Siab Soluble CD163 Predicts Renal Function Deterioration in Lupus Nephritis: A Cohort Study in Eastern China

Yog xav paub ntxiv. tiv taujtina.xiang@wecistanche.com

Abstract

Lub Hom Phiaj: Txoj kev tshawb fawb no tau tshawb xyuas qhov kev sib koom ua ke ntawm kev sib cais ntawm cov khoom siv scavenger receptor sib txawv antigen 163 (sCD163) thiab qhov hnyav thiab qhov tshwm sim ntawmmob raumnyob rau hauv lupus nephritis (LN).

Txoj Kev: Ntshav sCDI63 qib hauv 12I Eastern Suav cov neeg mob nrog LN uas tau ua rau lub raum biopsy tau txiav txim siab los ntawm enzyme-linked immunosorbent assays. Cov ntaub ntawv kho mob tau sau tseg, thiab ntsuas glomerular filtration rate thiab kab mob kev ua haujlwm ntawm lupus raug xam. Kev faib tawm ntawm cov kab mob pathological tau ua tiav, thiab cov qhab nia ntawm lub raum pathological raug ntsuas los ntawm qhov ntsuas kev ua haujlwm (Al) thiab qhov ntsuas ntev ntev (Cl). Kaplan-Meier txoj kev ciaj sia taus tau raug kos los ntsuas kev ntsuas. Cov txiaj ntsig: Cov kev faib tawm pathological, Al, thiab CI cov qhab nia hauv pawg sCD163 siab tau nce. Cov qib sCD163 tau sib raug zoo nrog cov ntshav creatinine, ntshav urea nitrogen, Al cov qhab nia, thiab CI cov qhab nia thiab tsis zoo cuam tshuam nrog qhov kwv yees glomerular pom tus nqi. Kaplan-Meier ciaj sia taus tsom xam pom tias qhov tshwm sim ntawm lub raum endpoint txheej xwm tau nce nyob rau hauv siab sCDI63 pab pawg neeg piv nrog rau ib txwm sCD163 pawg. Cov ntsiab lus: Qib sCD163 cuam tshuam nrog qhov hnyav ntawm LN thiab yog ib qho tseem ceeb qhia txog kev mob raum tsis zoo rau cov neeg mob LN.

Ntsiab lus:Soluble CDI63, lupus nephritis, raum raug mob, macrophage, autoimmune, serum marker

Nyem kom paub txog cov txiaj ntsig cistanche Reddit thiab yuav ua li cas cuam tshuam rau lub raum ua haujlwm

