Kev Ntsuam Xyuas Ntawm Kev Ua Haujlwm Los Ntawm Kev Nyuaj Siab Ntawm Tag Nrho Saponins Ntawm Radix Notoginseng

Xu Yong-xin & Zhang Jian-jun

Physical Education College, Zhengzhou University, Zhengzhou, PR Tuam Tshoj

Yog xav paub ntxiv: ali.ma@wecistanche.com

Keeb kwm & lub hom phiaj:

Ntau yam kev ua ub no ntawm tag nrho cov saponins ntawm Radix notoginseng (TSRN), cov tshuaj suav tshuaj tau tshaj tawm. Qhov kev tshawb fawb tam sim no tau ua los tshawb xyuas qhovkev tiv thaiv kev qaug zogntawm TSRN hauv txiv neej Kunming nas.

Txoj kev:

Cov nas tau muab faib ua plaub pawg. Thawj pab pawg tau xaiv los ua pawg tswj hwm tau muab cov dej distilled los ntawm gavage txhua hnub. Cov pab pawg thib ob, thib peb, thiab thib plaub uas tau xaiv los ua TSRN cov pab pawg kho mob tau tswj hwm nrog TSRN ntawm 20, 40, thiab 80 mg / kg lub cev hnyav / hnub, raws li. Kev kho mob txuas ntxiv rau 28 hnub. Lub sijhawm ua luam dej, ntshav lactate, thiab cov ntaub so ntswg glycogen ntawm cov nas tom qab ua luam dej tau txiav txim siab.

Cov txiaj ntsig:

TSRN ncua sij hawm ua luam dej tag nrho ntawm cov nas, ua haujlwm ncua ntev ntawm lactate hauv cov ntshav, thiab nce cov ntaub so ntswg glycogen.

Kev txhais & lus xaus:

TSRN tau pom zookev tiv thaiv kev qaug zognyob rau hauv tus qauv tsiaj. Txawm li cas los xij, kev kawm ntxiv yog xav tau los piav qhia txog cov txheej txheem ntawm kev cuam tshuam ntawm TSRN rauqaug zog.

Cov lus tseem ceeb: Kev ua kom qaug zog - lactate - ua luam dej - tag nrho saponins ntawm Radix notoginseng

Panax notoginseng (Burk.) FH Chen tau cog qoob loo thoob plaws Southwest Tuam Tshoj, Burma, thiab Nepal. Lub hauv paus, feem ntau siv los ntawm cov nroj tsuag no hu ua Radix notoginseng los yog Sanchi, muaj keeb kwm ntev los ua kev kho mob hauv cov tshuaj oriental tshuaj 1-3. Nyob rau hauv Suav teb, R. notoginseng yog siv los txhawb cov ntshav ncig, tshem tawm cov ntshav stasis, ntxias cov ntshav txhaws, txo qhov o, ncua sij hawm.qaug zog,thiab txo qhov mob 4,5. R. notoginseng tau tshaj tawm tias muaj txiaj ntsig zoo rau cov kab mob hauv lub plawv, kab mob hauv lub hlwb, mob qog noj ntshav, ntshav qab zib mellitus nrog rau kev kawm thiab kev nco txhim kho hauv cov kev tshawb fawb sim 6-8. Cov teebmeem kev kho mob no yog vim nws cov khoom xyaw nquag, uas yog saponins, flavonoids, thiab polysaccharides9-11. Tag nrho cov saponins ntawm R. notoginseng (TSRN) raug suav hais tias yog cov khoom xyaw tseem ceeb thiab yog qhov sib xyaw ntawm ntau tshaj 20 Dammarane hom saponins, suav nrog ginsenoside Rg1, Rg2, Rb1, Rb2, Rb3, Rc, Rd, Re, Rh, F2. , thiab notoginsenoside R1, R2, R3, R4, R6, Fab, Fc, Fe, thiab lwm yam 12. Txawm hais tias muaj ntau yam kev ua ub no lom neeg thiab cov tshuaj ua haujlwm ntawm TSRN paub. Nws paub tias muaj qee qhov kev tshawb fawb tshawb xyuas nws cov teebmeem ntawmlub cev qaug zog. Yog li ntawd, qhov kev tshawb fawb tam sim no tau tsim los tshawb xyuas qhovantifatigue kev uantawm tag nrho cov saponins ntawm R. notoginseng nyob rau hauv ib tug nas qauv.

