Cov teebmeem ntawm Cistanche Ntawm Kev Tiv Thaiv Kev Laus Thiab Kev Tiv Thaiv Kev Ua Haujlwm Ntawm Cov Qauv Kev Laus Ua Ntej Thiab Tom Qab Ua Haujlwm

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

Abstract: Lub Hom Phiaj: Tshawb nrhiav cov khoom lag luam sib txawv ntawmCistanchesherba hauv D - galactose los tiv thaiv kev laus muaj nuj nqi hauv nas thiab cov teebmeem ntawm lub cev tiv thaiv kab mob. Txoj kev: Peb muab cov khoom sib txawv ntawm Cistanches herba. SD nas tau muab faib ua pawg tswj hwm dawb paug, pab pawg neeg laus laus, pab pawg vitamin E, cistanche siab, nruab nrab, thiab qis pawg pawg, cawv cistanche siab, nruab nrab, thiab qis pawg. Nrog D - galactose laus nas qauv tau tsim, tom qab kev kho mob, peb tau kuaj pom cov ntsiab lus ntawm MDA, SOD, thiab TSIS MUAJ nyob rau hauv cov ntshav ntawm txhua pab nas thiab suav cov poov xab index thiab thymus index; thiab tom qab ntawd pom cov kev hloov pauv morphological ntawm cov nqaij mos thiab mtDNA hauv lub hlwb. Cov txiaj ntsig: MDA thiab NO hauv cov ntshav ntawm cov laus qauv nas nce ntxiv (P<0. 01)="" and="" sod="" decreased="" significantly="" (fvo.="" 01).="" the="" model="" group="" had="" a="" tendency="" to="" decrease,="" thymus="" and="" spleen="" coefficient="" had="" obvious="" pathological="" spleen="" injury,="" and="" mtdna="" in="" the="" brain="" was="" missing.="" different="" processed="" products="" of="">Cistanchesherba tuaj yeem txo qhov MDA thiab NO (P<0. 01="" or="" p=""><0. 05).="" sod="" had="" a="" rising="" trend,="" improving="" the="" spleen="" index="" and="" thymus="" index,="" improving="" the="" pathological="" injury="" of="" the="" spleen.="" the="" mtdna="" in="" the="" brain="" of="" the="" high="" dose="" group="" and="" positive="" medicine="" group="" did="" not="" miss.="" and="" processing="" products="" were="" better="" than="" the="" raw="" cistanches="" herba.="" the="" high="" dose="" group="" was="" better="" than="" the="" low="" dose="" group.="" conclusion:="" different="" processing="" products="" of="" cistanches="" herbs="" have="" different="" anti-aging="" effects="" and="" immune="">

Ntsiab lus: Cistanches herba; kev ua; kev laus; tiv thaiv kab mob

Cov tshuaj suav tshuajCistanche deserticolaYC Ma orCistanche deserticolaYC Ma los yog C. tubulosa (Schenk) Wight yog qhov qhuav, scaly fleshy qia ntawmCistanche deserticola(Schenk) Yog. Nws yog sov nyob rau hauv qhov, qab zib thiab qab ntsev nyob rau hauv saj, thiab nyob rau hauv lub raum thiab txoj hnyuv loj meridian. Nws yog ib qho tseem ceeb rau ntxiv dag zog rau lub raum thiab ntxiv dag zog rau yang Tshuaj hu ua "desert ginseng" (2). Cistanche tau luam tawm thawj zaug hauv "Shen Nong's Materia Medica". Nws yog teev raws li qib saum toj kawg nkaus. Nws muaj cov txiaj ntsig ntawm kev sau cov ntsiab lus thiab nourishing marrow, nourishing lub raum thiab ntxiv dag zog rau yang, pleasing xim thiab lub neej ntev, nourishing ntshav thiab moisturizing dryness, etc. . Cov mob xws li mob, mob, mob ntshav, thiab lwm yam! 3_5]o Lub xyoo 2010 ntawm "Lub Pharmacopoeia ntawm Cov Neeg Koom pheej ntawm Tuam Tshoj" muajCistanche tubulosathiabCistanche E.

