Kev Ntsuam Xyuas Ntawm Cov Txheej Txheem Sib Txawv Ntawm Cov Txheej Txheem Qhuav Thiab Cov Excipients Ntawm Hygroscopicity Ntawm Scutellariae Radix Thiab Coptidis Rhizoma Extracts Raws Li Lub Cev Tus ntiv tes Spectrum
Sep 02, 2024
Abstract:Lub Hom Phiaj Noj Huangqin (Scutellariae Radix, SR) thiabHuanglian (Coptidis Rhizoma, CR) extract hmoovraws li cov khoom tshawb nrhiav, qhov cuam tshuam ntawm ntau txoj kev ziab kom qhuav thiab cov ntaub ntawv pabcuam ntawm kev nqus dej tau raug tshawb xyuas.
Txoj kev Cov hmoov extract ntawm SR thiab CR tau npaj los ntawmKev ziab kom qhuav (CD), Vacuum drying (VD), thiabtshuaj tsuag kom qhuav (SD). Kaum ob indexes, xws linoo noo cov ntsiab lus,noo noo nqus tus nqi,thaj chaw tshwj xeeb, nruab nrab diam, span, dav, Hausnerratio, lub kaum sab xis ntawm repose, bulk density, tapped density, gap rate, thiab Carr index, tau siv los tsim kom muaj lub cev ntiv tes ntawm extractpowder. Qhov thib ob indexes tau hloov mus rau hauv tsib yam tseem ceeb: uniformity, pileup, compressibility, fluidity, thiab stability. Tom qab ntawd cov txheej txheem ziab kom zoo tshaj plaws raug xaiv. Thawj qhov kev tsom xam (PCA) thiab ib feem tsawg squares tsom (PLS) tau siv los txheeb xyuas qhov sib txheeb ntawm lub cev tsis zoo thiab tus cwj pwm nqus dej noo. Cov teebmeem ntawm tsib excipients, suav nrog dextrin, -cyclodextrin, pre-gelatinized starch, anhydrous lactose, thiab microcrystalline cellulose, ntawm cov dej noo sorption ntawm cov hmoov tau optimized. Lub dynamic nkhaus ntawm lub sij hawm noo noo sorption ntawm cov hmoov tau kos, thiab lub sij hawm semi-equilibrium ya raws sorption lub sij hawm (t1/2) thiab equilibrium ya raws sorption lub sij hawm (F∞) tau muab los tsim ib tug ob-seem kev ntsuam xyuas Performance index los ntsuam xyuas dynamic ya raws. sorption tus cwj pwm ntawm cov hmoov thiab tshuaj xyuas qhov zoo tshaj plaws noo noo-pov thawj excipients. Cov txiaj ntsig Raws li kev tshawb fawb ntawm lub cev ntiv tes zoo sib xws, tau txais cov txiaj ntsig hauv qab no: SR: Qhov zoo sib xws ntawm cov tshuaj tsuag kom qhuav extract hmoov (SRSD) yog 0.98, 0.99, 0. 99, thiab qhov zoo sib xws ntawm lub tshuab nqus tsev ziab extract hmoov (SR-VD) yog 0.87, 0.92, 0.89, uas yog qhov zoo dua li ntawm huab cua siab kom qhuav. Qhov zoo sib xws ntawm lub tshuab nqus tsev ziab, tshuaj tsuag kom qhuav, thiab atmospheric siab ziab yog {{20}}.95, thiab 0.69, raws li. CR: Tsis muaj qhov sib txawv tseem ceeb ntawm peb txoj kev ziab. Qhov zoo sib xws ntawm cov tshuaj tsuag kom qhuav extractpowder (CR-SD) yog 0.99, 1.00, thiab 0.99, thiab qhov zoo sib xws ntawm lub tshuab nqus tsev ziab, tshuaj tsuag kom qhuav, thiab atmospheric siab ziab yog 0.94 thiab 0.61, raws li. Raws li kev ntsuam xyuas dav dav ntawm 5 qhov ntsuas thawj theem, nws tuaj yeem xaus lus tias lub tshuab nqus tsev thiab cov tshuaj tsuag ziab kom zoo dua. Los ntawm daim ntawv qhia matrix tshav kub, PCA thiab PLS, ncua, dav, SSA thiab D50 muaj feem cuam tshuam rau H, ua raws li Dc thiab Da (P <0.05). Los ntawm dynamic two-dimensional characterization technology, nws tuaj yeem pom tias cov khoom siv tuaj yeem txhim kho cov dej noo ntawm cov hmoov. Cov kev tshawb fawb qhia tau hais tias: SR: Raws li peb txoj kev ziab ziab ntawm cov hmoov extract, cov qauv yog nyob rau hauv chav kawm thib ob, nrog rau qhov sib npaug loj ntawm cov dej noo nqus thiab qhov nqus dej qeeb, thiab cov tshuaj sib xyaw ua ke yog nyob rau hauv chav kawm thib peb, nrog me me. equilibrium noo noo absorptioncapacity thiab qeeb noo noo nqus tus nqi. Ntawm lawv, qhov sib npaug ntawm noo noo nqus ntawm atmospheric qhuav qauv ntawm SRand cov tshuaj-kev sib xyaw ntawm -cyclodextrin yog qhov tsawg tshaj plaws, nrog F∞=9.12%. Nqus-qhuav cov qauv ntawm SR thiab cov tshuaj adjuvant sib tov ntawm -cyclodextrin muaj qhov tsawg tshaj plaws noo noo nqus tus nqi, t1/2=69.33 h, CR: Lub atmospheric siab thiab sprayextract hmoov belongs rau chav kawm thib ob, thiab cov tshuaj- auxiliary sib tov belongs rau chav kawm thib peb. Lub equilibrium moistureabsorption ntawm cov tshuaj-auxiliary sib tov ntawm CR lub tshuab nqus tsev qauv thiab lactose yog qhov tsawg tshaj plaws, nrog F∞=7.72%, thiab cov dej noo nqus ntawm cov tshuaj-auxiliary sib tov ntawm CR nqus qauv thiab -cyclodextrin yog qhov tsawg tshaj plaws, t1/2=76.33 h.
