Cov nyhuv ntawm Ethanolic Extract ntawm cayratia Trifolia Ntawm Histologically raum nas qauv

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

Djoko Santoso: koj puas xav tau ntau tus thwjtim?^1*, Kuv Ketut Sudian², Anny Setijo Rahayu³thiab Muchamad Yunus⁴

¹Department of Internal Medicine, Kws Qhia Ntawv ntawm Tshuaj, Universitas Airlangga

^{2, 3}Department of Anatomic Pathology, Kws Qhia Ntawv ntawm Tshuaj, Universitas Airlangga

⁴Department of Veterinary Parasitology, Kws Qhia Ntawv ntawm Veterinary Medicine, Universitas Airlangga

*Cov neeg sau email: djoko-santoso@fk.unair.ac.id

Abstract.

Txoj kev tshawb no tau ua los txiav txim siab qhov tshwm sim ntawmCistanche extractntawm histological nasraumqauv. Tag nrho ntawm 12 tus neeg laus txiv neej Balb/c nas tau muab faib ua ob pawg, suav nrog rau nas nyob rau hauv txhua pab pawg. Thawj pab pawg ntawm 6 nas tau muab los ua pawg tswj hwm thiab tau txais 25 ml / kg BW qhov ncauj ntawm sodium carboxymethylcellulose rau 6 hnub. Hauv pab pawg thib ob, 6 nas tau muab 50 mg / kg BW ntawmCistancherho tawmnyob rau hauv sodium carboxymethylcellulose qhov ncauj rau 6 hnub. Nyob rau hauv txoj kev tshawb no, peb tau ua histologicalraumnas ntsuam xyuas tom qab 6 hnub ntawmCistancherho tawmkev tswj hwm. Cov nas histologicalraumNws tau raug suav hais tias yog tubular epithelium, glomerular, thiab focal mononuclear infiltrate. Cov nyhuv ntawm kev tswj hwm ntawmCistancherho tawmntawm histologicalraumntawm cov nas tshwm sim ib txwm tubular morphology nyob rau hauv cortical thiab medullary cheeb tsam ntawm lub raum hauv kev tswj hwmCistancherho tawmnas, tsis muajraumhloov histologically pom ib txwm architecture ntawm lub glomerulus thiab tubules, thaum nyob rau hauv tswj pab pawg nas, lub raum histologically thawj cim nrog mononuclear cell infiltration, tubular necrosis, glomerular congestion, thiab hyaline casts. Txoj kev tshawb fawb tau nthuav tawm cov kev hloov pauv histopathological txaus nyob rau hauv corticomedullary hlws ris ntawm nas lub raum, nrog rau tubular epithelial puas, tubular dilatation, thiab intratubular cast tsim.

Keywords: Balb/c nas,Cistancherho tawm, raum

Ethanolic Extract ntawmCistanchentawm Histologically raum

1. Taw qhia

Ob peb yam ntuj tsim muaj cov nroj tsuag tshuaj, ib qho ntawm cov nroj tsuag tshuaj yogCistanche. Ib tug ntau ntawm cov tebchaw muaj nyob rau hauvCistanchecov nroj tsuag muaj xws li bioactive compounds xws li daj wax roj, steroids, terpenoids, flavonoids, thiab tannins [1]. Phenols, flavonoids, thiab tannins tau txais kev saib xyuas vim tias lawv muaj cov txiaj ntsig zoo hauv kev tiv thaiv kab mob, txhawb kev noj qab haus huv, thiab cov tshuaj tiv thaiv kev laus raws li ntuj antioxidants [2]. Antioxidants tuaj yeem ua haujlwm los txo oxidative kev puas tsuaj los ntawm cov dawb radicals thiab reactive oxygen hom nyob rau hauv tej yam kev mob ntawm oxidative kev nyuaj siab nyob rau hauv tib neeg [3]. Ntau qhov chaw ntawmCistanchexws li stems, nplooj, thiab cov hauv paus hniav tau tshaj tawm tias muaj hydrocyanic acid thiab delphinidin. Nplooj ntawmCistanchetau tshaj tawm tias muaj ntau yam flavonoids xws li cyanide [1]. Kaempferol, myricetin, quercetin, triterpenes, thiab epifriedelanol muaj nyob rau saum huab cua ntawm cov nroj tsuag; cov noob thiab txiv hmab txiv ntoo muaj cyanogenic compounds; thiab nplooj kuj muaj stilbenes xws li piceid, resveratrol, vinifera, thiab ampelopsin [4].

