Abstract

Lub Hom Phiaj: Tshawb nrhiav lub peev xwm mechanism of Cistanche tag nrho glycosidesntawminflammatory plab hnyuv kab mob(IBD) los ntawm lub network pharmacology thiab kev tshawb fawb tsiaj. Txoj Kev: Pubchem cov ntaub ntawv thiab cov ntaub ntawv siv los sau cov qauv peb sab ntawm xya lub ntsiab lus tseem ceeb hauv Cistanche tag nrho glycosides. Tau txais cov ntaub ntawv ntawm cov khoom muaj feem cuam tshuam txog cov hom phiaj thiab IBD-txog noob hom phiaj los ntawm cov ntaub ntawv xws li PharmMapper, UniProt, thiab GeneCards. Tom qab ntawd kos ib daim duab Venn ntawm lub hom phiaj tseem ceeb ntawm Cistanche thiab cov hom phiaj ntawm IBD kom tau txais cov hom phiaj kev sib tshuam, thiab xa cov phiaj xwm kev sib tshuam mus rau String database rau cov protein-protein interaction (PPI) kev tshuaj ntsuam xyuas. Siv DAVID database los sib piv cov khoom xyaw nquag ntawm Cistanche tag nrho glycosides hauv IBD

Cov hom phiaj uas tiv thaiv kev tiv thaiv tau txheeb xyuas los ntawmGene Ontology (GO)Functional enrichment thiab Kyoto Encyclopedia of Genes and Genomes (KEGG) txoj kev txhawb nqa. Cytoscape3.8.Cistanche. Lub hom phiaj thiab txoj hauv kev ntawm glycosides hauv kev kho IBD, thiab tus qauv IBD nas tau tsim los rau kev txheeb xyuas ntxiv. Cov txiaj ntsig: Muaj 254 lub hom phiaj ntawm tag nrho cov khoom xyaw nquag ntawm Cistanche uas muaj kev tiv thaiv ntawm IBD, thiab 30 lub hom phiaj tseem ceeb raug tshuaj xyuas los ntawm PPI tsom xam. GO functional enrichment thiab KEGG pathway enrichment pom tias Cistanche tag nrho glycosides tuaj yeem tswj cov kab mob qog noj ntshav, mTOR txoj hauv kev, TGF- txoj hauv kev, JAK-STAT txoj hauv kev, AMPK txoj hauv kev, thiab lwm yam, yog li cuam tshuam rau lub xov tooj ntawm tes taw qhia, kev loj hlob, kev sib txawv, thiab apoptosis. Ua lub luag haujlwm. Cov txiaj ntsig ntawm kev sim tsiaj pom tau hais tias Cistanche tag nrho glycosides tuaj yeem txo qhov poob phaus thiab fecal los ntshav hauv IBD nas, txo cov kab mob kev ua ub no, thiab muaj txiaj ntsig inhibit qhov kev qhia ntawm ob lub hom phiaj proteins nyob rau hauv tus po, mTOR thiab TGF-. Los ntawm sequencing 16S rDNA amplicon ntawm nas quav rau kev ua haujlwm annotation ntawm PICRUST2 gene, kev tshuaj xyuas pom tias Cistanche tag nrho glycosides tswj IBD kab mob thiab cell phab ntsa / cell membrane / hnab ntawv biogenesis, lipid metabolism, qab zib metabolism thiab cuam tshuam txog kev tiv thaiv teeb liab transduction hauv nas. . Muaj kev sib raug zoo nrog cov txheej txheem kev taw qhia, uas zoo ib yam nrog cov txiaj ntsig ntawm lub network pharmacology. Xaus: Cistanche tag nrho glycosides tuaj yeem dhau los ntawm ntau yam khoom thiab ntau lub hom phiaj

Cov ntsiab lus thiab ntau txoj hauv kev tuaj yeem tiv thaiv IBD tau zoo, uas muab cov txheej txheem tshiab thiab cov tswv yim tshiab rau kev nthuav tawm cov ntawv kho mob ntawm Cistanche hauv kev kho IBD. Nws cov txheej txheem tshwj xeeb thiab cov khoom siv hauv paus yuav tsum tau txheeb xyuas los ntawm kev tshawb fawb tob tob.

