Cov teebmeem ntawm Cistanche Glycosides Ntawm Kev Kawm Thiab Kev Muaj Peev Xwm Nco

1. Niaj hnub nimno tshawb fawb Center rau tsoos suav tshuaj, Health Science Center, Peking University, Beijing 100083, Tuam Tshoj;

2. Department of Pharmacology, Tuam Tshoj Pharmaceutical University, Nanjing 210009, Suav

Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791

Abstract:Lub Hom Phiaj: Saib xyuas cov teebmeem ntawmcistanche glycosidesntawm kev kawm thiab kev nco ntawm nas nrog raum yang deficiency induced los ntawm hydrocortisone.Txoj kev:cistanche glycosidestau muab tshuaj sib law liag rau 30 hnub, thiab hydrocortisone tau muab rau hnub tim 11 thiab 18 (tshwj tsis yog kev tswj hwm) kom simulate lub raum yang deficiency qauv. Cov xwm txheej dav dav ntawm cov nas thiab lawv qhov kev tuag tau raug kaw. Kev ntsuas qib qis tau siv los ntsuas qhov kev kawm thiab kev nco muaj peev xwm ntawm nas. Cov txiaj ntsig: 1 cistanche glycosides txo cov tsos mob xws li kev ua haujlwm tsawg dua, piloerection, noj zaub mov tsawg thiab haus dej haus, tshee tshee los ntawm hydrocortisone hauv cov nas, thiab cov neeg tuag tau poob zoo li .2 cistanche glycosides ua rau cov nas latent lub sij hawm ntawm kev nce qib thiab txo qis. ua yuam kev lub sij hawm nyob rau hauv 5min.Conclusions: cistanche glycosides yuav txhim khu kev kawm thiab nco ntawm lub raum yang deficiency nas induced los ntawm hydrocortisone, thiab kuj yuav txo tau tus nqi ntawm cov nas tuag.

cistanche cuam tshuam rau Kev Kawm Thiab Kev Muaj Peev Xwm Memory

Ntsiab lus: cistanche, cistanche deserticola, cistanche tubolosa, cistanche glycosides; cistanche cov txiaj ntsig, raum Yang Deficiency; Kev kawm thiab kev nco,cistanche phiv, cistanche tubolosa cov txiaj ntsig, cistanche deserticola cov txiaj ntsig, cistanche extract, phenylethanoid glycosides

Kev laus ntawm cov pej xeem tau ua kom pom cov tshuaj muaj kev nyab xeeb thiab siv tau zoo rau kev tiv thaiv thiab kev kho mob Alzheimer's qhov chaw kub hauv kev tshawb fawb tshuaj. Nyob rau hauv xyoo tas los no, cov tshuaj suav tshuaj tau ua tiav zoo hauv kev kho mob Alzheimer's tus kab mob nrog rau txoj kev ua kom lub raum thiab pob txha thiab lub plawv thiab tus po. Cov tshuaj suav tshuajcistanchethawj zaug luam tawm hauv "Shen Nong's Materia Medica". Nws muaj cov teebmeem ntawm nourishing raum yang, nourishing essence thiab ntshav, thiab moisturizing cov hnyuv. Nws yog tam sim no dav siv nyob rau hauv kev kho mob ntawmsenile dementia, impotence, ib txwm cem quav, thiab lwm yam kab mob [1, 2]. Cov kev tshawb fawb tau pom tias nws muaj kev cuam tshuam los ntawm kev tiv thaiv kev dementia, tiv thaiv oxidation, tiv thaiv hypoxia, tiv thaiv hluav taws xob, thiab kev tiv thaiv kev ua haujlwm. Lub ntsiab tivthaiv yogphenylethanoid glycosides.

Covtag nrho glycosides ntawm cistancheyog muab rho tawm thiab refined los ntawm covqhuav fleshy stems ntawm Cistanche tubulosa(Schenk) Wight thiab muaj 90.7 phenethyl cawv glycosides, ntawm cov khoom tseem ceeb yogechinacoside cov tshuaj(echinacoside) 44.3. Kab lus no tau kawm txog qhov cuam tshuam ntawm tag nrhoglycosides ntawm cistanchentawm kev kawm thiab kev nco tsis zoo nyob rau hauv nas nrog raum-yang deficiency tshwm sim los ntawm hydrocortisone thiab muab lub hauv paus rau nws txoj kev loj hlob raws li ib tug nootropic thiab tshuaj tshiab rau covkev tiv thaiv thiab kev kho mob ntawm Alzheimer's disease.

