Cistanoside Ntawm Cistanche Herba Ameliorates Hypoxia-induced Txiv neej Reproductive Damage Via Suppression Of Oxidative Stress-Ⅱ

Cov teebmeem ntawm Cis ntawm hypoxia-induced GC-1 cell viability in vitro.

Txhawm rau tshawb xyuas seb Cis tuaj yeem tiv thaiv qhov cuam tshuam inhibitory ntawm hypoxia ntawm GC{{0}} cell viability, CCK-8 kev soj ntsuam tau ua. GC-1 hlwb raug kho nrog ntau hom subtypes (Cis-A, B, C, H) thiab ntau qhov concentration (0.02 μM, 0.2 μM, 2 μM) ntawm Cis rau 72 h. Kev sib piv ntawm pawg qauv nrog pawg DMSO tau pom tias DMSO tsis ncaj qha txhawb GC{{10}} cell viability (Daim duab 2A). Txawm li cas los xij, cell viabilities tau rov qab zoo heev (P < 0.05) nrog Cis kev kho mob. Piv nrog rau pawg qauv, Cis-A, Cis-B, Cis-C, thiab Cis-H txhua tus tau pom qee yam kev tiv thaiv ntawm hypoxia-vim kev puas tsuaj rau GC-1 cell viability, thiab Cis-B pom qhov tseem ceeb tshaj plaws. cov nyhuv (Daim duab 2A). Kev tiv thaiv los ntawm Cis ntawm 0.2 μM tau nce siab dua li cov kev tiv thaiv ntawm Cis ntawm 0.02 μM, thaum qhov sib txawv ntawm 2 μM thiab 0.2 μM tsis pom tseeb, qhia tias qhov rov qab los. GC-1 cell viability induced by Cis demonstrated dose-dependent in the concentration range from 0.02-0.2 μM (Daim duab 2A). Yog li ntawd, raws li kev xav tau ntawm kev sim, 0.2 μM Cis tau raug xaiv los ua qhov kev pom zoo hauv cov kev sim hauv vitro hauv qab no. Txhawm rau kom paub meej ntxiv seb cov kab mob puas muaj tseeb tiv thaiv los ntawm Cis, FCM, thiab Ki-67 staining tau ua los ntsuas qhov kev hloov pauv ntawm kev loj hlob ntawm GC-1 hlwb tom qab kho nrog Cis. Raws li kev kho Cis, qhov kev faib ua feem ntawm GC-1 hlwb hauv G1 theem tau txo qis. Hauv qhov sib piv, ntau lub hlwb nkag mus rau theem S, qhia tias Cis-kev kho mob tuaj yeem ua rau cov kab mob cell proliferation index (P <0.01; Daim duab 2B). Cov txheeb cais rau GC-1 lub voj voog ntawm tes tau qhia hauv daim duab 2Bb. Cov txiaj ntsig ntawm Ki-67 staining kuj tau pom tias Cis-A, Cis-B, Cis-C, thiab Cis-H kho tau zoo txhim kho Ki-67- qhov zoo ntawm cell ratio ntawm hypoxia-induced GC{{54} } hlwb hauv vitro (Daim duab 2C).

NATURAL CISTANCHE TUBULOSA rau kev txhim kho kev sib deev PHGS75% ECH 30% ACT 12%

Cov txheej txheem ntawm Cis tiv thaiv cov kab mob los ntawm hypoxia hauv vitro.

Txhawm rau tshawb xyuas seb qhov kev tiv thaiv ntawm Cis ntawm GC-1 cov hlwb puas muaj feem cuam tshuam nrog kev tshem tawm ROS ntau dhau, cov tshuaj fluorescent DCFH-DA tau siv los txheeb xyuas cov qib ROS hauv txhua pab pawg. Raws li pom nyob rau hauv daim duab 3A, thiab 3B, kev kho mob nrog DMSO tsis hloov cov ntsiab lus ntawm intracellular ROS los yog qib LPO piv nrog cov qauv pawg. Txawm li cas los xij, ROS qib hauv GC-1 hlwb tau txo qis hauv cov pab pawg Cis kho (Daim duab 3A). Tsis tas li ntawd, qhov txo qis hauv LPO kuj tau pom hauv GC-1 cov hlwb raug rau Cis (Daim duab 3B).

