tub ntxhais kawm qhov kev xeem t tau siv rau kev sib piv ib leeg. Kev txheeb xyuas qhov tseem ceeb tau teeb tsa ntawm (#) {{0}} p\ 0.05 thiab (##)=p\ 0.001 rau kev sib piv nrog kev tswj tsis tau; (*)=p\ 0.05 thiab (**)=p \0.001 rau kev sib piv nrog cov pab pawg A-MSH kho.

Cov txiaj ntsig thiab kev sib tham

Extraction ntawm petals ntawm Rosa gallica raws li qhov sib txawv hnyav piv

Yav dhau los RPE tau tsim los ntawm aqueous 70 feem pua ​​(v/v) EtOH extraction (Lee li al., 2018). Txawm li cas los xij, yog tias RPE tau npaj rau lub hom phiaj ntawm daim ntawv thov kev lag luam, cov txheej txheem rho tawm yuav tsum ua kom zoo. Txhawm rau tshawb xyuas cov txiaj ntsig zoo tshaj plaws ntawm RPE rau kev tiv thaiv kev laus ntawm daim tawv nqaij, ntau cov kuab tshuaj tau siv rau RPE (Fig. 1A). Interestingly, cov xim ntawm txhua lub extract txawv, raws li kev soj ntsuam pom (Fig. 1B). Cov xim ntawm EtOH rho tawm, 90 feem pua ​​​​lossis siab dua, yog qhov tseeb, qhov 50 feem pua ​​(v/v) EtOH extract tau tshwm sim los ntawm reddish tshaj plaws. Qhov tshwm sim ntawm colorimeter qhia ib tug * tus nqi rau redness rau 50 feem pua ​​​​EtOH, uas qhia tau hais tias lub siab tshaj plaws ntawm cov extracts (Fig. 1C).

Cov nyhuv ntawm cov extracts ntawm rose petal rau ntawm daim tawv nqaij whitening kev ua si

Txij li tyrosinase yog ib qho tseem ceeb enzyme koom nrog hauv hyperpigmentation ntawm melanin ntau lawm (Chang, 2009), peb tau soj ntsuam cov nyhuv inhibitory ntawm cov extracts ntawm tyrosinase kev ua hauv vitro. Feem ntau cov rho tawm, tshwj tsis yog 100 feem pua ​​​​EtOH, tau nthuav tawm cov koob tshuaj-dependent regression nyob rau hauv vitro tyrosinase kev ua haujlwm (Fig. 2A). Tsis tas li ntawd, 30 feem pua ​​​​thiab 50 feem pua ​​​​ntawm EtOH extracts pom muaj zog inhibitory cuam tshuam dua li ascorbic acid. Ascorbic acid tau piav qhia tias yog cov tshuaj tua kab mob ntawm daim tawv nqaij hauv cov ntaub ntawv dhau los (Chang, 2009). Txhawm rau ntsuas cov tawv nqaij ua kom tawv nqaij dawb ntawm cov khoom rho tawm hauv cov qauv cellular, melanin ntau lawm tau soj ntsuam hauv B16F10 melanoma hlwb tom qab kho nrog txhua cov extract. Ib feem ntawm 100 nM ntawm a-MSH nce melanin ntau lawm thiab txhua qhov extract ntawm Rose Petal inhibited melanin ntau lawm (Fig. 2B). Tsis tas li ntawd, 30 feem pua ​​​​thiab 50 feem pua ​​​​ntawm EtOH rho tawm tau pom qhov muaj zog inhibitory effet ntawm tyrosinase kev ua thiab melanin ntau lawm hauv vitro, feem. Raws li cov xwm txheej zoo sib xws, cov txiaj ntsig ntawm txhua qhov kev rho tawm ntawm tyrosinase qhia tau raug tshuaj xyuas. Tsis tas li ntawd, a-MSH nce tyrosinase qhia thiab 100 lg / mL txhua ntawm cov extracts attenuated tyrosinase qhia (Fig. 2C). Kev kho mob nrog kev rho tawm ntawm 100 lg / mL tsis ua rau cov cell cytotoxicity hauv B16F10 melanoma hlwb (Fig. 2D). Yog li, inhibition ntawm melanin los ntawm RPE yog ntaus nqi los ntawm ob fuction ntawm tyrosinase kev ua si thiab qhia qhov tsis muaj cytotoxicity.