1. Cov ntaub ntawv thiab cov txheej txheem

1.1 Tsiaj

120 noj qab nyob zoo Kunming nas, hnyav 18g rau 22g, yog ib nrab txiv neej thiab ib nrab, muab los ntawm Tsiaj Center ntawm Tuam Tshoj Pharmaceutical University. Daim ntawv pov thawj naj npawb yog Su Dong (Quality) 97004.

1.2 Tshuaj thiab tshuaj reagents

Cistanche, cistanche glycosides, extracts ntawm Cistanche tubulosa, muaj phenethyl cawv glycosides90.7, echinacoside cov tshuaj (echinacoside cov tshuaj) 44.3, tsaus xim av hmoov, muab los ntawm Chemistry Laboratory ntawm Niaj hnub nimno tshawb fawb Center ntawm tsoos suav tshuaj, Peking University, batch tooj 980601. Jinkui Shenqi Pills yog tsim los ntawm Henan Wanxi Pharmaceutical Factory, batch tooj yog 980607. Hydrocortisone txhaj yog tsim los ntawm Yangzhou Pharmaceutical Factory, tus lej batch yog 980301. Cov tshuaj hais saum toj no tau npaj nrog cov dej distilled los ua kom cov concentration xav tau rau kev sim ua ntej siv.

1.3 Apparatus

WX-Z nas platform yog tsim los ntawm lub koom haum ntawm Materia Medica, Suav Academy ntawm Kev Kho Mob Sciences.

1.4 Txoj kev

1.4.1 Pab pawg thiab kev tswj hwm Cov nas tau muab faib ua pawg tswj hwm ib txwm muaj (ua luv ua pab pawg ib txwm), pawg tswj hwm tus qauv (ua luv raws li pawg qauv), thiab pawg tswj hwm zoo (ua luv ua pab pawg zoo, Jinkuishenqi Pill 1.5g kg) raws li lawv lub cev hnyav. Thiab cov koob tshuaj ntau (piv txwv li, 400 mg kg), pawg koob tshuaj nruab nrab (200 mg kg), thiab pawg tshuaj qis (100 mg kg) ntawm tag nrho glycosides ntawmcistanche, 20 nyob rau hauv txhua pab pawg, ib nrab txiv neej thiab poj niam. Kev tswj hwm qhov ncauj, 0.2ml 10g lub cev qhov hnyav, pab pawg ib txwm, thiab cov qauv pab pawg tau muab qhov sib npaug ntawm cov dej distilled, ib hnub ib zaug, kev tswj hwm tas li rau 30 hnub. Txij hnub tim 11 ntawm qhov kev sim, tshwj tsis yog cov pab pawg ib txwm muaj, txhua pab pawg tau muab 1 mg hydrocortisone intragastrically rau 8 hnub sib law liag.

1.4.2 Kev soj ntsuam dav dav Thaum lub sij hawm sim, cov xwm txheej dav dav, thiab kev tuag ntawm txhua pawg nas raug soj ntsuam thiab sau tseg txhua hnub. Hnub 30) Xam cov nas tuag ntawm txhua pab pawg ntawm ob theem.

1.4.3 Platform dhia kev cob qhia thiab kev sim [7] Hnub 19 ntawm kev sim, cov nas tau muab tso rau hauv lub thawv tshuaj tiv thaiv ntawm lub platform kom hloov mus rau ib puag ncig rau 3 feeb, thiab tom qab ntawd energized nrog 36V alternating tam sim no. Tom qab cov nas xav tsis thoob, lawv feem ntau dhia mus rau lub platform Kom tsis txhob muaj hluav taws xob poob siab, cov nas dhia tawm ntawm lub platform thiab tau txais hluav taws xob poob siab thiab tom qab ntawd dhia rov qab mus rau lub platform thiab ua haujlwm rau 5 feeb. Kuaj tom qab 24 teev, muab tus nas tso rau ntawm lub platform, thiab sau lub sijhawm thaum tus nas dhia tawm ntawm lub platform thawj zaug (raws li lub sijhawm incubation) thiab cov lej yuam kev hauv 5 feeb. Qhov kev sim tau rov ua dua nyob rau hnub 30 ntawm qhov kev sim, thiab lub sijhawm incubation ntawm txhua tus tsiaj thiab tus lej yuam kev hauv 5 feeb raug kaw.