Xaus Txhim kho thedrying txoj kev ntawm tsoos suav tshuaj rho tawm thiab xaivhigh-zoo noo noo-pov thawj excipientstuaj yeem txhim kho cov moisturption ntawm Suav tshuaj extracts.
Ntsiab lus:rho tawm;Coptidis Rhizoma; Scutellariae Radix; lub cev ntiv tes; txoj kev kom qhuav; excipients; tseem ceeb componentanalysis; kev tsom xam ib feem tsawg squares; dynamic two-dimensional characterization technology; noo noo nqus; kev ziab khaub ncaws; lub tshuab nqus tsev ziab; tshuaj tsuag kom qhuav
TSHIAB DRYING METHOD HERB EXTRACT-High QUALITY CISTANCHE
Suav tshuaj Extracts (CMEs)yog tau los ntawm Suav cov ntaub ntawv tshuaj los yog suav tshuaj tebchaw los ntawm extraction, sib cais, concentration, thiab ziab. Lawv muaj qhov ua tau zoo meej thiab yog cov neeg nruab nrab tseem ceeb hauv cov txheej txheem tsim khoom ntawm Suav tshuaj npaj. Lawv feem ntau yog siv los npaj cov khoom npaj xws li suav tshuaj granules, ntsiav tshuaj, thiab tshuaj ntsiav [1]. Suav tshuaj extracts yog complex nyob rau hauv muaj pes tsawg leeg thiab muaj ntau yam hygroscopic Cheebtsam, xws li qab zib, starch, protein, thiab tannin. Lawv yooj yim heev los nqus dej noo thaum sib cuag nrog huab cua, ua rau muaj viscosity thiab nyuaj hauv kev npaj molding. Lawv kuj yog cov hygroscopic heev tom qab tau ua rau hauv cov khoom npaj. Thaum lub sij hawm cia, lawv yuav ua rau muaj teeb meem kev ruaj ntseg xws li agglomeration ntawm granules thiab capsules, thiab darkening ntawm ntsiav tshuaj xim.
Kev kho cov tshuaj suav tshuaj tuaj yeem txo qhov hygroscopicity ntawm extract hmoov, tab sis cov khoom xyaw zoo xws li flavonoids, glycosides, thiab organic acids kuj yuav poob mus rau qhov sib txawv, uas tsis ua raws li cov txheej txheem tsim khoom tiag tiag. Xaiv txoj kev ziab kom tsim nyog thiab cov txheej txheem tsim nyog muaj txiaj ntsig zoo rau lub cev ntawm cov tshuaj suav tshuaj extract hmoov, uas tuaj yeem ua rau cov hmoov me me, txo qhov tshwj xeeb ntawm thaj chaw, thiab txo qhov nqus noo noo ntawm cov hmoov extract. [2-5]. Ntxiv cov excipients tsim nyog rau Suav tshuaj extract hmoov tsis tsuas yog txo hygroscopicity thiab txhim kho nws stability, tab sis kuj ua raws li ib tug diluent, nplaum, los yog lubricant, uas yog zoo rau kev npaj molding [6-8]. Kev siv cov hmoov av hloov tshuab, cov txheej txheem dej noo-pov thawj, thiab lwm yam tuaj yeem txo cov hygroscopicity ntawm cov khoom, thiab ntau txoj hauv kev kuj tuaj yeem siv ua ke los txhim kho cov dej noo-pov thawj. Hauv txoj kev tshawb no, cov extracts ntawm Scutellaria baicalensis thiab Coptis chinensis, uas feem ntau siv cov tshuaj suav tshuaj nrog muaj zog hygroscopicity, raug xaiv los ua cov khoom tshawb fawb. Tag nrho ntawm 12 hmoov kev tshawb fawb ntsuas, suav nrog cov ntsiab lus dej, qhov nqus dej noo, thaj chaw tshwj xeeb, qhov nruab nrab txoj kab uas hla, particle loj faib dav, qhov loj me me, Hausner piv, lub kaum sab xis ntawm repose, bulk ntom, kais ntom, interstitial ratio, thiab Carr index, tau txiav txim. Lub cev ntiv tes tau tsim, thiab kev tsom xam cov ntsiab lus tseem ceeb (PCA) thiab ib feem ntawm qhov ntsuas qhov tsawg kawg nkaus (PLS) tau ua rau ntawm txhua qhov kev ntsuas lub cev los tshawb txog qhov sib txheeb ntawm txhua qhov kev ntsuas lub cev thiab tus cwj pwm hygroscopic. Cov teebmeem ntawm cov txheej txheem ziab qhuav sib txawv thiab cov khoom pov thawj noo noo ntawm lawv cov hygroscopicity tau ua kom zoo dua los muab cov ntaub ntawv siv rau kev tsim cov khoom siv pov thawj noo noo rau cov tshuaj suav tshuaj.