Khoom siv & txoj kev

Txoj kev tshawb no tau ua nyob rau hauv Laboratory ntawm Biochemistry, Zhengzhou University (Zhengzhou, Suav). Cov ntaub ntawv cog: Dried R. notoginseng qauv tau txais los ntawm Henan Suav Herbal Medicine Company, (Zhengzhou, Tuam Tshoj), thiab tau lees paub los ntawm Department of Botany, Zhengzhou University (Zhengzhou, Tuam Tshoj.). Cov qauv no tau txheeb xyuas los ntawm macro-morphological thiab microscopic yam ntxwv thiab nyias-txheej chromatography (TLC). Raws li Suav Pharmacopoeia, nws tau txheeb xyuas tias yog lub hauv paus ntawm

Panax notoginseng (Burk.) FH Chen. Kev npaj tag nrho saponins ntawm R. notoginseng (TSRN):

Qhuav R. notoginseng yog av rau hmoov thiab dhau los ntawm 40 mesh sieve. TSRN tau npaj los ntawm txoj kev Sun thiab al13. Hauv luv luv, cov qauv siv hluav taws xob (1 kg) tau muab rho tawm nrog 70 feem pua ​​​​ethanol ntawm 100 degree (3 × 4 l), thiab concentrated hauv lub tshuab nqus tsev (40 degree) kom evaporate cov kuab tshuaj kom muab lub ntim me me. Tom qab rho tawm nrog ether (3 × 0.5 l), cov txheej txheej dej tau muab rho tawm nrog n-Butanol kom txog thaum n-Butanol txheej tsis muaj xim. Cov tshuaj n-Butanol tau concentrated thiab qhuav hauv lub tshuab nqus tsev (60 degree). Cov extract qhuav tau raug rau D101 resin kem chromatography, ntxuav nrog H2O, thiab eluted nrog Etanol muab TSRN. TSRN muaj 64.3 ± 1.15 feem pua ​​​​ntawm notoginsenoside raws li kev txiav txim los ntawm nyias txheej chromatography thiab spectrophotometric txoj kev.

Tsiaj txhu:

Txiv neej Kunming nas, hnyav nruab nrab ntawm 18- 22 g, tau txais los ntawm Laboratory Tsiaj Center, Medical College ntawm Zhengzhou University (Zhengzhou, Suav teb.), thiab tau noj zaub mov ua lag luam thiab dej ad libitum. Kev lag luam noj zaub mov muaj 12% rog, 60% carbohydrate, thiab 28% protein. Cov tsiaj tau nyob hauv qab 12-h lub teeb / tsaus voj voog ntawm qhov kub ntawm 22 ± 1 degree thiab av noo ntawm 50 ± 5 feem pua. Cov nas tau tso cai rau acclimate rau qhov chaw kuaj mob tsawg kawg 1 lub lis piam ua ntej qhov kev sim. Kev coj ncaj ncees clearance rau kev ua cov kev sim ntawm cov tsiaj tau txais los ntawm Institutional Animal Ethics Committee.

Kev sib faib ntawm cov tsiaj:

Cuaj caum-rau nas tau muab faib ua plaub pawg, txhua tus muaj 24 nas. Thawj pab pawg tau xaiv los ua pawg tswj hwm (Control) tau muab cov dej distilled los ntawm gavage txhua hnub. Cov pab pawg thib ob, thib peb, thiab thib plaub uas tau xaiv los ua TSRN pab pawg kho mob tau tswj hwm nrog TSRN ntawm 20, 40, thiab 80 mg / kg lub cev / hnyav hnub, raws li. Kev tswj hwm cov dej distilled lossis TSRN tau txuas ntxiv rau 28 hnub. Cov koob tshuaj ntawm TSRN thiab 28 hnub ntawm lub sijhawm kho mob siv hauv txoj kev tshawb no tau lees paub tias tsim nyog thiab siv tau zoo hauv cov nas kuaj, raws li kev sim ua ntej.

