Kev txiav txim siab ntawm Rau Phenylethanoid Glycosides hauv Cistanche Tubulosa Thiab Cistanche Deserticola Los Ntawm QAMS
Apr 17, 2023
MA Xue-qin, DONG Yang-hong, GUO Qin, DING Hua (Lub Tsev Haujlwm Saib Xyuas Tshuaj, Lub Chaw Haujlwm Tseem Ceeb ntawm Hui Cov Tshuaj Kho Mob Niaj Hnub Nyoog, Lub Tsev Haujlwm Saib Xyuas Kev Kawm, Ningxia Engineering thiab Technology Ｒ Tshawb Fawb Center rau kev kho tshiab ntawm Hui Tshuaj, Ningxia Medical University, Yingchuan, Ningxia 750004, Suav teb)
Abstract: Lub Hom Phiaj Los tsim ib txoj hauv kev HPLC rau kev txiav txim siab ib txhij ntawm 6 lub tebchaw suav nrog echinacoside, cistanoside C, cistanoside A, acteoside, 2＇－ acetylacteoside thiab 6＇－ acetylacteoside hauv Cistanche tubulosa thiab Cistanche - deseritative ib qho kev tshuaj ntsuam xyuas (QAM). Txoj kev Los ntawm kev siv echinacoside raws li kev siv sab hauv, tus txheeb ze kho qhov tseem ceeb (ＲCF) ntawm cistanoside C, cistanoside A, acteoside, 2＇－ acetylacteoside thiab 6＇ acetylacteoside tau tsim, thiab saum toj no 6 phenylethanoid glycosides tau txiav txim los ntawm cov khoom siv thiab txheem nkhaus txoj kev, feem. Cov txiaj ntsig kev txheeb ze ntawm acteoside, cistanoside C, cistanoside A, 2＇ - acetylacteoside thiab 6＇ acetylacteoside raug xam los ntawm txoj kev siv tau raws li 1. 008,1. 871, 16. 809, 0. 611 ,ua 0. 603, ib. Cov txiaj ntsig kev txiav txim siab tau muab piv nrog cov ntaub ntawv tau txais los ntawm tus qauv nkhaus txoj kev uas qhia tau hais tias qhov zoo ntawm kev rov ua dua ntawm cov txheeb ze sib raug zoo, thiab tsis muaj qhov sib txawv tseem ceeb hauv kev soj ntsuam cov txiaj ntsig tau pom ntawm cov txheej txheem sab nraud thiab QAMS txoj kev. Xaus Txoj kev yog qhov yooj yim thiab txhim khu kev qha, uas tuaj yeem siv tau yooj yim rau kev sib txawv thiab txiav txim siab cov ntsiab lus ntawm ntau cov khoom siv bioactive hauv Cistanche tubulosa thiab Cistanche deserticola, nrog rau lawv cov kev tswj kom zoo.
Cov lus tseem ceeb: Tubulosa; Cistanche deserticola; Phenylethanoid glycosides; QAM; Kev tswj kom zoo
Cistanche deserticola
Cistanche deserticola YCMa los yog Cistanche tubulosa (Schenk) Xav, tshuaj suav tshuaj rau tonifying lub raum, tau sau tseg hauv 2015 tsab ntawm Suav Pharmacopoeia. Nws yog ib tug succulent qia ntawm genus Cistanche nyob rau hauv tsev neeg Lewandaceae, feem ntau faib nyob rau hauv Inner Mongolia, Ningxia, Gansu, Qinghai, thiab Xinjiang. Cistanche deserticola thiab Tubular Cistanche deserticola muaj txiaj ntsig zoo heev. Lawv nkag mus rau hauv lub raum thiab txoj hnyuv loj meridians. Lub Compendium ntawm Materia Medica sau tseg tias Cistanche deserticola "yog ib qho khoom noj tab sis tsis hnyav, yog li nws hu ua Cistanche deserticola" thiab "nws sov tab sis tuaj yeem moisten, rov ua dua tab sis tsis qhuav, du tab sis tsis raws plab".
