Kev Kawm Network Kev Kawm nyob rau estrogenic-zoo li cov nyhuv ntawm glycosides ntawm cistanche ntawm cistanche Squarance

 

Tsis zoo siv

Txoj kev kawm no tshawb txog cov peev txheej ntawm estrogenic zoo li cov teebmeem ntawmglycosides ntawmCistanche Squaticola(GCS)Raws li cov chaw muag tshuaj network ua ke nrog cov ntaub ntawv transcriptic. Tag nrho ntawm40 nquag cov khoom xyawntawm GCS nrog cov hluav taws xob hluav taws xob tau kwv yees siv cov ntaub ntawv uas siv cov ntaub ntawv xws liTcmsp, Symmap, Lwm yam, Saum, thiabTheem.

Cov ntaub ntawv hom phiaj ntawm cov kab mob estrogenic tsis txausTus davthiabLeej txivdatabases. Cov hom phiaj tseem ceeb tau tshuaj ntsuam, thiab aPPI Networktau tsim.Mus ua haujlwm kev txheeb xyuas kev ua haujlwmthiabKEGGH Kev Ntsuam Xyuas Kev Tshawb Fawbtau ua los txheeb xyuas cov hom phiaj tseem ceeb thiab txoj hauv kev.

Ua ke nrog cov ntaub ntawv txuas ntxiv ua ke, kev txheeb xyuas qhov sib txuam ntawm cov pob tshuaj kwv yees cov phiaj xwm thiab Keg Grampaphory tau ua tiav los qhia txog kev ua kom pom kev ua haujlwm estrogenic-zoo li cov nyhuv ntawm GCS.

183 lub hom phiajtau kuaj xyuas, thiab1,712 phiajtau raug xaiv rau cov kab mob estrogen tsis txaus.

24 Lub Hom Phiaj Tseem Ceebtau txais.

Mus ua haujlwm thiab Keg Gughment txhawb nqa tau tias GCS feem ntau ua raws li cov txheej txheem biological xws li:

CyTOKine Receptor Binding

Estrogen receptor binding

Kev Loj Hlob Kev Loj Hlob

Nuclear Receptor Ua Haujlwm

Nuclear Hormone Receptor Binding

SteroOoG hormone receptor kev ua haujlwm

Hormone receptor binding

Cov kev tswj hwm txoj cai muaj xws li:

Qum Necrosis Qhov Tshwj Xeeb (TNF) Kev Tawm Tsam Txoj Kev

Interlekin -17 (is -17}) Kev Pom Txoj Kev

Hypoxia-inducible factor -1 (hif -1) Kev piav qhia txoj hauv kev

Estrogenen Signaling Txoj Kev

Cov ntsiab lus ntawm cov neeg tau txais kev hloov pauv tau pom hauv txhua qhov24 ntxhais lub hom phiajtsuasMCL1thiabTus paCov. Cov ntsiab lus txawv tau suav nrogAR, CASP8, ETRR1, Fos, thiabHMOX1Cov. Tag nrho ntawm68 Khaw Pathwaystau txheeb pom, nrog tusEstrogenen Signaling Txoj Kev, kev pw hav zoov txoj hauv kev, thiabTNF Signaling Txoj Kevua qhov tseem ceeb txoj kev.

Tag

Glycosides ntawmCistanche SquaticolaYuav tau txais cov hluavtogen zoo li extrogen zoo li cov teebmeem los ntawm "ntau feem, ntau-hom phiaj, ntau-pathway" mechanism. Cov txheej txheem no yuav cuam tshuam nrog txoj cai ntawm:

Estrogenen Signaling Txoj Kev

kev pw hav zoov txoj hauv kev

TNF Signaling Txoj Kev

 

 

 

Ntuj Cistanche Extract Tshuaj Tsis TxhobKev tawmticetic

WhatsApp Peb rau cov lus qhia ntxiv

 

 

Lo lus tseem ceeb

Glycosides ntawmCistanche Squaticola

Kev tawmticetic

Network Pej Xeem Pej Xeem

Transcriptomics

Kev Ntsuam Xyuas Kev Sib Koom Tes

 

Tshuaj estrogenyog cov hormone tseem ceeb hauv kev txhim kho cov poj niam kev sib deev thiab cov cwj pwm tsis zoo nyob rau hauv kev tswj hwm cov poj niam fertility thiab poj niam txiv neej [1]. Tom qab nkag mus rau lub cev lawm, cov poj niam ua rau txo cov khoom siv tshuaj ntsuab, uas nyob rau hauv cov kab mob Esterositosis, atherosclerosis, menopaial syndrome, thiab mob plawv [2]. Cov tshuaj estrogen hloov kho tau kho tau los yog txo cov tsos mob tshwm sim los ntawm cov tshuaj estrogen tsis txaus, tab sis qhov kev kho mob no kuj yuav ua rau muaj kev phiv tshuaj [3]. Cov kev tshawb fawb tau pom tias kev siv tshuaj estrogen ntev yuav ua rau muaj kev nkag siab ntev, uamine fibroids, thiab cov memary caj dab, ntawm lwm cov kev tsis zoo. Li no, nrhiav kev siv tau zoo, muaj kev nyab xeeb, thiab siv tau los txhim kho cov tshuaj estrogen tsawg yog qhov tseem ceeb thiab muaj nuj nqis [4].

