Cov teebmeem Ntawm Cov Extracted Txawv Ntawm Cistanche Deserticola Ntawm Hypoglycemic Effects Ntawm Db / Db Dub nas Ⅲ

3 Kev sim cov txiaj ntsig

3.1 Cov ntsiab lus kev txiav txim siab ntawm cov khoom sib txawv ntawm Cistanche deserticola / Cistanche deserticola

Ntsuas raws li cov xwm txheej hauv qab "2.3", cov ntsiab lus kev txiav txim siab ntawm cov khoom sib txawv ntawm Cistanche deserticola thiabTartschula deserticolatau qhia hauv Table 1.

NATURAL ORGANIC CISTANCHE EXTRACT nrog 30% ECHINACOSIDE thiab 12% ACTEOSIDE

3.2 Pharmacological Performance index ntsuas ntsuas

3.2.1 Kev soj ntsuam dav dav

Lub sijhawm sim tsiaj yog 4 lub lis piam, thiab lub cev ceev ceev ntawm cov nas dub tau ntsuas txhua lub lis piam. Cov txiaj ntsig tau pom nyob rau hauv Table 2. Txij thaum pib mus rau qhov kawg ntawm qhov kev sim gavage, qhov hnyav ntawm cov nas dub hauv pawg qauv tau nce ntxiv, uas tau ua raws li cov yam ntxwv ntawm qhov hnyav nce hauv cov qauv ntshav qab zib hom 2. Qhov hnyav ntawm nas nyob rau hauv pawg tswj hwm ib txwm tseem tsis tau hloov pauv. Tom qab 1 lub lis piam ntawm kev tswj hwm intragastric ntawm db / db cov nas dub hauv ntau pawg tswj hwm, piv nrog rau pawg qauv, qhov hnyav nce ntawm nas nyob rau hauv txhua pawg tswj hwm tau txhim kho mus rau qhov sib txawv, ntawm qhov kev txhim kho ntawm tag nrho glycosides ntawm tartrum yog cov loj tshaj. Qhov tseem ceeb (P<0.05); after 2 weeks and 4 weeks of intragastric administration of db/db black mice in different administration groups, the body weight of Amaranth oligosaccharide improved significantly (P<0.05 or P<0.01) . The above results show that different extracted parts of Cistanche deserticola and Branco deserticola have the effect of reducing the body weight of db / db black mice. The results are shown in Table 2.

3.2.2 Cov txiaj ntsig ntawm qhov sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola ntawm cov ntshav qabzib theem ntawm db/db cov nas dub

Cov ntshav qab zib ntawm cov nas dub db / db hauv pawg tswj hwm ib txwm muaj nyob rau hauv ib txwm muaj (4.3 ~ 5.7 mmol·L-1), thaum cov ntshav qab zib ntawm cov nas dub hauv pawg qauv txuas ntxiv nce ntxiv. (P<0.01). Compared with the model group, after 1 week of administration, the total polysaccharides, total oligosaccharides, and total glycosides of Cistanche deserticola group and the total polysaccharides, total oligosaccharides, and total glycosides FBG of Tartistan camphora group were reduced (P<0.01); after 2 weeks of administration, After 3 weeks of administration, the total glycosides FBG of tartschistanches were reduced (P＜0.05); after 3 weeks of administration, the total polysaccharides and total oligosaccharides FBG of tartans were reduced (P＜0.05 or P＜0.01); After taking the medicine for 4 weeks, the total polysaccharides, total oligosaccharides of Cistanche deserticola, and the total polysaccharides, total oligosaccharides, and total glycosides FBG of Cistanche deserticola were reduced (P <0.05). The results are shown in Table 3.

