Cov txiaj ntsig ntawm Kev Kho Mob Nrog cysteine Rau Kev Cia Ntawm Cov Txiav Tshiab Cistanchesherba Ⅱ
Mar 27, 2024
2 Cov txiaj ntsig thiab kev txheeb xyuas
2.1 Cov nyhuv ntawm cysteine kev kho mob ntawm cov xim tshiab-txiav Cistanche deserticola.
Xim yog ib qho tseem ceeb tshaj plaws cov cim qhia txog kev laus thiab xim av ntawm cov tshiab-txiav Cistanche deserticola. Tus nqi L * sawv cev rau qhov ci ntawm cov txiv hmab txiv ntoo. Raws li pom hauv daim duab 1-A, 3 pawg
L * tus nqi ntawm kev txiav tshiabCistanche deserticolanyob rau hauv pawg cysteine-kho thiab pab pawg tswj tau pom qhov kev poob qis. Thiab thoob plaws lub sijhawm cia, L * tus nqi ntawm 1.5 g / L cysteine kev kho pab pawg tau nce siab dua li ntawm pawg tswj hwm (P<0.05), indicating that 1.5 g/L cysteine treatment can effectively alleviate the symptoms of fresh-cut Cistanche deserticola. Reduction in brightness. The a* value indicates the redness and greenness of the fruit. The greater the a* value, the redder the color, and vice versa. As shown in Figure 1-B, during the storage process, the a* value of fresh-cut Cistanche deserticolahauv txhua pab pawg tau nce ntxiv. Tus nqi a* ntawm pawg tswj hwm tau nce sai. Thaum kawg ntawm kev khaws cia, 1.5 g / L cysteine kev kho pab pawg yog 20.1%, 14.3%, thiab 11.9% qis dua li pawg tswj hwm, 0.5, thiab 1.0 g / L pab pawg kho cysteine, feem. . Raws li pom nyob rau hauv daim duab 1-C, thaum lub sij hawm tag nrho cov txheej txheem cia, b* qhov tseem ceeb ntawm peb pawg cysteine-kho thiab pawg tswj ntawm cov tshiab-txiav Cistanche deserticola pom ib qho kev nce zuj zus. Hnub tim 10, 1.5 g / L cysteine B * tus nqi ntawm cov amino acid kho pawg yog 11.8% qis dua li ntawm pawg tswj hwm (P<0.05). Description: Treating fresh-cut Cistanche deserticola with 1.5 g/L cysteine can delay the progression of a*, b* tus nqi nce, yog li txo qhov tob ntawm cov xim ntawm cov tshiab-txiav Cistanche deserticola.
Fig. 1 Cov nyhuv ntawm cysteine ntawm L * , a * , b * tus nqi tshiab ntawm Cistanchesherba
2.2 Cov txiaj ntsig ntawm kev kho cysteine rau TSS ntawm Cistanche deserticola tshiab
TSS tuaj yeem cuam tshuam cov cim tseem ceeb ntawm cov txiv hmab txiv ntoo kom loj hlob thiab khaws cia ruaj khov [21]. Raws li pom nyob rau hauv daim duab 2, TSS cov ntsiab lus nyob rau hauv txhua pab pawg pom ib tug downward trend, uas tej zaum yuav yog vim qhov txo nyob rau hauv soluble solids tshwm sim los ntawm cov metabolism ntawm tshiab-txiav Cistanche deserticola thaum lub sij hawm cia. Tom qab hnub thib 2 ntawm kev khaws cia, TSS cov ntsiab lus hauv 1.5 g / L cysteine kho pawg tau siab dua li hauv pawg tswj hwm (P<0.05). On the 10th day, the TSS content in the 1.5 g/L cysteine treatment group was 1.41 times that of the control group. . It shows that 1.5 g/L cysteine can effectively maintain the decrease in TSS content of fresh-cut Cistanche deserticola during storage.