Qhov cuam tshuam ntawm RPE ntawm hnub ci UV (sUV)-induced MMP-1 qhia hauv tib neeg dermal fifibroblasts

Inhibitors ntawm MMP-1 tej zaum yuav raug suav hais tias yog cov tshuaj tiv thaiv wrinkle (Park li al., 2018; Yang et al., 2018). Qhov no yog vim MMP-1 yog ib qho tseem ceeb enzyme, lub luag hauj lwm rau wrinkle tsim thaum lub sij hawm ntawm daim tawv nqaij laus txheej txheem (Pittayapruek li al., 2016). Txhawm rau txiav txim siab los tiv thaiv wrinkle los ntawm RPE, MMP-1 qhia tau raug tshuaj xyuas hauv tib neeg dermal fibroblasts siv Western blot tsom xam. MMP-1 kev qhia tau nce zuj zus los ntawm sUV irradiation (30 kJ / cm2), thiab tag nrho cov extracts inhibited sUV-induced MMP-1 qhia (Fig. 3A). Thaum 100 feem pua ​​​​EtOH rho tawm cov nyhuv me me, 50 feem pua ​​​​EtOH rho tawm tau nthuav tawm cov kev tawm dag zog tshaj plaws ntawm sUV-induced MMP-1 qhia. Tom ntej no, peb tau tshawb xyuas MMP-1 qib ntau lawm hauv kab lis kev cai, vim MMP{19}} yog cov protein uas zais cia rau collagen degradation. Zoo ib yam li qhov uas tau pom hauv daim duab 3A, MMP-1 qib hauv kab lis kev cai tau raug txwv los ntawm txhua qhov rho tawm (Daim duab 3B). Tshwj xeeb, 50 feem pua ​​​​EtOH extract tau pom qhov ua haujlwm siab tshaj plaws ntawm sUV-induced MMP-1 qhia, thiab 100 feem pua ​​​​EtOH extract tau nthuav tawm cov nyhuv me me piv rau lwm cov extracts.

Anti-oxidative kev ua si thiab tag nrho cov ntsiab lus flavonoid ntawm Rose Petal Extracts

Antioxidants uas inhibit ROS ntau lawm paub tias yuav txo tau hyperpigmentation los yog tiv thaiv UV-induced melanin ntau lawm (Yamakoshi li al., 2003). DPPH radical scavenging kev ua haujlwm tau txiav txim siab los sib piv cov kev ua haujlwm antioxidant ntawm cov extracts. Interestingly, txhua lub extract tau pom drastic, koob-dependent, radical scavenging kev ua si. Cov qauv 500 lg / mL tau pom tias muaj kev tiv thaiv oxidative zoo ib yam li cov vitamin C. Ntawm cov extracts, 50 feem pua ​​​​EtOH extract tau pom tias muaj zog tiv thaiv oxidative nyhuv ntawm qhov qis tshaj concentration (50 lg / mL) (Fig. 4A).

Muaj ntau yam sib txawv xws li terpenes, flavonoids, thiab anthocyanins hauv cov paj ntoo (Knapp li al., 1998; Kumar et al., 2008; Oka et al., 1998; Schieber et al., 2005). Cov tshuaj no sawv cev rau ntau yam kev ua ub no (Kumar et al., 2009). Tshwj xeeb, flavonoids tau piav qhia tias yog pawg tseem ceeb ntawm phenolic tebchaw lub luag haujlwm rau cov khoom siv roj ntsha ntawm cov tshuaj tiv thaiv kab mob antioxidant thiab cov tshuaj tiv thaiv kab mob (Du li al., 2016; Jung li al., 2015). Tom qab ntawd, tag nrho cov ntsiab lus flavonoid ntawm txhua qhov extract tau ntsuas. Muaj ntau cov flavonoids nyob rau hauv txhua qhov extract (Fig. 4B). Cov txiaj ntsig tau qhia tias 50 feem pua ​​​​ntawm EtOH extract muaj cov ntsiab lus flavonoid siab tshaj plaws ntawm cov qauv. Cov qauv no tau zoo ib yam li qhov tau pom nyob rau hauv qhov kev ntsuam xyuas los tiv thaiv oxidative.