Raws li lub cev muaj hnub nyoog, yuav muaj qee qhov degradation hauv ob qho tib si physiological functions thiab morphological qauv. Qhov no yog qhov peb feem ntau hu ua "aging". Nws manifestations yog feem ntau deterioration ntawm lub nruab nrog cev, cov ntaub so ntswg, thiab hlwb, xws li cov ntaub so ntswg elasticity, atrophy ntawm lub cev, thiab txo tus naj npawb ntawm cov cell, uas yuav ua rau lub cev muaj ntau yam dej num yuav maj mam poob. Kev sim tau lees paub tiasCistanchemuaj kev tiv thaiv kev laus "Kuv", tab sis cov kev tshawb fawb sib piv ntawm cov tshuaj tiv thaiv kev laus ntawmCistancheua ntej thiab tom qab ua tiav tseem tsis tau tshaj tawm. Qhov kev sim no tsuas yog kawm txog qhovlos tiv thaiv kev lausntawmCistancheua ntej thiab tom qab ua tiav thiab muab lub hauv paus kev tshawb fawb los kawm txog cov txheej txheem ntawm kev laus.

Echinacoside

1 Cov khoom siv sim

1.1 Kev sim tsiaj

SD nas, hnyav 180-220 g. SPF yog muab los ntawm Liaoning Changsheng Biotechnology Co., Ltd., daim ntawv tso cai naj npawb: SCXK (Liao) 2015-0001.

1.2 Tshuaj thiab tshuaj reagents

Cistanchetau muas los ntawm Alashan qus nqaij tshauv nyob rau hauv Inner Mongolia, thiab tau txheeb xyuas raws li lub qhuav fleshy qia nrog nplai nplooj ntawmCistanche deserticolaYC Ma los ntawm xibfwb Zhai Yanjun ntawm peb lub tsev kho mob; Txoj kev npaj ntawm txhua pab pawg ntawm cov tshuaj ntsuam xyuas yog raws li nram no, raw cistanche: raw Lub cistanche yog steamed rau 2 teev, txiav rau hauv 6 hli tuab slices, thiab qhuav ntawm 70P. Noj Cistanche slices thiab rho tawm 3 zaug hauv dej, tom qab ntawd muab cov decoction thiab mloog zoo rau qee qhov concentration. Kev npaj Cistanche: Noj Cistanche nyoos thiab soak hauv daj cawu cub rau 8 teev, ntxiv 1: 1, chav rau 16 teev, txiav rau hauv 6 hli tuab slices, thiab qhuav ntawm 70 T. Coj lub Cistanche slices thiab extract 3 zaug nrog dej, Tom qab ntawd muab cov decoction thiab concentrate nws mus rau ib qho concentration. Vitamin E soft capsules (batch tooj 1407061, Shenyang Hongqi Pharmaceutical Co., Ltd.); D-galactose (batch tooj 710B055, Beijing Soleibao Technology Co., Ltd.); Nitric oxide (NO) cov khoom siv (batch tooj: BPE30262); Malondialdehyde (batch tooj: BPE30262) MDA) cov khoom siv (batch tooj BPE30527); superoxide dismutase (SOD) cov khoom siv (batch naj npawb: BPE3O387); tag nrho yog muab los ntawm Shanghai Langdon Biotechnology Co., Ltd.; kab-hom tsiaj mitochondrial DNA extraction kit (yuav los ntawm Beijing Lab Technology Co., Ltd.); Cov khoom siv PCR (yuav los ntawm TIANGEN Reagent Company); Lwm cov reagents yog domestically ntshiab.

1.3 Cov khoom siv sim

Gene cycler (Hangzhou Longkey Scientific Instrument Co., Ltd.); Microplate nyeem ntawv (Thermo Fisher Technology Co., Ltd., USA); Qhov kub thiab av noo chamber (Shanghai Yiheng Scientific Instrument Co., Ltd.); TGL-16C hom qhia lub tshuab centrifuge benchtop (Shanghai Anting Scientific Instrument Factory); Vortex Tov Khoom (Shanghai Qingpu Huxi Instrument Factory); Electronic Balance (Shanghai Yousheng Weighing Apparatus Co., Ltd.); Vortex Tov Khoom (Shanghai Qingpu Huxi Instrument Factory).