2.3 Cov nyhuv ntawm cysteine kev kho mob ntawm soluble protein ntawm tshiab-txiav Cistanche deserticola
Soluble protein cov ntsiab lus tuaj yeem qhia meej meej txog ib qho ntawm lub cev qhia txog kev laus ntawm tes hauv cov txiv hmab txiv ntoo thiab zaub [22]. Raws li pom nyob rau hauv daim duab 3, cov ntsiab lus ntawm cov protein soluble ntawm txhua pab pawg tau pom ib qho kev poob qis hauv lub sijhawm tag nrho. Soluble protein cov ntsiab lus ntawm peb pawg cysteine-kho tau siab dua li ntawm pawg tswj. Qhov no yuav yog vim kev kho cysteine tuaj yeem ncua kev ua cov txiav tshiab. Lub hydrolysis ntawm Cistanche deserticola cov protein soluble yog txo, cia nws tswj cov khoom noj muaj txiaj ntsig zoo dua. Pab pawg tswj hwm thiab 0.5 thiab 1.0 g/L cysteine kho pawg muaj qhov tseem ceeb tshaj plaws nyob rau hnub 2 ntawm kev khaws cia, thaum 1.5 g/L cysteine kho pab pawg muaj tus nqi siab tshaj ntawm hnub 4.
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Tom qab hnub thib ob ntawm kev khaws cia, cov ntsiab lus ntawm cov protein soluble ntawm 1.5 g / L cysteine cov pab pawg kho mob tau nce siab dua li ntawm pawg tswj hwm (P<0.05), indicating that the 1.5 g/L cysteine treatment had a greater impact on the soluble protein content. The processing effect is relatively good.
2.4 Qhov cuam tshuam ntawm kev kho cysteine ntawm ASA thiab GSH cov ntsiab lus ntawm Cistanche deserticola tshiab
Ascorbic acid yog ib qho tseem ceeb hauv cov txiv hmab txiv ntoo thiab zaub. Raws li tau pom hauv daim duab {{0}}A, ASA cov ntsiab lus hauv Cistanche deserticola tshiab tau pom tias muaj kev poob qis dua piv rau hnub 0. Tom qab hnub 6, ASA cov ntsiab lus hauv pawg tswj hwm tau txo qis, thiab cov ASA cov ntsiab lus nyob rau hauv pab pawg neeg kho cysteine hloov siab dua li hauv pawg tswj hwm (P<0.05). On the 10th day, the ASA content in the 1.5 g/L cysteine treatment group was 2.67 times that of the control group (P<0.05). It follows that cysteine treatment can slow down the decrease in ASA content.
Cov ntsiab lus ntawm txo glutathione yog ze ze rau lub peev xwm antioxidant ntawm txiv hmab txiv ntoo thiab zaub. Raws li pom hauv daim duab {{0}}B, ob pawg tswj hwm thiab pab pawg kho mob tau pom qhov nce siab ua ntej thiab tom qab ntawd qhov kev poob qis. Cov pab pawg tswj hwm thiab 0.5 g/L cysteine kev kho pab pawg muaj qhov tseem ceeb tshaj plaws nyob rau hnub thib 2 ntawm kev khaws cia, thiab 1. hnub khaws cia. Hnub tim 10, GSH cov ntsiab lus ntawm 1.0 thiab 1.5 g / L cysteine kho pawg yog 47.3% thiab 47.6% siab dua li pawg tswj hwm (P<0.05). Description: Treatment with 1.0 and 1.5 g/L cysteine can effectively maintain the GSH content of fresh-cut Cistanche deserticola fruits during postharvest storage.
2.5 Cov nyhuv ntawm cysteine kev kho mob ntawm antioxidant enzyme kev ua ntawm tshiab-txiav Cistanche deserticola
Raws li tuaj yeem pom los ntawm daim duab {{0}}A, POD kev ua haujlwm ntawm peb pawg cysteine-kho thiab pab pawg tswj tau pom muaj kev nce ntxiv hauv 0 mus rau 6 hnub, mus txog qhov kawg ntawm Hnub 6, nrog kev kho mob 0.5, 1.0, thiab 1.5 g/L cysteine. pab pawg yog 1.39, 2.29, thiab 2.35 npaug ntawm pawg tswj hwm lub sijhawm no. Qhov no yuav yog vim qhov nce hauv cov pa oxygen hauv cov txiv hmab txiv ntoo los ntawm kev puas tsuaj los ntawm kev txiav tshiab [23]. Cov tshuaj antioxidant hauv cov ntaub so ntswg Cistanche deserticola tshiab tau tshwm sim. Nyob rau hauv lub xeev ntawm oxidative kev nyuaj siab, POD kev ua si nce. Tom qab hnub 6, POD kev ua haujlwm ntawm peb pawg cysteine-kho thiab pab pawg tswj tau pib txo qis. Hnub tim 2 mus rau 8th, POD kev ua ntawm 1.5 g / L cysteine kev kho pab pawg tau siab dua li pawg tswj hwm (P<0.05). On the 10th day of storage, the POD activity of the 1.5 g/L cysteine treatment group was higher than that of the control group. 39.8%. It is further known that cysteine treatment can increase POD activity. This may be due to mechanical damage causing the tissue to produce excessive H2O2, thereby inducing cysteine to increase POD activity and clearing excess H2O2 in a timely manner, which has a positive effect on delaying the aging of fresh-cut Cistanche deserticola. [twenty four].
