Cistanche Deserticola Extract nce pob txha tsim hauv Osteoblasts--Part I
Mar 15, 2022
Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791
Te Mao Liathiab a
Abstract
Lub Hom Phiaj Peb tshawb xyuas cov txiaj ntsig ntawmCistanchedeserticolaMa. (CD) raupob txhatsimlos ntawm kab lis kev cai osteoblasts. Cov txheej txheem Cov tshuaj mineralized nodule tsim tau siv los tshuaj xyuas cov teebmeem hauv vitro ntawm CD(Cistanchedeserticola)ntawmpob txhatsim. Alkaline phosphatase (ALP), pob txha morphogenetic proteins (BMP)-2, thiab osteopontin (OPN) mRNA qhia tau txheeb xyuas los ntawm ntau npaum li cas ntawm cov tshuaj tiv thaiv polymerase saw. Lub mechanism ntawm kev txiav txim ntawm CD(Cistanchedeserticola)Extract tau tshawb xyuas siv Western blotting. In-vivo anti-osteoporotic nyhuv ntawm CD(Cistanchedeserticola)extract tau soj ntsuam nyob rau hauv ovariectomized nas.
Cov kev tshawb pom tseem ceeb
CD(Cistanchedeserticola)extract tsis muaj kev cuam tshuam rau kev loj hlob, tsiv teb tsaws, lossis kho qhov txhab ntawm kab lis kev cai osteoblasts, tab sis nce ALP, BMP-2, thiab OPN mRNA thiab pob txha mineralization. Mitogen-activated protein kinase (MAPK) lossis nuclear factor (NF)-kB inhibitors txo CD(Cistanchedeserticola)extract-inducedpob txha tsimthiab ALP, BMP-2, thiab OPN qhia. Txawm li cas los xij, CD(Cistanchedeserticola)Extract tsis cuam tshuam rau osteoclastogenesis. Tsis tas li ntawd, CD(Cistanchedeserticola)extract tiv thaiv cov pob txha poob vim los ntawm ovariectomy hauv vivo.
Cov lus xaus
CD(Cistanchedeserticola)tej zaum yuav yog ib qho tshiabpob txha tsimtus neeg sawv cev rau kev kho mob osteoporosis.
Ntsiab luspob txha tsim; Cistanchedeserticola; Suav tshuaj ntsuab; osteoblasts
Cistanche deserticolayog qhov zoo raupob txha tsim
Taw qhia
Pob txha yog cov ntaub so ntswg uas tsim los ntawm ntau hom cell uas tau dhau los ua cov txheej txheem txuas ntxiv ntawm kev rov ua dua tshiab thiab kho lub npe hu ua 'pob txha kho'. Ob lub xovtooj loj ntawm lub luag haujlwm rau kev kho pob txha yog osteoclasts, uas resorb pob txha, thiab osteoblasts, uas tsim cov pob txha tshiab. Kev kho pob txha yog tswj hwm los ntawm ntau cov tshuaj hormones (xws li parathyroid hormone, 1, 25-dihydroxy vitamin D3, cov tshuaj hormones, thiab calcitonin) thiab cov khoom hauv zos (xws li nitric oxide, prostaglandins, kev loj hlob, thiab cytokines).[1] Osteoporosis tshwm sim thaum resorption thiab tsim cov pob txha yog uncoordinated thiab cov pob txha tawg ntau tshaj cov pob txha tsim.[2] Cov kev kho mob tam sim no rau osteoporosis muaj xws li bisphosphonates, calcitonin, thiab estrogen, uas yog cov pob txha resorption inhibitors uas tswj cov pob txha loj los ntawm inhibiting osteoclast kev ua ub no.[3] Txawm li cas los xij, cov nyhuv ntawm cov tshuaj no hauv kev nce lossis rov ua kom cov pob txha loj yog qhov me me, yeej tsis ntau tshaj 2 feem pua ntawm ib xyoos.[3] Yog li ntawd, muaj qhov xav tau rau cov pob txha tsim cov neeg ua haujlwm, xws li teriparatide, uas txhawb nqa cov tshiabpob txhatsimthiab kho qhov kev hloov pauv hauv trabecular microarchitecture uas yog tus yam ntxwv ntawm kev tsim pob txha pob txha.[4,5] Vim tias tshiabpob txhatsimfeem ntau yog ib qho kev ua haujlwm ntawm osteoblasts, cov neeg ua haujlwm uas ua los ntawm kev nce cov hlwb ntawm cov kab mob osteoblastic los yog ua kom muaj kev sib txawv ntawm cov osteoblasts tuaj yeem txhim kho pob txha tsim.[5,6]