Cstanche deserticola
Nws muaj cov teebmeem ntawm tonifying raum yang, muaj txiaj ntsig cov ntsiab lus thiab cov ntshav, moistening cov hnyuv, thiab defecation. Kev lig kev cai, nws yog siv rau raum yang deficiency, tsis muaj peev xwm ntawm cov ntsiab lus thiab cov ntshav, impotence, infertility, mob ntawm lub duav thiab lub hauv caug, tsis muaj zog ntawm cov leeg thiab pob txha, thiab cem quav vim plab hnyuv dryness, Nws yog cov feem ntau siv tonifying raum yang tshuaj. hauv keeb kwm. Kev tshawb fawb tshuaj niaj hnub no qhia tau hais tias Cistanche deserticola muaj ntau yam cuam tshuam xws li kev txhim kho kev sib deev, tiv thaiv kev laus, txhim kho kev kawm thiab kev nco muaj peev xwm, tiv thaiv Alzheimer's kab mob, defecation, thiab lwm yam. Nws yog dav siv nyob rau hauv kev kho mob tshuaj ntawm Suav tshuaj, tshuaj suav tshuaj patent tshuaj. , thiab kev npaj yooj yim thiab cov khoom kho mob [2-5].
Cistanche deserticola kev ua tau zoo-tonifying raum
Nyem qhov no mus saib Cistanche rau raum kab mob khoom
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Cov tshuaj tseem ceeb ntawm Cistanche deserticola yog phenylethanoid glycosides, iridoids thiab lawv cov glycosides, lignans, thiab polysaccharides [6]. Cov kev tshawb fawb tshuaj niaj hnub no tau pom tias phenylethanoid glycosides yog cov tshuaj tseem ceeb tshaj plaws hauv Cistanche deserticola, thiab muaj cov tshuaj tiv thaiv kab mob Alzheimer, Parkinson's disease, myocardial ischemia, thiab lwm yam. [7].
Cov tshuaj tseem ceeb ntawm Cistanche deserticola
Tam sim no, muaj ntau txoj hauv kev los tswj cov khoom xyaw zoo hauv Cistanche deserticola. Lub pharmacopeia tau tsim cov ntsiab lus kev txiav txim siab rau echinacoside thiab poolside hauv Cistanche deserticola, uas yog qhov nyuaj rau kev xav txog thiab tswj qhov zoo ntawm Cistanche deserticola. Nyob rau hauv xyoo tas los no, txawm hais tias muaj cov lus ceeb toom siv HPLC ib txhij txiav txim siab cov ntsiab lus ntawm plaub phenylethanoid glycosides hauv Cistanche deserticola.mav, ntau yam ntaub ntawv siv yuav tsum tau, uas ua rau kom tus nqi sim thiab daim ntawv thov. Txawm li cas los xij, tsis muaj cov ntaub ntawv qhia txog kev txiav txim siab ntau ntawm rau phenylethanoid glycosides hauv Cistanche deserticola, nrog rau cov txheej txheem rau kev txiav txim siab cov ntsiab lus ntawm Cistanche deserticola nrog ib tus qauv thiab ntau qhov kev ntsuam xyuas. Qhov kev sim no tau tsim kom muaj Kev Ntsuam Xyuas Ntau Yam los ntawm Ib Tus Neeg Tsim Khoom (QAMS) txoj hauv kev rau kev txiav txim siab ib txhij ntawm rau 6 lub luag haujlwm hauv Cistanche deserticola, suav nrog echinacoside, anthocyanin, glycoside C, glycoside A, 2-acetyl anthocyanin, thiab {{ 1}}acetyl anthocyanin, siv ib qho khoom siv ntawm echinacoside raws li kev siv. Nyob rau tib lub sijhawm, daim ntawv tau ua ib qho kev tshawb fawb sib piv ntawm cov ntsiab lus ntawm rau lub ntsiab lus uas tau hais los saum toj no hauv ob hom tshuaj ntsuab, Cistanche deserticola, thiab Cistanche tubulosa, txhawm rau tshawb xyuas qhov sib txawv ntawm cov khoom siv zoo ntawm Cistanche deserticola los ntawm ntau qhov chaw, thiab muab kev sim cov ntaub ntawv rau kev tswj kom zoo ntawm Cistanche deserticola.