Kev sib sau ntawm H2O2 ua rau oxidative puas tsuaj rau cov ntaub so ntswg, thiab CAT tuaj yeem ua rau cov decomposition ntawm H2O2 rau hauv H2O thiab O2, yog li txo cov oxidative puas los ntawm H2O2 [25]. Raws li pom nyob rau hauv daim duab 5-B, CAT kev ua ntawm tshiab-txiav Cistanche deserticola nyob rau hauv pab pawg tswj tau pom ib tug zuag qhia tag nrho downward trend thaum lub sij hawm cia. Qhov no tej zaum yuav yog vim li cas Cistanche deserticola cov ntaub so ntswg muaj hnub nyoog thaum lub sij hawm cia, ib tug loj npaum li cas ntawm reactive oxygen hom yog tsim nyob rau hauv cov ntaub so ntswg, yog li inhibiting lub CAT kev ua si [14]. 3 pawg ntawm cysteine kho cov pab pawg tau pom qhov sib txawv ntawm thawj zaug nce thiab tom qab ntawd txo qis, nrog rau qhov siab kawg rau hnub 4. Cov 0.5, 1.0, thiab 1.5 g/L cysteine kho pawg yog 95.3%, 118.8%, thiab 194.3% siab dua li pawg tswj hwm. , tom qab hnub 4, CAT kev ua ntawm peb pawg neeg kho cysteine pib txo qis. Thaum lub sijhawm tag nrho lub sijhawm cia, CAT kev ua haujlwm ntawm 1.5 g / L cysteine kev kho pab pawg tau siab dua li ntawm pawg tswj hwm. Hnub tim 10, CAT kev ua haujlwm ntawm 1.5 g / L cysteine cov pab pawg kho mob yog 4.13 npaug ntawm pawg tswj hwm (P<0.05). . It can be seen that cysteine treatment can induce and increase the CAT activity oftshiab-txiav Cistanche deserticolathiab txo cov oxidative puas tsuaj los ntawm H2O2.
Raws li pom nyob rau hauv daim duab {{0}}C, APX kev ua ntawm cov tshiab-txiav Cistanche deserticola tau qhia tag nrho cov qauv ntawm thawj zaug nce thiab tom qab ntawd txo qis thaum lub sij hawm cia, nrog rau qhov siab tshaj plaws nyob rau hnub 4. Thaum lub sij hawm tag nrho lub sij hawm cia, APX kev ua ntawm peb pawg cysteine-kho tau yeej ib txwm siab tshaj ntawm pawg tswj. Cov kev ua APX hauv 0.5, 1.0, thiab 1.5 g/L cysteine kho pawg yog 1.12, 1.23, thiab 1.29 npaug ntawm pawg tswj hwm. Tom qab hnub 4, APX kev ua haujlwm tau pib txo qis. Qhov kev ua APX hauv 1.5 g / L cysteine kho pab pawg yog txhua yam tseem ceeb. Siab tshaj pawg tswj hwm (P<0.05). On the 10th day, the APX activity of the 1.5 g/L cysteine treatment group was 2 times that of the control group.
