Cov teebmeem Ntawm Cov Extracted Txawv Ntawm Cistanche Deserticola Ntawm Hypoglycemic Los Ntawm Db / Db Dub nas

Abstract:

Lub Hom Phiaj:Txhawm rau tshawb nrhiav cov teebmeem thiab cov txheej txheem ntawm cov khoom sib txawv ntawm Cistanche deserticola thiab Tartschula deserticola ntawm kev txo cov ntshav qab zib hauv db / db cov nas dub.

Txoj kev:Covtag nrho polysaccharides,Cov tshuaj oligosaccharides,ua glycosides ntawm Cistanche deserticolathiab Tartschula deserticola tau enriched, thiab lawv cov ntsiab lus tau muab piv thiab soj ntsuam. db/db cov nas dub tau raug xaiv los ua cov tsiaj sim. Tom qab 28 hnub ntawm kev tswj hwm, lub cev qhov hnyav, kev noj zaub mov, thiab kev haus dej ntawm cov nas dub tau raug kaw, thiab kuaj pom cov ntshav qabzib. ELISA kuaj pomcov tshuaj insulin(INS), glycosylated hemoglobin (HbA1c), thiabdaim siab triglyceride. ester (TG), tag nrho cov cholesterol (TC),tsawg-density lipoprotein(LDL-C),high-density lipoprotein(HDL-C),malondialdehyde(MDA),superoxide dismutase(SOD), plasma, thiab zis Theem ntawm uric acid (UA),creatinine ua(CR), thiab zis micro protein (MALB) qib hauv cov kua; txoj kev HE staining yog npaj rau cov kab mob ntawm cov txiav thiab raum cov ntaub so ntswg; IHC ntsuas cov qib protein qhia ntawm lub raum HOM -IV Collagen.

Cov txiaj ntsig: Cov ntsiab lus ntawm polysaccharide ib feem ntawm Cistanche deserticola txo qis tom qab steaming nrog cawv. Cov ntsiab lus ntawm betaine nyob rau hauv oligosaccharide ib feem ntawm Cistanche deserticola nce tom qab steaming nrog cawu cub. Cov ntsiab lus ntawm tag nrho glycosides echinaceaside poob me ntsis tom qab steaming nrog cawv. Cov ntsiab lus ntawm verbascoside txo qis. Cov ntsiab lus ntawm heterogeneous nplooj Cimicifugarin cov ntsiab lus nce. Piv nrog rau cov qauv pab pawg, sib txawv ntawm qhov sib txawv ntawm Cistanche deserticola thiab Tartscholaris tuaj yeem txo lub cev qhov hnyav, yoo ntshav qabzib (FBG), qhov ncauj qabzib siab (OGTT), HbA1c, insulin resistance index (HOMA-IR), islet -cell secretion index ( HOMA-), MDA, LDL-C, UA, Cr thiab mALB, INS thiab HDL-C muaj kev nce siab (P<0.05, P<0.01), and can improve the pathological damage of pancreas and kidney and ty ｐｅＩＶ Expression of collagen.

Kev xaus Tag nrho cov glycosides ntawm Cistanche deserticola thiab Tartschalis deserticola muaj cov nyhuv hypoglycemic zoo dua, thiab cov polysaccharides thiab oligosaccharides ntawm Tartschalne deserticola kuj muaj qee yam kev cuam tshuam hypoglycemic.

Ntsiab lus:Cistanche deserticola;tag nrho polysaccharides; tag nrhoCov tshuaj oligosaccharides; tag nrho glycosides; db / db nas dub; hypoglycemia

Acteoside in Cistanche (2)

Organic CISTANCHE EXTRACT nrog 25% ECHINACOSIDE thiab 9% ACTEOSIDE

Cistanche deserticolayog lub qia qhuav nrog scaly nplooj ntawm Cistanche deserticola los yog Cistanche deserticola. Khawb cov yub nyob rau lub caij nplooj ntoo hlav thaum lawv nyuam qhuav unearthed los yog ua ntej lub freezing av nyob rau hauv lub caij nplooj zeeg, tshem tawm cov qia, txiav lawv mus rau hauv seem, thiab qhuav lawv nyob rau hauv lub hnub [1]. Cistanche deserticola muaj cov teebmeem ntawm nourishing raum yang, replenishing essence thiab ntshav, thiab moisturizing cov hnyuv thiab laxatives. Nws yog siv rau kev kho mob raum tsis txaus yang, tsis muaj peev xwm ntawm cov ntsiab lus thiab cov ntshav, impotence thiab infertility, mob thiab qaug zog ntawm lub duav thiab lub hauv caug, tsis muaj zog ntawm cov leeg thiab pob txha, plab hnyuv dryness, thiab cem quav. Cov tshuaj pharmacological ntawm Cistanche deserticola suav nrog kev tiv thaiv kev laus, tshuaj tua kab mob antioxidant, tiv thaiv dementia, tiv thaiv qaug zog, thiab laxative teebmeem [2-3]

. Lub ntsiab Cheebtsam ntawmCistanche deserticolasuav nrogphenylethanoid glycosides, iridoid glycosides, polysaccharides, thiab lwm yam, ntawm cov phenylethanoid glycosidesCawv glycosides, thiabpolysaccharidesyog cov tshuaj tseem ceeb [4].