1 Cov khoom siv thiab cov cuab yeej siv
Agilent 1260 high-performance kua chromatograph; Shimadzu UV-2550 UV spectrophotometer, Nyiv; Shanghai Jinpeng FW100 tsoos suav tshuaj Grinder; KQ-500E ultrasonic nqus tsev vacuum (Kunshan Ultrasonic Instrument Co., Ltd.); Eppendorf pipette (Lub teb chaws Yelemees); AL104 Analytical tshuav nyiaj li cas (Mettler Toledo Instrument Co., Ltd.); Cov dej distilled; Tag nrho lwm cov reagents yog tsom xam ntshiab. Echinacetin (batch tooj: 111670-200503) los ntawm Tuam Tshoj lub koom haum rau kev tswj cov khoom lom; Maorui paj glycoside (batch nab npawb: Yuav tsum-12030806, Tuam Tshoj lub koom haum rau kev tswj cov khoom lom); Cistanche glycoside C, Cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl pas dej ua ke yog txhua tus kheej tsim hauv chav kuaj ( txheeb xyuas los ntawm NMR thiab MS spectra), nrog HPLC purity ntau dua 95 feem pua ; Cov suab puam cistanche deserticola YCMa yog muab los ntawm Ningxia Yongning cistanche cog puag, thiab tubular cistanche tubulosa (Schenk) Wight yog muab los ntawm Ningxia Mingde Herbal Tablets Co., Ltd. Cov ntaub ntawv tshuaj saum toj no tau txheeb xyuas los ntawm Associate Professor Dong Lin ntawm Ningxia Medical College Pharmacy Teaching and Research Office. Methanol, acetonitrile (chromatographically ntshiab, TEDIA, USA); Wahaha Dej Ntshiab; Tag nrho lwm cov reagents yog tsom xam ntshiab.
Phenylethanol glycoside yog lub luag haujlwm tseem ceeb ntawm Cistanche deserticola
2 Cov txheej txheem thiab cov txiaj ntsig
2.1 Kev Ntsuam Xyuas Ntawm Ib Qhov Kev Ntsuas thiab Kev Ntsuas Ntau Yam
2.1.1 Kev npaj cov kev daws teeb meem
Siv qhov tsim nyog ntawm cov khoom qhuav qhuav rau qhov ntsuas qhov hnyav tas li ntawm echinacoside, pas dej ua ke, cistanche glycoside C, cistanche glycoside A, {{0}}acetyl pas dej, thiab 6-acetyl pas dej ua ke, ntsuas qhov tseeb, thiab ntxiv methanol los npaj cov tshuaj sib xyaw ua ke nrog cov concentrations ntawm 0.027, 0.{{10}}27, 0.008, 0.014, 0.022, thiab 0.007 mg · ml-1, raws.
2.1.2 Kev npaj cov tshuaj ntsuam xyuas
Noj li ntawm 4. methanol los ua kom poob phaus, lim lawv cais, noj 1 ml ntawm cov lim dej tom ntej, dilute 20 zaug, thiab tau txais.
2.1.3 Chromatographic tej yam kev mob
Agilent 1260 kua chromatography system; Chromatographic kem TSK GEL C18 kem (4.6 id × 250 mm; tsib μ m); Gradient elution nrog acetonitrile (B) -0.5 feem pua acetic acid dej (A): 0-10 feeb, 83 feem pua -80 feem pua A; 10 - 30 min, 80% - 75% A; 30 - 40 min, 75% - 70% A; Tus nqi ntws: 1.0 ml · min-1; Ntsuas wavelength 333 nm; Txhaj ntim: 5 μl; Chav ntsuas kub. Raws li cov xwm txheej saum toj no chromatographic, cov chromatograms ntawm cov khoom siv sib xyaw thiab cov tshuaj ntsuam xyuas tau pom nyob rau hauv daim duab 1-3, raws li.