Taw qhia

Systemic lupus erythematosus (SLE) yog ib qhoautoimmunekab mob sib txuas, feem ntau cuam tshuam nrog daim tawv nqaij, pob qij txha, ntshav, cov hlab ntsha, thiablub raum. Lupus nephritis (LN) yog qhov tshwm sim tshaj plaws hauv 50 feem pua ​​​​rau 70 feem pua ​​​​ntawm cov neeg mob SLE, thiab 25 feem pua ​​​​ntawm cov neeg mob LN nws thiaj li tsim cov kab mob raum kawg (ESRD).'Immune cells thiab lawv cov secreted soluble effector molecules koom nrog. nyob rau hauv txoj kev loj hlob ntawm LN, thiab yoog raws kev tiv thaiv kab mob los ntawm Blymphocytesthiab T lymphocytes plays lub luag haujlwm tseem ceeb.2 Raws li ib qho tseem ceeb ntawm kev tiv thaiv kab mob hauv lub cev thiab yoog raws, macro-phages muaj ntau lub zog tiv thaiv kab mob, xws li kev nthuav tawm antigen, phagocytosis, thiab cytokine ntau lawm. Dysregulated qhia ntawm macrophage nto markers, cytokine ntau lawm, phagocytosis, thiab clearance ntawm cellular khib nyiab txhawb kev txawv txav thiab proliferation ntawm lymphocytes, ua rau txoj kev loj hlob ntawm autoantigenic thiabmobteb. Yog li, lub luag haujlwm tseem ceeb ntawm macrophages hauv LN tau ua rau muaj kev xav ntau ntxiv.3 Scavenger receptor sib txawv anti-gen 163 (CD163) yog ib leeg-chain transmembrane glycoprotein uas yog thawj pawg ntawm scavenger receptor superfamily B, nrog rau qhov hnyav ntawm kwv yees li. 130 kDa.sCD163 muaj nyob rau hauv cov ntshav thiab lwm cov ntaub so ntswg thiab yog ib qho cim tshwj xeeb ntawm kev ua kom macrophage. Kev qhia ntawm sCD163 thiab heme oxidase yog upregulated nyob rau hauv cov kab mob inflammatory teb, uas tej zaum yuav muaj feem xyuam rau qhov pib ntawm cov dej tsaws tsag secretion ntawm anti-inflammatory yam.4 Serum sCD163 concentrations nyob rau hauv peripheral cov ntshav qhia txog cov kab mob hnyav nyob rau hauv cov kab mob autoimmune, xws li mob caj dab rheumatoid, kab mob sclerosis, thiab polymyositis/dermatomyositis.5-7 Tsis tas li ntawd, tus naj npawb thiab zaus ntawm CD163-cov hlwb zoo (M2 macrophages) yuav ua rau mob hnyav tom qab streptococcal glomerulonephritis, qhia tias qhov mob ntawm endothelial raug mob. tej zaum yuav muaj feem xyuam rau qhov sib txawv ntawm cov phenotypes ntawm infiltrating inflammatory cells. Kev soj ntsuam ntawm kev sib raug zoo ntawm sCD163 thiab kev ua haujlwm ntawm tus kab mob SLE tau tsim cov txiaj ntsig tsis sib xws, - thiab muaj qee yam kev tshawb fawb. Txoj kev tshawb no tau txheeb xyuas cov yam ntxwv kho mob, kuaj tsis tau, thiab lub raum pathology ntawm cov neeg mob LN thiab tshawb nrhiav qhov cuam tshuam ntawm sCD163 thiab lub raum raug mob los txheeb xyuas cov cim tshiab rau kev kuaj mob thiab kev ntsuas ntawm LN, yog li muab lub hauv paus theoretical rau cov hom phiaj kho tshiab hauv LN. .

Cov ntaub ntawv thiab cov txheej txheem

Kawm cov pej xeem

Patients with LN who underwent renal biopsy from 1 January 2014 to 1 June 2018 at tertiary hospitals (Affiliated Hospital of Nantong University, Affiliated Hospital of Jiangnan University, and Nanjing Medical University Affiliated Wuxi Second Hospital)in Eastern China were selected as the study subjects, and healthy individuals were selected as the control group. All patients with LN were scored on the Disease Activity Index using the 2000 revision of the SLE Disease Activity Index(SLEDAI). Participants were followed-up until the occurrence of a renal endpoint event or the study termination date(1 January 2019). Renal endpoints in this study were defined as the occurrence of ESRD, estimated glomerular filtration rate (eGFR)reduction >50 feem pua ​​​​lossis ntau dua lossis sib npaug rau 2-fold nce hauv cov ntshav creatinine (SCr). Cov txheej txheem suav nrog yog raws li hauv qab no: (1) cov neeg mob tau ntsib SLE thiab LN cov txheej txheem kuaj mob2 thiab (2) kuaj LN tseeb los ntawm lub raum biopsy. Cov txheej txheem cais tawm hauv qab no tau siv: (1) cov neeg mob uas muaj lwm yam kab mob tiv thaiv kab mob, xws li scleroderma lossis polymyositis / dermatomyositis, (2) cov neeg mob uas muaj kab mob ntev thiab mob hnyav hauv 3 lub hlis dhau los, (3) cov neeg mob uas muaj kab mob hnyav ntawm cov pa ua pa, circulatory, hematological thiab endocrine systems thiab (4) cov neeg mob malignant qog. Txoj kev tshawb no tau pom zoo los ntawm pawg neeg saib xyuas kev coj ncaj ncees ntawm peb lub tsev kho mob (Affiliated Hospital of Nantong University, Affiliated Hospital of Jiangnan University, thiab Nanjing Medical University Affiliated Wuxi Second Hospital; 2013-K030), thiab tag nrho cov neeg mob lossis lawv tsev neeg tau cov ntaub ntawv qhia meej thiab kos npe rau daim ntawv pom zoo ua ntej kev kawm ua haujlwm.