Nws tuaj yeem pom tau tias kev kho mob cysteine tuaj yeem ua rau thiab nce APX kev ua haujlwm ntawm Cistanche deserticola tshiab. GR tuaj yeem txo oxidized glutathione (GSSG) rau GSH, uas tuaj yeem tshem tawm cov pa oxygen reactive, yog li tiv thaiv cov ntaub so ntswg los ntawm cov pa oxygen reactive [26]. Raws li pom hauv daim duab 5-D, GR cov haujlwm ntawm pawg tswj hwm tau txo qis rau hnub thib 2 ntawm kev khaws cia thiab nce siab rau hnub 4 ntawm kev cia. Peb pawg cysteine-kho tag nrho tau pom qhov sib txawv ntawm thawj zaug nce thiab tom qab ntawd txo qis. Thaum kawg ntawm kev khaws cia, muaj qhov sib txawv tseem ceeb ntawm 1.5 g / L cysteine kev kho pab pawg thiab pawg tswj hwm hnub 8 thiab 10 (P<0.05), which was 4.3 and 4.8 times higher than the control group respectively. After the 4th day of storage, the GR activity of the three cysteine-treated groups was higher than that of the control group, which shows that cysteine can maintain the decrease in GR activity. APX and GR are antioxidant enzymes that use ascorbic acid and oxidized glutathione as substrates respectively to remove excess H2O2 to protect cells from oxidative damage, protect cells from reactive oxygen species, and play an important role in the defense system [27 ].
2.6 Cov nyhuv ntawm cysteine kev kho mob ntawm PPO enzyme kev ua ntawm tshiab-txiav Cistanche deserticola
PPO yog ib qho ntawm cov enzymes tseem ceeb uas ua rau browning ntawm cov nqaij mos Cistanche deserticola tshiab. Raws li pom nyob rau hauv daim duab 6, thaum lub sij hawm tag nrho lub sij hawm cia, PPO kev ua ntawm pab pawg tswj thiab pab pawg kho mob pom ib tug downward trend piv nrog rau hnub 0, thiab pawg tswj thiab cov 0.5 thiab 1. Tus nqi siab tshaj plaws, thiab tsis muaj qhov sib txawv tseem ceeb ntawm PPO kev ua ub no tus nqi thaum lub sijhawm khaws cia piv nrog pawg tswj hwm (P﹥0.05). Tej zaum vim tias cov cysteine concentration tsawg, cov nyhuv inhibitory ntawm PPO kev ua haujlwm tsis pom tseeb. Cov pab pawg kho mob cysteine 1.5 g / L muaj qhov tseem ceeb tshaj plaws nyob rau hnub 4 thiab 6. Tsuas yog hnub 8, PPO kev ua haujlwm qis dua li ntawm pawg tswj hwm (P<0.05). At the end of storage, the PPO activity was lower than that of the control group. Low in control group
61.9%. Nws qhia tau hais tias cov cysteine daws concentration ntawm 1.5 g / L muaj ib tug tseem ceeb inhibitory nyhuv ntawm PPO kev ua ntawm tshiab-txiav Cistanche deserticola, thiab kuj muaj ib tug zoo ntxim rau ncua lub browning ntawm tshiab-txiav Cistanche deserticola.
2.7 Cov nyhuv ntawm kev kho cysteine rau tag nrho cov ntsiab lus phenolic thiab PAL kev ua ntawm cov tshiab-txiav Cistanche deserticola
Phenolic tshuaj yog dav faib nyob rau hauv txiv hmab txiv ntoo thiab zaub. Lawv tsis yog ib qho tseem ceeb antioxidant theem nrab metabolites ntawm txiv hmab txiv ntoo thiab zaub lawv tus kheej, tab sis kuj muaj txiaj ntsig zoo rau tib neeg kev noj qab haus huv [28]. Nws tuaj yeem pom los ntawm daim duab 7-A tias tag nrho cov ntsiab lus phenolic ntawm 3 cysteine-kho pab pawg thiab pawg tswj hwm tau txo qis rau hnub thib ob ntawm kev cia, thiab tom qab ntawd nce siab rau hnub 6 hauv pawg tswj hwm, thaum lub 3 pab pawg kho cysteine tau nce siab rau hnub 6. Tus nqi siab tshaj plaws tau tshwm sim rau hnub 4, thiab tag nrho cov ntsiab lus phenolic tau siab dua li ntawm pawg tswj hwm. Tej zaum nws yuav yog vim kev kho cysteine tuaj yeem ua rau muaj kev sib txuas thiab sib sau ntawm cov tshuaj phenolic hauv cov txiav Cistanche deserticola tshiab. Hnub tim 10 ntawm kev khaws cia, tag nrho cov ntsiab lus phenolic ntawm 1.5 g / L cysteine kev kho pab pawg tau nce siab dua li ntawm pawg tswj hwm (P<0.05), which was 1.96 times that of the control group. It can be seen that cysteine treatment can also delay the development of fresh-cut Cistanche deserticola Tag nrho cov ntsiab lus phenolic ntawm Chengdu poob.