1 Cov cuab yeej thiab cov khoom siv
1.1 Cov cuab yeej
Bettersize 2600 laser particle loj faib analyzer, Dandong Better Instrument Co., Ltd.; HZ-D (III) lub tshuab nqus tsev twj tso kua mis, Zunyi Sihua Instrument Co., Ltd.; DHG-9140Ib qhov hluav taws xob qhov kub thiab txias tshuab ziab khaub ncaws, Shanghai Jinghong Experimental Equipment Co., Ltd.; LANYI-6000Y tshuab ziab khaub ncaws, Shanghai Lanyi Industrial Co., Ltd.; D2F-6021 lub tshuab nqus tsev vacuum, Shanghai Yiheng Scientific Instrument Co., Ltd.; XL-10B high-speed Suav tshuaj pulverizer, Guangzhou Xulang Machinery Equipment Co., Ltd.
1.2 Cov khoom siv
Scutellaria baicalensis, batch tooj 210527, Jiangnantong Mitsukoshi Suav Tshuaj Pieces Co., Ltd.; Coptis chinensis, batch naj npawb 211027, Jiangsu Chengkai suav tshuaj Co., Ltd.; Cov ntawv tau txheeb xyuas los ntawm xibfwb Chen Jianwei ntawm Nanjing University of suav tshuaj, thiab lawv yog cov cag qhuav ntawm Scutellaria baicalensis Georgi, cov nroj tsuag ntawm genus Scutellaria hauv tsev neeg Lamiaceae, thiab cov rhizomes qhuav ntawm Coptis chinensis Franch. lub genus Coptis nyob rau hauv tsev neeg Ranunculaceae, ob qho tib si uas ua tau raws li cov qauv ntawm 2020 tsab ntawm Suav Pharmacopoeia; 95% ethanol (batch tooj 20220712), lactose (batch tooj 20220527), -cyclodextrin (batch tooj 20201118), dextrin (batch tooj 20210309), Sinopharm Chemical Reagent Co., Ltd.; microcrystalline cellulose, batch tooj C2205104, Shanghai Shenmei Pharmaceutical Development Technology Co., Ltd.; Pregelatinized starch, batch tooj Z22J8W40375, Shanghai Yuanye Biotechnology Co., Ltd.
2 Cov txheej txheem thiab cov txiaj ntsig
2.1 Kev npaj ntawm extract hmoov ntawm Suav tshuaj ntsuab tshuaj extract
2.1.1 Kev npaj ntawm extract
(1) Scutellaria baicalensis (SR): Noj 300 g ntawm Scutellaria baicalensis slices, ntxiv 10 npaug ntawm cov dej thiab boil rau 3 zaug, muab cov decoctions, mloog zoo rau ib tug tsim nyog tus nqi, ntxiv 70% ethanol rau precipitate, do tusyees, cia sawv ntsug, coj tus supernatant kom rov qab tau ethanol, thiab mloog zoo rau ib qho ntshiab muab tshuaj txhuam nrog tus txheeb ze ntom ntom ntawm 1.1 thiab 1.3 rau kev siv.
(2) Coptis chinensis (CR): Siv 300 g ntawm Coptis chinensis slices, ntxiv 12 npaug ntawm cov dej, thiab boil rau 2 zaug, muab cov decoctions, mloog zoo rau ib tug tsim nyog tus nqi, thiab mloog zoo rau ib tug ntshiab muab tshuaj txhuam nrog ib tug txheeb ze. Qhov ceev ntawm 1.1 thiab 1.3 rau kev siv.
2.1.2 Kev npaj cov hmoov extract los ntawm ntau txoj kev ziab
(1) Kev ziab kom qhuav (CD) cov hmoov nplej qhuav: ziab cov hmoov nplej nrog cov txheeb ze ntawm 1.3 ntawm 75 degree nyob rau hauv lub siab ib txwm, crushed nrog high-speed suav tshuaj grinder rau 90 vib nas this tom qab ziab, thiab dhau los ntawm ib tug 80-mesh sieve kom tau cov hmoov extract.