1.5 Kev ua lej

Cov txiaj ntsig ntawm lub sijhawm incubation thiab tus naj npawb ntawm qhov yuam kev hauv 5 min yog qhia raws li (x ± s), thiab cov ntaub ntawv tau ua tiav los ntawm t-test rau kev sib piv ntawm pab pawg. Rau cov tsiaj tuag, txoj kev yooj yim ncaj qha qhov tshwm sim yog siv rau kev ua lej.

2 Kev tshwm sim

2.1 Cov xwm txheej dav dav thiab kev txhim kho kev tuag ntawm hydrocortisone-induced yang-deficiency qauv nas.

Tom qab 8 hnub ntawm kev tswj hwm intragastric ntawm hydrocortisone, cov nas nyob rau hauv cov qauv pab pawg pom pom tseeb tsis tshua muaj zog, ntsug plaub hau, noj tsawg dua, haus dej tsawg, tshee, thiab lwm yam tsos mob ntawm yang deficiency, thiab qee cov tsiaj tuag, nrog kev tuag ntawm 42.1 feem pua. . Cov tsos mob saum toj no ntawm pab pawg zoo (Jinkuishenqi Pill 1500mg kg) thiab tag nrho glycosides ntawmcistanchetau txais txiaj ntsig zoo, thiab cov tsiaj tuag tau txo qis, uas yog qhov sib txawv ntawm pawg qauv, qhia tias tag nrho cov glycosides ntawmcistanchethiab cov tshuaj Jinkui Shenqi tuaj yeem txhim kho qhov kev mob ntawm cov nas uas muaj yang deficiency tshwm sim los ntawm hydrocortisone, thiab tuaj yeem txo qhov kev tuag ntawm cov nas.

2.1 Kev txhim kho cuam tshuam ntawm kev cuam tshuam

Cov qauv pab pawg tau muab hydrocortisone rau 8 hnub, piv nrog rau cov pab pawg ib txwm, lub sijhawm incubation ntawm lub platform kuaj tau luv luv, thiab cov lej yuam kev tau nce ntxiv, qhia tias cov qauv nas muaj kev kawm thiab kev nco tsis zoo. Tom qab 8 hnub ntawm kev tswj hwm hydrocortisone hauv pawg zoo thiab tag nrho glycosides ntawmcistanche, lub sij hawm incubation ntawm lub platform xeem tau ntev heev piv nrog rau cov qauv pab pawg neeg, thiab tus naj npawb ntawm kev ua yuam kev yog ho txo. Cortisone-induced kev kawm thiab kev nco tsis ua haujlwm hauv nas (Table 2). Tom qab 10 hnub ntawm kev tso tseg hydrocortisone nyob rau hauv pawg qauv, lub sij hawm incubation thiab tus naj npawb ntawm kev ua yuam kev nyob rau hauv lub platform dhia kev ntsuam xyuas tau zoo heev, uas yog tsis heev txawv los ntawm cov ib txwm pab pawg neeg. Tom qab nres hydrocortisone thiab txuas ntxiv kev tswj hwm rau 10 hnub hauv cov pab pawg zoo thiab tag nrho glycosides ntawm cistanche, latency ntawm lub platform kuaj thiab cov lej ntawm qhov yuam kev tseem tau txhim kho rau qee qhov piv nrog rau pawg qauv, tab sis tsis muaj qhov sib txawv ntawm cov lej. .