PAL koom nrog hauv biosynthesis ntawm ntau yam metabolites theem nrab, xws li phenolics, thiab yog ib qho ntawm cov enzymes tseem ceeb tswj txoj hauv kev phenylpropanoid hauv cov txiv hmab txiv ntoo thiab zaub [29]. Tom qab cov txiv hmab txiv ntoo thiab zaub raug txiav tshiab, kev puas tsuaj yuav ua rau cov phenylpropanoid metabolism, induce lub synthesis ntawm phenolic tshuaj, thiab indirect ua rau browning [30]. Raws li pom nyob rau hauv daim duab 7-B, PAL kev ua ntawm peb pawg cysteine-kho thiab pawg tswj tau pom ib qho kev nce zuj zus. Tom qab 4 hnub ntawm kev khaws cia, qhov nce ntawm PAL kev ua haujlwm hauv 1.5 g / L cysteine-kho pab pawg tau nrawm dua, thiab pab pawg tswj tau pom qhov kev nce zuj zus. PAL kev ua haujlwm ntawm txhua pab pawg tau qis dua li ntawm pawg cysteine-kho, tej zaum vim tias kev kho cysteine tuaj yeem ua rau muaj kev nce hauv PAL kev ua si thiab ua kom cov synthesis thiab tsub zuj zuj ntawm cov tshuaj phenolic.
2.8 Qhov cuam tshuam ntawm kev kho cysteine ntawm H2O2 thiab MDA cov ntsiab lus ntawm Cistanche deserticola tshiab
Raws li pom nyob rau hauv daim duab 8-A, thaum lub sij hawm cia, H2O2 cov ntsiab lus ntawm cov tshiab-txiav Cistanche deserticola qhia ib tug tag nrho cov qauv ntawm thawj zaug nce thiab ces txo. Cov ntsiab lus H2O2 ntawm pawg tswj hwm yeej ib txwm siab dua li ntawm 3 pawg cysteine-kho. Ua ntej hnub 8, H2O2 cov ntsiab lus hauv 1.5 g / L cysteine therapy pab pawg tau qis dua li hauv pawg tswj hwm (P<0.05). On the 10th day, the H2O2 content in the 1.5 g/L cysteine treatment group was 29.7 lower than the control group. % (P＜0.05). During the entire storage period, the H2O2 content in the cysteine-treated group was lower than that in the control group. It can be seen that cysteine treatment can reduce the accumulation of hydrogen peroxide during the storage of fresh-cut Cistanche deserticola.
MDA content can be used to reflect the degree of cell membrane lipid peroxidation and can also indirectly measure the degree of cell damage [31]. It can be seen from Figure 8-B that the MDA content of the three cysteine-treated groups and the control group showed a significant upward trend, indicating that as the storage time increases, the cell membrane lipid peroxidation of fresh-cut Cistanche deserticola increases. On the second day of storage, the MDA content results of the 0.5 and 1.0 g/L cysteine treatment groups and the control group were not significant (P>{{0}}. hauv pawg tswj hwm. Hauv 10 hnub ntawm kev khaws cia, 0.5, 1.0, thiab 1.5 g / L cysteine cov pab pawg kho mob yog 29.2%, 48.7%, thiab 63.9% qis dua li pawg tswj hwm, feem. Thoob plaws lub sij hawm cia, pab pawg neeg kho cysteine
Cov ntsiab lus MDA tau qis dua li ntawm pawg tswj hwm. Kev kho Cysteine tuaj yeem ua rau qeeb qeeb ntawm MDA hauv cov txiav Cistanche deserticola tshiab, txo cov qib ntawm lipid peroxidation ntawm nws cov cell membrane, zoo dua tswj cov kev ncaj ncees ntawm cov cell membrane, thiab ncua kev laus thiab deterioration ntawm tshiab-txiav Cistanche deserticola.
