Cov nyhuv ntawm Cistanche Polysaccharides Rau Kev Loj Hlob Ntawm Lymphocytes

Xiangyan Wang, Yun Qi, Runlan Cai, Xiaohong Li, Meihua Yang, thiab Yue Shi

(Lub koom haum ntawm Tshuaj Kho Mob, Suav Academy ntawm Kev Kho Mob Sciences thiab Peking Union Medical College, Beijing 100193)

Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791

Abstract

Lub hom phiaj

Kawm txog cov teebmeem ntawmCistanchedeserticolapolysaccharide(CDPS) ntawm kev loj hlob ntawm lymphocytes hauv nas. Cov txheej txheem MTT tau siv los txheeb xyuas qhov kev loj hlob ntawm nas splenic lymphocytes. Cyclophosphamide (Cy) replicates lub immunocompromised tsiaj qauv thiab ntsuas tus po thiab thymus indexes ntawm ib txwm thiab immunocompromised tsiaj. Txoj kev thymocyte proliferation ntsuas qhov kev ua ntawm interleukin -2 (IL-2). Cov txiaj ntsig CDPS tau muaj qhov cuam tshuam loj heev ntawm mitogen (ConA thiab LPS) ua kom cov lymphocytes thiab cov hlwb tsis ua haujlwm thiab txhawb kev tso tawm ntawm lymphocyte IL-2. Intraperitoneal kev tswj hwm tau pom tias CDPS tuaj yeem ua rau muaj qhov nce siab ntawm tus po ntawm cov nas ib txwm muaj thiab cov kab mob tiv thaiv kab mob, thiab nws kuj muaj qhov cuam tshuam loj rau kev txo qis ntawm thymus index los ntawm Cy. Xaus CDPS tuaj yeem txhawb nqa kev loj hlob ntawm nas splenic lymphocytes, uas tej zaum yuav cuam tshuam nrog kev txhawb nqa ntawm IL-2 secretion.

Ntsiab lus:Cistanchepolysaccharide; Pleev index; thymus index; lymphocyte proliferation; interleukin -2

Cistanchepolysaccharidesmuaj ntau yam haujlwm

Nroj tsuagpolysaccharidestau txais kev saib xyuas thoob plaws vim lawv cov teebmeem universal immunomodulatory thiab lawv cov yam ntxwv tsis tshua muaj toxicity [1].Cistanchedeserticolayog ib tug qhuav fleshy qia nrog nplai ntawmCistanchedeserticolaYCma, uas muaj ntau yam tshuaj muaj txiaj ntsig. Nws cov decoction tuaj yeem nce qhov feem pua ​​​​phagocytic thiab phagocytic Performance index ntawm macrophages [2], thiab cov kev tshawb fawb ntxiv tau pom tias nws cov khoom siv hauv paus yog Cistanche deserticola polysaccharide (CDPS) [3], Zeng Qunli li al. [4] pom CDPS Ib qho kev tivthaiv ntawm thymus lymphocytes hauv vitro muaj qhov cuam tshuam loj heev rau nas thymus lymphocytes. Kab lus no saib xyuas cov teebmeem ntawm CDPS ntawm (spleen) lymphocyte proliferation hauv vivo thiab hauv vitro.

Cistanchepolysaccharidemuaj cov nyhuv ntawm kev txhim khotiv thaiv kab mob

Kev sib tham

ConA thiab LPS induced proliferation ntawm nas splenic lymphocytes yog nyob rau hauv vitro thwmsim los xyuas txog cov kev ua ntawm T thiab B lymphocytes nyob rau hauv lub cev, raws li, thiab yog ib qho tseem ceeb qhia kom muaj kev tiv thaiv tshwj xeeb. Cov kev tshawb fawb tau pom tias thaum qhov kawg siab ntawm CDPS siab dua 25 mgL, nws tuaj yeem txhawb kev loj hlob ntawm lymphocytes, qhia tias nws muaj qee yam mitogenic nyhuv. Nyob rau hauv lub xub ntiag ntawm mitogens (ConA los yog LPS), ib koob tshuaj me me ntawm CDPS tuaj yeem pom pom kev nthuav dav-txhim kho kev ua haujlwm. Peb paub tias ConA thiab LPS feem ntau ua rau kev loj hlob ntawm T lymphocytes lossis B lymphocytes, feem. Cov kev tshawb fawb tau pom tias CDPS tuaj yeem tsis tsuas yog txhawb kev loj hlob ntawm T lymphocytes, tab sis kuj txhawb kev loj hlob ntawm B lymphocytes, tab sis nws muaj kev cuam tshuam loj rau kev loj hlob ntawm B lymphocytes. Rau T lymphocytes.

Cistanche Polysaccharides: Txhim khoKev tiv thaiv

Lub thymus thiab spleen yog lub cev tiv thaiv kab mob tseem ceeb hauv cov tsiaj. Cov tshuaj tiv thaiv kab mob hauv tus po yog cov kab mob B lymphocytes uas paub tab, thiab cov thymus feem ntau tsis paub tab T lymphocytes. Lub thymus thiab spleen Performance index tuaj yeem cuam tshuam txog kev txhim kho ntawm lub cev tiv thaiv kab mob thiab kev ua haujlwm ntawm lub cev tiv thaiv kab mob. Kev txhaj tshuaj Intraperitoneal ntawm CDPS tuaj yeem ua rau nce tus po index ntawm cov nas ib txwm muaj thiab tiv thaiv kab mob, thiab nws kuj muaj qhov cuam tshuam loj rau nas thymic atrophy tshwm sim los ntawm Cy. CDPS tsis muaj qhov cuam tshuam tseem ceeb rau thymus Performance index ntawm cov tsiaj ib txwm, uas zoo ib yam nrog cov txiaj ntsig ntawm kev sim hauv vitro uas CDPS txhawb kev loj hlob ntawm B lymphocytes dua li T lymphocytes.

Cistanchepolysaccharide: nce kev loj hlob ntawmlymphocytesthiab txhim khotiv thaiv kab mob

IL-2 yog cytokine tsim los ntawm T lymphocytes los ntawm G1 theem ntawm lub voj voog ntawm tes mus rau theem S. Nws yog ib qho tseem ceeb ntawm T cell loj hlob thiab ua raws li kev loj hlob ntawm autocrine. Tsis tas li ntawd, IL-2 tuaj yeem txhawb B cell proliferation thiab antibodies Synthesis [11, 12]. CDPS txhawb kev tso tawm ntawm IL-2 los ntawm lymphocytes, thiab cov nyhuv no pom tseeb muaj feem xyuam rau CDPS kev txhawb nqa ntawm splenic lymphocyte proliferation.