Txhawm rau tshawb nrhiav ntxiv cov txheej txheem uas Cis tiv thaiv cov kab mob los ntawm kev raug mob hypoxic, TUNEL staining, thiab Western blot tsom xam tau ua los ntsuas apoptosis. TUNEL staining (Daim duab 3C) tau pom qhov tseem ceeb apoptosis hauv cov qauv thiab DMSO pawg. Txawm li cas los xij, tsawg dua cov hlwb apoptotic tau pom nrog kev kho Cis, uas qhia tias kev kho Cis txo GC-1 cell apoptosis. Tsis tas li ntawd, qhov kev qhia ntawm PARP, Caspase-3, Bax, thiab Bcl-2 raug ntsuas los ntsuas cov txheej txheem molecular. Raws li tau hais hauv daim duab 3D, Caspase-3 thiab PARP tau qhib rau hauv GC-1 cov hlwb hauv qab hypoxia, thiab qhov kev ua kom no tau cuam tshuam los ntawm Cis kev kho mob. Tsis tas li ntawd, qhov piv ntawm Bax/Bcl-2 yog siab dua nyob rau hauv cov qauv pab pawg tshaj li nyob rau hauv pawg tswj, thiab Cis kev kho mob txo tus piv ntawm Bax/Bcl-2 (Daim duab 3D). Cov ntaub ntawv no tau qhia tias Cis muaj lub peev xwm los txo qis kev puas tsuaj ntawm hypoxia-induced oxidant, thiab qhov kev tiv thaiv no yuav ua tiav los ntawm kev txo qis ROS tsub zuj zuj thiab inhibiting Caspaserelated apoptosis txoj kev ua kom.

Lub tshuab enzymatic inhibiting OS suav nrog cov dawb radical scavengers xws li glutathione reductase (GR), glutathione peroxidase (GPx), thiab superoxide dismutase (SOD) [23]. Lub tshuab enzymatic inhibiting OS plays lub luag haujlwm tseem ceeb hauv kev tiv thaiv oxidative puas hauv hlwb thiab cov ntaub so ntswg [23]. Txhawm rau ua kom muaj txiaj ntsig ntxiv rau lub peev xwm ntawm Cis inhibition ntawm hypoxia-induced OS hauv GC-1 hlwb, cov haujlwm ntawm GR, GPx thiab SOD raug ntsuas. Cov txiaj ntsig tau nthuav tawm tias GR, GPx, thiab SOD cov dej num txhua qhov tseem ceeb (P < 0 01, Daim duab 3E) poob qis hauv hypoxia thaum piv rau pawg tswj hwm, thiab Cis kev kho mob tau rov qab zoo dua lawv cov dej num hauv GC-1 hlwb raug rau hypoxia (P < 0.05, daim duab 3E), qhia tias cov tebchaw no tuaj yeem qhib lub zog endogenous antioxidant system.

CISTANCHE TUBULOSA RAU STIMULATING SPERM PRODUCTION PHGS75% ECH 30% ACT 12%

Qhov cuam tshuam ntawm Cis ntawm kev yug me nyuam hauv cov nas hypobaric hypoxia-induced nas.

Txhawm rau txiav txim siab qhov cuam tshuam ntawm hypobaric hypoxia ntawm cov txiv neej nas, peb thawj zaug sim morphological alterations ntawm testes hauv hypobaric hypoxia-induced nas. Cov txiaj ntsig ntawm HE staining pom tias nyob rau hauv pawg tswj hwm, cov kab mob spermatogenic ib txwm nyob ntawm ntau theem tau npaj kom zoo los ntawm cov kab hauv qab daus mus rau lumen, thiab cov phev laus tau pom hauv tubule lumens (Daim duab 4A). Piv nrog rau kev tswj hwm, kev hloov pauv pathological ntawm cov qog nqaij hlav hauv cov poj niam cev xeeb tub tau pom nyob rau hauv pawg qauv, hauv qab daus daim nyias nyias ntawm cov qe ntshav epithelial tau teeb tsa xoob, cov spermatogenic epithelium nyias nyias, thiab qib thiab cov kab mob ntawm cov kab mob tau txo qis (Daim duab 4A) . Txawm li cas los xij, kev kho mob nrog Cis zoo kawg nkaus txhim kho cov histology ntawm hypobaric hypoxia-induced testicular puas tsuaj hauv vivo (Daim duab 4A). Peb kuj ntsuas lub cev qhov hnyav, qhov hnyav, qhov hnyav ntawm lub cev, thiab qhov hnyav ntawm lub cev nqaij daim tawv, uas coj mus rau qhov ntsuas ntawm lub cev xeeb tub (lub cev xeeb tub / lub cev hnyav piv) raug xam. Raws li pom nyob rau hauv daim duab 4B-D, qhov ntsuas ntawm lub cev xeeb tub (testes, epididymis, thiab seminal vesicle gland) tau qis qis hauv pawg qauv (P < 0.01) dua li hauv pawg tswj hwm. Txawm li cas los xij, cov txiaj ntsig ntawm hypobaric hypoxia ntawm cov qog nqaij hlav hauv lub cev ntawm cov nas tau thim rov qab nrog Cis kev kho mob (Daim duab 4B-D).