Daim duab 8 Cov nyhuv ntawm cysteine rau H2 O2 thiab MDA cov ntsiab lus ntawm Cistanchesherba tshiab
3 Kev sib tham
Xim, soluble protein, soluble solids, thiab ascorbic acid yog cov cim tseem ceeb uas cuam tshuam rau kev khaws cia zoo ntawm txiv hmab txiv ntoo thiab zaub [32]. Kev tshawb fawb tau pom tias kev kho mob cysteine ntawm cov tshiab-txiav Cistanche deserticola tuaj yeem ncua qhov txo qis hauv L * tus nqi (brightness) thiab qhov nce hauv a * (redness) thiab b * (daj) qhov tseem ceeb, thiab qhov concentration ntawm 1.5 g / L cysteine cov nyhuv ua yog qhov zoo tshaj plaws. 1.5 g / L cysteine kuj tuaj yeem txo qis hauv cov protein uas muaj kuab lom, cov khoom soluble, thiab cov ntsiab lus ascorbic acid ntawm cov tshiab-txiav Cistanche deserticola thaum lub sij hawm cia, thiab muaj kev cuam tshuam zoo rau kev khaws cia zoo ntawm cov tshiab-txiav Cistanche deserticola.
Cov metabolism hauv cov pa oxygen nquag hauv cov txiv hmab txiv ntoo thiab zaub muaj feem cuam tshuam nrog kev khaws cia zoo thiab ntev ntawm cov txiv hmab txiv ntoo thiab zaub [33]. Vim muaj kev puas tsuaj rau cov khoom tshiab-txiav Cistanche deserticola, cov ntaub so ntswg raug puas tsuaj, uas yuav ua rau muaj kev cuam tshuam ntawm lub cev tsis zoo thiab biochemical, xws li tsub zuj zuj ntawm cov pa oxygen reactive, uas cuam tshuam ncaj qha rau kev khaws cia zoo [34]. Muaj ob hom ROS scavenging systems nyob rau hauv txiv hmab txiv ntoo thiab zaub hlwb: ib tug yog reactive oxygen hom scavenging enzymes, xws li POD, CAT, APX, GR, thiab lwm yam.; lwm qhov yog non-enzymatic scavenging systems, nrog rau cov tshuaj antioxidant xws li ASA thiab GSH [35]. Txoj kev tshawb no pom tias 1.5 g / L cysteine kev kho mob tuaj yeem txhim kho cov dej num ntawm POD, CAT, APX, thiab GR thaum lub sij hawm cia cov tshiab-txiav Cistanche deserticola, yog li txo cov tsub zuj zuj ntawm reactive oxygen hom thiab mitigating oxidative kev puas tsuaj ntawm tshiab-txiav. Cistanche deserticola.
Cov txiaj ntsig ntawm kev tshawb fawb ntawm Chen Chen [14] kuj tau pom tias kev kho cysteine tuaj yeem ua rau muaj kev cuam tshuam thiab ua rau cov haujlwm ntawm cov tshuaj tiv thaiv kab mob antioxidant xws li POD, CAT, APX, thiab GR thaum khaws cov txiv apples tshiab, thiab ncua kev laus thiab xim av ntawm cov tshiab. - txiav txiv apples. Cov txiaj ntsig tshawb fawb ntawm Feng Chengcheng [15] tau pom tias kev kho cysteine ntawm cov qos yaj ywm tshiab-txiav ntshav qab zib tuaj yeem inhibit POD enzyme kev ua haujlwm. Qhov sib txawv ntawm cov txiaj ntsig tuaj yeem cuam tshuam nrog hom txiv hmab txiv ntoo thiab zaub thiab kev ua haujlwm sib txawv. Tsis tas li ntawd, cov tshuaj tua kab mob endogenous ASA thiab GSH yuav tshem tawm cov khoom tsim ntawm ROS hauv cov ntaub so ntswg nyob rau hauv ib txwm muaj lub cev muaj zog thiab ua kom cov txiv hmab txiv ntoo zoo [36]. Hauv txoj kev tshawb no, 1.5 g / L cysteine kev kho mob tuaj yeem ncua qhov kev poob qis ntawm ASA thiab GSH cov ntsiab lus, tswj cov ntsiab lus antioxidant siab, txhim kho cov txiv hmab txiv ntoo reactive oxygen scavenging peev xwm, yog li txo oxidative kev puas tsuaj.