Kev tshawb fawb tshuaj niaj hnub no qhia tau hais tias Cistanche deserticola polysaccharide muaj ntau yam tshuaj xws li tswj kev tiv thaiv kab mob, tiv thaiv kev laus, txhim kho kev kawm thiab kev nco muaj peev xwm, tiv thaiv cov hlab ntsha, tiv thaiv kab mob siab, tiv thaiv kab mob, tiv thaiv qog, cuam tshuam rau cov hnyuv [5] . Cistanche deserticola tag nrho glycosides muaj cov dej num ntawm kev txhawb nqa lub siab thiab lub raum, ua kom cov ntsiab lus thiab cov ntshav ntxiv, antioxidant, tiv thaiv kev laus, kev tiv thaiv kab mob, thiab kev tiv thaiv neuroprotection [6]. "Suav Pharmocopoeia" 2020 tsab

(Part 1) muaj ob hom kev ua tiav ntawm Cistanche deserticola ntsiav tshuaj thiab cawv deserticola. Kev tshawb fawb ua ntej los ntawm pab pawg tshawb fawb pom tias cov khoom nyoos ntawm Cistanche deserticola nyiam ua kom cov hnyuv moisturize thiab txo cov cem quav, thaum Liquor deserticola tau txhim kho lub raum-tonifying thiab yang-txhim kho cov teebmeem. Tag nrho cov glycosides thiab tag nrho cov polysaccharides ntawm Cistanche deserticola yog qhov zoo rau kev ntxiv rau lub raum yang, thiab oligosaccharides ntawm Cistanche deserticola yog qhov zoo rau moisturizing cov hnyuv thiab laxative [7]. Thaum lub sij hawm caw-steaming txheej txheem ntawm Cistanche deserticola, phenylethanoid glycoside Cheebtsam hloov pauv loj, cov ntsiab lus of verbascoside txo qis, thaum cov ntsiab lus ntawm nws cov isomer verbascoside nce ntau [8]. Hom 2 mob ntshav qab zib mellitus yog cov kab mob metabolic uas tshwm sim los ntawm insulin tsis kam, nrog rau cov qauv thiab kev ua haujlwm puas tsuaj rau pancreatic-cells, uas ua rau muaj cov tshuaj insulin tsis txaus thiab feem ntau tshwm sim los ntawm cov ntshav qab zib siab [9]. Cov tshuaj suav tshuaj suav hais tias ntshav qab zib feem ntau cuam tshuam rau lub raum essence, uas khaws cov essence. Kev tsis txaus ntawm cov ntsiab lus tuaj yeem ua rau mob ntshav qab zib thiab ua rau mob ntshav qab zib nephropathy. Cov tsos mob ntawmmob ntshav qab zib hom 2feem ntau suav nrog kev tso zis ntau dhau, qaug zog, nqhis dej thiab nqhis dej, thiab tshaib plab tom qab noj ntau dhau, uas zoo ib yam li cov raum yang tsis muaj peev xwm. Yog li ntawd, kev kho mob ntshav qab zib mellitus hauv cov tshuaj suav tshuaj yog tsom rau tonifying lub raum thiab ntxiv rau lub raum yang [10-11].

Raws li cov lus ceeb toom hauv cov ntaub ntawv, phenylethanol glycosides hauv Cistanche deserticola, xws li echinaceaside thiab verbascoside, tuaj yeem tsis tsuas yog inhibit qhov nce hauv cov ntshav qab zib tom qab tab sis kuj txhim kho cov piam thaj kam rau ntawm cov hmoov txhuv nplej siab cov nas [12-15]. Qhov no kuj qhia tau tias Cistanche deserticola muaj cov txiaj ntsig ntawm kev txo cov ntshav qab zib.

db / db Cov nas dub yog congenitally rog rog T2DM nas tshwm sim los ntawm qhov tsis xws luag ntawm leptin receptor gene. Leptin receptor noob poob haujlwm thiab hyperinsulinemia tshwm sim hauv 2 lub lis piam tom qab yug me nyuam. 3 ~ Kev rog rog tshwm sim hauv 4 lub lis piam, thiab hyperglycemia hnyav heev tshwm sim hauv 8 lub lis piam, nrog rau cov tshuaj insulin tsis kam thiab -cell tsis ua haujlwm [16-17]. Kev tuag feem ntau tshwm sim li ntawm 8 mus rau 10 lub hlis thiab tej zaum yuav nyuaj los ntawm lub raum tsis ua haujlwm. Kab mob 18］

. Hauv txoj kev tshawb no, db / db cov nas dub tau siv los ua cov kev sim ua kom muaj txiaj ntsig ntawm tag nrho cov polysaccharides, tag nrho cov oligosaccharides, thiab tag nrho glycosides ntawm Cistanche deserticola thiab Tartschalis deserticola feem. Lawv tau ua qhov kev tshawb fawb sib piv ntawm cov teebmeem hypoglycemic ntawm cov khoom sib txawv. Nws yuav tsum tau muab ib tug scientific hauv paus rau kev ua mechanism ntawm Cistanche deserticola wine steaming.