Tom qab ntawd, cov kev ua haujlwm acrosome enzyme thiab cov phev nyob ntawm cov txiv neej nas phev kuj tau ntsuas los ua kom lub cev ua haujlwm puas tsuaj. Raws li pom nyob rau hauv daim duab 4E, 4F, acrosome enzyme kev ua si thiab phev motility tau qis dua nyob rau hauv cov qauv pab pawg nas tshaj nyob rau hauv pawg tswj (P < 0.01). Txawm li cas los xij, piv nrog cov nas hauv pawg qauv, acrosome enzyme kev ua haujlwm tau rov qab los hauv cov nas kho nrog 8 mg / kg / d Cis (P < 0.05) (Daim duab 4D). Ntxiv mus, raws li qhia hauv daim duab 4F, kev kho nrog Cis kujtxhim kho cov phev nyob; cov nas kho nrog 8 mg / kg / d Cis txhua tus pom tias muaj cov phev muaj sia nce ntxiv (55.83 ± 6.03%, P < 0.05; 69. {8}} ± 2.29%, P < 0.01; 52.33 ± 3.40%, P < 0.05; .

Ua ke, cov txiaj ntsig no tau qhia tias qhov chaw hypobaric hypoxic ua rau kev hloov pauv ntawm cov qog nqaij hlav morphological, kev ua me nyuam hauv nruab nrog cev poob, thiab kev ua haujlwm ntawm cov qog nqaij hlav hauv cov txiv neej nas, thiab Cis tuaj yeem tiv thaiv cov kab mob hauv plab los ntawm kev puas tsuaj los ntawm hypoxia.

Cov teebmeem ntawm Cis ntawm OS hauv cov qe ntshav ntawm cov nas hypobaric hypoxia-induced nas.

Cov qib ROS thiab LPO hauv cov qog ntawm cov nas tau ntsuas los ntsuas qhov cuam tshuam ntawm Cis ntawm hypobaric hypoxia-induced OS. Kev soj ntsuam ROS tau qhia tias piv rau pawg tswj hwm, qib ROS hauv cov qe ntshav hauv pawg qauv tau nce ntau (P < 0 01 Daim duab 5A). Hloov pauv, LPO tau nce siab hauv cov qog ntshav (P < 0.01) nyob rau hauv hypobaric hypoxia piv nrog cov mob normoxic (Daim duab 5B). Txawm li cas los xij, Cis kev kho mob hloov cov kev hloov saum toj no (P < 0.05), uas Cis-B tau txais txiaj ntsig zoo dua li lwm yam Cis (Daim duab 5A, 5B). Cis zoo li tiv thaiv cov qog los ntawm kev txo OS nyob rau hauv hypobaric hypoxic mob hauv vivo.

Tsis tas li ntawd, kev soj ntsuam apoptosis tau ua los soj ntsuam ntxiv cov txheej txheem uas Cis tiv thaiv hypobaric hypoxia-induced testicular function raug mob. Cov txiaj ntsig ntawm TUNEL staining (Daim duab 5C) tau pom tias muaj apoptosis tseem ceeb hauv pawg qauv piv rau pawg tswj hwm. Txawm li cas los xij, tom qab Cis (8 mg / kg / d) kev kho mob, tsawg dua apoptotic hlwb tshwm sim (P < 0.05) (Daim duab 5C). Cov ntaub ntawv Western blot kuj tau qhia tias kev kho mob hypoxia thiab hypobaric ua rau ua rau Caspase-3 thiab PARP thiab nce Bax / Bcl -2 piv rau hauv cov nqaij mos, qhia tias muaj kev nce hauv apoptosis (Daim duab 5D). Tsis tas li ntawd, ntau homKev kho mobtxo qis apoptosis hauv cov nqaij mos (Daim duab 5D). Ib yam li ntawd, kev soj ntsuam IHC ntawm cov qog nqaij hlav pom cov txiaj ntsig zoo sib xws (Cov duab ntxiv 1).