Biochemical tsom xam

Txhua tus neeg mob yuav tsum tau yoo mov tsawg kawg yog 8 teev, thiab 3 mus rau 4 ml ntawm cov ntshav tau kos thiab xa mus rau chav kuaj mob hauv tsev kho mob. SCr, ntshav urea nitrogen (BUN), ntxiv C3, ntxiv C4 thiab immunoglobulins (g A, Ig M, Ig G) tau sim siv lub tshuab biochemical tsis siv neeg (Hitachi 7600, Hitachi, Tokyo, Nyiv). Tsis tas li ntawd, cov 24-teev cov zis protein tau ntsuas los ntawm immunoturbidimetry. Autoantibodies [anti-Smith (anti-Sm), anti-ob-stranded DNA (anti-dsDNA), anti-Sjögrens-syndrome-related antigen A (anti-SSA), anti-Sjogren's-syndrome-related antigen B (anti- SSB), tiv thaiv ribonucleoprotein (anti-RNP)] tau ntsuas los ntawm American Delrin BN-II tsis siv neeg immunoassay analyzer.

Kev Hloov Kho Kev Noj Qab Haus Huv Hauv Lub Raum Kab Mob 13 lub mis tau siv los suav cov eGFR raws li hauv qab no: eGFR=186 × SCr-1.154 × Hnub nyoog-020 × (0.742 hauv cov poj niam ). Cov txiaj ntsig tau qhia raws li mL·min-1(1.73m²)-1.

Ethylenediaminetetraacetic acid anticoagulated ntshav kuaj tau coj mus kuaj, thiab cov ntshav tau muab cais los ntawm centrifugation thiab khaws cia ntawm -20 degree . Txhawm rau kuaj xyuas sCD163, qhov kev tshuaj ntsuam xyuas enzyme-linked immunosorbent (ELISA) tau ua los ntawm kev siv tib neeg soluble CD163 ELISA Cov Khoom Siv (Kuv Biosource, San Diego, CA, USA) raws li cov chaw tsim khoom cov lus qhia. Kev kuaj pom ntau ntawm cov khoom siv no yog 1.56 txog 100ng / mL.

Kev tsom xam histological

Txhua tus neeg mob tau txais kev kuaj mob ultrasound-coj lub raum biopsy rau kev kuaj mob pathological. Kev faib tawm pathological ntawm LN tau ua tiav siv 2003 International Society of Nephrology/Renal Pathology Society (ISN/RPS) cov txheej txheem, thiab qhov ntsuas kev ua haujlwm (AI) thiab qhov ntsuas ntev ntev (CI) yog scored.14 AI suav nrog capillary endothelial hyperplasia, fibrillar-zoo li necro-sis, nuclear fragmentation, cellular crescents, hyaline thrombosis/platinum pob ntseg hloov, leukocyte infiltration, thiab/los yog interstitial inflammatory cell infiltration; CI muaj xws li glomerulosclerosis, fibrillar crescents, interstitial fibrosis, thiab lub raum tubular atrophy. Txhua qhov mob tau qhab nia 0 rau 3. AI yog 0 txog 24 cov ntsiab lus, thiab CI yog 0 txog 12 ntsiab lus.

