Kev txiav txim siab ntawm Tag Nrho Saponins Cov ntsiab lus hauv Boschniakia Rossica Thiab Herba Cistanches Los Ntawm Spectrophotometry Ⅰ

Abstract:

Lub Hom Phiaj Lub hom phiaj no yog tsim los ntawm tag nrho cov saponins hauv Boschniakia rossica thiabHerba cistanchesthiab cov ntsiab lus ntawm covtag nrho saponins raug txiav txim. Txoj kev Salidroside raug xaiv los ua cov khoom siv, cov tshuaj tiv thaiv xim yog siv nrog vanillin thiab perchloric acid. Tom qab ntawd cov ntsiab lus ntawm tag nrho cov saponins hauv Boschniakia rossica thiab Herba cistanches tau txiav txim siab ntawm 523nm los ntawm spectrophotometric. Cov txiaj ntsig ntawm salidroside nkhaus yog linear tshaj qhov ntau ntawm 0. 05133 - 0. 2567mg·ml 1 nrog rau kev sib raug zoo ntawm 0. 9992, thiab cov kab rov tav kab rov tav yog Y=1. 611x 9. 0573. Rau Boschniakia rossica, qhov nruab nrab rov qab ntawm salidroside yog 99. 68% nrog SD ntawm 1. 68% (n=6); thiab rau Herba nrug, qhov nruab nrab rov qab ntawm salidroside yog 101. 18% nrog SD ntawm 0. 94% (n=6). Xaus Txoj kev yog qhov tseeb, yooj yim, thiab txhim khu kev qha. Yog li nws tuaj yeem siv los txiav txim siab cov ntsiab lus ntawm tag nrho cov saponins hauv Boschniakia rossica thiab Herba cistanches;

Ntsiab lus:Boschniakia rossica;Herba cistanches; Tag nrho saponins; Kev txiav txim siab; Spectrophotometric; Salidroside

Nws yuav siv sij hawm ntev npaum li cas rau CISTANCHE ua haujlwm?

Cistanche deserticola Y. C. Ma thiab Bosehniakia rossica Fedtseh et Flerov yog cov kab mob parasitic tshuaj nroj tsuag ntawm tsev neeg Orobanchiaceae. Cistanche deserticola yog cov kab mob cab uas nyob ntawm cov hauv paus hniav ntawm Haloxylon ammodendron, tsob ntoo suab puam. Nws yog tsim nyob rau hauv Xinjiang thiab Alxa League, Inner Mongolia. Nws yog lub npe hu ua "desert ginseng" vim nws tsis tshua muaj txiaj ntsig thiab muaj txiaj ntsig zoo tshuaj. Nyom [1] yog tsim nyob rau sab qaum teb sab hnub tuaj ntawm kuv lub teb chaws [2]. Raws li cov lus dab neeg, nws muaj txiaj ntsig ntawm kev tsis txawj tuag, yog li nws tau hu ua Northeast "Eternal Age Grass". Nws yog siv rau impotence vim lub raum tsis muaj peev xwm, mob khaub thuas nyob rau hauv lub duav thiab lub hauv caug, dryness ntawm txoj hnyuv, thiab cem quav. Nws tuaj yeem noj tau thiab ntxiv dag zog rau lub cev, ncua kev laus, thiab yog qhov muaj txiaj ntsig zoo hauv Northeast [3]. Ob leeg muaj cov txiaj ntsig zoo sib xws, thiab ob qho tib si muaj cov txiaj ntsig ntawm tonifying lub raum, ntxiv dag zog rau yang, moistening cov hnyuv, thiab laxative [4]. Raws li cov ntaub ntawv hais txog Cistanche deserticola nyob rau hauv Li Shizhen's "Compendium of Materia Medica" ntawm Ming Dynasty, "Cov tshuaj no yog tonic tab sis tsis hnyav, yog li nws yog hu ua calm. Nws tsis tshua tau txais los ntawm Congrong, yog li nws tau ntim nrog. Cistanche deserticola." Qhov ntawd yog hais tias, Cistanche deserticola ib zaug tau siv los ua lwm yam khoom siv tshuaj rau Cistanche deserticola. , Kev tshawb fawb niaj hnub no tau pom tias ob qho tib si Cistanche deserticola thiab Estuary deserticola muaj cov tshuaj pharmacological xws li tiv thaiv kev laus [5, 6], tiv thaiv qaug zog [7, 8], txhim kho kev tiv thaiv kab mob [9, 10], thiab txhim kho kev nco [{{ 12}}] ib.