Txhawm rau txheeb xyuas cov txheej txheem ntawm Cis-txo OS tshwm sim los ntawm hypobaric hypoxia, peb tau sim ntxiv cov haujlwm ntawm GR, GPx, thiab SOD hauv cov nqaij mos. Raws li pom hauv daim duab 5E, piv nrog rau pawg tswj hwm, kev kho mob hypobaric hypoxia txo qis GR, GPx, thiab SOD cov dej num (P < 0.01). Txawm li cas los xij, Cis kev kho mob rov qab kho cov enzyme kev ua ub no (GR, GPx, thiab SOD) ntawm cov qog nqaij hlav hauv cov nas kho nrog hypobaric hypoxia (P <0.05). Hauv kev xaus, Cis zoo li tiv thaiv cov noob qes los ntawm kev ua kom muaj zog endogenous antioxidant enzyme tiv thaiv mechanism nyob rau hauv hypobaric hypoxia.

NATURAL CISTANCHE TUBULOSA rau kev txhim kho kev sib deev PHGS75% ECH 30% ACT 12%

Kev sib tham

Hauv thaj chaw siab siab, hypobaric hypoxia paub tias cuam tshuam rau ntau lub cev hauv tib neeg, suav nrog cov txiv neej kev ua me nyuam [4, 20]. Kev tshawb nrhiav tsis ntev los no yog npaj rau kev nkag siab txog cov txheej txheem ntawm yuav ua li cas hypobaric hypoxia impairs tus txiv neej txoj kev loj hlob. Nyob rau hauv txoj kev tshawb no, kev kho cov nyhuv ntawm Cis extract los ntawm Cistanches Herba ntawm hypoxia-induced reproductive puas raug soj ntsuam. Cov txiaj ntsig tau pom tias Cis tuaj yeem tiv thaiv tus txiv neej txoj kev ua me nyuam los ntawm kev puas tsuaj hypoxic los ntawm kev txo qis hypoxia-induced ROS tsub zuj zuj thiab OS los ntawm kev txhim kho cov kev ua ntawm endogenous antioxidant enzymes.

ROS yog oxygen-derived dawb radicals uas ua lub luag haujlwm tseem ceeb hauv tib neeg lub cev thiab kab mob. Kev noj tshuaj tsawg ntawm ROS yog qhov tseem ceeb rauphev capacitation, cov tshuaj tiv thaiv acrosome, thiab spermatozoa-oocyte fusion [24, 25]. Txawm li cas los xij, ntau dhau ntawm ROS feem ntau ua rau muaj kev puas tsuaj rau cov kab mob thiab cov hlwb stromal, ua rau txiv neej infertility [26]. ROS tuaj yeem ua kom puas cell membranes, nucleic acids, proteins, enzymes, thiab lwm yam macromolecules lom los ntawm peroxidation. Ntxiv mus, lawv kuj ua rau muaj peev xwm ntawm cellular thiab DNA puas thaum lawv tshaj lub peev xwm antioxidant-nqa. Cov ntaub ntawv pov thawj txhawb nqa lub luag haujlwm tseem ceeb ntawm ROS hauv pathogenesis ntawm txiv neej fertility [27, 28]. Kev tsim khoom ntawm ROS yog tswj los ntawm oxygen nro. Nyob rau hauv cov xwm txheej hypoxic, cov pa oxygen muaj nyob hauv ib puag ncig txo qis thiab cov ntshav viscosity nce, yog li cuam tshuam rau ntau cov txheej txheem oxygen-dependent metabolic hauv lub cev [29, 30]. Txawm li cas los xij, qhov qis atmospheric siab ntawm qhov siab siab ua rau tsis zoo venous rov qab thiab txo qis ntawm cov pa oxygen thauj los ntawm cov hlab ntsha mus rau tag nrho cov hlwb ntawm cov kab mob, uas ua rau cov hypoxia ntawm lub cev thiab cov hlwb ntxiv [29, 30]. Yog li, raug rau qhov siab siab ua rau muaj ntau cov lus teb ntawm hypoxic physiological, nrog rau kev tsim thiab tsub zuj zuj ntawm ROS, thaum cov pa oxygen xav tau ntau tshaj cov hlab ntsha. Raws li tau hais dhau los, kev sib sau ntawm ROS ua rau muaj ntau yam kev cuam tshuam hauv lub cev, qhov tseem ceeb tshaj plaws yog ua rau OS hauv hlwb.

