Cov txiaj ntsig ntawm Tag Nrho Glycosides Ntawm Cistanche Ntawm Kev Kawm Thiab Kev Ua Tsis Txaus Ntshai hauv Cov nas

Gao Cong 1, Wang Chuanshe 2, Wu Guanzhong 1, Tu Pengfei 2 (1. Pharmacology Teaching and Research Office of China Pharmaceutical University, Nanjing 210009; 2. Medical Department of Peking University, Beijing 100083)

Abstract: Lub Hom Phiaj Saib xyuas cov txiaj ntsig ntawm tag nrho glycosides ntawmCistanche deserticolantawm scopolamine-induced kev kawm thiab nco acquisition disorder thiab sodium nitrite-induced kawm thiab nco consolidation teeb meem nyob rau hauv nas. Methods Cov kev kawm thiab kev nco ua haujlwm ntawm cov nas tau pom los ntawm cov txheej txheem nqis-down. Tom qab kev tswj hwm intragastric tas li ntawm tag nrho glycosides ntawm Cistanche deserticola rau 30 hnub, cov tsiaj tau kawm nyob rau hnub 29 ntawm qhov kev sim thiab sim rau hnub 30. Lub latency ntawm thawj kauj ruam thiab tus naj npawb ntawm qhov yuam kev hauv 5 feeb tau kaw. Tus qauv scopolamine tau txhaj tshuaj intraperitoneally 15 feeb ua ntej kev cob qhia, thaum tus qauv sodium nitrite tau txhaj subcutaneously tom qab kev cob qhia. Cov txiaj ntsig Piv nrog rau pawg qauv scopolamine, lub sijhawm incubation ntawm tag nrho cov glycosides ntawm cistanche 400, 200 mg / kg pawg thiab Naofukang pawg tau ntev heev, thiab tus lej ntawm qhov ua yuam kev tau txo qis; Lub sijhawm incubation ntawm tag nrho glycosides ntawmCistanche deserticola100mg / kg pawg kuj tau ntev heev, tab sis tus naj npawb ntawm kev ua yuam kev tsuas yog txo qis rau qee yam. Piv nrog rau cov qauv sodium nitrite, lub sij hawm incubation ntawm tag nrho cov glycosides ntawm cistanche 400 thiab 200 mg / kg pawg tau ntev heev, thiab tus naj npawb ntawm cov kev ua yuam kev txo qis rau qee qhov, tab sis tsis muaj qhov tseem ceeb ntawm kev txheeb cais; Hauv tag nrho glycosides ntawm cistanche 100mg / kg pawg, latency thiab tus lej ntawm qhov yuam kev tsuas yog txhim kho rau qee yam; Lub latency ntawm Naofukang pab pawg tau ntev heev, thiab tus lej ntawm qhov yuam kev tau txo qis.Kev xaus tag nrho cov glycosides ntawm Cistanche tuaj yeem txhim kho kev kawm thiab kev nco tau tsis zoo uas tshwm sim los ntawm scopolamine thiab kev kawm thiab kev nco txog kev sib koom ua ke los ntawm sodium nitrite hauv cov nas.

Cistanche deserticola - Txhim kho kev nco

Nyem qhov no mus saib Cistanche Txhim Kho Cov Khoom Siv Nco

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Cov lus tseem ceeb: tag nrho glycosides ntawmcistanche deserticola; Nas; Kev kawm thiab kev nco; Txoj kev dhia platform

Suav Library Classification No.: R285.5    Cov ntaub ntawv ID: A   Tshooj No.: 1003-9783 (2005) 03-0162-03