Cov txheej txheem txheeb cais

Kev ntsuas kev faib tawm feem ntau zoo li qhov nruab nrab ± tus qauv qhia kev sib txawv (SD), t-tests tau siv rau kev sib piv pab pawg, thiab Pearson qhov kev tshuaj xyuas tau siv rau kev sib raug zoo. Cov ntaub ntawv uas tsis yog ib txwm muab faib tau qhia raws li qhov nruab nrab thiab quartiles [P50(P25, P75)], nrog rau Mann-Whitney U test rau pab pawg sib piv thiab Spearman qhov kev tshuaj xyuas rau kev sib raug zoo. Cov ntaub ntawv suav tau raug nthuav tawm raws li qhov zaus thiab tus nqi, thiab kev sib piv ntawm cov nqi tau ua los ntawm kev ntsuas lub voos xwmfab. Txoj kev Kaplan-Meier tau siv los ua cov phiaj xwm kev ciaj sia nyob rau hauv lub raum kev soj ntsuam kev muaj sia nyob, thiab kev ntsuas ntsuas ntsuas tau siv los sib piv cov nqi ciaj sia. IBM SPSS Statistics rau Windows, Version 19.0 (IBM Corp., Armonk, NY, USA) tau siv los ua cov ntaub ntawv, thiab P<0.05 was="" considered="" to="" indicate="" a="" statistically="" significant="">

Cov txiaj ntsig

Kev sib piv ntawm cov ntshav sCDl63 ntawm ob pawg

Hauv kev tshawb fawb tam sim no, peb tau txhais qhov ntsuas tus nqi ntau dua qhov nruab nrab ntxiv rau 2SD ntawm kev tswj hwm kev noj qab haus huv (69.7ng / mL) raws li qib siab sCD163 thiab faib 121 tus neeg mob nrog LN rau hauv ib pawg sCD163 ib txwm (72 tus neeg mob) thiab sCD163 siab. pab pawg (49 tus neeg mob). Serum sCD163 qib tau ntsuas los ntawm ELISA hauv txhua tus neeg mob nrog LN thiab 20 kev tswj hwm kev noj qab haus huv. sCD163 qib hauv pawg LN tau siab dua cov hauv pawg tswj hwm (67.04 ± 18.70 thiab 34.98 ± 17.49ng / mL, raws li), thiab qhov sib txawv yog qhov tseem ceeb (P<0.001)(figure>

Cov yam ntxwv ntawm tus neeg mob

Cov ntaub ntawv kho mob ntawm cov neeg mob Latin hauv ib txwm sCD163 pawg thiab pawg sCD163 siab tau qhia hauv Table 1. Txhua tus neeg koom nrog hnub nyoog 21 txog 54 xyoos. Lub hnub nyoog thiab poj niam txiv neej qhov sib txawv ntawm ob pawg tsis yog qhov tseem ceeb. Piv nrog rau cov neeg mob uas muaj LN nyob rau hauv ib txwm sCD163 pawg, cov neeg mob nyob rau hauv lub siab sCD163 pab pawg neeg tau nce siab 24- teev cov zis protein ntau, SCr, thiab BUN (tag nrho cov P.<0.05) and="" decreased=""><0.001). the="" sledai="" scores="" and="" positivity="" rates="" of="" c3,="" c4,="" iga,="" igm,="" igg,="" anti-dsdna,="" anti-sm,="" anti-ssa,="" anti-ssb,="" and="" anti-rnp="" were="" not="" statistically="" different="" between="" the="" two="">