(2) Nqus kom qhuav (VD) extract hmoov: Qhuav lub tuab muab tshuaj txhuam nrog ib tug txheeb ze ntom ntawm li 1.3 ntawm 75 degree nyob rau hauv lub nqus tsev vacuum, zuaj nws nrog ib tug high-ceev tshuaj suav tshuaj grinder rau 90 vib nas this tom qab ziab, thiab dhau los ntawm ib tug { {6}}mesh sieve kom tau cov hmoov extract.
(3) Txau kom qhuav (SD) extract hmoov: Txau kom qhuav cov hmoov nplej ntshiab nrog cov txheeb ze ntom ntom ntawm 1.1, thiab dhau los ntawm 80-mesh sieve kom tau txais cov hmoov extract.
2.2 Kev tsim kho ntawm lub cev ntiv tes
2.2.1 Kev txiav txim siab ntawm lub cev ntsuas ntawm cov hmoov extract
Lub stability, uniformity, fluidity, stacking thiab compressibility ntawm extract hmoov yog coj raws li lub hauv paus ntsiab lus ntawm lub cev ntiv tes, thiab cov ntsiab lus ntawm noo noo (HR), noo noo nqus tus nqi (H), tshwj xeeb deg cheeb tsam (SSA), nruab nrab txoj kab uas hla ( D50), particle loj faib dav (span), particle loj ntau (dav), Hausner piv (IH), lub kaum sab xis ntawm repose ( ), bulk ntom (Da), tapped ceev (Dc), qhov sib txawv (Ie), Carr index (IC) thiab tag nrho ntawm 12 qhov ntsuas yog qhov ntsuas thib ob [9-12].
2.2.2 Kev ntsuas kev ntsuas thiab cov qauv kev suav rau txhua qhov ntsuas nruab nrab ntawm cov hmoov extract
(1) : Txiav txim siab los ntawm txoj kev funnel ruaj. Txhim kho lub funnel ntawm ib qho siab tshaj ntawm daim ntawv sib koom ua ke, ntxiv cov ntaub ntawv los ntawm lub funnel mus txog rau sab saum toj ntawm lub khob hliav qab tsuas yog nyob rau hauv kev sib cuag nrog hauv qab ntawm lub funnel, ntsuas lub khob hliav qab txoj kab uas hla, coj qhov ratio ntawm lub funnel hauv qab qhov siab (h ) mus rau lub cone vojvoog (r) raws li tus nqi tangent, xam lub kaum sab xis ntawm repose, thiab coj tus nqi nruab nrab tom qab peb qhov kev ntsuas sib npaug. Cov mis laij lej tau qhia hauv cov mis (1).
tan ± h/r (1)
(2) Da: Xaiv lub thawv nrog lub ntim ruaj khov (V), thiab cia cov hmoov ntws mus rau hauv lub thawv ntim tom qab vibration thiab sieving. Tom qab cov hmoov ntim rau hauv lub thawv, siv lub scraper los txhuam cov hmoov ntau dhau, thiab ntsuas qhov loj ntawm lub thawv ua ntej thiab tom qab ntxiv cov qauv (m0 thiab m1), raws li. Cov mis laij lej yog qhia nyob rau hauv formula (2).
Da＝(m1－m0)/V (2)
(3) Dc: Txoj kev txiav txim siab yog txoj kev ntim ntim ruaj khov. Xaiv ib lub thawv nrog lub ntim ruaj khov (V), ntsuas lub khob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob Tom qab vibrating, ntsuas tag nrho qhov loj ntawm lub thawv thiab hmoov (m). Yog tias qhov sib txawv ntawm qhov sib txawv ntawm 200 thiab 400 zaus ntawm kev vibration tsis ntau dua 2%, xam Dc raws li cov qauv (3). Yog tias qhov sib txawv ntawm qhov sib txawv ntawm 200 thiab 400 zaus ntawm kev vibration ntau dua 2%, txuas ntxiv vibrate rau 100 zaug kom txog rau thaum qhov sib txawv ntawm ob qhov kev ntsuas sib law liag yog tsawg dua 2%. Tshem tawm cov qauv ntau dhau, ntsuas qhov loj ntawm lub khob ntsuas ntim nrog cov qauv (m1), thiab ntsuas peb zaug ua ke. Cov mis laij lej tau qhia hauv cov mis (3).
Dc＝(m－m0)/V (3)
(4)Ie: Xam los ntawm Da thiab Dc, cov qauv kev suav yog qhia hauv mis (4). Ie＝(Dc－Da)/DaDc (4)
(5) IC: xam los ntawm Da thiab Dc, cov qauv kev suav yog qhia hauv mis (5). IC＝(Dc－Da)/Dc (5)
(6) IH: Xam los ntawm Da thiab Dc, cov qauv kev suav yog qhia hauv mis (6).