Ntsiab lus:Cistanche tag nrho glycosides; network pharmacology;mob;

Nyem qhov no kom tau txais Natural organic CISTANCHE EXTRACT nrog 30% ECHINACOSIDE thiab 12% ACTEOSIDE rauINFLAMMATORY BOWEL DISEASE(IBD)

Inflammatory bowel disease (IBD) yog ib qho tshwj xeebmob plab hnyuv inflammatory kab mobuas tsis paub qhov laj thawj, suav nrog ob hom kab mob: Crohn's Disease (CD) thiab ulcerative colitis (Ulcerative Colitis, UC) [1]. Raws li ntawm 2017, 6.8 lab tus tib neeg thoob ntiaj teb tau kuaj pom tias muaj IBD, thiab qhov xwm txheej thoob ntiaj teb thiab kev kis tau nce zuj zus txhua xyoo. Feem ntau ntawm IBD yog siab dua hauv North America thiab Western Europe [2-3]. Los ntawm 1990 txog 2019, qhov xwm txheej ntawm IBD hauv Suav teb tau nce los ntawm 1.45/100,{10}} mus rau 3.62/100,{14}}, nrog rau tag nrho nce ntawm 149.66%; tus neeg tuag tau txo los ntawm 0.47/100,{20}} mus rau 0.33/100,{24}}, nrog rau tag nrho cov kev txo qis ntawm 29.79%[4] . Raws li Tuam Tshoj cov pej xeem muaj hnub nyoog, kev nce me me ntawm qhov tshwm sim siab ntawm cov neeg mob laus yuav ua rau lub nra hnyav rau kev kho mob thiab kev noj qab haus huv.

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Cov kev tshawb fawb tau ua pov thawj tias kev tswj hwm kev noj haus tuaj yeem pab txo cov tsos mob xws li mob plab lossis raws plab hauv cov neeg mob IBD thiab txo nws cov teeb meem; Nws kuj tseem tuaj yeem tswj cov kev muaj pes tsawg leeg thiab kev ua haujlwm ntawm cov hnyuv microorganisms, cuam tshuam rau txoj hnyuv homeostasis, thiab tswj cov hnyuv ua haujlwm zoo [5]. Cistanche, ua zaub mov thiab tshuaj cog nrog keeb kwm ntev, yog siv dav hauv kev kho mob plab hnyuv hauv cov tshuaj suav tshuaj. Nws kuj tseem suav tias muaj ntau yam teebmeem kev noj qab haus huv, yog li nws tau nyiam ntau yam hauv kev noj qab haus huv zaub mov [6-7]. Xyoo 2020, Cistanche ( suab puam) tau teev tseg raws li cov khoom noj thiab tshuaj homologous, thiab kev tsim khoom thiab kev ua haujlwm tau pib [6-7]. Ua zaub mov, Cistanche tuaj yeem tiv thaiv IBD hauv lub neej txhua hnub; Raws li cov khoom siv tshuaj, nws tuaj yeem txo cov tsos mob thaum kho IBD thiab muaj qee yam kev lag luam thiab tshuaj muaj nqis.

Qee cov kws tshawb fawb tau pom tias suab puam Cistanche aqueous extract tuaj yeem ua rau lub cev tsis muaj zog, tswj kev tiv thaiv kab mob, thiab tiv thaiv kab mob plab hnyuv [8]. Jia et al. [9] pom tias Echinacoside (ECH) muab rho tawm los ntawm Cistanche tuaj yeem txhim kho DSS-induced colitis hauv nas; ECH tuaj yeem tiv thaiv kev tuag ntawm tes thiab txhim kho mucosa los ntawm stimulating plab hnyuv epithelial cell proliferation thiab upregulating TGF-. Kev kho cov ntaub so ntswg [10]. ECH kuj tseem tuaj yeem txo qis LPS-vim apoptosis thiab o ntawm cov kab mob hauv plab hnyuv epithelial los ntawm inhibiting theem qhia ntawm mTOR / STAT3 txoj hauv kev [11]. Tsis tas li ntawd, verbascoside tuaj yeem ua raws li cov dawb radical scavenger hauv lub cev, ua kom qeeb ntawm kev loj hlob ntawm 2, 4-Dinitrobenzene sulfonic acid (DNBS)-induced colitis hauv nas thiab txo cov nqaij mos puas [12].