Hauv pawg sCD163 siab, qhov kev kho mob glucocorticoid hauv paus yog 100 feem pua ​​​​, thiab qhov pib ntawm kev tiv thaiv kab mob tiv thaiv kab mob yog 95.91 feem pua, suav nrog 69.39 feem pua ​​​​ntawm cov neeg tau txais cyclophosphamide thiab 53.06 feem pua ​​​​uas tau txais hydroxychloroquine. Ntxiv rau, 48.98 feem pua ​​​​ntawm cov neeg mob tau siv ib qho tshuaj tiv thaiv kab mob hauv lub hauv paus, 46.94 feem pua ​​​​tau siv ob qho tshuaj tiv thaiv kab mob, thiab 1.63 feem pua ​​​​tau siv peb lossis ntau dua cov tshuaj tiv thaiv kab mob. Hauv cov pab pawg sCD163 ib txwm muaj, qhov kev kho mob glucocorticoid hauv paus yog 100 feem pua ​​​​, thiab qhov pib ntawm kev tiv thaiv kab mob tiv thaiv kab mob yog 93.88 feem pua, suav nrog 65.31 feem pua ​​​​ntawm cov neeg tau txais cyclophosphamide thiab 55.10 feem pua ​​​​uas tau txais hydroxychloroquine. Ntxiv mus, 53.06 feem pua ​​​​ntawm cov neeg mob tau kho nrog ib tus neeg tiv thaiv kab mob, 42. Tsis muaj qhov sib txawv tseem ceeb hauv cov nqi hauv qab ntawm ob pawg.

Lub raum pathological qib

Lub raum pathological kev faib tawm ntawm txhua tus neeg mob nrog LN tau txais los ntawm lub raum biopsy, thiab raws li kev faib tawm pathological ntawm LN tsim los ntawm ISN / RPS hauv xyoo 2018, l4 cov neeg mob nrog LN tau muab faib ua rau qib: qib qis (I), mesenteric. hyperplasia qib (II), focal segmental qib (III), diffuse hyperplasia qib (IV), qib membranous (V) thiab qib sclerosis (VI). Vim tias tus lej ntawm LN nrog V ntxiv rau III (peb kis) thiab V ntxiv rau IV (ob kis) hauv txoj kev tshawb no me me, ob qib tau muab faib ua qib V. Kev faib tawm ntawm qib I, Ⅱ, III, IV, thiab Vin pawg sCD163 siab thiab pab pawg sCD163 ib txwm muaj nyob hauv daim duab 2. Cov neeg mob uas muaj LN nyob rau hauv pawg sCD163 siab tau muaj qib pathological ntau dua piv rau cov neeg hauv pawg sCD163 ib txwm, thiab qhov sib txawv yog qhov tseem ceeb (Mann-Whitney U tus nqi{ {8}}, P=0.007).

Cov qhab nia ntawm lub raum pathology

AI thiab CI cov qhab nia ntawm lub raum mob hauv cov neeg mob nrog LN tau txheeb xyuas los ntawm ib txoj hauv kev ib nrab. AI tau qhab nia 0 rau 24 ntsiab lus, thiab CI tau qhab nia 0 -12 cov qhab nia. AI thiab CI cov qhab nia ntawm cov neeg mob siab sCD163 qib tau nce ntxiv piv rau cov neeg mob uas muaj qib sCD163. Qhov sib txawv yog qhov tseem ceeb (tag nrho P<0.05), as="" shown="" in="" figure="">

Kev sib raug zoo ntawm cov ntshav sCDI63 thiab lub raum raug mob qhov ntsuas ntsuas hauv cov neeg mob LN

Kev raug mob raum raug soj ntsuam los ntawm SCr, BUN, eGFR, thiab cov cim qhia txog kab mob, nrog rau cov qhab nia ntawm cov kab mob pathological thiab lub raum pathology. Kev txheeb xyuas kev sib raug zoo tau pom tias qib sCD163 hauv cov neeg mob LN tau muaj txiaj ntsig zoo nrog SCr(r=0.521, P<0.01),bun (r=""><0.01),ai (r=""><0.01) and="" ci(r="0.611,"><0.01) and="" negatively="" correlated="" with="" egfr="" (r=""><0.01), as="" shown="" in="" figure="">