IH＝Dc/Da (6)
(7) HR: Raws li 2020 tsab ntawm Suav Pharmacopoeia [13], 1 g ntawm cov qauv hmoov yog raug hnyav thiab sib npaug sib npaug rau hauv lub raj mis hnyav (m2) nrog qhov hnyav tas li, nrog rau tag nrho qhov hnyav ntawm m3 thiab tuab. tsis ntau tshaj 5 mm. Lub raj mis qhib thiab qhuav ntawm 100-105 degree rau 5 h. Lub hau lub raj mis kaw thiab muab tso rau hauv lub tshuab ziab khaub ncaws. Nws yog txias rau 30 min thiab raug hnyav. Tom qab ntawd nws yog qhuav ntawm qhov kub siab saum toj no rau 1 h, txias, thiab hnyav kom txog thaum qhov sib txawv ntawm ob qhov sib law liag tsis pub tshaj 5 mg. Qhov hnyav tas li yog m4. Cov dej ntsiab lus ntawm cov qauv yog xam raws li qhov poob loj. Peb cov qauv yog ntsuas nyob rau hauv parallel thiab cov ntsiab lus dej yog xam. Cov mis laij lej tau qhia hauv cov mis (7).
HR ＝(m3－m4)/(m3－m2) (7)
(8) H: Nqa lub raj mis hnyav, qhuav nws, muab tso rau hauv lub tshuab desiccator uas muaj cov tshuaj sodium chloride saturated rau 24 teev, thiab ntsuas nws qhov hnyav (m5). Noj li ntawm 1 g ntawm hmoov kis nws tusyees nyob rau hauv lub cev hnyav, thiab ua kom qhov hnyav tag nrho (m6). Tshem cov fwj uas hnyav thiab muab tso rau hauv lub tshuab desiccator rau 24 teev, npog lawv nrog lub kaus mom, thiab tom qab ntawd tshem tawm thiab ntsuas qhov hnyav (m7). Ntsuas txhua cov hmoov peb zaug ua ke thiab suav H. Cov qauv kev suav suav qhia hauv mis (8). H＝(m7－m6)/(m6－m5) (8)
(9) Particle loj (D10, D50, D90), ncua, SSA, thiab dav: ntsuas los ntawm laser particle loj faib analyzer, nrog huab cua li nruab nrab, ntxiv cov qauv mus rau lub feed hopper ntawm qhuav dispersion system, thiab ntsuas qhov particle loj D10, D50, D90 thiab particle loj SSA sib haum mus rau cumulative particle loj faib tus naj npawb mus txog 10%, 50% thiab 90% feem. Cov qauv kev suav ntawm qhov dav thiab dav yog qhia hauv cov qauv (9) thiab 10).
span 1 (D90 ~ D10) / D50 (9)
Qhov dav (D90) - D10 (10)
2.2.3 Kev ntsuas cov txiaj ntsig ntawm txhua qhov ntsuas nruab nrab ntawm cov hmoov extract
Siv 3 batch ntawm extract hmoov ntawm Scutellaria baicalensis thiab Coptis chinensis qhuav nyob rau hauv 3 txoj kev sib txawv, ntsuas thiab xam tus nqi tiag tiag ntawm txhua qhov ntsuas raws li txoj kev hauv qab "2.2.2", thiab siv tus nqi nruab nrab raws li kev tswj hwm. Cov txiaj ntsig tau pom nyob rau hauv Table 1.
Cov ntaub ntawv qhia tau hais tias qhov tseem ceeb ntawm tag nrho cov qauv yog nyob nruab nrab ntawm 42.20 degree thiab 48.80 degree, tag nrho ntau dua 40 degree, qhia tsis zoo fluidity; qhov nruab nrab qhov tseem ceeb ntawm SR-Da ntawm atmospheric siab kuaj yog 0.49 g/mL, SR-Dc yog 0.95 g/mL, CR-Da yog 0.44 g /mL, thiab CR-Dc yog 0.78 g/mL; qhov nruab nrab qhov tseem ceeb ntawm SR-Da ntawm cov qauv nqus tsev vacuum yog 0.58 g/mL, SR-Dc yog {{30}}.99 g/mL, CR-Da yog {{36} }.49 g/mL, thiab CR-Dc yog 0.78 g/mL; Qhov nruab nrab qhov tseem ceeb ntawm SR-Da ntawm cov tshuaj tsuag yog 0.41 g / mL, SR-Dc yog 0.64 g / mL, CR-Da yog 0.31 g / mL, thiab CR-Dc yog 0.61 g / mL. Nws tau pom tias Da thiab Dc qhov tseem ceeb ntawm atmospheric siab qhuav thiab nqus-qhuav cov qauv ntawm Scutellaria baicalensis extract hmoov txawv. Qhov tseem ceeb yog nyob ze, thiab Da thiab Dc ntawm cov tshuaj tsuag-qhuav cov qauv raug txo qis; HR ntawm tag nrho cov qauv yog 4.39% ~ 5.29%, thiab H yog 9.35% ~ 12.04%. Raws li cov kev cai hygroscopicity ntawm 2020 tsab ntawm Suav Pharmacopoeia [13], thaum 2% ＜H＜15%, nws muaj hygroscopicity. Ua ke nrog kev soj ntsuam ntawm kev txiav txim siab ntawm H, nws tuaj yeem pom tias Scutellaria baicalensis thiab Coptis chinensis muaj qhov pom tseeb hygroscopicity; cov particle loj D50, ncua thiab dav ntawm cov atmospheric siab cov qauv ntawm 36.17 mus rau 44.99 μm, 2.79 mus rau 3.78 thiab 113.70 mus rau 143.15 μm, qhov ntau ntawm D50, ncua thiab dav ntawm lub tshuab nqus tsev kuaj ntawm 3.5 mus rau 45.39μm. 3.26 thiab 145.74 txog 166.13 μm, ntsig txog, thiab particle loj D50, ncua thiab dav ntawm cov tshuaj tsuag cov qauv ntawm 45.39 txog 54.31 μm, 3.05 txog 3.26 thiab 145.74 txog 166.13 μm, ntsig txog Qhov ntau yog 5.02-6.02 μm, 1.46-1.71, thiab 7.26-10.61 μm, feem. Nws tuaj yeem pom tias cov qauv ntsuas ib txwm muaj thiab cov qauv nqus ntawm Scutellaria baicalensis thiab Coptis chinensis muaj qhov sib xws ntawm qhov loj me, qhov loj thiab dav, thiab qhov tsis sib xws ntawm qhov loj me me, thaum D50, qhov dav thiab dav ntawm cov qauv tshuaj tsuag qhuav yog txo qis. , qhia tau hais tias cov hmoov extract tau los ntawm cov tshuaj tsuag kom qhuav muaj qhov me me thiab cov khoom me me.