2 Kev sim txoj kev

2.1 Tsiaj qauv thiab pab pawg

Tom qab 1 lub lis piam ntawm kev yoo mov, cov nas tau muab faib ua ib pawg dawb huv, pawg qauv, pawg tswj hwm,Cistanchepawg siab, nruab nrab, thiab qis koob, thiab B. striata: siab, nruab nrab, thiab qis koob, txhua pawg yog 9 pawg 8 daim. Tsuas yog cov pab pawg dawb huv, lwm pab pawg tau txhaj tshuaj D-galactose 167.5 mg / kg subcutaneously thaum sawv ntxov; cov pab pawg neeg dawb paug tau txhaj nrog qhov sib npaug ntawm cov kua qaub ib txwm; thaum tav su, qhov siab, nruab nrab thiab qis ntawm cov nqaij tshauv thiab cawv tau muab 5.48 thiab 2 raws li. 74, 1.37 g / kg ntawm cov khoom siv tshuaj ntsuam xyuas, cov pab pawg tswj tau zoo tau muab 2.75 mg / mL vitamin E, pab pawg neeg dawb huv thiab cov qauv pab pawg tau muab qhov sib npaug ntawm cov kua qaub ib txwm los ntawm kev tswj hwm intragastric. Rau 6 lub lis piam sib law liag, lawv thaum kawg raug muab tua thiab ntau yam ntsuas tau ntsuas.

2.2 Kev txiav txim siab ntawm SOD, MDA, thiab tsis muaj cov ntsiab lus hauv cov tshuaj nas

Cov ntshav tau sau los ntawm lub plab aorta ntawm nas, centrifuged ntawm 3000 r / min rau 15 feeb kom cais cov ntshav, thiab khaws cia ntawm -20 Y rau kev siv tom qab. MDA.SOD.NO kev txiav txim siab cov ntsiab lus yog ua raws li cov lus qhia ntawm cov khoom siv kuaj.

2.3 Qhov cuam tshuam rau qhov hnyav ntawm lub cev tiv thaiv kab mob hauv cov laus qauv nas

Cov nas nyob hauv txhua pab pawg tau hnyav thiab muab txi tom qab noj ntshav. Cov poov xab thiab thymus raug muab ntxuav nrog ntsev, qhuav, thiab hnyav, thiab cov poov xab thiab thymus coefficients raug xam.

2.4 Kev soj ntsuam ntawm spleen cov ntaub so ntswg morphology

Cov spleens hnyav tau kho nrog 4 feem pua ​​​​ntawm paraformaldehyde, embedded nyob rau hauv paraffin, sectioned, thiab stained nrog HE. Cov kev hloov pauv hauv cov kab mob pathological ntawm tus po nyob rau hauv txhua pab pawg tau pom nyob rau hauv lub tshuab tsom.

2.5 Cov txheej txheem txheeb cais

Siv SPSS software los ua ib txoj kev tsom xam ntawm qhov sib txawv thiab LSD-t-test rau kev sib piv ua ke.

2.6 Kev kuaj pom ntawm mtDNA kev hloov pauv hauv lub hlwb

Cov ntaub so ntswg nas tau muab tso rau hauv ib lub mortar nrog kua nitrogen, sai sai hauv av rau hauv cov hmoov, thiab tom qab ntawd xa mus rau cov ntaub ntawv txoj kev, raws li cov lus qhia rau cov ntaub so ntswg hlwb mtDNA extraction.

PCR amplification pom qhov tshem tawm ntawm mtDNA4834 fragment: cov primers xa mus rau qhov ua tiav ntawm nas mtDNA genome qhia los ntawm Gada-leta li al. (1898), thiab cov primers yog synthesized los ntawm Shanghai Shengang Industrial Co., Ltd. Pl: 5'-GCGAAGCTTAGAGCGTTTAAC-3'P2: 5'AGGAGATAAGGAAGCCTGC -3' P3: 5'-AG-GACTTAACCAGACCCAAAGACG{ {13}}, P4: 5'-CCTTTTTTCTGATAGGCGGGG-3,

PCR cov tshuaj tiv thaiv: DNA template 1 |xL, 2 x Taq PCR MasterMix 25 |xL, ddH2O 20 jiL, 2 primers txhua. Amplification tej yam kev mob: thawj 95 Y denaturation rau 5 min; 95 T denaturation rau 30 s, 56 T annealing rau 30 s, 72 T extension rau 45 s, tag nrho ntawm 35 cycles; qhov kawg 72 T txuas ntxiv rau 7 feeb. Cov khoom lag luam amplified tau raug rau 1.5 feem pua ​​agarose gel electrophoresis.