1. Echinacetin; 2. Cistanche glycoside A; 3. Maorui paj glycoside; 4. Cistanche glycoside C; 5.2- Acetyl piloside; 6.6- Acetylpiloside
Daim duab 1 HPLC chromatogram ntawm kev siv tshuaj sib xyaw
1. Echinacetin; 2. Cistanche glycoside A; 3. Maorui paj glycoside; 4. Cistanche glycoside C; 5.2- Acetyl piloside; 6.6- Acetylpiloside
Daim duab 2 HPLC Chromatogram ntawm Desert Cistanche Test Solution
1. Echinacetin; 2. Cistanche glycoside A; 3. Maorui paj glycoside; 4. Cistanche glycoside C; 5 2- Acetyl piloside; 6.6- Acetylpiloside
Daim duab 3 HPLC chromatogram ntawm cov tshuaj ntsuam xyuas ntawm Cistanche deserticola
2.1.4 Linear kev sib raug zoo
Siv cov tshuaj sib xyaw saum toj no thiab siv 1, 2, 3, 4, 5, thiab 6, raws li μ Txhaj cov qauv rau kev kuaj pom raws li cov kab mob chromatographic saum toj no, thiab ua cov kab rov ua dua nrog cov qauv siv tshuaj siv (X) raws li x-axis thiab lub ncov cheeb tsam (Y) raws li y-axis. Cov txiaj ntsig tau qhia hauv Table 1, qhia tias kev sib raug zoo ntawm txhua tus qauv tswj tau zoo nyob rau hauv lawv qhov kev txhaj tshuaj.
Table 1 Linear kev sib raug zoo ntawm rau phenylethanoid glycoside siv tshuaj
2.1.5 Stability test
Siv tib cov tshuaj ntsuam xyuas thiab txhaj cov qauv ntawm 0, 2, 4, 8, thiab 12 teev tom qab kev npaj los txiav txim siab qhov chaw siab tshaj ntawm echinacoside, pas dej ua ke, cistanche glycoside C, cistanche glycoside A, 2- acetyl pas dej, thiab 6-acetyl pas dej ua ke. Muab xam RSDs ntawm 1.51 feem pua , 1.80 feem pua , 1.80 feem pua , 1.71 feem pua , 1.85 feem pua , thiab 1.82 feem pua , raws li qhia tau hais tias cov tshuaj ntsuam xyuas yog ruaj khov nyob rau hauv 12 teev.
2.1.6 Precision xeem
Siv cov tshuaj sib xyaw ua ke thiab rov txhaj tshuaj 6 zaug raws li cov xwm txheej saum toj no chromatographic. Txiav txim siab thaj tsam qhov tseem ceeb ntawm echinacoside, pas dej ua ke, cistanche glycoside C, cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside. Xam cov RSDs li 0.23 feem pua , 0.01 feem pua , 0.10 feem pua , 0.20 feem pua , 0.54 feem pua , thiab 0.32 feem pua , feem, qhia qhov tseeb ntawm qhov ntsuas.
2.1.7 Repeatability test
Ua kom raug hnyav 6 feem ntawm cov qauv kuaj hmoov ntawm Desert Cistanche deserticola, npaj cov tshuaj ntsuam xyuas raws li txoj hauv kev "2.1.2", thiab txhaj 5 cov qauv raws li cov qauv chromatographic saum toj no μ Qhov nruab nrab qhov tseem ceeb ntawm cov ntsiab lus ntawm echinacoside, poolside, cistanche glycoside C, cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside tau txiav txim siab yog 8.48, 8.25, 2.35, 1.49, 6.63, thiab 1.65 mg · g-1 , raws. Cov kev suav RSDs yog 1.58 feem pua, 1.44 feem pua, 1.58 feem pua, 1.59 feem pua, 1.05 feem pua, thiab 1.43 feem pua, qhia tau hais tias zoo repeatability ntawm txoj kev.
2.1.8 Tus qauv rov qab tus nqi
Noj 9 qhov chaw ntawm cov suab puam deserticola hmoov nrog cov ntsiab lus paub nyob rau hauv "2.1.7", 3 qhov chaw muab faib ua 1 pawg, thiab txhua feem yog 0.1 g, raug hnyav. Ua kom raug ntxiv txhua qhov kev siv tshuaj rau txhua pab pawg thiab suav qhov nruab nrab tus qauv rov qab los. Cov txiaj ntsig tau qhia hauv Table 2 hauv qab no.