Nroj tsuag Estrogens yog cov tshuaj muaj peev xwm siv tau estrogen-zoo li kev ua ub no. Lawv cov qauv molecular zoo ib yam li ntawm cov tsiaj Mammalian estrogen, ua rau lawv muaj kev khi tshwj xeeb rau estrogen receptors thiab ua rau cov hluav taws xob estrogen zoo li [5]. Kev tshawb fawb qhia tias cov nroj tsuag ntsuab muaj cov teebmeem ntau yam tshuaj, suav nrog kev tiv thaiv kab mob epteeoporosis thiab hloov cov metabolic tsis muaj xwm txheej [6].

Cistanche Squaticolayog tus qhuav, fleshy qia nrog barky yoojyim ntawm orobanchaceae nroj tshuagCistanche Showicola YC MACCov. Nws muaj cov haujlwm xws li Tasinging Raumney Yang, enricking essence thiab ntshav, thiab noo noo cov hnyuv los daws cov quav tawv. Nws yog ib qho siv tau los kho raum tsis txaus, essence thiab ntshav tsis muaj zog, mob siab, thiab cem quav, thiab quav tawv tshwm sim los ntawm kev mob plab zom mov [7]. Cov kev tshawb fawb dhau los los ntawm pab pawg tshawb fawb paub tseeb tias tag nrho glycosides ntawmCistanche Squaticola(GCS), nrog cov ntsiab lus ntawm ntau dua 60% ntawm Echinacoside, yog lub ntsiab tseem ceeb ntawm lub luag haujlwm rau nws cov kev cuam tshuam estrogen. GCS tseem ceeb nce upine coefficient thiab qhov siab ntawm endometrial pells hauv cov nas tsw. Cov ntsiab lus tsom kwm tshwm sim tau pom tias GCS exert estrogen-zoo li cov teebmeem los ntawm 2,953 cov ntsiab lus txawv, ntawm uas 1,475 tau raug tsa. Cov noob no yog feem ntau cuam tshuam nrog cov lus nug estrogen, zoo kev cai ntawm nitrogen compound cov txheej txheem metabolic, cov txheej txheem kev txhim kho, thiab cov txheej txheem txheej txheem biological. Lawv feem ntau koom nrog hauvEstrogenen Signaling Txoj Kev, kev pw hav zoov txoj hauv kev, RAP1 Kev Sau Npe Txoj Kev Pathway, thiabMapk signaling txoj kev [8-9].

Tsoos suav tshuaj (TCM) Network Party Care Tshawb nrhiav TCM hauv cov hom phiaj ntawm cov hom phiaj ({10-12]. Txhawm rau qhia cov txheej txheem ntawm cov qauv tshuaj estrogen zoo li ntawm GCS, qhov kev kawm no ua haujlwm rau cov pab pawg yav dhau los cov ntaub ntawv los ntawm kev ua tau zoo tag nrhoglycosides ntawmCistanche Squaticola exert Esterrogen-zoo li cov teebmeem.

1. Kev sim databases thiab software

Tcmsp: http://lsp.nkwu.edu.cn/cmsp.php

Symmap: https://www.symap.org

Lwm yam: http://www.nrc.ac.cn :9090/etcm/

Saum: http://stitch.emb.de/

Theem: http://targetNet.scbdd.com/

Tus noog: https://pubchem.ncbi.nih.gov

Tsis muaj uniprot: http://www.uniprot.org/

Tus dav: http://www.genecards.org/

Leej txiv: https://www.omim.org/

Txoj xov: https://string-db.org/

Kegg: https://www.kegg.jp/

Mus: http://geneontology.org/

R lus: RX {64 3. 5.3 Nrog cov pob hauv qab no:

Venndiagram

Suav Teb.r

nruab nrab

org.hs.eg.db

cim dab tsi2id

Perl Tsab Ntawv: Adycbol.pl

Cytoscape: Version 3.7.1

 