sCD163 ua tus cim kev kwv yees ntawm lub raum qhov kawg ntawm cov xwm txheej

Renal endpoints in this study were defined as the occurrence of ESRD, eGFR reduction >50% or >2-fold nce hauv SCr.Tom qab qhov kev soj ntsuam zoo li 25 (10,34) lub hlis, yim tus neeg mob tau pom tias muaj 2-fold nce hauv SCr, thiab peb tus neeg mob tau tsim ESRD. Lub raum ciaj sia taus nkhaus ntawm cov neeg mob nyob rau hauv siab sCD163 pab pawg neeg thiab ib txwm sCD163 pawg yog qhia nyob rau hauv daim duab 5. Lub raum ciaj sia taus tus nqi ntawm cov neeg mob nyob rau hauv lub siab sCD163 pab pawg neeg yog qis dua li qhov qub sCD163 pawg (85.71 feem pua ​​vs 94.44 feem pua, feem, P=0.017) (Daim duab 5).

Kev sib tham

Transmembrane glycoprotein CD163, ib tug scavenger receptor qhia nyob rau hauv monocytes thiab macrophages, yog ib tug M2 macrophage serum marker.In vivo, CD163 muaj nyob rau hauv ob daim ntawv: ib tug transmembrane macromolecule ntawm mononuclear / macrophage mem-branes [CD16CD3) thiab ntshav lossis lwm cov kua dej.sCD163 yog cov khoom tso tawm ntawm mCD163. Qhov kev qhia ntawm CD163 yog cuam tshuam los ntawm ntau yam. Piv txwv li, interleukin6 (IL6), IL10, thiab glucocorticoids txhawb kev qhia ntawm CD163, thaum qog necrosis factor-a, interferon-y (IFN-y), thiab platelet factor 4 inhibit qhov kev qhia ntawm CD163. Thaum cov lus teb mob hnyav thiab mob ntev, CD163 raug tso tawm los ntawm qhov chaw ua haujlwm macrophages thiab tso rau hauv cov ntshav peripheral hauv daim ntawv soluble. Yog li, ntshav sCD163 yog ib qho cim ntawm kev ua kom macrophage.