Table 2 Rov qab tus nqi ntawm cov suab puam deserticola tom qab kuaj
2.2 Kev txiav txim siab txog kev kho tus txheeb ze
2.2.1 Kev txiav txim siab txog kev kho qhov txheeb ze rau txhua yam
Siv Echinacetin raws li cov ntaub ntawv siv thiab siv cov txheej txheem ntau lub ntsiab lus calibration [8-11], siv cov tshuaj sib xyaw ua ke hauv qab "2.1.1", txhaj cov qauv rau kev tshuaj xyuas, thiab sau cov cheeb tsam ncov ntawm txhua qhov khoom. Xam cov txheeb ze kho qhov tseem ceeb (fk/s) ntawm txhua qhov khoom raws li cov qauv hauv qab no, siv echinacoside ua cov khoom siv. Cov txiaj ntsig tau qhia hauv Table 3.
fk/s=fk /fs=( Ck × As) /( Cs × Ak )
Nyob rau hauv cov mis, Raws li yog lub ncov cheeb tsam ntawm cov ntaub ntawv siv s, Cs yog lub concentration ntawm cov ntaub ntawv siv s, Ak yog lub ncov cheeb tsam ntawm ib co Cheebtsam k yuav tsum tau kuaj, thiab Cs yog lub concentration ntawm ib co tivthaiv k.
Table 3 Cov txheeb ze kho qhov tseem ceeb fk/s rau txhua qhov siv Echinacetin ua cov khoom siv
Cov txheeb ze kho yam tseem ceeb rau lub pas dej, cistanche glycoside C, cistanche glycoside A, {{0}}acetyl pas dej, thiab 6-acetyl pas dej yog 1.008, 1.871, 0.809, 0.611, thiab 0.603, feem, nrog RSD tsawg dua 1.5 feem pua.
2.2.2 Qhov cuam tshuam ntawm ntau qhov kev txhaj tshuaj sib txawv ntawm f
Kev tshuaj xyuas cov ntim sib txawv (1, 2, 3, 4, 5 μ l) Raws li qhia hauv Table 4, cov txheeb ze kho qhov tseem ceeb f rau ntau qhov kev txhaj tshuaj ntawm pas dej, cistanche glycoside C, cistanche glycoside A, 2-acetyl poolside , thiab 6-acetyl poolside yog 1.80 feem pua , 2.26 feem pua , 1.46 feem pua , 2.99 feem pua , thiab 3.27 feem pua , raws li qhia tau hais tias cov tshuaj sib txawv tsis muaj qhov cuam tshuam loj rau f ntawm txhua qhov khoom.
2.2.3 Cov nyhuv ntawm cov kab chromatographic sib txawv ntawm f
Peb tau tshawb xyuas qhov cuam tshuam ntawm plaub kab chromatographic (YMC-Triart C18, Agilent Eclipse 5 XDB-C18, TOSOH TSK GEL ODS-100V, thiab Agilent ZORBAX SB-C18) ntawm txhua yam. Cov txheeb ze kho yam tseem ceeb f rau poolside, cistanche glycoside C, cistanche glycoside A, 2-acetyl pas dej ua ke, thiab 6-acetyl pas dej ua ke nyob rau hauv sib txawv chromatographic kab yog 2.55 feem pua, 1.89 feem pua, thiab 3.45 feem pua, feem, 2.49 feem pua thiab 2.21 feem pua (Table 4) qhia tias cov kab chromatographic sib txawv tsis muaj qhov cuam tshuam loj rau f ntawm txhua qhov.
2.2.4 Kev cuam tshuam ntawm cov cuab yeej sib txawv ntawm f
Cov teebmeem ntawm peb txoj kev HPLC sib txawv (Agilent 1220, Agilent 1260, Agilent 1260 II) ntawm txhua qhov f tau tshawb xyuas. Raws li pom nyob rau hauv Table 4, cov txheeb ze kho yam tseem ceeb f rau poolside, Cistanche C, Cistanche A, 2-acetyl poolside, thiab 6-acetyl pas dej ua ke nyob rau hauv ntau yam twj paj nruag yog 1.69 feem pua, 2.52 feem pua, 1.66 feem pua, 2.06 feem pua , thiab 2.26 feem pua , raws li (Table 4), qhia tias cov twj paj nruag sib txawv tsis muaj kev cuam tshuam loj rau f ntawm txhua qhov khoom.