PPO tuaj yeem ua rau kev sib xyaw ua ke ntawm oxygen thiab polyphenols hauv cov txiv hmab txiv ntoo thiab zaub kom tsim quinones. Cov quinones polymerize los ua xim av sediments, ua rau browning ntawm txiv hmab txiv ntoo thiab zaub [19]. Kev kho mob tshiab-txiav Cistanche deserticola nrog 1.5 g / L cysteine tuaj yeem cuam tshuam PPO enzyme kev ua haujlwm, yog li ncua qhov tshwm sim ntawm browning. Lu Fan [18] thiab Wei Jie [11] pom tias kev tswj hwm huab cua tuaj yeem cuam tshuam PPO kev ua haujlwm ntawm Cistanche deserticola tshiab, ua kom xim av qeeb, thiab ua kom zoo dua khaws cia zoo ntawm Cistanche deserticola tshiab. Kev puas tsuaj rau cov tshuab tuaj yeem ua rau muaj kev nce hauv PAL kev ua haujlwm hauv cov txiv hmab txiv ntoo thiab zaub tshiab thiab ua kom cov khoom sib txuas ntawm phenolic [37]. Txoj kev tshawb no pom tias PAL enzyme kev ua thiab tag nrho cov ntsiab lus phenolic ntawm cov tshiab-txiav Cistanche deserticola tom qab kev kho mob cysteine ntau dua li cov pab pawg tswj, thiab 1.5 g / L cysteine kev kho mob concentration yog qhov zoo tshaj plaws, qhia tias cysteine tuaj yeem ua rau cov khoom tsim tawm. tshiab-txiav Cistanche deserticola. Mechanical puas induces cov nyhuv ntawm kev nce PAL kev ua ntawm Cistanche deserticola thiab accumulating tag nrho cov ntsiab lus phenolic.
4 Kev xaus
Txoj kev tshawb no qhia tau hais tias 1.5 g / L cysteine kev kho mob tuaj yeem txhim kho cov dej num ntawm POD, CAT, APX, thiab GR thaum lub sij hawm cia cov tshiab-txiav Cistanche deserticola, ncua kev txo qis hauv cov ntsiab lus ntawm cov protein soluble, TSS, ASA, thiab GSH. , thiab inhibit kev cuam tshuam ntawm MDA thiab H2O2. tsub zuj zuj, zoo dua khaws cia zoo ntawm tshiab-txiav Cistanche deserticola.
Cov ntaub ntawv
[1] Bi Cuicui, Liu Yinlu, Wei Fenfen, et al. Kev tshawb nrhiav kev nce qib ntawm cov tshuaj lom neeg tseem ceeb thiab cov dej num lom neeg ntawm Cistanche deserticola [J]. Kev Tshuaj Ntsuam Xyuas Tshuaj, 2019, 42(9): 1896-1900. BI CC, LIU YL, WEI FF, et al. Kev tshawb fawb kev nce qib ntawm cov ntsiab lus tshuaj lom neeg thiab cov dej num lom neeg ntawm Cistanche deserticola [J]. Tshuaj
Kev Ntsuam Xyuas Kev Tshawb Fawb, 2019, 42(9): 1896-1900.
[2] Feng Duo, Duan Hao, Lu Yanni, et al. Daim ntawv thov Cistanche deserticola hauv cov zaub mov noj qab haus huv hauv kuv lub tebchaws [J]. Food Science thiab Technology, 2021, 46(12): 76-81. FENG D, DUAN H, LYU YN, et al. Daim ntawv thov Cistanche deserticola Ma hauv cov khoom noj khoom haus hauv Suav teb [J]. Food Science thiab Technology, 2021, 46(12): 76-81.
[3] Wang Li, Cao Rui, Fang Yongyu, et al. Kev txiav txim siab ntawm cov ntsiab lus ntawm cov khoom xyaw nquag hauv Cistanche deserticola los ntawm UPLC-QQQMS method[J]. Suav Materia Medica, 2017, 40(2): 295-300. WANG LW, CAO R, FANG YY, et al. Kev txiav txim siab ntawm cov khoom siv tau zoo hauv Cistanche deserticola los ntawm UPLC-QQQMS[J]. Phau ntawv Journal of Chinese Medicinal Materials, 2017, 40(2): 295-300.