Hauv txoj kev tshawb fawb tam sim no, cov ntshav sCD163 tau ntsuas hauv cov qauv ntshav peripheral los ntawm ELISA thiab pom tias muaj ntau dua rau cov neeg mob LN ntau dua li kev tswj hwm kev noj qab haus huv, uas zoo ib yam nrog cov kev tshawb fawb los ntawm Nakayama li al.9 thiab Zizzo li al.10 Tsis ntev los no cov kev tshawb fawbl6 . Kuv tau tshaj tawm tsis tau tsuas yog nce qib sCD163 hauv cov neeg mob SLE tab sis kuj tseem ua rau cov tawv nqaij thiab lub raum CD163 ntxiv rau macrophage infiltration, thiab cov kws tshawb fawb tau hais tias CD163 tuaj yeem koom nrog hauv pathogenesis ntawm SLE. Peb pom tias cov ntshav sCD163 tau nce siab hauv cov neeg mob LN, qhia tias kev ua kom macrophage koom nrog kev txhim kho LN thiab sCD163 tuaj yeem ua tus neeg kho mob inflammatory hauv cov txheej txheem immunopathogenic ntawm LN. Macrophages tuaj yeem koom nrog hauv pathogenesis ntawm LN los ntawm cov txheej txheem hauv qab no. Ua ntej, macrophages qhia cov cim tshwj xeeb ntawm lawv qhov chaw, paub txog ib puag ncig ib puag ncig, thiab teb rau endogenous thiab exogenous stimuli los cuam tshuam lub cev tiv thaiv kab mob thiab koom nrog hauv pathogenesis ntawm LN. Thib ob, dysregulated cytokine secretion aberrantly activates thiab txhim khu lub adaptive tiv thaiv kab mob. Macrophages txhawb T- thiab B-cell activation thiab yog lub hauv paus tseem ceeb ntawm cov cytokines, xws li IL-6 thiab IL{12}}, uas txhawb nqa IgG thiab IFN{13}} ntau lawm, yog li inducing B cell. kev sib txawv rau hauv cov tshuaj tiv thaiv-secreting plasma cells. Tsis tas li ntawd, B-cell activating factor feem ntau yog los ntawm macrophages thiab txhawb nqa B-cell ciaj sia thiab proliferation.20 Thib peb, kev ua haujlwm tsis zoo hauv macrophage phagocytosis thiab pathogen clearance thiab apoptotic thiab necrotic hlwb yuav muab autoantigens rau txoj kev loj hlob ntawm LN. Qhov tsis txaus ntseeg tshem tawm los ntawm macrophages hauv cov neeg mob nrog LN tuaj yeem cuam tshuam nrog kev txo qis ntawm CD44 ntawm qhov chaw ntawm macrophages, txo qhov sib txawv ntawm CD34 ntxiv rau hematopoietic qia hlwb rau hauv macrophages paub tab thiab txo qis qib ntxiv. Yog li, macrophage dysfunction plays lub luag haujlwm tseem ceeb hauv cov kab mob ntawm LN, thiab cov txheej txheem tshwj xeeb yuav tsum tau tshawb xyuas ntxiv. Peb txoj kev tshawb nrhiav pom tias cov neeg mob LN hauv cov pab pawg sCD163 siab tau nce SCr thiab BUN thiab txo eGFRs piv nrog cov neeg hauv pawg sCD163. Tsis tas li ntawd, peb pom tias yim tus neeg mob tau pom tias muaj 2-pw nce hauv SCr, thiab peb tus neeg mob tau tsim ESRD. Cov txiaj ntsig no tau qhia tias qib sCD163 muaj kev cuam tshuam rau lub raum kev puas tsuaj loj hauv LN, qhia tias cov ntshav sCD163 yuav tsim nyog rau kev ntsuam xyuas ntawm lub raum puas tsuaj loj hauv LN. Cov protein tso zis tsis tsuas yog qhia txog qhov muaj cov kab mob glomerular nyob rau hauv cov neeg mob uas muaj LN tab sis kuj muaj kev cuam tshuam ncaj qha rau lub raum mesangial hlwb thiab tubules, uas ua rau muaj kev sib txuam ntawm extracellular matrix thiab ua rau lub raum puas tsuaj. Peb pom tias 24-teev cov zis protein ntau hauv cov pab pawg sCD163 siab tau nce ntau dua piv nrog rau hauv pawg sCD163 ib txwm. Cov qhab nia SLEDAI feem ntau yog siv hauv tsev kho mob los ntsuas cov kab mob SLE, thiab nrog kev nce qib ntawm SLEDAI cov qhab nia, qib kev ua haujlwm ntawm lupus nce zuj zus.14 Peb pom tsis muaj qhov sib txawv tseem ceeb hauv SLEDAI cov qhab nia nruab nrab ntawm pawg sCD163 siab thiab pawg sCD163. Qhov no tej zaum yuav yog vim muaj ntau yam tseem ceeb suav nrog hauv SLEDAI cov qhab nia xeem, thiab lub raum cov ntsiab lus suav nrog ib feem me me. Cov txiaj ntsig ntawm peb txoj kev tshawb fawb tau zoo ib yam li Nakayama et al.9 Hauv qhov sib piv, Zizzo et al.10 pom tias cov ntshav sCD163 tau muaj txiaj ntsig zoo nrog cov qhab nia SLEDAI hauv 40 cov neeg mob SLE. Activated macrophages qhia CD163(M2) yog cov subtypes ntau tshaj ntawm macrophages hauv lub raum biopsies los ntawm cov neeg mob LN. Cov kev tshawb fawb2 tau pom tias cov zis sCD163 qib tau nce siab hauv cov neeg mob uas muaj LN nquag thiab cuam tshuam nrog rau cov qhab nia SLEDAI. Kev txo qis hauv cov zis sCD163 tom qab kev kho mob tuaj yeem pab saib xyuas cov lus teb rau kev kho hauv LN. Raws li cov tshuaj tiv thaiv tshwj xeeb rau SLE, nce titers ntawm anti-dsDNA cov tshuaj tiv thaiv kab mob cuam tshuam nrog LN kev ua, thiab cov tshuaj tiv thaiv dsDNA tuaj yeem siv los ua ib qho kev pheej hmoo rau LN. Anti-RNP cov tshuaj tiv thaiv yog ib qho cim ntawm cov kab mob sib xyaw ua ke thiab muaj kev sib raug zoo nrog ntau yam kev kho mob ntawm cov kab mob autoimmune, xws li SLE, Sjogren's syndrome, thiab systemic sclerosis.14 Peb pom tias txawm tias cov pab pawg sCD163 siab tau nce qhov txiaj ntsig zoo. anti-dsDNA cov tshuaj tiv thaiv thiab txo cov txiaj ntsig zoo ntawm cov tshuaj tiv thaiv RNP piv nrog cov pab pawg sCD163 ib txwm, qhov sib txawv ntawm ob tsis yog qhov tseem ceeb. Cov kev tshawb pom no qhia tias qib sCD163 siab muaj qhov cuam tshuam tsawg kawg nkaus ntawm qhov zoo ntawm autoantibodies thiab tias ob leeg tuaj yeem ua lub luag haujlwm ywj pheej hauv kev txhawb nqa kev loj hlob ntawm LN.