2.2.5 Qhov cuam tshuam ntawm cov neeg ua haujlwm sib txawv ntawm f
Qhov cuam tshuam ntawm peb tus neeg sib txawv ntawm txhua tus f tau tshawb xyuas, raws li qhia hauv Table 4. Cov txheeb ze kho qhov cuam tshuam f rau lub pas dej, cistanche glycoside C, cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside yog 1.80 feem pua , 2.26 feem pua , 1.46 feem pua , 2.99 feem pua , thiab 3.27 feem pua , raws li, ntawm qhov sib txawv txhaj tshuaj ntim. Qhov no qhia tau hais tias ntau qhov kev txhaj tshuaj sib txawv tsis muaj qhov cuam tshuam loj rau f ntawm txhua qhov khoom.
Table 4 Qhov cuam tshuam ntawm ntau yam ntawm cov txheeb ze kho qhov fk/s ntawm txhua qhov khoom
2.3 Kev txiav txim siab txog lub sij hawm tuav tus txheeb ze ntawm txhua qhov khoom
Lub sij hawm tuav cov txheeb ze ntawm cov khoom ntawm lub pas dej, cistanche glycoside C, cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside yog 1.91, 2.60, 1.42, 2.99, thiab 3.{{11} }, feem, nrog RSD tsawg dua 1.5 feem pua. Lub repeatability tau zoo.
2.4 Kev sib piv ntawm kev ntsuas cov txiaj ntsig ntawm ib qho kev ntsuas ntau txoj kev ntsuas thiab cov txheej txheem sab nraud
Noj kwv yees li 4. Siv cov txheej txheem sab nraud los txiav txim siab cov ntsiab lus ntawm echinacoside, poolside, cistanche glycoside C, cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside, thiab tom qab ntawd suav cov ntsiab lus siv QAMS tsim. Cov txiaj ntsig tau pom nyob rau hauv Table 5.
Table 5 Simultaneous Txiav txim siab ntawm Rau Phenylethanol Glycosides nyob rau hauv Cistanche deserticola thiab Cistanche tubulosa los ntawm Sab Nraud Standard Method thiab QAMS Method mg · g-1
- " txhais tau tias tsis pom; n=3
Raws li qhia hauv Table 5, qhov sib txawv ntawm qhov sib txawv ntawm qhov ntsuas qhov tseem ceeb ntawm tus qauv sab nraud thiab cov txiaj ntsig ntawm QAMS yog tag nrho hauv 3 feem pua, qhia tias tsis muaj qhov sib txawv tseem ceeb hauv kev ntsuas ntawm ob txoj hauv kev. Txoj kev QAMS tau tsim los rau kev soj ntsuam zoo ntawm ntau yam phenylethanoid glycosides hauv Cistanche deserticola.