Acteoside

3 Cov txiaj ntsig thiab kev txheeb xyuas

3.1 Cov nyhuv ntawmCistanchentawm lub cev cov cim ntawm kev laus nas

Piv nrog rau cov pab pawg neeg dawb huv, cov nas laus pom cov tsho loj thiab cov xim daj, cov tawv nqaij xoob thiab npub, cov tawv nqaij xoob, cov plaub hau poob, tsis qab los noj mov, txo qis kev ua haujlwm, qeeb ntawm kev txav, txha nraub qaum, tsis muaj npe, qeeb teb thiab lwm yam kev laus tshwm sim. Lub sijhawm txhaj tshuaj ntev dua, qhov pom tseeb dua cov tsos mob. Piv nrog rau pawg neeg laus, cov nas lub siab lub ntsws, lub tsho tiv no xim, qab los noj mov, lub cev zoo, thiab cov tshuaj tiv thaiv rhiab tau zoo tuaj nrog kev nce sijhawm ntawm kev tswj hwm hauv txhua pab pawg tswj hwm.Cistanche. Ntawm lawv, tsis muaj qhov sib txawv ntawm cov khoom lag luam siab thiab pab pawg tswj hwm.

3.2 Cov nyhuv ntawmCistanchenyob rau hauv cov ntsiab lus ntawm MDA, SOD, thiab NO nyob rau hauv cov ntshav ntawm cov laus qauv nas

Cov txiaj ntsig tau pom nyob rau hauv Table I. Piv nrog rau pawg dawb huv, pawg qauv mda.no tau nce ntau ntxiv (P<0.01), and="" sod="" was="" significantly="" reduced="" (p=""><0.01); compared="" with="" the="" model="" group,="" each="" treatment="" group="" mda.no="" was="" significantly="" lower=""><0.01 or=""><0.05), sod="" has="" a="" rising="" trend,="" but="" it="" is="" not="" statistically="" significant;="" compared="" with="" the="" high-dose="" group="" of="">Cistanche, MDA.N0 ntawm pawg nruab nrab thiab qis qis ntawm Cistanche tau txo qis (F<0.05 ),="" sod="" tends="" to="" increase;="" compared="" with="" the="" high-dose="" group="" of="" cistanche,="" the="" mda.no="" of="" the="" middle="" and="" low-dose="" groups="" of="" cistanche="" rose="" significantly="" (p=""><0.01 or="" p=""><0.05), and="" the="" sod="" tended="" to="" decrease;="" compared="" with="" the="" jiu="" nvrong="" gao="" dose="" group,="" the="" mda.no="" of="" the="" high-dose="" eggplant="" paste="" group="" increased="" significantly="" (p=""><0.01), and="" sod="" tended="" to="" decrease.="" this="" result="" shows="" that="" the="" high-dose="" group="" is="" better="" than="" the="" low-dose="" group="" compared="" with="" different="" dose="" groups,="" and="" the="" treatment="" effect="" of="" the="" product="" group="" is="" better="" than="" that="" of="" the="" raw="" product="" group="" compared="" with="" the="" same="" dose="" group.="">

3.3 NwsCov txiaj ntsig ntawm Cistanchentawm lub cev tsis muaj zog ntawm kev laus qauv nas Chi

3.3.1 Cov txiaj ntsig ntawm Cistanche ntawm spleen Performance index thiab thymus Performance index ntawm cov laus qauv nas

Cov txiaj ntsig tau pom nyob rau hauv Table 2. Piv nrog rau pawg dawb paug, cov coefficient ntawm thymus thiab spleen hauv pawg qauv yog g

Muaj ib qho kev poob qis, tab sis nws tsis yog qhov tseem ceeb hauv kev txheeb cais. Piv nrog rau pawg qauv, thymus thiab spleen coefficients ntawm txhua pab pawg kho mob tau nce ntau (F<0.05); compared="" with="" the="" high-dose="" cistanche="">

Kev sib piv pab pawg, thymus thiab spleen coefficients ntawm nruab nrab thiab qis pawg ntawmCistanchemuaj lub siab xav txo

Muaj peev xwm; piv nrog cov koob tshuaj siabCistanche deserticolapab pawg siab, thymus, thiab spleen ntawm nruab nrab thiab qis-dose wine ntxhais paste pawg

Lub visceral coefficient muaj ib qho kev poob qis; piv nrog cov koob tshuaj siabCistanche deserticolahigh-dose pawg, thymus thiab spleen coefficients ntawm high-dosecistanche deserticolapab pawg neeg noj tshuaj muaj qhov txo qis. Qhov kev tshwm sim no qhia tau hais tias cov pab pawg neeg koob tshuaj siab zoo dua li cov pab pawg tsawg dua piv nrog cov pab pawg sib txawv, thiab cov txiaj ntsig kev kho mob ntawm pawg cov khoom lag luam zoo dua li cov khoom siv raw khoom piv nrog tib pawg tshuaj.