2.2.4 Tsim kom muaj lub cev ntiv tes spectrum ntawm extract hmoov thiab kev soj ntsuam zoo sib xws
Txij li qhov ntsuas qhov ntsuas ntawm qhov ntsuas lub cev theem nrab muaj qhov sib txawv thiab qhov sib txawv, txhua qhov ntsuas nruab nrab yog qhov qub thiab hloov mus rau tib qhov ntsuas, piv txwv li {{0}}, los ntawm cov qauv. Cov qauv hloov dua siab tshiab ntawm txhua qhov taw qhia theem nrab tau qhia nyob rau hauv Table 2, thiab cov txiaj ntsig kev hloov pauv tau pom nyob rau hauv Table 3. Tom qab ntawd, qhov hloov dua siab tshiab txhais tau tias ntawm txhua qhov ntsuas thib ob uas sawv cev rau thawj qhov ntsuas tau muab ntxiv ua ke, thiab qhov nruab nrab tus nqi raug suav kom tau txais tus nqi. ntawm txhua qhov taw qhia thawj [14-15]. Cov txiaj ntsig tau pom nyob rau hauv Table 4. Cov ntiv tes lub cev ntawm cov hmoov extract yog quantitatively thiab intuitively tso tawm nyob rau hauv daim ntawv ntawm radar daim ntawv. Daim duab radar yog kos rau cov ntaub ntawv hauv Table 3 siv Keeb Kwm software, thiab qhov zoo sib xws yog suav nrog Pearson correlation ntawm SPSS. Piv nrog rau cov kab kos tswj ntawm txhua pab pawg, qhov ze dua qhov zoo sib xws yog 1, qhov zoo sib xws ntawm lub cev lub cev ntawm cov hmoov yog, raws li qhia hauv daim duab 1. Qhov zoo sib xws ntawm lub cev siab qhuav extract hmoov ntawm Scutellaria baicalensis yog {{1 0}}.76, 0.85, thiab 0.94, qhov zoo sib xws ntawm lub tshuab nqus tsev qhuav extract hmoov ntawm Scutellaria baicalensis yog 0.87, {{2 0}}.92, thiab 0.89, thiab qhov zoo sib xws ntawm cov tshuaj tsuag qhuav extract hmoov ntawm Scutellaria baicalensis yog 0.98, 0.99, thiab { {35}}.99. Qhov zoo sib xws ntawm lub tshuab nqus tsev qhuav, tshuaj tsuag-qhuav, thiab lub zog-qhuav Scutellaria baicalensis yog 0.95 thiab 0.69, feem. Nws tau txiav txim siab tias qhov qub siab qhuav zoo sib xws ntawm Scutellaria baicalensis tsis siab, lub tshuab nqus tsev qhuav zoo sib xws yog siab tshaj qhov qub siab, thiab cov tshuaj tsuag-qhuav zoo sib xws yog qhov siab tshaj. Qhov zoo sib xws ntawm atmospheric siab ziab extract hmoov ntawm Coptis chinensis yog 0.89, 0.97, thiab 0.93, qhov zoo sib xws ntawm lub tshuab nqus tsev ziab extract hmoov ntawm Coptis chinensis yog {{ 47}}.98, 0.99, thiab 0.98, thiab qhov zoo sib xws ntawm cov tshuaj tsuag kom qhuav extract hmoov ntawm Coptis chinensis yog 0.99, 1.00 ib 0,99. Qhov zoo sib xws ntawm lub tshuab nqus tsev ziab, tshuaj tsuag kom qhuav, thiab atmospheric siab ziab ntawm Coptis chinensis yog 0.94 thiab 0.61, feem. Cov txiaj ntsig tau pom tias huab cua siab kom qhuav, nqus kom qhuav, thiab tshuaj tsuag kom qhuav ntawm Coptis chinensis yog txhua yam zoo sib xws.