3 Kev sib tham
2015 tsab ntawm Suav Pharmacopoeia tau teev tseg tias tag nrho cov nyiaj ntawm echinacoside thiab pas dej hauv Cistanche deserticola yuav tsum tsis pub tsawg dua 0.3 feem pua, thiab cov ntsiab lus ntawm ob qho tib si hauv Cistanche tubulosa yuav tsum tsis pub tsawg dua 1.5 feem pua. Phenylethanolamine glycosides, raws li ib tug tseem ceeb active tivthaiv nyob rau hauv Cistanche deserticola, muaj nyob rau hauv cov ntsiab lus siab, ntxiv rau echinacoside thiab anthocyanins. Lwm cov khoom xws li Cistanche deserticola glycoside C, Cistanche deserticola glycoside A, 2-acetyl anthocyanins, thiab 6-acetyl anthocyanins kuj tseem muaj cov ntsiab lus siab. Cov kev tshawb fawb tau pom tias Cistanche glycoside A tuaj yeem muaj kev tiv thaiv tseem ceeb ntawm cov pa roj carbon tetrachloride thiab cawv ua rau lub siab raug mob hauv cov nas, thiab tuaj yeem txhim kho cov dawb radical clearance rate. Tag nrho cov phenylethanoid glycosides ntawm Cistanche deserticola muaj cov tshuaj tiv thaiv kab mob thiab antioxidant. Tsis tas li ntawd, kev tshawb fawb tau pom tias cov ntsiab lus ntawm Cistanche glycoside A thiab 2-acetyl poolside nyob rau hauv cov tshuaj ntsuab tau sau rau lub caij nplooj ntoos hlav yog qhov siab, thiab ob qho khoom siv tau zoo no muaj cov haujlwm tshuaj zoo sib xws li echinacoside thiab poolside. Nws raug nquahu kom ntxiv Cistanche glycoside A thiab 2-acetyl poolside rau qhov ntsuas qhov ntsuas rau kev txiav txim siab cov ntsiab lus ntawm Cistanche [15]. Yog li, tsuas yog ntsuas cov ntsiab lus ntawm echinacoside thiab poolside tsis tuaj yeem cuam tshuam tag nrho cov teebmeem tshuaj thiab tshuaj zoo ntawm Cistanche deserticola. Qhov kev sim no siv ib qho kev ntsuam xyuas ntau txoj hauv kev, siv Echinacetin ua cov ntaub ntawv siv, los tsim cov txheeb ze kho qhov tseem ceeb ntawm Cistanche glycoside C, Cistanche glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside nrog Echinacetin.
Cistanche extract hmoov
Los ntawm kev txiav txim siab ntawm ib qho khoom siv ntawm Echinacetin, tag nrho ntawm rau phenylethanoid glycosides nyob rau hauv cov khoom siv tshuaj tau raug soj ntsuam zoo thiab ntau npaum li cas, yog li ua kom paub tseeb qhov zoo ntawm cov khoom siv tshuaj.
Qhov kev sim tau tshawb xyuas qhov cuam tshuam ntawm qhov sib txawv ntawm cov ntim ntim, cov kab chromatographic, cov cuab yeej, thiab cov neeg ua haujlwm sim ntawm cov txheeb ze kho qhov cuam tshuam, thiab cov txiaj ntsig tsis pom qhov txawv txav (RSD)<5%); The QAMS method was validated using the external standard method, and the results showed that there was no significant difference between the calculated values of the QAMS method and the measured values of the external standard method, indicating that the relative correction factor established in this experiment is accurate and reliable.
Tsis tas li ntawd, cov txiaj ntsig kev sim kuj tau pom tias muaj qhov sib txawv tseem ceeb hauv cov ntsiab lus ntawm 6 phenylethanolamine glycosides hauv cov suab puam thiab tubular deserticola deserticola, thiab tseem muaj qhov sib txawv tseem ceeb ntawm cov ntsiab lus ntawm cov khoom xyaw zoo hauv tib cov khoom siv tshuaj los ntawm cov khoom sib txawv. , uas yog ze ze rau hom khoom siv tshuaj, ib puag ncig kev loj hlob, thiab lub sijhawm sau qoob. Hauv cov ntsiab lus, qhov kev sim no tau tsim ib txoj hauv kev los txiav txim siab cov ntsiab lus ntawm Cistanche deserticola nrog ntau txoj kev ntsuas. Los ntawm kev siv ib yam khoom siv, cov ntsiab lus ntawm 6 phenylethanoid glycosides hauv Cistanche deserticola thiab Cistanche deserticola tuaj yeem raug xam kom raug, suav nrog echinacoside, pas dej, glycoside C, glycoside A, 2-acetyl poolside, thiab 6-acetyl poolside , Ntxiv mus, nws tau ua tiav ntau qhov ntsuas cov ntsiab lus kev txiav txim siab nyob rau hauv cov ntaub ntawv ntawm qhov tsis txaus ntawm cov ntaub ntawv siv, muab ntau txoj kev tshawb fawb thiab yooj yim rau kev tswj xyuas zoo ntawm Cistanche deserticola.
siv
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