Cov xim liab tsaus ntawm 50 feem pua ​​​​EtOH hnyav ntawm cov paj ntoo yog xav tias muaj feem xyuam rau anthocyanin, flavonoid (Fig. 1). Qhov txiaj ntsig no ua pov thawj qhov kev tshawb pom ntawm kev tshawb fawb yav dhau los (Oancea li al., 2012). Raws li kev tshawb fawb yav dhau los, anthocyanins hauv blueberry (Vaccinium orymbosum L.) tau zoo tshaj plaws-extracted hauv 50 feem pua ​​​​EtOH hnyav dua li lwm cov kuab tshuaj, suav nrog 60 feem pua, 70 feem pua ​​thiab 80 feem pua ​​EtOH (Oancea li al., 2012). Lawm, anthocyanins tshwm sim raws li glycosides. Yog li peb surmised tias lub polarity ntawm cov anthocyanins yuav tau accorded los ntawm 50 feem pua ​​EtOH hnyav. Lub zog tiv thaiv oxidative kev ua haujlwm ntawm anthocyanins tau pom zoo nyob rau hauv ntau yam qauv (Kalt li al., 2003; RiceEvans li al., 1995). Vim tias 50 feem pua ​​​​ntawm EtOH Rose Petal Extract muaj cov ntsiab lus anthocyanin siab tshaj plaws, cov tshuaj tiv thaiv oxidative ntawm 50 feem pua ​​EtOH extract yog qhov muaj zog tshaj ntawm 50 lg / mL extracts.

Hauv cov ntsiab lus, peb txoj kev tshawb fawb pom tau tias muaj peev xwm ntawm RPE ua cov khoom tiv thaiv kev laus ntawm daim tawv nqaij. Rau lub hom phiaj ntawm kev lag luam daim ntawv thov, optimization ntawm extraction tej yam kev mob yog qhov tseem ceeb nyob rau hauv thiaj li yuav txo tau cov nqi ntau lawm. Txoj kev tshawb fawb tam sim no tau tshawb xyuas cov kuab tshuaj zoo tshaj plaws rau Rose petal extraction, nrog rau kev siv los tiv thaiv kev laus ntawm daim tawv nqaij. Cov flavonoids hauv cov paj ntoo tau muab rho tawm zoo tshaj plaws los ntawm 50 feem pua ​​EtOH hnyav. Cov extract no tau pom qhov muaj zog tshaj plaws los tiv thaiv oxidative kev ua si nrog rau kev tiv thaiv ntawm daim tawv nqaij laus xws li tawv nqaij dawb thiab wrinkle suppression kev ua. Cov kev tshawb fawb soj ntsuam thiab kev txheeb xyuas kev ruaj ntseg yuav tsum tau kuaj xyuas nws qhov kev tsim nyog rau kev lag luam.

Kev lees paubQhov kev tshawb fawb no tau txais kev txhawb nqa los ntawm Kev Tshawb Fawb Kev Tshawb Fawb (E0183112-02) ntawm Lub Tsev Haujlwm Saib Xyuas Khoom Noj Hauv Kaus Lim Kauslim (KFRI) tau nyiaj los ntawm Ministry of Science, ICT & Future Planning thiab los ntawm Kaus Lim Kauslim Lub Tsev Haujlwm Saib Xyuas Kev Npaj thiab Kev Ntsuam Xyuas Technology hauv Khoom Noj, Kev Ua Liaj Ua Teb , Forestry thiab Fisheries (IPET) los ntawm High Value-added Food Technology Development Program, tau nyiaj los ntawm Ministry of Agriculture, Food and Rural Affairs (MAFRA) (118060-03-2- HD020).

Ua raws li cov qauv kev coj ncaj ncees

Cov ntaub ntawv

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