Acteoside in Cistanche (13)

1 Cov khoom siv thiab cov cuab yeej siv

1.1 Kev sim tsiaj txhu BKS ntawm spontaneous type 2 diabetes nas qauv. Cg-Dock7m + / + Leprdb / JNju (genotype: db / db diabetes

Kab mob dub nas) Txiv neej 7-lub lim tiam-hnub dub nas, lub cev hnyav 35 ~ 40 g, DB / db m+ (genotype: db / m + nas dub) Txiv neej 7- lub lis piam-laus nas dub, lub cev hnyav 18 ~ 20 g, los ntawm muab los ntawm Nanjing University-Nanjing lub koom haum ntawm Biomedicine thiab Model Tsiaj lub koom haum ntawm Nanjing University. Cov phiaj xwm kev tshawb fawb no tau tshuaj xyuas los ntawm Pawg Neeg Saib Xyuas Tsiaj Ncaj Ncees ntawm Liaoning University of Traditional Chinese Medicine, thiab tus lej pom zoo yog 2018YSDW-030-02.

1.2 Cov tshuaj thiab cov tshuaj reagents

Cov khoom siv tshuaj Cistanche deserticola tau sau los ntawm Alxa, Inner Mongolia (batch tooj: 20190508-1) thaum lub Tsib Hlis 2019. Nws tau txheeb xyuas tias Cistanche suab puam (Cistanche suab puam) ntawm Orobanchiaceae cog los ntawm xibfwb Zhai Yanjun ntawm Tsev Kawm Ntawv Pharmacy, Liaoning University of Traditional suav tshuaj. Qhuav scaly nplooj ntawm cola YC Ma) Fleshy qia, cov qauv khaws cia nyob rau hauv Liaoning Provincial Traditional suav tshuaj Processing Technology Industry Innovation Center.

Echinacoside siv tshuaj (batch naj npawb: YUAV TSUM -

13080801, purity Ntau dua lossis sib npaug li 98%), verbascoside (verbascoside) cov khoom siv (batch tooj: YUAV TSUM-13122711, purity Ntau dua lossis sib npaug li 98%), isomulascoside Glycoside (isoacteoside) cov khoom siv (batch tooj: YUAV TSUM-15081001, purity Ntau dua lossis sib npaug li 99.77%), betaine siv cov khoom siv (batch tooj: 894-200001, purity Ntau dua lossis sib npaug li 98%) tau yuav los ntawm Tuam Tshoj Lub Tsev Haujlwm Saib Xyuas Kev Tswj Xyuas Tshuaj thiab Cov khoom siv roj ntsha; D101 macroporous cob yog muab los ntawm Chengdu Manster Biotechnology Co., Ltd.

Nas anti-8-hydroxydeoxyguanosine (8-}OHdG) monoclonal antibody [batch tooj: ab62623, Abcam (Shanghai) Trading Co., Ltd.]; PAS (batch tooj: G1281, Beijing Solebao Technology Co., Ltd.); Anti-fluorescence Quenching mounting tov (Hai Biyuntian Biotechnology Co., Ltd.); SP9001 Rabbit SP nrhiav cov khoom siv (Wuxi Aorui Dongyuan Biotechnology Co., Ltd.); DAB, insulin (INS), glycosylated hemoglobin (HbA1c), triglyceride (TG), nas tag nrho cov cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), uric acid (UA) creatinine (Cr), malondialdehyde (MDA), superoxide Tshuaj dismutase (SOD), urinary microalbumin

Dawb (mALB) cov khoom siv (Shanghai Langton Biotechnology Co., Ltd.). Cov roj cholesterol, xylene, tsis muaj ethanol, acetonitrile, thiab methanol yog cov qib chromatographic, dej yog ultrapure dej, thiab lwm yam reagents yog ntawm analytical qib.

ORGANIC CISTANCHE liqour

1.3 Cov cuab yeej tseem ceeb

ACQUITY H-Class UPLC (Lub Tuam Txhab Dej Hauv Tebchaws Meskas); Cov ntsuas ntshav qabzib thiab cov ntsuas ntshav qabzib (Bayer Healthcare Co., Ltd.); AE240 hom 1/100, 000 hluav taws xob analytical tshuav nyiaj li cas (Mettler, Switzerland) - Toledo Company); FA1004 hluav taws xob tshuav nyiaj li cas (Shanghai Precision Scientific Instrument Co., Ltd.); BN-518 Tsis siv neeg cov ntaub so ntswg embedding tshuab (Hubei Bernard Medical Technology Co., Ltd.); Hluav taws xob qhov kub kom qhuav qhov cub (Zhongwei Electronic Instrument Co., Ltd.); Leica RM2245 microtome (Leica Biosys, Lub teb chaws Yelemees) cov khoom); pH acidity meter (Shanghai Pengshun Scientific Instrument Co., Ltd.); Milli-Q Integral 3 hom dej purifier (Fabkis Molsheim tuam txhab).

ORGANIC CISTANCHE liqour