OS hais txog qhov tsis sib xws ntawm oxidation thiab txo cov tshuaj tiv thaiv, ua rau lub cim ntawm oxidants ntau dhau los yog cov molecules uas txais ib qho hluav taws xob los ntawm lwm cov reactant, uas ua rau ROS [31, 32]. OS tau nkag siab zoo tias tuaj yeem tshwm sim los ntawm ntau yam endogenous thiab exogenous yam, suav nrog raug rau qhov siab siab. Spermatozoa yog cov hlwb uas tshwj xeeb tshaj yog rau OS muab lawv cov kev kho tsis zoo ntawm tes thiab cov ntshav plasma siab cov ntsiab lus ntawm polyunsaturated fatty acids [33]. Testicular thiab epididymal cov ntaub so ntswg yog tsis muaj kev zam, raws li lub xub ntiag ntawm hnyav OS tau pom nyob rau hauv ib ncig spermatids nyob rau hauv nas raug hypoxia [4]. OS cuam tshuam rau kev ruaj ntseg ntawm DNA, yog li ua rau muaj kev ncaj ncees ntawm gamete genetic khoom [34-36]. Txawm li cas los xij, qib siab ntawm DNA kev puas tsuaj hauv txiv neej gametes tau lees paub tias yuav ua rau muaj kev ua haujlwm ntawm apoptosis signaling, uas ua rau txo qis ntawm cov phev epididymal suav thiab nce feem pua ​​​​ntawm cov hlwb tsis zoo [28, 37]. Hauv txoj kev tshawb fawb tam sim no, hypoxia txo qis kev muaj peev xwm ntawm GC-1 hlwb los ntawm kev cuam tshuam ntawm apoptosis thiab cell voj voog raug ntes. Qhov tseem ceeb tshaj, qhov nce ROS ntau ntau tau pom los ntawm FCM kev tshuaj xyuas tom qab hypoxia stimulation, nrog nce apoptosis tus nqi thiab siab dua ua kom muaj zog ntawm Caspase-3, PARP, thiab Bax/Bcl-2 piv, qhia tias ROS tuaj yeem qhib tau. apoptosis los ntawm kev ua kom lub Caspase signaling txoj hauv kev thaum lub sij hawm hypoxia-induced fertility puas. Cov kev tshawb pom tam sim no pom tau tias hypoxia ua rau ntau dhau ROS tsub zuj zuj, ua rau oxidative puas tsuaj rau cov qe menyuam. Yog li, nws yog qhov muaj txiaj ntsig los txheeb xyuas cov tshuaj antioxidants tshiab uas tuaj yeem ua txoj hauv kev zoo los txo qis kev raug mob hypoxia vim yog fertility.