Exhaustive ua luam dej xeem:

Tom qab kev kho zaum kawg nrog TSRN lossis dej distilled, cov nas raug tso cai so rau 30 feeb. Tom qab ntawd, yim nas raug tshem tawm ntawm txhua pab pawg rau kev sim ua luam dej tag. Cov tsiaj tau muab tso rau hauv lub tank ua luam dej (50 × 50 × 40 cm) 30 cm tob nrog dej tuav ntawm 25 ± 2 degree. Tus Tsov tus tw ntawm txhua tus nas tau ntim nrog ib pob ntawm cov hlau lead, uas yog 10 feem pua ​​​​ntawm nws lub cev hnyav. Kev qaug zog tau txiav txim siab los ntawm kev soj ntsuam qhov poob ntawm kev sib koom tes thiab tsis ua tiav rov qab mus rau qhov chaw hauv 10 sec14. Lub sijhawm ua luam dej tau sau tseg tam sim ntawd.

Ntsuas biochemical tsis muaj feem xyuam rau qaug zog:

Tom qab kev kho zaum kawg nrog TSRN lossis dej distilled, cov nas raug tso cai so rau 30 feeb. Tom qab ntawd, yim nas raug tshem tawm ntawm txhua pab pawg rau kev ntsuas ntshav lactate. Cov nas raug yuam ua luam dej rau 30 feeb tom qab qhov hnyav (2 feem pua ​​​​ntawm lub cev hnyav), thiab cov ntshav tau sau los ntawm tus Tsov tus tw leeg ua ntej thiab tom qab ua luam dej. Cov ntshav lactate cov ntsiab lus raug ntsuas raws li cov txheej txheem pom zoo muab los ntawm cov khoom siv tshuaj ntsuam xyuas kev lag luam (Nanjing Jiancheng Bioengineering Institute, Jiangsu, Suav). Lwm yim nas raug tshem tawm ntawm txhua pab pawg rau kev tshuaj xyuas cov ntaub so ntswg glycogen. Cov nas raug yuam ua luam dej rau 90 feeb yam tsis muaj kev thauj khoom. Tom qab so rau ib teev, nas raug tua los ntawm lub ncauj tsev menyuam dislocation nyob rau hauv tshuaj loog. Lub siab thiab gastrocnemius cov leeg tau sau 15. Cov ntaub so ntswg glycogen tau sim raws li cov txheej txheem pom zoo muab los ntawm cov khoom siv tshuaj ntsuam xyuas kev lag luam (Nanjing Jiancheng Bioengineering Institute, Jiangsu, Suav).

Kev txheeb cais

: Tag nrho cov kev ntsuam xyuas tau ua nyob rau hauv triplicate. Cov ntaub ntawv sim tau qhia tias txhais tau tias ± tus qauv sib txawv. Ib txoj kev tsom xam ntawm qhov sib txawv (ANOVA), LSD thiab Dunnett's T3 kev xeem tau ua los txiav txim siab qhov sib txawv ntawm cov qauv hauv 95 feem pua ​​​​ntawm kev ntseeg siab, siv SPSS 13.0 software (SPSS Inc., Chicago, IL, USA).

Cov txiaj ntsig

Cov teebmeem ntawm TSRN rau lub sijhawm ua luam dej ntawm nas:

Lub sijhawm ua luam dej ntawm cov pab pawg thib peb thiab plaub tau nce ntau (P<0.05) when="" compared="" with="" the="" control="" group.="" however,="" the="" exhaustive="" swimming="" time="" of="" the="" second="" group="" showed="" no="" significant="" changes="" compared="" with="" the="" control="" group="" (fig.="" 1).="" the="" swimming="" time="" of="" the="" second,="" third,="" and="" fourth="" groups="" increased="" by="" 21.15,="" 27.41,="" and="" 34.01="" percent,="" respectively.="">

Cov teebmeem ntawm TSRN ntawm cov ntshav lactate cov ntsiab lus ntawm cov nas tom qab ua luam dej:

Tsis muaj qhov sib txawv tseem ceeb hauv cov ntshav lactate cov ntsiab lus ntawm TSRN cov pab pawg kho mob thiab pawg tswj hwm ua ntej ua luam dej. Tom qab ua luam dej, cov ntshav lactate cov ntsiab lus ntawm txhua pab pawg kho mob TSRN poob qis (P<0.05) when="" compared="" to="" the="" control="" group="" (fig.="" 2).="" the="" results="" indicated="" that="" the="" blood="" lactate="" contents="" of="" the="" second,="" third="" and="" fourth="" groups="" decreased="" by="" 47.14,="" 57.59,="" and="" 61.96="" percent,="">