Qhov sib txawv ntawm cov ntiv tes ntiv tes ntawm ntau txoj kev ziab ntawm Scutellaria baicalensis thiab Coptis chinensis tuaj yeem yog vim muaj cov tshuaj sib txawv ntawm Scutellaria baicalensis thiab Coptis chinensis. Thaum lub sij hawm sim, Scutellaria baicalensis thiab Coptis chinensis nyob rau hauv tib txoj kev ziab tau muab tso rau hauv tib qhov chaw, thiab Scutellaria baicalensis sai sai absorbed noo noo thiab clumped; Qhov zoo sib xws ntawm cov ntiv tes ntiv tes spectra ntawm atmospheric siab kom qhuav cov qauv thiab lub tshuab nqus tsev kom qhuav cov qauv ntawm ob yuav yog vim hais tias ob leeg tau ua tib yam crushing thiab sieving txheej txheem, thaum cov tshuaj tsuag-qhuav qauv tsis tau undergone cov txheej txheem no, tab sis nws hais yog finer. thiab ntau uniform.
Cov txiaj ntsig ntawm kev suav ntawm txhua qhov kev ntsuas thawj zaug tau pom nyob rau hauv Table 4. Thaum qhov ntsuas qhov tseem ceeb tshaj 5, qhov kev ua tau zoo tuaj yeem suav tias yog qhov zoo. Cov huab cua siab thiab cov qauv nqus-qhuav ntawm Scutellaria baicalensis muaj cov txheej txheem zoo, ruaj khov zoo, thiab sib xws; cov tshuaj tsuag-qhuav cov qauv muaj zoo uniformity, tab sis kuj tsis zoo stability. Ua ke nrog cov kev ntsuas theem nrab, nws tuaj yeem pom tias nws qhov nqus dej noo (H) kuj tseem siab; xav txog kev ruaj ntseg ntawm lub tshuab nqus tsev qhuav extract hmoov ntawm Scutellaria baicalensis, qhov thib ob indexes HR thiab H ze ze rau kev nqus dej yog qhov zoo tshaj plaws hauv txhua pab pawg. Tsis tas li ntawd, nws cov stacking, fluidity, thiab uniformity yog qhov zoo, uas yog yooj yim rau cov txheej txheem txuas ntxiv ntawm cov khoom npaj xws li tshuaj ntsiav thiab granules. Kev ruaj ntseg thiab sib xws ntawm Coptis chinensis extract hmoov nrog ntau txoj kev ziab kom qhuav yog qhov zoo, thaum lub tshuab nqus tsev thiab cov tshuab nqus tsev qhuav muaj qhov zoo stacking, tab sis tsis zoo compressibility. Lub compressibility theem nrab indexes IC, Ie, thiab SSA ntawm cov tshuaj tsuag-qhuav cov qauv yog loj, thiab nws compressibility yog qhov zoo tshaj plaws. Ua ke D50, IC, Ie, thiab SSA, nws tau txiav txim siab tias cov tshuaj tsuag-qhuav extract hmoov ntawm Coptis chinensis yog qhov ruaj khov thiab zoo ib yam, nrog kev ua kom zoo, uas yooj yim rau kev txhim kho cov txheej txheem tom ntej.
12 lub cev tsis sib txawv ntawm cov khoom sib txawv ntawm cov hmoov extract ntawm Scutellaria baicalensis thiab Coptis chinensis qhuav nyob rau hauv ntau txoj kev tau normalized los ntawm SPSS cov ntaub ntawv thiab cov correlation ntawm cov tsis tau los ntawm Pearson correlation coefficient txoj kev. Rau lub hauv paus no, ib daim ntawv qhia kev sib raug zoo matrix hotspot tau kos, thiab cov txiaj ntsig tau pom hauv daim duab 2. Cov xim tsaus dua thiab cov xim ze dua, qhov zoo sib xws ntawm txhua tus qauv thiab txhua qhov ntsuas lub cev. Hloov pauv, qhov sib zog ntawm cov xim lossis ntau dua qhov sib txawv xim, qhov tsis muaj zog ntawm kev sib raug zoo. Daim duab 2-A qhia txog kev sib raug zoo ntawm txhua tus qauv hauv 12 tus yam ntxwv ntawm lub cev. Nws pom tias cov hmoov extract muaj qhov zoo sib xws hauv Da, Dc, Ie, IH, HR, span, thiab SSA, thiab muaj qhov sib txawv pom tseeb hauv , particle loj D50, thiab dav. Daim duab 2-B yog ob-dimensional matrix correlation ntawm ntau yam tsis muaj lub cev. Cov txiaj ntsig tau pom tias H muaj kev sib raug zoo nrog SSA, ncua, thiab dav (P < 0.05), SSA muaj kev sib raug zoo nrog Dc, Ie, D50, span, thiab dav (P < 0.05), thiab Dc Nws muaj feem cuam tshuam nrog Da, D50, dav, thiab SSA. Yog tias SSA hloov pauv loj, H, Dc, thiab lwm yam tsis muaj feem cuam tshuam kuj tseem yuav hloov pauv raws li, qhia tias hygroscopicity ntawm extract hmoov cuam tshuam los ntawm ntau lub cev.