Cov ntsiab lus ntawm tag nrho glycosides hauv Grasshopper thiab Cistanche deserticola muaj txiaj ntsig ntawm kev tshawb fawb tob. Ob leeg muaj saponins, volatile roj, suab thaj, amino acids, thiab lwm yam khoom. Cov kws tshawb fawb tau tshaj tawm cov kev tshawb fawb sib piv ntawm cov khoom ntawm cov roj tsis hloov pauv, suab thaj, amino acids, thiab lwm yam khoom ntawm ob [4, 14, 15], tab sis kev txiav txim siab ntawm tag nrho cov ntsiab lus glycoside ntawm ob tsis tau qhia hauv cov ntaub ntawv. . Cov khoom tseem ceeb ntawm Cistanche deserticola yog phenylpropanoid glycosides (series ntawm glucosides A, B, C, D, E, F, G, H, I, J, K) [16], thiab Cistanche deserticola yog phenylethanoid glycosides (series ntawm Cistanche deserticola). )[17,18], saponins ntawm ob qhov zoo sib xws, thiab lawv sib koom ua ke zoo sib xws, salidroside. Peb ntseeg tias glycosides yog cov khoom xyaw tseem ceeb ntawm ob qho tib si, thiab peb tshawb nrhiav kev siv spectrophotometric los txiav txim siab cov ntsiab lus ntawm tag nrho glycosides. , muab lub hauv paus theoretical rau kev txhim kho ntxiv thiab kev siv Echinacea deserticola thiab Cistanche deserticola cov peev txheej.

1 Cov khoom siv thiab cov cuab yeej siv

1. 1 Khoom thiab reagents: Amaranth officinale, sau los ntawm ib ncig ntawm Changbai Roob, txheeb xyuas raws li ib tug nroj tsuag ntawm tsev neeg Orobanaceae

Tag nrho cov nroj tsuag qhuav ntawm Orobanche coerulescens, ntxuav cov qauv, thiab ntws dej

Sib cais, muab tso rau hauv ib lub thawv kub kom qhuav ntawm 40 degree, qhuav kom qhov hnyav tas li, zuaj, hla dhau 40 mesh sieve thiab npaj.

[19]; Cistanche deserticola, Hohhot Enhe Trading Co., Ltd., txheeb xyuas raws li teev tseg

Cistanche deserticola Y. C. Ma cov nqaij qhuav

Stems, ntxuav, cua qhuav, qhuav kom qhov hnyav tas li, crushed, dhau los ntawm 40-mesh sieve, tso tseg; liab

Crassuside siv tshuaj (muab los ntawm Tuam Tshoj lub koom haum ntawm Tshuaj thiab cov khoom siv lom neeg, batch tooj 110818

- 200404, rau kev txiav txim siab cov ntsiab lus); tag nrho cov kev sim tshuaj reagents siv yog cov qhab nia ntsuam xyuas hauv tsev.

1. 2 Instruments 752N UV-pom spectrophotometer (Shanghai Jingke); Tu-1901 khub

Beam UV spectrophotometer (Beijing Puxi General Co., Ltd.); hluav taws xob analytical tshuav nyiaj li cas

AL104 1/100,000 (Mettler-Toledo Instrument Shanghai Co., Ltd.); FW-80

High-speed universal crusher (Shandong Hualu Electric Cua sov Instrument Co., Ltd.).

2 txoj kev

2. 1 Kev npaj cov kev daws teeb meem

Siv qhov ntsuas ntsuas kom raug ntsuas qhov hnyav salidroside 15. 40mg, yaj hauv methanol hloov mus rau 25-volumetric flask, thiab kho qhov ntim rau lub cim. Npaj kom muaj 0.0% salidroside ib ml. Cov tshuaj 616 mg yog siv los ua cov tshuaj siv thiab muab ntim rau hauv lub tub yees rau kev siv tom qab.

2. 2 Kev npaj cov tshuaj ntsuam xyuas

Siv qhov kev ntsuas ntsuas kom raug ntsuas cov hmoov tshuaj ntawm Grasshopper thiab Cistanche deserticola. 2. 0g, muab tso rau hauv ib lub thoob-bottomed flask, ntxiv 40ml ntawm 90% ethanol, tshav kub thiab reflux extraction ntawm Grasshopper ib zaug, thiab tshav kub thiab reflux Cistanche deserticola ob zaug, txhua lub sij hawm extraction yog 1. 5h, tom qab. suction filtration, muab cov filtrate, mloog zoo rau dryness nyob rau hauv ib da dej da dej, ntxiv 25ml dej kom yaj, extract 3 zaug nrog roj av ether, 25ml txhua lub sij hawm, muab pov tseg cov roj av ether kua nyob rau hauv lub Upper txheej, thiab ces mus ntxiv 3 zaug. nrog dej-saturated n-butanol. 25 ml txhua lub sijhawm, muab peb cov n-butanol rho tawm, evaporate cov n-butanol kua hauv lub da dej hauv lub fume hood, dilute nws nrog methanol rau hauv 10ml volumetric hwj, sib tov zoo, ces ntsuas 1ml ntawm cov tshuaj thiab dilute nrog. methanol rau 25 ml ntim. lub raj mis, sib tov kom zoo, thiab siv nws los ua kev sim tshuaj rau kev siv tom qab.