Hauv kev kuaj ntshav qabzib hauv qhov ncauj (saib Daim duab 4), qhov nruab nrab cov ntshav qabzib qib ntawm cov pab pawg tswj hwm ntawm cov nas dub tau mus txog qhov siab kawg ntawm 30 feeb, thiab tom qab ntawd poob sai sai mus rau qib qub. Txawm li cas los xij, cov ntshav qabzib nruab nrab ntawm cov nas dub hauv pawg qauv txuas ntxiv nyob rau theem siab tom qab mus txog qhov kawg ntawm 30 feeb. Piv nrog rau cov qauv pab pawg, cov ntshav qabzib ntawm cov nas dub hauv txhua pawg tswj hwm tau pom qhov kev poob qis mus rau qib sib txawv tom qab ncav cuag tus nqi siab tshaj, ntawm cov uas tag nrho cov polysaccharide, tag nrho cov oligosaccharide thiab tag nrho glycosides pawg ntawm Tartschalna campestris pawg poob sai dua. Tsis tas li ntawd, raws li kev suav AUC ntawm txhua pab pawg, cov txiaj ntsig tau pom tias AUC ntawm cov nas dub hauv txhua pawg tswj hwm qis dua li ntawm pawg qauv, thiab tag nrho cov polysaccharides ntawm Cistanche deserticola thiab tag nrho glycosides ntawm Tartistan deserticola txo qis. AUC ntawm cov nas dub ntawm 2 lub sijhawm cov ntsiab lus (90 thiab 120 min) tom qab tswj hwm cov piam thaj.Ntshav qab zib tus nqi(P<0.05 or P<0.01), total oligosaccharides of Cistanche deserticola and total polysaccharides of Tartschalistxo cov ntshav qabzibtus nqi tom qab noj cov piam thaj rau 120 feeb (P<0.05), total oligosaccharides of Tartschalis. Ittxo peb lub sij hawm cov ntsiab lus(0, 90, thiab 120 feeb) tom qab kev tswj hwm cov piam thaj thiab thaj chaw hauv qab nkhaus (P<0.05 or P<0.01). The results showed that Cistanche deserticola and tartaric acid deserticola could improve the OGTT ability of db / db black mice.

Piv nrog rau ib txwm tswj pab pawg, lubinsulin tsis kam index(HOMA-IR) thiabQib HbA1c ntawm cov nas dubhauv pawg qauv nce (P<0.05), and the pancreatic islet β cell secretion index (HOMA-β) level decreased. Compared with the model group, the serum HbA1c content of the Cistanche deserticola oligosaccharide group, Cistanche deserticola polysaccharide group, Tartschistanche deserticola oligosaccharide group and Tartschistanche deserticola total glycosides group was significantly reduced (P <0.05); , the HOMA-IR index of the alcoholic acid agaricus oligosaccharide group and the alcoholic acid agaricus total glycosides group was significantly reduced (P <0.05); after 4 weeks of intervention in the administration group, compared with the model group, the black mice in each administration group The secretion index levels of pancreatic β-cells increased to varying degrees. The results are shown in Table 5.

Insulin yog cov tshuaj hormones protein uas feem ntau yog tsim los ntawm cov islet cells hauv cov ntaub so ntswg pancreatic. Nws koom nrog hauv lub cev cov piam thaj metabolism thiab tswj lub cev cov ntshav qab zib kom sib npaug. Ntshav qab zib INS qib tuaj yeem cuam tshuam txog kev kho mob ntshav qab zib. Nws tuaj yeem pom los ntawm Table 5 uas piv nrog cov pab pawg ib txwm muaj, qib INS ntawm cov nas dub hauv pawg qauv tau txo qis (P<0.01). After 4 weeks of intervention in the administration group, compared with the model group, the fasting serum insulin levels of black mice in each administration group increased to varying degrees, among which the total glycosides of Cistanche deserticola and Tartscholaris had the most obvious increase. The results showed that each administration group could increase the serum insulin level of db/db black mice and promote the secretion of insulin by pancreatic β-cells.

3.2.3 Cov txiaj ntsig ntawm qhov sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola / Cistanche deserticola ntawm daim siab oxidative kev ntxhov siab ntawm db / db cov nas dub

Qib MDA nyob rau hauv daim siab ntawm cov nas dub nyob rau hauv cov qauv pab pawg neeg yog ho siab tshaj qhov uas nyob rau hauv ib txwm pab pawg neeg (P<0.05). After 4 weeks of intervention in the medication group, compared with the model group, the MDA levels of each medication group decreased to varying degrees (P < 0.05), among which Cistanche deserticola total polysaccharides, Cistanche deserticola total oligosaccharides, Cistanche deserticola total glycosides, and wine The level of MDA in the liver tissue of black mice treated with total glycosides of Cistanche deserticola and total oligosaccharides of Cistanche deserticola showed the most obvious decrease (P<0.05 or P<0.01). The results showed that Cistanche deserticola had a certain effect on reducing the MDA level in the liver tissue of db/db black mice, and the improvement effect was better than that of the alcoholic acid deserticola administration group.