Txawm hais tias cov txheej txheem ntawm osteoporosis tseem tsis paub meej, lawv yuav muaj feem cuam tshuam nrog kev txo qis lossis cuam tshuam ntawm cov pob txha loj hlob, xws li pob txha morphogenetic proteins (BMPs).[7] BMPs, uas muaj feem cuam tshuam nrog kev hloov pauv kev loj hlob zoo-b superfamily, yog thawj zaug txheeb xyuas los ntawm lawv lub peev xwm los ntxias ectopic.pob txha tsimhauv nas[8] BMPs ua lub luag haujlwm tseem ceeb hauvpob txhatsimthiab pob txha ntawm tes sib txawv los ntawm kev txhawb nqa alkaline phosphatase (ALP) kev ua haujlwm nrog rau kev sib txuas ntawm proteoglycan, collagen, thiab osteopontin (OPN).[9] Ib txoj kev tshawb fawb tsis ntev los no tau pom qhov sib txuas ntawm cov pob txha pob txha thiab cov polymorphisms tshwj xeeb hauv BMP-2, ALP, thiab OPN cov noob, qhia tias lawv muaj feem cuam tshuam nrog kev txhim kho pob txha pob txha.[10]
CistanchedeserticolaMa. (Orobanchaceae; abbreviated li CD)(Cistanchedeserticola)yog ib hom tshuaj ntsuab hauv Suav teb thiab tau siv dav hauv cov tshuaj ib txwm siv los ua tus neeg sawv cev kho mob vim nws cov tshuaj sedative, analgesic, thiab immunostimulatory zog.[11] Cov kev tshawb fawb pharmacological niaj hnub tau pom tias CD(Cistanchedeserticola)cov extracts tuaj yeem txhawb kev tiv thaiv kab mob, [12] tshem tawm cov dawb radicals[13], thiab ua kom muaj kev ua haujlwm ntawm superoxide dismutase.[14] Anti-inflammatory thiab antioxidant agents muaj peev xwm kho osteoporosis los ntawm kev ncepob txha tsimthiab/los yog suppressing pob txha resorption.[15,16] Txawm li cas los xij, cov nyhuv ntawm CD(Cistanchedeserticola)ntawm pob txha cell ua haujlwm tseem tsis tau txiav txim siab. Ntawm no, peb qhia tias CD(Cistanchedeserticola)extract tsis cuam tshuam rau kev loj hlob, tsiv teb tsaws, lossis kho qhov txhab ntawm kab lis kev cai osteoblasts, tab sis tau nce ALP, BMP-2, thiab OPN kev nthuav qhia thiab pob txha mineralization. Tsis tas li ntawd, peb qhia tau hais tias mitogen-activated protein kinase (MAPK) thiab nuclear factor (NF)-kB signaling pathways tej zaum yuav koom nrog hauv CD-mediated nce hauv cov noob qhia thiab pob txha mineralization. Hauv qhov sib piv, CD(Cistanchedeserticola)Extract tsis suppress osteoclastogenesis hauv vitro. Qhov tseem ceeb, kev kho cov nas nrog CD(Cistanchedeserticola)extract tiv thaiv pob txha poob vim los ntawm ovariectomy hauv vivo. Peb cov ntaub ntawv, yog li ntawd, qhia tias CD(Cistanchedeserticola)tuaj yeem siv los txhawbpob txhatsimrau kev kho mob osteoporosis.
Cistanche deserticolatxhawb kev tiv thaiv
Cov ntaub ntawv thiab cov txheej txheem
Cistanche deserticola extract thiab cov ntaub ntawv
CD(Cistanchedeserticola)Extract tau yuav los ntawm Chuang Song-Zong Pharmaceutical Company (Kaohsiung, Taiwan). Kev rho tawm thiab cais tawm ntawm CD(Cistanchedeserticola)tau ua raws li tau piav qhia yav dhau los (raws li cov ntaub ntawv spectral lawv tau txheeb xyuas cov tshuaj muaj pes tsawg leeg suav nrog beta-sitosterol, daucosterol, succinic acid, triacontanol, acteoside, betaine, thiab polysaccharide).[17] Luav polyclonal antibodies tshwj xeeb rau p-extracellular signal-regulated kinases (ERK), ERK, p-p38, p38, pc-Jun N-terminal kinases (JNK), JNK, p-p65, thiab p65 tau muas los ntawm Santa Cruz Biotechnology ( Santa Cruz, USA). Cov khoom siv osteocalcin ELISA tau yuav los ntawm Biosource Technology (Nivelles, Belgium). C-terminal telopeptides ntawm hom-I collagen ELISA cov khoom tau txais los ntawm Hla Laps (Herlev, Denmark). P38 dominant-negative mutant yog muab los ntawm Dr. J. Han (South-Western Medical Center, Dallas, USA). JNK dominant-negative mutant yog muab los ntawm Dr. M. Karin (University of California, San Diego, USA). Lub ERK2 tseem ceeb-tsis zoo mutant yog muab los ntawm Dr. M. Cobb (South-Western Medical Center). Tag nrho lwm cov reagents tau txais los ntawm Sigma-Aldrich (St Louis, USA).