Table 1 Sib piv ntawm SOD.MDA.NO cov ntsiab lus hauv cov ntshav ntawm cov nas hauv txhua pab pawg (n. =8,x ±s)

Nco tseg:. Piv nrog rau kuv tus qauv pab pawg, P<005.><001; j="" air-day="" group="" comparison.=""><[)01; tlilll="" high-dose="" ether="" group="" jqi="" [i_low-dose="" group="" comparison.=""><005.><(). ()1;="" the="" high-dose="" product="" group="" is="" compared="" with="" the="" product="" tl="" and="" ff="" [journal="" f="" group.="" })=""><0.05.1l><(1o1; product="" high="" yanlian="" group="" q.="" pindaoli="" {group="" comparison.=""><005.><>

3.3.2 Cov txiaj ntsig ntawm kev hloov pauv pathological hauv tus poCistanchelaus qauv nas. Cov pab pawg neeg dawb paug muaj ib qho nyias nyias thiab tsis sib xws spleen capsule, ntshiab splenic trabeculae tsis o, ntshiab kev sib cais ntawm liab thiab dawb pulp, ntau tus po spleen nodules, thiab tib qhov loj, nyob rau hauv nruab nrab ntawm tus po, tus naj npawb ntawm cov hlwb nyob rau hauv lub lymphatic sheath nyob ib ncig ntawm. arterioles yog tuab. Nyob rau hauv cov qauv pab pawg neeg, tus po yog thickened, lub trabecula thiab cortex tau o, liab thiab dawb pulp yog tsis meej, lub dawb pulp tau txo qis, tus naj npawb ntawm lymphocytes raug txo, ib tug loj tus naj npawb ntawm inflammatory hlwb infiltrated, thiab lub morphological qauv raug puas. Txawm hais tias qhov luaj li cas ntawm tus po nodules nyob rau hauv txhua pab pawg neeg kho mob txawv, cov dawb thiab liab pulp ciam teb tau meej. Tus naj npawb ntawm cov hlwb hauv cov qog ntshav nyob ib ncig ntawm cov hlab ntsha me me ntawm tus po yog ntom, thiab cov morphology thiab cov qauv tau rov qab los. Tsis tas li ntawd, cov tshuaj tua kab mob siab heev tuaj yeem txhim kho cov kev hloov pauv pathological. Nws yog zoo dua li cov vitamin E pab pawg, nyob rau hauv uas lubCistanchecov khoom lag luam zoo dua li cov khoom siv raw cov khoom, thiab cov pab pawg neeg siab yog zoo dua li cov pab pawg tsawg. Saib cov ntawv ntxig X-XI.1-10 yog mtDNA nyob rau hauv lub hlwb ntawm nas nyob rau hauv lub dawb paug pab pawg neeg, pab pawg neeg qauv, pab pawg tswj, thiab elevated pab pawg neeg. mtDNA Daim duab 3 Electrophoresis tshwm sim ntawm mtDNA PCR amplification khoom nyob rau hauv nas hlwb (primer PI.P2)

3.4 Kev tshem tawm thiab hloov pauv ntawm mtDNA hauv lub hlwb

Raws li primers P3 thiab P4, ib qho kev txuag mtDNA feem ntawm txog 770bp tuaj yeem tau. Los ntawm daim duab 2 peb tuaj yeem pom tias cov phiaj xwm tawg tau nthuav dav hauv txhua txoj kab 9, qhia tias tag nrho 9 pawg ntawm mtDNA tau ua tiav lawm.

Determination of mtDNA4834 bp deletion mutation: If there is a rat mtDNA4834 bp deletion, primers P1 and P2 will amplify the 4834 bp loss and break the fusion gene fragment with a length of 597 bp. As shown in Figure 3, there is a clear DNA band between 500 l>p thiab 750 bp nyob rau hauv txoj kab 2, 4, 5, 7, 9, thiab 10. Cov fragments tau zoo tu qab tso thiab xa mus rau Hangzhou Baocheng Biotechnology Company rau sequencing. Muab piv nrog cov kab ke qhia los ntawm Gadateta li al. (1989), nws tau ua pov thawj tias muaj kev tshem tawm ntawm mtDNA fragments txog 4000-5000 bp. Yog li, nws qhia tau hais tias muaj txog 4834 bp tshem tawm ntawm mtDNA hauv lub hlwb ntawm pawg qauv. Txoj kab 1, 3, thiab 8 tsis muaj qhov tawg, qhia tias tsis muaj mitochondrial fragment deletions nyob rau hauv 1, 3, thiab 8. Cov txiaj ntsig tau pom tias tsis muaj cov seem uas ploj lawm hauv cov tshuaj tua kab mob siab ntau, pawg tshuaj zoo, thiab pab pawg neeg dawb huv, qhia tias cov tshuaj tua kab mob siab ntau ntawm cov poov xab tuaj yeem muaj kev tiv thaiv kev laus. Saib daim duab 2 ~ Daim duab 3.