2. 3. Xaiv qhov ntsuas wavelength

yuav 1. Muab 25ml ntawm cov kev siv tshuaj thiab cov tshuaj ntsuam xyuas rau hauv cov tub ntxhais kawm tiav nrog cov stoppers, evaporate cov methanol hnyav nyob rau hauv ib tug tas li kub dej da dej ntawm 60 degree , thiab ntxiv 0. 2 ml ntawm 5% vanillin-glacial acetic acid tov thiab 0. 8 ml ntawm perchloric acid daws tau tshwm sim hauv 60 degree dej da dej rau 15 min. Tshem tawm lub raj xa dej thiab txias tam sim ntawd nrog dej khov rau 5 feeb. Ntxiv 5 ml ntawm glacial acetic acid thiab co zoo [20]. Npaj cov tshuaj siv tshuaj methanol tib yam. Cov kev daws teeb meem tom qab xim kev loj hlob tau raug xa mus rau wavelength scanning, thiab qhov siab tshaj plaws nqus wavelength ntawm 523 nm tom qab xim cov tshuaj tiv thaiv salidroside raug xaiv ntawm 400 thiab 800 nm raws li kev ntsuas wavelength ntawm tag nrho glycosides ntawm Escherichia coli thiab Cistanche deserticola. Cov qauv scanning ntawm cov tshuaj tswj thiab cov tshuaj ntsuam xyuas tau qhia hauv daim duab 1.

2. 4. Kev soj ntsuam ntawm kev sib raug zoo linear: Siv lub pipette los kos cov lus qhia kom raug 0. 5,1 ib. 0,

1.5, 2. 0, 2. 5ml tau muab tso rau hauv cov hlab ntsha uas tsis muaj npe 1 txog 5, thiab cov tshuaj tiv thaiv xim tau ua raws li cov kauj ruam hauv "2. 3". Ib qho kev sim dawb tau ua nrog methanol nyob rau hauv cov xwm txheej sib npaug, thiab qhov nqus tau raug ntsuas ntawm lub wavelength ntawm 523nm. Lub absorbance A (Y) yog ordinate, concentration mg·ml-1

(X) yog abscissa, linear regression yog ua, thiab qhov sib npaug regression yog Y {{{0}}. 611x 2. 0573 ( r=0. 9992), qhov tshwm sim qhia tau hais tias huab hwm coj concentration ntawm salidroside yog nyob ntawm 0. Txoj kev sib raug zoo yog zoo nyob rau hauv qhov ntau ntawm 05133-0.2567mg·ml-1. Cov txiaj ntsig tau pom hauv daim duab 2.

2. 5 Kev kuaj kev ruaj ntseg

Precisely ntsuas qhov tsim nyog tus nqi ntawm cov tshuaj ntsuam xyuas ntawm Grasshopper thiab Cistanche deserticola, tsim cov xim raws li txoj kev nyob rau hauv "2.3", thiab ntsuas tus nqi absorbance tusyees nyob rau hauv 6 lub sij hawm nyob rau hauv 30 feeb. Qhov no qhia tau hais tias tsis pub dhau 30 feeb tom qab kev tsim cov xim, Grasshopper thiab Cistanche deserticola yuav ruaj khov ntawm cov tshuaj ntsuam xyuas yog qhov zoo. Cov txiaj ntsig tau pom nyob rau hauv Table 1.

2. 6 Precision xeem

Precisely ntsuas tus nqi tsim nyog ntawm kev siv tshuaj, tsim cov xim raws li txoj kev hauv qab "2.3" saum toj no, thiab ntsuas qhov nqus 6 zaug tsis tu ncua. Cov txiaj ntsig tau qhia hauv Table 2. Cov txiaj ntsig tau pom tias qhov tseeb ntawm qhov kev sim ntsuas no yog qhov zoo.

Daim duab 1 Nyias-txheej scanning qauv ntawm salidroside siv tshuaj (A), nyom nyom test tov (B) thiab cistanche deserticola test solution (C)

2. 7. Kev xeem rov ua dua.

Precisely hnyav 6 feem ntawm cov nyom nyom thiab cistanche deserticola hmoov, txhua feem muaj 2. 0g, tom qab cua sov thiab reflux extraction siv cov txheej txheem zoo extraction uas tau txiav txim siab, ua raws li cov kauj ruam hauv qab "2. 3" thiab ntsuas qhov absorbance ntawm lub wavelength ntawm 523nm. Cov txiaj ntsig ntsuas tau pom nyob rau hauv Table 3 thiab Table 4.

2. 8. Kev kuaj tus nqi rov qab.

Ua kom raug hnyav 6 cov qauv ntawm Grasshopper thiab Cistanche deserticola nrog paub qhov loj feem pua ​​​​ntawm tag nrho glycosides. Txhua tus qauv yog 12 mg. Ua kom raug ntxiv 2 ml ntawm salidroside siv tshuaj. Ua raws li "2. 2" Extract cov qauv hauv qab cov khoom, txiav txim siab tag nrho cov ntsiab lus glycoside tom qab kev tsim xim raws li cov khoom "2.3", thiab xam cov nqi rov qab ntawm cov qauv. Cov txiaj ntsig tau pom nyob rau hauv Table 5 thiab Table 6.