Kev ua haujlwm SOD hauv daim siab ntawm cov nas dub hauv pawg qauv yog qis dua li hauv pawg tswj hwm ib txwm muaj (P<0.05). When different extract parts of Cistanche deserticola and Tartscholaris are administered orally, the SOD activity in the liver tissue of db / db black mice can be increased. Compared with the model group, each administration group can restore SOD activity, among which the total glycosides of Cistanche deserticola group are different. There is statistical significance (P<0.05). The results are shown in Table 6.

3.2.4 Cov txiaj ntsig ntawm qhov sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola / Cistanche deserticola ntawm cov rog rog metabolism ntawm db / db cov nas dub

Txhawm rau ntsuas cov txiaj ntsig ntawm Cistanche deserticola / Tartrol deserticola ntawm cov ntshav lipid metabolism theem ntawm db / db cov nas dub, cov cim ntsig txog ntshav qab zib lipid metabolism tau tshawb pom hauv qhov kev sim. Nws tuaj yeem pom los ntawm Table 7 uas piv nrog cov pab pawg tswj hwm ib txwm muaj, qib ntshav LDL-C ntawm pawg qauv tau nce ntxiv (P<0.05), while the HDL-C level was decreased. After 4 weeks of drug intervention, compared with the model group, the serum TG, TC and LDL-C levels of the Cistanche deserticola total polysaccharide, total oligosaccharide and total glycosides group and the Tartistan deserticola total polysaccharide, total oligosaccharide and total glycosides group were significantly lower than those of the model group. The levels of HDL-C decreased to varying degrees and the levels of HDL-C increased, among which the Cistanche deserticola total glycosides group had the effect of improving TC levels (P<0.05). The results showed that Cistanche deserticola and tartaric acid deserticola could improve the lipid metabolism disorder of db / db black mice to a certain extent.

3.2.5 Qhov cuam tshuam ntawm qhov sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola / Cistanche deserticola ntawm UA, Cr thiab mALB hauv ntshav thiab zis ntawm db / db cov nas dub

Tom qab 4 lub lis piam ntawm kev tswj hwm, piv nrog cov qauv pab pawg, UA, Cr thiab mALB qib ntawm Cistanche deserticola tag nrho cov polysaccharides, tag nrho cov oligosaccharides thiab tag nrho cov cawv glycosides pawg ntawm Cistanche deserticola raug txo kom sib txawv. Raws li kev txo qis Cr, UA thiab mALB hauv cov zis (P＜0). Cr thiab mALB hauv zis (P＜0.01). 05 lub p<0.01), ethanol can improve the UA level in plasma (P<0.05), and the total glycosides of Cistanche cantxo plasma UA, Cr thiab mALB qib ntawm db/db nas dub rau qee yam.

3.2.6 Qhov cuam tshuam ntawm cov khoom lag luam sib txawv thiab cov khoom rho tawm ntawm Cistanche deserticola ntawm histopathology ntawm pancreas thiab raum ntawm db/db cov nas dub

Tsis muaj kev puas tsuaj rau cov kab mob pancreatic ntawm cov nas dub hauv pawg tswj hwm ib txwm muaj. Cov npoo ntshiab ntawm cov islets tau pom. Cov islet hlwb tau oval thiab ua kom zoo nyob rau hauv cytoplasm. Cov nuclei yog puag ncig thiab cov islets muaj ntau dua thiab loj dua. Cov kab mob pancreatic ntawm cov nas dub hauv pawg qauv tsis muaj cov islets tiav. Cov npoo ntawm cov islets tsis meej thiab cov duab tsis tiav. Cov hlwb ntawm cov islets tau teeb tsa hauv qhov tsis sib xws, nrog tus lej me, atrophy, thiab kev puas tsuaj loj rau cov txiav. Piv nrog rau cov nas hauv pawg qauv, tom qab 4 lub lis piam ntawm kev tswj hwm intragastric ntawm qhov sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola / Tartarchus deserticola, pancreatic islet cov ntaub so ntswg ntawm pawg tswj hwm ntawm txhua qhov chaw muab rho tawm ib nrab, cov islet cell ntim tau nce mus rau qhov sib txawv, thiab cov islet cells muaj ciam teb meej. , qhov kev tsis sib haum xeeb yog txhim kho. Ntawm lawv, tag nrho cov glycosides pawg ntawm Cistanche deserticola thiab Tartschalne deserticola pom qhov zoo tshaj plaws rov qab.