Txhawm rau tiv thaiv OS, cov kab mob antioxidant complex muaj nyob rau hauv lub cev, feem ntau yog tsim los ntawm enzymatic yam. Nyob rau hauv physiological tej yam kev mob, lub ROS ntsiab lus thiab antioxidant system tswj ib tug tej yam sib npaug. Txawm li cas los xij, ROS overproduction depletes cov phev antioxidant system, ua rau OS, uas ua rau phev DNA puas thiab ua rau qis fertility thiab cev xeeb tub cov nqi [23]. Yog li, txhawm rau hais txog ROS overproduction thiab cuam tshuam txog kev cuam tshuam ntawm cov cellular theem hauv cov txiv neej kev ua me nyuam, cov tswv yim sib txawv antioxidant tau raug sim [23]. Tam sim no, cov ntaub ntawv hais txog kev siv cov tebchaw nrog cov tshuaj antioxidant thiabtxhim kho cov phev ua haujlwmyog dav. Qhov tseem ceeb, feem ntau cov ntaub ntawv piav qhia txog kev txhim kho cov phev tsis tau tom qab noj cov tshuaj antioxidant hauv qhov ncauj, suav nrogtxhim kho cov phev concentrationthiab motility lossis txo qhov kev puas tsuaj DNA [38]. Yog li, ntau tus kws kho mob urologist tau muab tshuaj tua kab mob hauv qhov ncauj rau kev xeeb tub vim muaj teeb meem OS [39]. Cov antioxidants no suav nrog feem ntau carnitines, vitamins, zinc, melatonin, thiab natural compounds [23, 40]. Tam sim no, nrog rau kev txhim kho cov cuab yeej siv tshuaj rho tawm, ntau ntxiv ntawm TCM extracts kuj tseem raug txiav txim siab los txo cov txiv neej infertility vim tias cov tshuaj antioxidants tuaj yeem txo qhov kev puas tsuaj ntawm OS [41]. Yüce A. et al. tshaj tawm xyoo 2013 tias cinnamon muaj txiaj ntsig zoo ntawm oxidative thiab antioxidant tshuav nyiaj li cas hauv cov qe ntshav thiab phev zoo [42]. Zhang L et al. qhia tau hais tias curcumin ho txhim kho phev motility hauv cov neeg mob thiab txo H2O2 [43]. Tsis tas li ntawd, ntau yam ntawm lwm cov nroj tsuag rho tawm xws li blueberry, crocus sativus, pomegranate noob, thiab tshuaj yej ntsuab kuj tau pom tias yuav tiv thaiv kev ua me nyuam ntawm cov txheej txheem antioxidant [27, 44-47]. Cistanches Herba yog ib qho tseem ceeb TCM uas muaj qhov zoo tshaj plaws kev nyab xeeb profile thiab dav siv tshuaj kho mob rau kev xeeb tub, ntawm lwm yam mob [13]. Cov kev tshawb fawb tshuaj niaj hnub no tau pom tias Cistanches Herba muaj ntau yam dej num, xws li tshuaj tua kab mob, tshuaj tiv thaiv kab mob, hepatoprotective, thiab kev tiv thaiv kab mob hauv cov kab mob neurodegenerative [13, 48]. Yog li ntawd, extracts, feem, los yog cov tebchaw los ntawm Cistanches Herba tej zaum yuav muaj peev xwm antioxidant nta rau kev kho mob ntawm infertility.

Cov tshuaj nquag hauv cov nroj tsuag uas txhim kho fertility suav nrog ntau pawg tshuaj xws li PhGs, saponins, oxygenated volatile compounds, thiab alkaloids [41]. Pharmacological kev tshawb fawb ntawm PhGs tau pom tias PhGs nthuav tawm ntau yam bioactivities, xws li tshuaj tiv thaiv oxidation, antiradiation neuroprotection, thiab kev ua haujlwm ntawm kev sib deev [49, 50]. Ntawm cov dej num no, cov tshuaj tiv thaiv oxidation maj mam nyiam. Qee cov khoom siv ib leeg lossis cov feem ntawm PhGs tau tshaj tawm los tiv thaiv kab mob cell apoptosis tshwm sim los ntawm ntau yam tshuaj, thiab lawv cov peev xwm tiv thaiv oxidation hauv vitro kuj tau pom nyob rau hauv vivo hauv ntau tus tsiaj qauv [51, 52]. Cov txiaj ntsig no qhia tau tias PhGs tuaj yeem yog tus neeg sib tw txaus nyiam raukho txiv neej infertility. Cis yog ib qho PhG nquag uas tuaj yeem cais tawm ntawm Cistanches Herba. Hauv txoj kev tshawb fawb tam sim no, peb tau tshawb xyuas qhov cuam tshuam ntawm Cis ntawm cov kab mob hypoxia-kho lossis tus qauv nas thiab tshawb xyuas cov txheej txheem molecular hauv qab. Cis nthuav tawm cov haujlwm tiv thaiv ntawm kev txo qis hauv hypoxia-induced viability thiab nce hauv apoptosis hauv GC-1 hlwb, thiab nws kuj pom muaj kev tiv thaiv ntawm hypoxia-induced kev puas tsuaj nyob rau hauv lub cev xeeb tub ntawm nas nyob rau hauv vivo. Kev txo qis hauv GR, GPx, thiab SOD cov dej num hauv qab hypoxia hauv kev sib piv rau cov pab pawg normoxic tau pom, thaum cov haujlwm tshwj xeeb ntawm GR, GPx, thiab SOD nce ntxiv hauv cov qe ntshav lossis GC-1 hlwb kho nrog Cis. Cis zoo li tiv thaiv cov noob qes thiab GC-1 hlwb nyob rau hauv cov mob hypoxic los ntawm kev txhim kho cov haujlwm ntawm cov enzymes antioxidant.