Cov neeg mob ntshav qab zib mellitus txhawm rau txhawm rau kuaj ntshav qab zib ib txwm muaj tuaj yeem muab kev txhaj tshuaj ntawm cov noob nrog rau cov extract ntawm tubers ntawm qhov ncauj. Hauv ntau qhov xwm txheej xws li diuretics, qog nqaij hlav, neuralgia thiab plexopathy tuaj yeem siv tau los siv tag nrho cov nroj tsuag [5]. Lub xub ntiag ntawm alkaloids thiab flavonoids nyob rau hauvrho tawmntawmCistanchetuaj yeem siv rau kev ua haujlwm zoo dawb radicals scavenging. Antioxidants uas muaj cov extract ntawmCistanchenthuav tawm qhov zoo dawb radical scavenging kev ua si [6]. Ntau cov khoom ntawm cov nroj tsuag no tau tshaj tawm hauv cov kev tshawb fawb yav dhau los xws li tshuaj tua kab mob, tshuaj tua kab mob, tshuaj tua kab mob, tshuaj tua kab mob, tshuaj tiv thaiv kab mob, tshuaj tiv thaiv kab mob, tshuaj tua kab mob, tshuaj tiv thaiv kab mob, thiab diuretic zog. Txoj kev tshawb no tsom kom paub qhov cuam tshuam ncaj qha ntawm cov tshuaj tiv thaiv antioxidants thiab tiv thaiv dawb radical scavenging kev ua ntawm covCistancherho tawmlos ntawm inducing sodium carboxymethylcellulose uas tuaj yeem cuam tshuam histologicallyraumnta hauv tus qauv nas.

2. Cov ntaub ntawv thiab cov txheej txheem

Tag nrho ntawm 12 tus neeg laus txiv neej Balb/c nas tau muab faib ua ob pawg, suav nrog rau nas nyob rau hauv txhua pab pawg. Thawj pab pawg ntawm 6 nas tau muab los ua pawg tswj hwm thiab tau txais 25 ml / kg BW qhov ncauj ntawm sodium carboxymethylcellulose rau 6 hnub. Hauv pab pawg thib ob, 6 nas tau muab 50 mg / kg BW ntawm Cistancherho tawmnyob rau hauv sodium carboxymethylcellulose qhov ncauj rau 6 hnub. Nyob rau hauv txoj kev tshawb no, peb tau ua histologicalraumnas ntsuam xyuas tom qab 6 hnub ntawmCistancherho tawmkev tswj hwm. Lub histologicalraumCov nas tau suav hais tias yog tubular epithelium, glomerular, thiab focal mononuclear infiltrate.

3. Cov txiaj ntsig thiab kev sib tham

HauvCistancherho tawmpawg nas tswj hwm (Pawg 2), kuj zoo morphology ntawm tubular tshwm sim nyob rau hauv cortical thiab medullary cheeb tsam ntawmraumnyob rau hauv cov nas no, tsis muaj lub raum raug mob histologically nrog lub qub architecture ntawm glomerulus thiab tubules. Thaum nyob rau hauv sodium carboxymethylcellulose tswj cov nas pawg (tsis muajCistancheextract, Pawg 1) raum raug mob histologically thawj cim nrog mononuclear cell infiltration, tubular necrosis, glomerular congestion, thiab hyaline casts nyob rau hauv lub lumen ntawm tubular (Table 1 thiab daim duab 1).