Daim duab 1 Lub cev ntiv tes ntawm cov hmoov extract ntawm SR thiab CR nyob rau hauv ntau txoj kev kom qhuav
A-kev sib raug zoo ntawm lub cev ntsuas ntawm txawv extract hmoov; B-2D matrix correlation ntawm hmoov indexes.
Fig. 2 Hotspot daim ntawv qhia ntawm qhov zoo sib xws matrix ntawm cov qauv ntsuas
2.2.5 PCA raws li Powder Science
12 lub cev tsis zoo ntawm cov hmoov extract ntawm Scutellaria baicalensis thiab Coptis chinensis nyob rau hauv ntau txoj kev ziab tau raug tshuaj xyuas hauv PCA qauv siv SIMAC software. Cov txiaj ntsig tau pom tias qhov sib txawv ntawm qhov nyiaj pab cuam ntawm tus thawj tswj hwm 1 yog 53.46%, qhov sib txawv ntawm tus thawj tswj hwm 2 yog 26.86%, thiab qhov sib txawv ntawm tus thawj tswj hwm 3 yog 7.24%. Cov lus piav qhia sib txawv ntawm thawj ob lub ntsiab lus tseem ceeb tau nce mus txog 80.32%, uas ua tau raws li qhov yuav tsum tau ua hauv kev txheeb cais [16]. Yog li, qhov kev sim tau xaiv thawj ob lub ntsiab lus tseem ceeb rau kev txheeb xyuas kev txheeb xyuas. Raws li lub load dispersion daim duab tau txais los ntawm PCA, kab rov tav axis sawv cev rau lub hauv paus ntsiab lus ntawm 1 thiab ntsug axis sawv cev rau lub hauv paus ntsiab lus 2. Qhov deb ntawm qhov parameter Performance index yog los ntawm lub hauv paus chiv keeb, qhov ntau dua qhov kev pab cuam rau tus thawj xib fwb tivthaiv. Lub ntsiab Cheebtsam 1 muaj qhov loj tshaj plaws kev pab cuam los ntawm IH, HR, H, Da, Dc, thiab particle loj D50; Lub ntsiab tivthaiv 2 yog tsim los ntawm , IC, Ie, SSA, thiab dav, thiab ncua. Qee qhov kev ntsuas lub cev muaj kev sib koom ua ke. Cov khoom sib txuas qhia tau hais tias kev sib raug zoo ntawm ib leeg muaj zog dua. Ntawm lawv, SSA nyob ze rau qhov dav thiab qhov dav, qhia tias qhov loj me me ntawm qhov dav thiab qhov loj me me muaj qhov cuam tshuam ntau dua rau thaj chaw tshwj xeeb. Cov txiaj ntsig tau pom nyob rau hauv daim duab 3. Daim ntawv qhia PCA qauv kev txheeb xyuas cov qhab nia qhia tau hais tias qhov kev faib tawm ntawm cov hmoov extract ntawm Scutellaria baicalensis thiab Coptis chinensis nyob rau hauv ib txwm siab thiab lub tshuab nqus tsev ziab yog qhov sib txawv, uas qhia txog qhov sib txawv loj hauv lub cev ntawm cov khoom siv; qhov kev faib tawm ntawm cov hmoov nplej ntawm cov tshuaj tsuag kom qhuav yog cov concentrated, thiab qhov hloov pauv me me vim yog lub zog ntawm lub cev. Tsis tas li ntawd, kev faib tawm ntawm cov hmoov extract tau los ntawm kev ziab kom qhuav ntawm Scutellaria baicalensis thiab Coptis chinensis yog tus kheej ywj pheej, uas tseem qhia txog qhov sib txawv tseem ceeb ntawm txoj kev ziab kom qhuav thiab ib txwm ua kom qhuav thiab nqus kom qhuav, uas zoo ib yam nrog cov txiaj ntsig ntawm Kev sib piv ntawm lub cev ntiv tes zoo sib xws, saib daim duab 4.
#cistanche #cistanchedeserticola #cistanchetubulosa #cistanchesalsa #glycoside #echinacoside #verbascoside #acteoside #ニクジュヨウ #肉蓯蓉 #ベルバスコシド #アク #ベルバスコシド #アクキナコシド
Txhawb Kev Pabcuam Ntawm Wecistanche
Email: wallence.suen@wecistanche.com
Whatsapp / Tel: +86 15292862950
Khw Muag Khoom Kom Paub Ntxiv Specifications:
https://www.xjcistanche.com/cistanche-shop
Nyem qhov no kom tau txais Natural organic CISTANCHE EXTRACT nrog 25% ECHINACOSIDE thiab 9% ACTEOSIDE rau lub raum ua haujlwm