2. Cov kev sim ua

2.1 Network Network Porticy Pathriction ntawm GCS 'estrogen-zoo li mechanism

2.1.1 twv ua ntej ntawm cov hom phiaj ntawm GCS 'lub ntsiab tseem ceeb tivthaiv

Siv UPLC / Q-Tof-MS kev tsom xam, 40 sib txuas nrog cov khoom lag luam estrogen-zoo li tshuaj ntsuam xyuas tau txheeb xyuas [13]. Cov tebchaw tau tshawb hauvTcmsp, Symmap, thiabLwm yamDatabases nrhiav cov hom phiaj noob muaj peev xwm rau txhua qhov sib txuas. Tsis tas li ntawd, sib xyaw smiles cov qauv tau raug ntshuam rau hauvSaumthiabTheemdatabases los sau cov hom phiaj. Txhua lub hom phiaj khaws tau sib xyaw, thiab cov ntawv luam tau raug tshem tawm kom tau txais cov hom phiaj muaj peev xwm.

2.1.2 estrogen-zoo li cov ntaub ntawv hauv lub hom phiaj database

Cov ntsiab lus "estrogen deficiency" tau siv los tshawb rau cov hom phiaj tseem ceeb hauvTus davthiabLeej txivdatabases.

2.1.3 Kev tsim kho ntawm "action compound - Cov hom phiaj tseem ceeb - estrogen-zoo li" network

Siv covVenndiagramPob hauv R, cov kev sib tshuam ntawm cov phiaj los ntawm ob qhov chaw tau npaj ua daim duab Venn. Tus neeg sib tw protein sib cuam tshuam cov ntaub ntawv raug ntshuam musCytoscape v3.9.1, thiab cov hom phiaj tseem ceeb tau txheeb xyuas. Cov ntsiab lus sib txuas ntawm cov hom phiaj tseem ceeb tau raug xaiv, thiab cov "sib txuas - cov phiaj xwm - estrogen-zoo li" network tau pom zoo sivCytoscape v3.9.1.

2.1.4 Kev tsim kho ntawm PPI network

Cov ntaub ntawv phiaj xwm tseem ceeb tau raug ntshuam rau hauvTxoj xov database, selecting the species "Homo sapiens" and setting the confidence score to >{{0 {0}}. 7 Los tsim cov protein-protein sib cuam tshuam (PPI). TusSuav Teb.rPob hauv R tau siv los tshawb xyuas thiab xaiv cov ntsiab lus saum toj kawg nkaus nrog kev sib txuas siab tshaj plaws ntawm cov hom phiaj tseem ceeb, uas tau pom.

2.1.5 Mus ua haujlwm ntsuas kev ua haujlwm thiab KeGGAs tsom xam txoj kev ntsuas

Rau cov hom phiaj tseem ceeb, mus thiab Keyg cov kev tshawb nrhiav tau ua siv siv cov pob khoomorg.hs.eg.db, cim dab tsi2id, thiabnruab nrabCov. Sab saum toj 20 txoj kev nrog kho P-Nqi tau pom raws li cov kab kos bar, kev txheeb xyuas cov ntsiab lus tseem ceeb ntawm GCS 'estrogen-zoo li cov teebmeem.

2.2 Kev sib xyaw nrog cov ntaub ntawv transcriptic

2.2.1 kev txheeb xyuas cov ntsiab lus ntawm kev kwv yees thiab cov phiaj xwm transcriptic

Txij li cov tsiaj hauv network porticy kev twv kwv yees yog tib neeg, cov hom phiaj tau hloov pauv mus rau hauv homologous nas sivHgncNtawm lub "cuab yeej-HCOP" muaj nuj nqi. Cov qib kev hais tawm ntawm cov noob sib txawv tau raug tshuaj xyuas nruab nrab ntawm GCS pab pawg thiab pawg tswj hwm hauv cov txheej txheem kev ua ntu zus [9].

 

2.2.2 Kev Ntsuam Xyuas Txoj Kev Nruab Nrab Nruab Nrab Nruab Nrab ntawm Network Pharpacology kev twv thiab cov transcriptimic kegg txoj kev

Cov hom phiaj tseem ceeb tau kwv yees los ntawm Network Pharmacology (hloov pauv hom duab hom) tau tshawb hauv cov keggsabase siv cov pob khoombioconductorthiabnruab nrabnrog rauid2symbol.pltsab ntawv. Cov tsiaj tau txwv rau "RNO," thiab txoj kev nrog Padject <0. 05 tau tshuaj xyuas. Venn txheeb xyuas tau ua los txheeb xyuas cov kev sib tw sib txuam ntawm cov pathwing los ntawm network portiction kev twv xyuas thiab cov kev sim ntawm GCS Estrogen zoo li cuam tshuam.