Table 1. Cov qhab nias ntawm cov yam ntxwv histological ntawm nasraumcov ntaub so ntswg hauv cov nyhuv ntawmEthanolic Extract ntawmCistanchethiab sodium carboxymethylcellulose induced nephrotoxicity

Kev siv cov tshuaj tiv thaiv kab mob yog ib leeg thiab ntau yam ua rau muaj kev cuam tshuam, ib qho yog nephrotoxicity. Ntau cov lus teb ntawm kev siv tus neeg sawv cev yog ib qho tseem ceeb kom paub nrog rau qhov zaus ntawm kev tswj hwm, qhov ntau npaum li cas thiab cov koob tshuaj ntawm tus neeg sawv cev, cov lus teb ntawm cov tsiaj muaj qhov sib txawv ntawm qhov hnyav tuaj yeem tshwm sim mob (thaum ntxov) thiab ntev (advanced)raumraug mob [7]. Nws zoo nkaus li tias kev siv carboxymethylcellulose tuaj yeem ua rau muaj kev tsim cov cytokines pro-inflammatory, reactive oxygen hom, thiab proteolytic enzymes.

Cov kev tshawb fawb yav dhau los ntawm qhov mob ib puag ncig ua rau pom qhov mob tsis tu ncua nrog matrix degradation thiab lwm yam nrog cov sequelae [8]. Qee qhov xwm txheej nrog rau cov kev mob tshwm sim loj xws li kev pheej hmoo ntawmraumtsis ua hauj lwm, kev puas tsuaj rau plab, thiab lub sij hawm los ntshav ntev heev vim tsis muaj coagulation feem ntau tshwm sim los ntawm kev siv cov pro-inflammatory agents. [9]. Vim li no, cov tswv yim nonpharmacological thiab cov tshuaj pleev ib ce kom ua tiav qhov kev pom zoo kom txo qis kev txhawb nqa.

mob. Kev tswj hwm ntawm sodium carboxymethylcellulose ua rau muaj kev hloov pauv hauv histopathological ntawm corticomedullary hlws ris ntawm nas.lub raum, suav nrog tubular epithelial puas, tubular dilatation, thiab tsim cov casts hauv tubular lumen (Daim duab 1).

Nephropathy nyob rau hauv nas yog histologically characterized los ntawm degenerative kev hloov nyob rau hauv lub proximal tubule muaj xws li hydropic degeneration, pycnotic nuclei, nce cytoplasmic vesicles, cytoplasmic vacuolization, poob ntawm txhuam ciam teb, necrosis thiab apoptosis ntawm tubular hlwb, thiab desquamation ntawm necrotic cell ntawm tubular epithelium. tsim hyaline casts. [10]. Cov nyhuv ntawm kev siv sodium carboxymethylcellulose tshwm sim los ntawm kev nce oxidative kev nyuaj siab, tubulointerstitial o, thiab apoptosis. Cov txiaj ntsig ntawm txoj kev tshawb fawb tam sim no tau qhia tias sodium carboxymethylcellulose tuaj yeem tsim cov dawb radicals uas tuaj yeem ua rau tubular puas vim yog sodium carboxymethylcellulose intoxicated tsiaj, uas tau tshwm sim los ntawm kev nce ntawm oxidative kev nyuaj siab, mob, thiab apoptosis nyob rau hauv lub cev.lub raum[11]. Ib qho ntawm cov tswv yim siv ntau tshaj plaws yog saline hydration thiab yuam diuresis. Txawm hais tias saline hydration thiab yuam diuresis, nephrotoxicity yog nyob rau hauv thaj tsam ntawm 20-30 feem pua ​​[12].

4. Cov lus xaus

Cistancherho tawmsupplementation yuav txo tau sodium carboxymethyl cellulose-induced nephrotoxicity. Cov txiaj ntsig ntawm kev tshawb fawb pom tau tias muaj kev hloov pauv histopathological txaus nyob rau hauv corticomedullary junction ntawm nas.raum, nrog rau kev puas tsuaj rau tubular epithelium, tubular dilatation, thiab tsim ntawm intratubular cam khwb cia. Peb xav tias covCistancherho tawmmodulates oxidative kev nyuaj siab nyob rau hauv lub raum.

Kev lees paub

Txoj kev tshawb no tau nyiaj los ntawm Tus Thawj Coj ntawm Kev Kawm Qib Siab, Ministry of Education thiab Culture ntawm Republic of Indonesia, Excellence Research Grant of Higher Education rau daim ntawv cog lus tshawb fawb: 560/UN3.14/PT/2020.

Ethanolic Extract ntawmCistanchentawm Histologically raum

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