LN pathological kev faib tawm thiab cov qhab nia rau lub raum pathology yog qhov tseem ceeb rau kev kuaj mob, kev kho mob, thiab kev kwv yees. AI nyob rau hauv lub raum pathology qhab nia yog ze ze rau kev kho mob tshwm sim thiab feem ntau qhia txog cov theem ntawm mob inflammatory kev puas tsuaj rau lub raum cov ntaub so ntswg. CI yog proportional rau prognosis thiab sawv cev rau qib ntawm lub raum puas. Ib txoj kev tshawb fawb ntawm 1814 Suav cov neeg mob nrog LN txheeb xyuas cov kab mob ntawm lub raum biopsy uas yog qhov muaj feem pheej hmoo rau ESRD.26Nyob rau hauv txoj kev tshawb no, peb tau soj ntsuam cov ntaub ntawv rau lub raum pathological ntawm 121 cov neeg mob uas muaj LN thiab xaus lus tias kev faib tawm pathological, AI cov qhab nia, thiab CI. Cov qhab nia ntawm cov neeg mob hauv cov pab pawg sCD163 siab tau txawv ntawm cov neeg nyob hauv ib txwm sCD163 pawg, thiab kev tshuaj xyuas ntxiv tau qhia tias muaj kev sib raug zoo ntawm cov ntshav sCD163 thiab AI thiab CI cov qhab nia. Qhov txiaj ntsig no qhia ntxiv tias qhov nce qib sCD163 hauv cov ntshav tuaj yeem cuam tshuam nrog qhov mob ntawm lub raum raug mob thiab tsis zoo rau cov neeg mob LN.

Hauv kev xaus, sCD163 tuaj yeem koom nrog hauv cov txheej txheem immunopathological ntawm LN ua tus neeg kho mob inflammatory. Cov qib sCD163 cuam tshuam nrog qhov hnyav ntawm LN thiab yog ib qho tseem ceeb qhia txog kev mob raum tsis zoo hauv cov neeg mob LN, qhia tias kev ua kom macrophage koom nrog hauv kev txhim kho LN. Serum sCD163 qhia tau tias muaj peev xwm ua tau zoo bio-logical marker rau LN kev kuaj mob, kev kuaj mob sib txawv, thiab kev soj ntsuam kev soj ntsuam thiab muab lub hom phiaj kho tshiab los txo qis kev mob thiab txhim kho kev mob.