Cell kab lis kev cai
Murine osteoblast cell kab MC3T3-E1 tau yuav los ntawm American Type Culture Collection (ATCC; Rockville, USA). Cells tau coj mus kuaj hauv 95 feem pua ntawm huab cua, 5 feem pua CO2 nrog a-MEM uas tau ntxiv nrog 20 hli HEPES thiab 10 feem pua ntawm cov fetal calf uas muaj cua sov, 2 hli-glutamine, penicillin (100 U / ml), thiab streptomycin (100 mg. /ml).
Kev ntsuas ntawm mineralized nodule tsim
Mineralized nodule tsim tau raug soj ntsuam raws li tau piav qhia.[18] Hauv luv luv, osteoblasts tau coj mus rau hauv qhov nruab nrab uas muaj vitamin C (50 mg / ml) thiab b-glycerophosphate (10 mm) rau ob lub lis piam, thiab qhov nruab nrab tau hloov txhua peb hnub. Tom qab incubation nrog CD(Cistanchedeserticola)extract rau 12 hnub, hlwb raug ntxuav ob zaug nrog 20 hli Tris-buffered saline uas muaj 0.15 m NaCl (pH 7.4), tsau rau hauv dej khov-txias 75 feem pua (v/v) ethanol rau 30 min, thiab cua- qhuav. Calcium deposition tau txiav txim siab siv alizarin liab-S staining. Luv luv, ethanol-taw hlwb thiab matrix tau stained rau 1 h nrog 40 mm alizarin liab-S (pH 4.2) thiab rinsed ntau nrog dej. Cov stain stain yog eluted nrog 10 feem pua (w/v) cetylpyridinium chloride thiab alizarin liab-S nyob rau hauv cov qauv raug ntsuas los ntawm kev ntsuas absorbance ntawm 550 nm thiab muab piv nrog tus qauv nkhaus. Ib lub mole ntawm alizarin liab-S xaiv khi kwv yees li ob moles ntawm calcium.
Cistanche deserticolayog qhov zoo raupob txha tsim
Quantitative real-time polymerase saw cov tshuaj tiv thaiv
Tag nrho RNA tau muab rho tawm los ntawm osteoblasts siv cov khoom siv TRIzol (MDBio Inc., Taipei, Taiwan). Kev hloov pauv rov qab tau ua tiav siv 2 mg tag nrho RNA thiab ib qho oligo(dT) primer.[19,20] Quantitative real-time polymerase chain reaction (qPCR) tau ua tiav siv TaqMan® ib-kauj ruam PCR Master Mix (Applied Biosystems, Carlsbad, USA). Tag nrho cDNA tau ntxiv (100 ng ib 25-ml cov tshuaj tiv thaiv) nrog cov txheej txheem tshwj xeeb primers thiab Taqman® sojntsuam. Tag nrho cov hom phiaj gene primers thiab sojntsuam tau yuav kev lag luam, suav nrog b-actin raws li kev tswj hwm sab hauv (Applied Biosystems). qPCR kev soj ntsuam tau ua tiav hauv triplicate ntawm StepOnePlus kab ke nrhiav kom tau qhov system (Applied Biosystems). Cov xwm txheej kev caij tsheb kauj vab yog 10-min polymerase activation ntawm 95 degree tom qab 40 lub voj voog ntawm 95 degree rau 15 s thiab 60 degree rau 60 s. Qhov chaw pib tau teeb tsa saum toj no tsis yog tus qauv tswj keeb kwm yav dhau los thiab nyob rau hauv theem kab ntawm lub hom phiaj gene amplification los xam cov lej ntawm lub voj voog uas tau txheeb xyuas cov ntawv sau tseg (tus cim CT).
Western blot tsom xam
Cell lysates tau npaj raws li tau piav qhia yav dhau los. Cov blots raug thaiv nrog 4 feem pua bovine serum albumin rau 1 h ntawm chav tsev kub thiab tom qab ntawd soj ntsuam nrog luav los tiv thaiv tib neeg cov tshuaj tiv thaiv p-65, los yog p-p65 (1: 1000) rau 1 h ntawm chav tsev kub. Tom qab peb ntxuav, cov blots tau incubated nrog peroxidase-conjugated nees luav los tiv thaiv luav theem nrab antibody (1: 1000) rau 1 h ntawm chav tsev kub. Cov blots tau pom los ntawm kev txhim kho chemiluminescence siv X-OMAT LS zaj duab xis (Eastman Kodak, Rochester, USA).