CISTANCHE SUPPLEMENT CONSTIPATION RELIEF SUPPLEMENTS

Network pharmacology yog ib qho kev kawm sib koom ua ke uas koom nrog bioinformatics, biochemistry, pharmacology thiab kev suav lej. Nws siv cov ntaub ntawv loj, bioinformatics thiab suav cov cuab yeej los tshuaj xyuas thiab simulate complex tshuaj metabolism hauv cov kab mob. Kev twv ua ntej ntawm cov txheej txheem ntawm kev ua thiab kev siv tshuaj muaj txiaj ntsig muab kev txhawb nqa tseem ceeb rau kev txhim kho cov tshuaj tshiab thiab kev ua kom zoo ntawm cov tshuaj uas twb muaj lawm [13-14]. Lub hauv paus ntawm lub network pharmacology yog tsim kom muaj cov qauv sib txuas ntawm cov tshuaj thiab cov molecules hauv cov kab mob los qhia kev sib cuam tshuam ntawm cov tshuaj thiab cov hom phiaj cov proteins, metabolic pathways, cell signaling pathways, thiab lwm yam. [15]. Nrog rau kev nce qib ntawm kev tshawb fawb thiab thev naus laus zis tsis tu ncua, network pharmacology yuav txuas ntxiv ua lub luag haujlwm tseem ceeb hauv kev kho tshuaj thiab pab txhawb kev txhim kho cov neeg mob lub neej thiab kev noj qab haus huv.

Cov kev tshawb fawb tau pom tias Cistanche tag nrho glycosides tuaj yeem cuam tshuam cov lus teb inflammatory ntawm BV2 hlwb raug ntxias los ntawm LPS [16], inhibit kev loj hlob ntawm daim siab mob cancer [17], tiv thaiv daim siab [18], txhim kho kev nco thiab kev paub [19-20 ], thiab tiv thaiv kev laus [21] thiab lwm yam haujlwm. Tam sim no, muaj qee qhov kev tshawb fawb qhia txog cov txiaj ntsig ntawm Cistanche tag nrho glycosides ntawm kab mob plab hnyuv. Txoj kev tshawb no tshawb txog cov txheej txheem ntawm kev ua ntawm Cistanche tag nrho glycosides ntawm cov kab mob plab hnyuv inflammatory raws li kev siv tshuaj hauv network thiab kev sim tsiaj, thiab muab cov tswv yim tshiab thiab cov tswv yim rau nws daim ntawv thov kho mob hauvkev kho mob ntawm IBD.

1 Cov khoom siv thiab cov cuab yeej siv

1.1 Reagents

Dextran sodium sulfate (DSS, txheeb ze molecular hnyav 36 000 ~ 50 000), American MP Company; Cov khoom siv kuaj cov zis thiab quav occult, Nanjing Jiancheng Bioengineering Institute; Tsis muaj ethanol, Sinopharm Chemical Reagent Co., Ltd.; xylene, Shanghai Ling Feng Chemical Reagent Co., Ltd.; PBS tsis, RNA extraction tov, thawj-strand thim rov qab cov khoom siv, 2 × real-time quantitative PCR amplification premix tov, RTQPCR primers, Wuhan Sevier Biotechnology Co., Ltd.; sterile thiab tsis muaj enzyme dej,

HyClone USA.

CISTANCHE SUPPLEMENT CONSTIPATION RELIEF SUPPLEMENTS

1.2 Cov cuab yeej tseem ceeb thiab khoom siv

KZ-III-FP low-temperature grinder, Wuhan Sevier Biotechnology Co., Ltd.; CT15RE high-speed centrifuge, HITACHI tuam txhab ntawm Nyiv; CFX fluorescence quantitative PCR instrument, Bio-rad Company ntawm Tebchaws Meskas; SW-CJ-1FD ultra-clean workbench, Suzhou Antai Cua Technology Co., Ltd.; NanoDrop2000 ultra-trace spectrophotometer, Thermo Company, Lub teb chaws Yelemees; FBZ2001-up-p txheem reagent hom ntshiab dej ntsuas, Qingdao Fulham Technology Co., Ltd.; INFINITE MNANO multi-function microplate nyeem ntawv, TECAN, Switzerland tuam txhab, UV1600 UV spectrophotometer, Shanghai Miputa Instrument Co., Ltd.

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