Cov neeg laus tau tshawb nrhiav cov tshuaj muaj kev nyab xeeb thiab muaj txiaj ntsig zoo rau kev tiv thaiv thiab kev kho mob Alzheimer's qhov chaw kub hauv kev tshawb fawb tshuaj. Nyob rau hauv xyoo tas los no, Suav cov tshuaj tau ua tau zoo nyob rau hauv kev kho mob ntawmAlzheimer tus kab mobnrog rau cov tshuaj uas feem ntau invigorate lub raum thiab cov hlab ntsha thiab kuj invigorate lub plawv thiab spleen.Cistanche deserticola kuv, Cov tshuaj suav tshuaj, yog thawj zaug luam tawm hauv Shennong's Classic of Materia Medica thiab muaj cov teebmeem ntawm tonifying raum yang, muaj txiaj ntsig zoo rau cov ntshav, moistening hnyuv, thiab txo cov cem quav. Nws yog tam sim no dav siv los kho senile dementia, impotence, ib txwm cem quav, thiab lwm yam kab mob [1,2]; Cov kev tshawb fawb tau pom tias nws muaj cov teebmeem ntawm kev tiv thaiv kev puas hlwb, tiv thaiv oxidation, tiv thaiv hypoxia, tiv thaiv hluav taws xob thiab txhim kho kev tiv thaiv kab mob; Lub ntsiab tivthaiv yog phenylethanolamine glycosides[3-8]. Tag nrho glycosides ntawm Cistanche deserticola yog muab rho tawm los ntawm qhuav nqaij stems ntawm Cistanche deserticola (Schenk) ci, muaj 90.7 feem pua ​​​​ntawm phenylethanoid glycosides thiab 44.3 feem pua ​​​​ntawm cov ntsiab lus tseem ceeb echinacoside.Hauv daim ntawv no, cov nyhuv ntawm tag nrho glycosides ntawmcistanche deserticolantawm kev kawm thiab kev nco tsis zoo ntawm cov nas uas tshwm sim los ntawm scopolamine thiab sodium nitrite tau kawm los muab pov thawj rau nws txoj kev loj hlob raws li cov tshuaj tshiab los txhawb kev txawj ntse thiab tiv thaiv thiab kho Alzheimer's disease.

Cistanche ua dab tsi - tiv thaiv Alzheimer's kab mob

1 .Cov khoom siv thiab cov txheej txheem

1.1 Tsiaj

180 noj qab nyob zoo Kunming nas, hnyav 18 ~ 22g, ib nrab txiv neej thiab ib nrab poj niam, tau muab los ntawm Tsiaj Center ntawm Tuam Tshoj Pharmaceutical University, nrog rau daim ntawv pov thawj naj npawb Su Dong (Zhi) 97004.

1.2 Tshuaj thiab tshuaj reagents

Tag nrho glycosides ntawmcistanchedeserticola, muab los ntawm Chemistry Department ntawm Modern Research Center of Traditional Chinese Medicine, Peking University, nrog batch tooj 980601. Naofukang ntsiav tshuaj, tsim los ntawm Yixing Pharmaceutical Factory, batch tooj 980521. Scopolamine txhaj, tsim los ntawm Shanghai Hefeng Pharmaceutical Co., Ltd. , batch naj npawb 980101. Sodium nitrite, tsim los ntawm Rugao Huarui Reagent Factory, batch naj npawb 980220. Cov tshuaj thiab reagents saum toj no tau npaj nrog dej distilled mus rau qhov xav tau ntawm kev sim ua ntej siv.

Cov txiaj ntsig ntawm cistanche tubulosa -tiv thaiv Alzheimer's disease

1.3 Kev ntsuas

Lub wx-Z nas platform dhia seev cev yog tsim los ntawm lub koom haum ntawm Tshuaj, Suav Academy ntawm Kev Kho Mob Sciences.

1.4 Method

1.4.1 Replication and test of scopolamine-induced memory acquisition disorder model nyob rau hauv mdej khov [9]

Raws li lub cev hnyav, cov nas tau muab faib ua pawg ib txwm, pawg qauv, pawg Naofukang, thiab siab, nruab nrab, thiab qis pawg ntawm tag nrho glycosides ntawm cistanche, nrog 15 nas nyob rau hauv txhua pab. Cov pab pawg ib txwm thiab cov qauv tau muab tib lub ntim ntawm cov dej distilled los ntawm gavage ib hnub rau 30 hnub.