1-10 yog mtDNA nyob rau hauv lub hlwb ntawm nas nyob rau hauv lub dawb paug pab pawg neeg, pab pawg neeg qauv, pab pawg tswj, thiab elevated pab pawg neeg. mtDNA Daim duab 3 Electrophoresis tshwm sim ntawm mtDNA PCR amplification khoom nyob rau hauv nas hlwb (primer PI.P2)

Cistanche: anti-aging

4 Kev sib tham

Cov qauv kev laus tshwm sim los ntawm kev tswj hwm intragastric ntawm D-galactose tam sim no yog cov qauv siv ntau dua. Cov qauv no tsuas yog txo cov kev ua ntawm ntau yam antioxidant enzymes, yog li ntawd muaj coob tus dawb radicals generated thaum lub sij hawm metabolism hauv lub cev, uas ua rau cov laus ntawm lub cev. Cov kev hloov hauv cov ntaub so ntswg morphology zoo ib yam li cov kev laus ntawm ntuj ⑻. Ntau qhov kev sim tau pom tias D-galactose-induced abnormal nce nyob rau hauv NO cov ntsiab lus nyob rau hauv lub hlwb thiab cerebellar cortex thiab ntshav ntawm cov laus nas. Cov txiaj ntsig ntawm txoj kev tshawb fawb no tau lees paub tias cov ntshav cov ntsiab lus ntawm cov nas laus tau nce siab dua li ntawm pawg ib txwm muaj, uas zoo ib yam li cov txiaj ntsig tau tshaj tawm hauv cov ntaub ntawv "-m.

Hauv qhov kev sim no, cov nyhuv tiv thaiv kev laus ntawm cov qauv kev laus ntawm cov nas ua ntej thiab tom qab kev ua haujlwm ntawmCistanchetau kawm. Cov txiaj ntsig tau pom cov ntshav MDA thiab N nyob rau hauv cov laus qauv nas. Nce nce (P<0.01), sod="" significantly="" decreased="" (p=""><0.01), the="" model="" group="" thymus="" and="" spleen="" coefficients="" have="" a="" tendency="" to="" decrease,="" the="" spleen="" has="" obvious="" pathological="" damage,="" and="" mtdna="" in="" the="" brain="" is="" also="" missing.="" different="" processed="" meat="" ash="" can="" significantly="" reduce="" the="" levels="" of="" mda="" and="" no="" (/=""><0.01 or=""><0.05), sod="" has="" a="" rising="" trend,="" increase="" spleen="" index="" and="" thymus="" index,="" improve="" spleen="" pathological="" damage,="" and="" high="" mtdna="" in="" the="" brain="" the="" dose="" group="" and="" the="" positive="" drug="" group="" also="" did="" not="" appear="" to="" be="" missing.="" and="" the="" prepared="" meat="" puree="" is="" better="" than="" the="" raw="" meat="" puree,="" and="" the="" high-dose="" group="" is="" better="" than="" the="" low-dose="" group.="" research="" in="" recent="" years="" believes="" that="" aging="" is="" also="" related="" to="" the="" lipid="" peroxidation="" of="" biological="" membranes="" caused="" by="" free="" radicals.="" sod="" "'-e="" is="" an="" enzyme="" that="" scavenges="" superoxide="" free="" radicals.="" the="" older="" the="" body,="" the="" weaker="" the="" body's="" sod="" activity="" and="" the="" fewer="" antioxidants.="" this="" will="" increase="" the="" free="" radical="" damage="" factor="" mda,,4~,7],="" and="" eventually="" accelerate="" aging.="" in="" addition,="" the="" results="" of="" the="" thymus="" and="" spleen="" coefficients="" of="" aging="" model="" rats="" indicate="" that="" roujing="" rong="" can="" delay="" aging="" by="" improving="" the="" immune="" function="" of="" the="" body.="" the="" results="" of="" the="" study="" hope="" to="" provide="" a="" scientific="" basis="" for="" the="" clinical="" application="" of="">Cistanche.