ib txwm tswj pawg

Cistanche deserticola polysaccharide pawg

Tartschalna campestris polysaccharide pawg

pawg qauv

Cistanche deserticola oligosaccharide pawg

Tartracean oligosaccharide pawg

pawg tswj qhov zoo

Cistanche deserticola tag nrho glycosides pawg

Tag nrho glycosides pawg ntawm Tarthola cistanche

Daim duab 3 Cov teebmeem ntawm qhov sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola / Cistanche deserticola ntawm pancreatic pathology ntawm db/db cov nas dub (H&E, 200 ×)

Tsis muaj kev puas tsuaj rau lub raum ntawm cov nas dub hauv pawg tswj hwm ib txwm muaj. Cov qauv glomerular, cov duab thiab qhov piv rau glomerular cyst kab noj hniav yog qhov qub. Cov phab ntsa ntawm cov tubules proximal thiab distal yog nyias thiab tuab, thiab cov lumen loj yog qhov qub. Cov qauv, cov duab, thiab qhov loj ntawm lub glomeruli ntawm cov nas dub hauv pawg qauv yog qhov tsis sib xws, thiab glomeruli atrophied thiab ob lub raum.

Lub zais pa kab noj hniav ploj mus, cov tubules nyob ze thiab qhov nruab nrab muaj qhov tsis sib xws ntawm phab ntsa tuab, qhov sib txawv lumen ntau thiab tsawg, thaj tsam tsis meej, o thiab necrosis, thiab ntau ntawm cov cell infiltration. Piv nrog rau cov nas hauv pawg qauv, tom qab 4 lub lis piam ntawm kev tswj hwm intragastric ntawm cov khoom sib txawv ntawm Cistanche deserticola / Tamarococcus deserticola, lub raum cov ntaub so ntswg ntawm pawg tswj hwm ntawm txhua qhov muab rho tawm tau txhim kho, thiab cov qauv glomerular, cov duab, thiab qhov loj me tau nyob ze. mus rau qhov qub, thiab cov tubules ze ze Lub phab ntsa ntawm lub distal tubule yog nyias, qhov loj ntawm lub lumen tau txhim kho, qhov o yuav txo qis, thiab qhov me me ntawm cov kab mob ntawm cov cell infiltration tseem tuaj yeem pom hauv qee qhov chaw. Ntawm lawv, pawg Cistanche deserticola polysaccharide thiab pawg neeg haus dej cawv deserticola polysaccharide tau zoo tshaj plaws.

3.2.7 Qhov kev qhia ntawm hom-IV collagen nyob rau hauv lub raum cov ntaub so ntswg ntawm db / db dub nas los ntawm txawv extracted qhov chaw ntawm Cistanche deserticola / Litose deserticola zoo ib yam li cov pab pawg neeg ib txwm.

Daim duab 4. Cov teebmeem ntawm qhov sib txawv ntawm Cistanche deserticola/Tamarococcus deserticola ntawm lub raum pathology ntawm db/db cov nas dub (H&E, 200 ×). Qhov kev qhia ntawm hom-IV collagen protein nyob rau hauv ob lub raum ntawm cov nas dub nyob rau hauv cov qauv pab pawg neeg hloov pauv loj (P＜ 0 .05). Piv nrog rau cov qauv pab pawg, qhov nruab nrab lub raum membrane matrix cheeb tsam ntawm cov nas dub nyob rau hauv txhua pawg tswj hwm tau nce. Ntawm lawv, kev qhia ntawm hom-IV collagen hauv pawg Cistanche deserticola polysaccharide, pawg tartschistanche deserticola oligosaccharide, thiab tartschistanche deserticola tag nrho glycosides pawg tau txhim kho, thaum lub raum tsis muaj qhov sib txawv tseem ceeb hauv kev qhia ntawm hom-IV collagen. hauv qab daim nyias nyias.

ib txwm tswj pawg

Cistanche deserticola polysaccharide pawg

Cistanche deserticola polysaccharide pawg

pawg qauv

Cistanche deserticola oligosaccharide pawg

Cistanche deserticola oligosaccharide pawg

pawg tswj qhov zoo

Cistanche deserticola tag nrho glycosides pawg

Cistanche deserticola tag nrho glycosides pawg

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