Lub platform dhia dej kev cob qhia tau ua rau hnub 29 ntawm qhov kev sim. Cov tshuaj tau siv los ntawm gavage 1 teev ua ntej kev cob qhia, thiab scopolamine 1 mg / kg tau txhaj tshuaj intraperitoneally hauv txhua pab pawg tshwj tsis yog pawg tswj hwm ib txwm 15 feeb ua ntej kev cob qhia. Thaum lub sij hawm kev cob qhia, muab cov nas tso rau hauv lub thawv tshuaj tiv thaiv ntawm lub platform tester kom hloov mus rau ib puag ncig rau 3min, thiab tom qab ntawd tam sim ntawd siv 36V alternating tam sim no. Tom qab tau txais hluav taws xob poob siab, feem ntau ntawm cov nas dhia mus rau lub platform kom dim ntawm hluav taws xob poob siab. Tom qab dhia tawm ntawm lub platform thiab tau txais hluav taws xob poob siab, cov nas dhia rov qab mus rau lub platform rau 5 feeb. Tom qab 24 teev, muab tus nas tso rau ntawm lub platform thiab sau lub sijhawm (latency) ntawm thawj zaug tus nas dhia tawm ntawm lub platform thiab tus naj npawb ntawm hluav taws xob shocks (tus lej yuam kev) hauv 5min.

1.4.2 Luam thiab ntsuam xyuas cov qauv kev nco txog kev sib koom ua ke hauv cov nas uas tshwm sim los ntawm sodium nitrite [9]

Kev sib koom ua ke, kev tswj hwm, kev cob qhia, thiab kev sim yog tib yam li ua ntej, tab sis scopolamine tsis tau txhaj tshuaj intraperitoneally 15 feeb ua ntej kev cob qhia, tab sis 120mg / kg sodium nitrite tau txhaj subcutaneously tom qab kev cob qhia.

1.5 Kev ua lej

Cov txiaj ntsig ntawm latency thiab tus naj npawb ntawm qhov yuam kev hauv 5 min tau qhia hauv "x ± s", thiab cov ntaub ntawv raug txheeb xyuas los ntawm kev sib koom ua ke t-test.

2.Cov txiaj ntsig

2.1 Qhov cuam tshuam rau kev kawm scopolamine-induced kev kawm thiab kev nco tsis zoo hauv cov nas

Piv nrog rau cov pab pawg ib txwm muaj, qhov latency ntawm cov kauj ruam-down xeem hauv pawg qauv tau luv luv thiab cov lej ntawm qhov yuam kev tau nce ntxiv, qhia tau tias kev kawm thiab kev nco tau ntawm cov nas yog qhov tsis zoo. Piv nrog rau pawg qauv, qhov latency ntawm qhov kev sim hauv Naofukang pab pawg thiab cov koob tshuaj ntau thiab nruab nrab ntawm tag nrho cov glycosides ntawm cistanche tau ntev heev, thiab tus lej ntawm qhov ua yuam kev tau txo qis (P<0.01); The test latency of mice in the low dose group of total glycosides of cistanche was also significantly prolonged (P<0.01); The number of test errors decreased to a certain extent, but there was no significant difference compared with the model group. The results showed that the total glycosides of cistanche could improve the learning and memory impairment of mice induced by scopolamine, and the action intensity of the high and middle-dose groups was better than that of the Naofukang group. The results are shown in Table 1.

Table 1 Cov nyhuv ntawm tag nrho glycosides ntawm cistanche ntawm scopolamine-vim kev kawm thiab kev nco tsis zoo hauv nas (x ± s)

Lus Cim: Piv nrog rau pawg qauv, * P<0.05, * * P<0.01. () is the number of deaths from electric shock, the same below.

2.2 Qhov cuam tshuam rau kev kawm thiab kev nco txog kev sib koom ua ke ntawm cov nas uas tshwm sim los ntawm sodium nitrite

Lub latency ntawm cov kauj ruam-down xeem ntawm cov qauv pab pawg nas tau luv luv, thiab tus naj npawb ntawm cov kev xeem yuam kev tau nce, uas yog qhov txawv ntawm cov pab pawg ib txwm, qhia tias cov qauv pab pawg nas muaj teeb meem hauv kev kawm thiab kev nco ua ke. Piv nrog rau pawg qauv, qhov ntsuas latency ntawm cov nas nyob rau hauv cov koob tshuaj siab thiab nruab nrab ntawm tag nrho glycosides ntawm cistanche tau ntev heev (P<0.05~0.01); The number of errors decreased to a certain extent, but there was no statistical significance; The latency and the number of errors in the test of mice in the low dose group of total glycosides of cistanche also improved to some extent, but there was no statistical significance; In Naofukang group, the latency of test was significantly prolonged and the number of test errors was significantly reduced (P<0.01). The results of this experiment show that the oral administration of high and medium doses of total glycosides of cistanche can significantly improve the learning and memory consolidation disorder of mice caused by sodium nitrite, but it is weaker than Naofukang. The results are shown in Table 2.