Enzyme antioxidants ua haujlwm feem ntau los ntawm kev tshem tawm superoxide anions, yog li tiv thaiv lipid peroxidation thiab DNA puas tsuaj los tiv thaiv kev xeeb tub. Enzymatic antioxidant mechanisms ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv oxidative puas tsuaj [23]. Lub tshuab enzymatic tiv thaiv OS suav nrog dawb radical scavengers thiab glutathione-dependent enzymes suav nrog GR, GPx, thiab SOD [12]. Antioxidant enzymes tau nkag siab zoo tias yog qhov tseem ceeb rau txiv neej txoj kev loj hlob. Hauv txoj kev tshawb fawb tam sim no, cov txiaj ntsig ntawm kev txo qis antioxidant enzyme kev ua ub no nyob rau hauv hypobaric hypoxia yog nrog los ntawm kev nce ROS thiab LPO hauv pawg qauv, uas yog raws li cov ntaub ntawv dhau los [12]. Txawm li cas los xij, Cis kev tswj hwm tau coj mus rau kev rov qab los ntawm kev ua haujlwm antioxidant enzyme hauv GC-1 hlwb thiab cov tshuaj ntsuam nas, ua rau nws muaj peev xwm tsim cov tswv yim rau kev tswj hwm Cistanches Herba kom tsis txhob muaj kev puas tsuaj hypobaric hypoxia, raws li yav tas los hais. Txawm hais tias tam sim no cov txiaj ntsig tau pom tias kev kho mob nrog Cis ib nrab txo qis hypoxia-vim kab mob cell puas tsuaj hauv nas, kev tshawb nrhiav ntxiv yuav tsum tau nthuav tawm tag nrho cov duab ntawm nws cov kev tiv thaiv kev loj hlob. Piv txwv li, cov txheej txheem tshwj xeeb ntawm Cis cuam tshuam rau kev ua haujlwm ntawm cov enzymes antioxidant. Tsis tas li ntawd, muaj lus nug txog seb puas muaj lwm yam txheej txheem tseem tuaj yeem cuam tshuam raws li Cis tsuas yog qee qhov rov qab los ntawm kev ua me nyuam kev puas tsuaj los ntawm hypoxia. Thaum kawg, seb Cis puas muaj qhov cuam tshuam ncaj qha rau kev loj hlob ntawm cov kab mob yuav tsum tau txiav txim siab.

NATURAL CISTANCHE TUBULOSA RAU MAINTAINING SPERM ACTIVITY PHGS75% ECH 30% ACT 12%

Cov lus xaus

Feem ntau, qhov kev tshawb pom ntawm txoj kev tshawb no hais txog lub peev xwm ntawm Cis raws li ib qho antioxidant rau kev kho mob ntawm hypoxia-induced txiv neej kev loj hlob kev puas tsuaj. Cis tuaj yeem tiv thaiv hypoxia-induced txiv neej kev tsim kev puas tsuaj los ntawm kev rov ua haujlwm antioxidant enzyme, txo ROS-induced OS, ib txhij nce cell viability, thiab txo apoptosis. Qhov tseem ceeb, Cis subtypes (Cis-A, Cis-B, Cis-C, thiab Cis-H) tau kawm nyob rau hauv txoj kev tshawb no tag nrho tau pom muaj qee yam kev tiv thaiv ntawm lub cev xeeb tub, thiab Cis-B pom cov txiaj ntsig tseem ceeb tshaj plaws. Yog li ntawd, peb xav tias Cis tej zaum yuav yog ib tug zoo neeg sib tw antioxidant rau lubkev kho mob ntawm hypoxia-induced txiv neej reproductive puas, txawm hais tias qhov tseeb hauv qab mechanism yuav tsum tau tshawb xyuas ntxiv.