[4] DONG YH, GUO Q, LIU JJ, et al. Kev txiav txim siab ib txhij ntawm xya phenylethanoid glycosides hauv Cistanches Herba los ntawm ib qho cim uas siv cov kev suav tshiab ntawm cov txheeb ze kho qhov xwm txheej [J]. Phau ntawv Journal of Separation Science, 2018, 41(9) ): 1913 -1922.
[5] He Meng, You Lin, Bao Xiaowei, et al. Nyob rau hauv vitro antioxidant teebmeem ntawm Cistanche deserticola dej extract thiab nws cov nyhuv ntawm plab hnyuv muaj kab mob hauv nas [J]. Food Research and Development, 2020, 41(23): 44-50.
HE MM, YOU L, BAO XW, et al. Antioxidation ntawm Cistanche deserticola dej extract nyob rau hauv vitro thiab nws cov nyhuv ntawm txoj hnyuv muaj kab mob nyob rau hauv
mus [J]. Food Research and Development, 2020, 41(23): 44-50.
[6] Li Xu, Hu Jingping, Sun Xiaodong, et al. Kawm txog kev tiv thaiv kev qaug zog thiab cov txheej txheem ntawm Cistanche deserticola polysaccharide ntawm D-galactose-induced aging nas[J]. Hebei Tsob Ntoo Suav Tshuaj, 2019, 41(1): 96-100.
YAN L, HU JP, SUN XD, et al. Kawm txog kev tiv thaiv kev qaug zog thiab cov txheej txheem ntawm Cistanche deserticola polysaccharide ntawm D-galactoseinduced aging nas[J]. Hebei Journal of Traditional Chinese Medicine, 2019, 41(1): 96 -100.
[7] Ma Xiaoting, Zhang Shilei, Wang Zhiqiang, thiab lwm yam. Kawm txog cov nyhuv thiab cov txheej txheem ntawm Cistanche deserticola phenylethyl glycoside liposome ntawm hepatic stellate cell proliferation induced los ntawm rrPDGF-BB[J]. Hnyav
Qing Tshuaj, 2019, 48(10): 1630-1634. MA.
[8] Zhang B, Yang LL, DING SQ, et al. Anti-osteoporotic kev ua si ntawm cov tshuaj suav tshuaj suav tshuaj Cistanche deserticola ntawm pob txha
Metabolism ntawm ovariectomized nas los ntawm RANKL/RANK/TRAF6-txoj kev taw qhia kev sib kho[J]. Frontiers hauv Pharmacology, 2019, 10.
DOI: 10.3389/fpha.2019.01412.
[9] Liu Xinhong, Zheng Anmin. Kev tshawb fawb soj ntsuam ntawm 31 tus neeg mob cem quav kho nrog Cistanche deserticola decoction [J]. Tus Kws Kho Mob Hauv Zej Zog Suav, 2020, 36(4): 126-127. LIU XH, ZHENG A M. Kev soj ntsuam ntawm 31 tus neeg mob cem quav kho los ntawm Huangyuanyu Rou Cong Decoction[J]. Suav zej zog
Cov kws kho mob, 2020, 36(4): 126-127.
[10] Zhi Xu, Zhen YAqin, Tian Wei, et al. Kev tshawb fawb kev nce qib ntawm cov tshuaj lom neeg thiab cov tshuaj pharmacological ntawm Cistanche deserticola thiab kev kwv yees kev tsom xam ntawm cov cim zoo (Q-Marker) [J]. Suav tshuaj ntsuab, 2021, 52(9): 2758-2767. ZHI YJ, ZHEN YQ, TIAN W, et al. Kev tshawb nrhiav kev nce qib ntawm cov khoom siv tshuaj lom neeg thiab cov tshuaj pharmacological ntawm Cistanche deserticola thiab kev kwv yees tsom xam ntawm tus cim zoo (Q-Marker) [J]. Suav tshuaj ntsuab thiab tshuaj ntsuab, 2021, 52(9): 2758-2767.