3 Kev sib tham

Kev kawm thiab kev nco yog qhov ua haujlwm siab heev ntawm lub hlwb thiab yog cov ntsiab lus uas tsim kev txawj ntse. Kev kawm thiab kev nco tsis zoo yog cov tsos mob sib koom ua ke los ntawm ntau yam kab mob encephalopathy, muaj qhov tshwm sim ntau, thiab ntau yam ua rau, xws li kev ua rau me nyuam yaus hyperactivity, tub ntxhais hluas chorea, cerebral lobe atrophy, neurasthenia, senile cerebrovascular sclerosis, thiab ntshav muab rau lub hlwb. Tsis muaj peev xwm, dementia, thiab lwm yam. Kev tshawb fawb txog kev kho mob niaj hnub no tau ua pov thawj tias kev kawm thiab kev nco tsis zoo cuam tshuam nrog cov kab mob neuronal synaptic kis tau tus mob hauv lub hlwb, nrog rau cov kab mob metabolic xws li neurotransmitters, hlwb, thiab lub zog. Cov tshuaj suav tshuaj suav hais tias muaj ob hom kev ua rau muaj kev puas siab puas ntsws, ntawm cov pov thawj pom tseeb rau cov neeg tsawg, thiab feem ntau yog cov mob tsis txaus, tshwj xeeb tshaj yog lub plawv, lub raum, thiab tus po tsis muaj peev xwm. Cov kev tshawb fawb soj ntsuam thiab kev sim tau pom tias ntau cov tshuaj suav tshuaj thiab tshuaj noj nrog cov tshuaj tonic tuaj yeem txhim kho kev kawm thiab kev nco tsis ua haujlwm rau qee yam [8]. Raws li physiological thiab biochemical hauv paus ntawm kev kawm thiab kev nco, tshuaj xws li M-choline receptor blockers, central nervous system inhibitors, hlwb protein synthesis inhibitors, hlwb hypoxia-ua rau cov neeg ua haujlwm feem ntau siv los ua ntau yam kev nco tsis zoo qauv [7]. Cerebral ischemia, hluav taws xob poob siab, kev ntxhov siab, thiab lwm yam tuaj yeem ua rau kev kawm thiab kev nco tsis zoo. Tsis tas li ntawd, TCM tau qhia ntau yam kev kawm thiab kev nco tsis zoo nyob rau hauv ntau yam tsiaj cov qauv ntawm "deficiency syndrome", xws li Qi deficiency, ntshav tsis muaj peev xwm, Yin deficiency, thiab Yang deficiency, tshwj xeeb tshaj yog tus po thiab lub raum deficiency qauv, uas yog ntau dua. haum rau kev kawm txog tshuaj tonic. [9]. Hauv qhov kev sim no, cov nas tau muab hydrocortisone rau 8 hnub ntxiv rau cov tsos mob ntawm yang deficiency, uas tseem pom tau tias kev kawm pom tseeb thiab kev nco tsis ua haujlwm, uas tau lees paub ntxiv tias yang-deficiency qauv nas tuaj yeem siv los ua qauv ntawm kev kawm thiab nco tsis ua haujlwm.

Cistancheyog ib txwm siv tshuaj ntxiv thiab tshuaj aphrodisiac. Nws muaj cov txiaj ntsig ntawm nourishing lub raum, sau cov essence, thiab moisturizing cov hnyuv. Nws yog hu ua cov tshuaj tseem ceeb rau nourishing lub essence thiab cov ntshav thiab nourishing lub raum. Nws tuaj yeem kho cov tsos mob xws li qaug zog, tsis nco qab, tsis hnov ​​​​lus, thiab enuresis tshwm sim los ntawm lub raum tsis txaus. Nws muaj txiaj ntsig zoo. Zhu Zhiming thiab lwm tus tau tshuaj xyuas cov tshuaj muaj pes tsawg leeg ntawm 46 cov ntawv sau tshuaj rau kev kho mob senile dementia luam tawm hauv cov ntawv xov xwm cuam tshuam los ntawm 1990 txog 1999 thiab pom tias Cistanche yog ib qho ntawm cov tshuaj nquag siv [1]. Cov kev sim tsiaj kuj tau lees paub tias Cistanche muaj kev tiv thaiv kev mob hlwb [10]. Hauv qhov kev sim no, tag nrho glycosides ntawm cistanche tuaj yeem txhim kho kev kawm thiab kev nco tsis ua haujlwm ntawm lub raum-yang deficiency qauv nas uas tshwm sim los ntawm hydrocortisone, uas qhia ntxiv tias cistanche muaj cov teebmeem nootropic, thiab qhia tias tag nrho glycosides ntawm cistanche yuav yog cov tsoos. Suav tshuaj cistanche rau nourishing lub raum. Lean active ingredient.

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