Cov teebmeem ntawm TSRN ntawm cov ntaub so ntswg glycogen ntawm cov nas tom qab ua luam dej:

Tom qab ua luam dej, daim siab, thiab cov leeg nqaij glycogen ntawm txhua pawg TSRN kho tau nce ntau (P<0.05) when="" compared="" with="" that="" of="" the="" control="" group="" (fig.="" 3).="" the="" liver="" glycogen="" contents="" of="" tsrn="" treatment="" groups="" increased="" by="" 58.12,="" 115.84,="" and="" 153.58="" percent,="" respectively.="" the="" muscle="" glycogen="" contents="" of="" treatment="" groups="" increased="" by="" 58.54,="" 83.74,="" and="" 73.98="" percent,="">

Kev sib tham

Txoj kev tshawb no tau tsim los tshawb xyuas cov haujlwm tiv thaiv kev qaug zog ntawm tag nrho saponins ntawm R. notoginseng (TSRN). Kev quab yuam ua luam dej ntawm cov tsiaj tau ua haujlwm raws li cov qauv ntawm lawv lub cev ua haujlwm. Ntau qhov kev tshawb fawb tau taw qhia tias kev ua luam dej muaj txiaj ntsig zoo dua lwm hom kev tawm dag zog, suav nrog treadmill 16-19. Txhawm rau ua tus qauv ua haujlwm thiab txo lub sijhawm ua luam dej, qhov hnyav ntawm qhov feem pua ​​​​ntawm lub cev hnyav tau ntxiv rau hauv siab lossis tus Tsov tus tw ntawm tus tsiaj 17,20. Txoj kev tshawb fawb tam sim no tau qhia tias TSRN tau txuas ntxiv lub sijhawm ua luam dej ntawm cov nas, uas qhia tias TSRN muaj kev tiv thaiv kev qaug zog thiab tuaj yeem ua rau muaj kev ua haujlwm siab ntev. Txhawm rau tshawb xyuas cov txheej txheem, qee qhov kev ntsuas biochemical tau txiav txim siab hauv cov nas tom qab ua luam dej. Ntshav lactate yog cov khoom glycolysis ntawm carbohydrates nyob rau hauv ib qho kev mob anaerobic, thiab glycolysis yog lub zog tseem ceeb rau kev tawm dag zog hnyav hauv lub sijhawm luv luv21,22. Yog li, ntshav lactate yog ib qho ntawm cov cim tseem ceeb rau kev txiav txim siab txog qib ntawm kev ua kis las. Hauv kev tshawb fawb tam sim no, TSRN tau ua haujlwm tau zoo ncua kev nce lactate hauv cov ntshav thiab cov tsos ntawm qaug zog. Lub zog rau kev tawm dag zog yog pib los ntawm kev tawg ntawm glycogen, tom qab kev tawm dag zog ntawm cov leeg glycogen yuav tawm mus, thiab tom qab ntawd, lub zog yuav los ntawm cov piam thaj tso tawm los ntawm lub siab. Yog li, lub siab thiab cov leeg nqaij glycogen cov ntsiab lus yog qhov tsis zoo rau kev qaug zog 23-25. Hauv kev tshawb fawb tam sim no, TSRN tau nce cov ntaub so ntswg glycogen ntawm cov nas tom qab ua luam dej. Hauv kev xaus, cov txiaj ntsig tau qhia tias TSRN muaj kev tiv thaiv kev qaug zog, uas txuas ntxiv lub sijhawm ua luam dej ntawm cov nas, ua kom muaj txiaj ntsig zoo ncua kev nce lactate hauv cov ntshav, nrog rau nce cov ntaub so ntswg glycogen. Kev kawm ntxiv yog xav tau los piav qhia qhov tseeb ntawm kev cuam tshuam ntawm TSRN ntawm kev qaug zog.

Nov yog peb cov khoom tiv thaiv kev qaug zog! Nyem rau daim duab rau cistanche tubulosa ntau npaum

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