Table 2 Cov nyhuv ntawm tag nrho cov glycosides ntawm cistanche ntawm kev kawm thiab kev nco kev sib koom ua ke hauv cov nas vim yog sodium nitrite (x ± s)

Lus Cim: Piv nrog rau pawg qauv, * P<0.05, * * P<0.01.

3.Kev sib tham

Kev kawm thiab kev nco yog qhov ua haujlwm siab heev ntawm lub hlwb thiab cov ntsiab lus ntawm kev txawj ntse.

Superman tshuaj ntsuab cistanche—pkho tus kab mob Alzheimer

Tag nrho cov haujlwm neural ntawm kev kawm thiab kev nco tuaj yeem muab faib ua peb yam txheej txheem neural: nrhiav tau, sib sau ua ke, thiab kev tsim tawm. Kev kawm thiab kev nco tsis zoo yog cov tsos mob ntawm ntau yam kab mob hauv lub hlwb, suav nrog cerebral vascular sclerosis, cerebral blood insufficiency, thiab dementia hauv cov neeg laus.

Cov tshuaj suav tshuaj ntseeg hais tias Alzheimer's tus kab mob feem ntau yog ib qho tsis muaj peev xwm. Lub raum tsis txaus thiab tsis muaj peev xwm ntawm lub plawv thiab spleen ua rau lub cev tsis muaj dej hiav txwv, uas ua rau muaj kev ntxhov siab."Dej hiav txwv ginseng"Cistanche deserticolayog ib qho tshuaj tonic thiab tshuaj aphrodisiac, nrog rau kev ua haujlwm ntawm tonifying lub raum, sau cov ntsiab lus, thiab moistening cov hnyuv. Classic ntawm Materia Medica hu nws cov tshuaj tseem ceeb rau tonifying cov ntshav essence thiab nourishing lub raum. Nws muaj txiaj ntsig zoo rau cov tsos mob ntawm qaug zog, tsis nco qab, tsis hnov ​​​​lus, thiab enuresis tshwm sim los ntawm lub raum tsis txaus.Zhu Zhiming thiab lwm tus tau txheeb xyuas cov tshuaj muaj pes tsawg leeg ntawm 46 lub tebchaw rau kev kho mob senile dementia luam tawm hauv cov ntawv xov xwm cuam tshuam los ntawm 1990 txog 1999 thiab pom tias Cistanche deserticola yog ib qho tshuaj siv ntau tshaj plaws [1].

Cistanche ntxiv nyob ze kuv- Txhim kho kev nco

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Kev sim tsiaj ntawm Sato Chow hauv Nyij Pooj tau lees paub tias Cistanche deserticola muaj kev tiv thaiv dementia [3]. Peb cov kev sim yav dhau los tau pom tias tag nrho glycosides ntawm Cistanche deserticola tau txhawb nqa kev kawm thiab kev nco ua haujlwm ntawm cov nas ib txwm muaj, thiab tiv thaiv kev kawm thiab kev nco ua haujlwm ntawm ethanol-induced kev kawm thiab nco kev tsim tawm tsis zoo qauv nas, raum-yang deficiency qauv nas, vascular. dementia qauv nas, thiab lwm yam. Ua ke nrog cov txiaj ntsig ntawm qhov kev sim no, nws tau lees paub tias Cistanche deserticola muaj cov txiaj ntsig ntawm kev txhawb kev txawj ntse, thiab muaj kev tiv thaiv zoo rau kev nrhiav tau, sib sau ua ke, thiab kev tsim tawm ntawm peb qhov kev ua haujlwm neural hauv tag nrho cov haujlwm neural. kev kawm thiab kev nco,uas qhia tau hais tias cov nyhuv ntawmCistanche deserticolaglycosides ntawm kev txhawb kev txawj ntse yog ntau theem thiab ntau qhov txuas. Yog li ntawd, tag nrho glycosides ntawm cistanche yuav dhau los ua ib qho tshuaj zoo rau kev kho mobAlzheimer tus kab mobthiab lwm yam kab mob nrogkev kawm thiab kev ncomob raws li cov tsos mob tseem ceeb.

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