Ovariectomy-induced osteoporosis
Poj niam ICR nas, plaub lub lis piam, 22 ~ 28 g, tau siv rau txoj kev tshawb no. Cov nas tsuag tau ovariectomized ob sab nyob rau hauv trichloroacetaldehyde (100 mg / kg) tshuaj loog thiab cov nas tswj tau sham-operated (Sham) rau kev sib piv. Pob txha pob txha pob txha ceev thiab pob txha pob txha cov ntsiab lus tau ntsuas tom qab kev tswj hwm qhov ncauj ntawm ntau qhov ntau ntawm CD(Cistanchedeserticola)rho tawm txhua ob hnub rau plaub lub lis piam. Tag nrho lub cev pob txha ntxhia pob zeb ceev thiab cov pob txha pob txha cov ntsiab lus tau txiav txim siab los ntawm dual-zog X-ray absorptiometer (DEXA; XR-26; Norland, Fort Atkinson, USA) siv hom rau cov ncauj lus me raws li tau piav qhia yav dhau los.[18, 23] Tag nrho cov txheej txheem ua raws li cov txheej txheem hauv tsev kawm ntawv thiab tau txais kev pom zoo los ntawm Pawg Saib Xyuas Tsiaj ntawm Tuam Tshoj Medical University.
Kev txheeb cais
Kev txheeb cais tau ua tiav siv Prism 4.01software. (GraphPad Software Inc., San Diego, USA). Cov nqi muab yog txhais tau tias SEM. Kev txheeb xyuas ntawm ob qho piv txwv tau ua los ntawm Tub Ntxhais Kawm T-test. Kev sib piv ntawm ntau tshaj ob pawg tau ua los ntawm kev soj ntsuam ib-txoj kev ntawm kev sib txawv nrog Bonferroni qhov kev xeem posthoc. Hauv txhua kis, P <0.05 tau="" suav="" tias="" yog="" qhov="" tseem="">
Cistanche deserticolayog qhov zoo raupob txha tsim
Cov txiaj ntsig
Cistanche deserticolaextract nce pob txha mineralization los ntawm osteoblasts Ntxiv ntawm CD(Cistanchedeserticola)rho tawm rau 24 lossis 48 teev tsis cuam tshuam rau kev loj hlob ntawm nas osteoblast MC3T3-E1 hlwb hauv MTT kev soj ntsuam (cov ntaub ntawv tsis qhia). Vim tias kev sib txawv ntawm osteoblast yog cov txheej txheem nyuaj uas cuam tshuam nrog kev loj hlob ntawm tes thiab kev tsiv teb tsaws, peb kuj tau sim lub peev xwm tsiv teb tsaws ntawm MC3T3-E1 hlwb tom qab kho nrog CD.(Cistanchedeserticola)rho tawm. Siv Transwell thiab qhov txhab kho qhov txhab, peb pom tias CD(Cistanchedeserticola)extract tsis cuam tshuam rau osteoblast migration (cov ntaub ntawv tsis qhia). Kev tsim cov mineralized nodules yog ib qho ntawm cov cim ntawm osteoblast maturation. Alizarin liab S staining qhia tau hais tias mineralized nodules tsim thaum osteoblasts kab lis kev cai rau ob lub lis piam nyob rau hauv ib tug nruab nrab muaj vitamin C (50 ug / ml) thiab -glycerophosphate (10 hli), qhov concentration-dependent yam los ntawm kev sib ntxiv ntawm CD.(Cistanchedeserticola)(Daim duab 1a). Yog li ntawd, CD(Cistanchedeserticola)extract induced pob txha nodule tsim los ntawm kab lis kev cai osteoblasts, tab sis tsis yog lawv proliferation los yog tsiv teb tsaws. Peb kuj tau tshuaj xyuas lub luag haujlwm ntawm CD(Cistanchedeserticola)extract hauv RANKL-induced osteoclastogenesis tab sis pom tsis muaj txiaj ntsig (cov ntaub ntawv tsis qhia).
Cistanche deserticola extract nce alkaline phosphatase, pob txha morphogenetic protein-2, thiab osteopontin qhia hauv kab lis kev cai osteoblasts
Kev sib txawv ntawm cov osteoblasts nthuav qhia cov kev ua haujlwm siab ALP, uas cuam tshuam nrog cov qib siab ntawm cov enzymes nthuav qhia.[18] Peb pom tias kev kho mob nrog CD(Cistanchedeserticola)extract rau 72 h tau nce osteoblast ALP kev ua si (Daim duab 1b). Muab lub luag haujlwm tseem ceeb ntawm BMP-2, ALP, thiab OPN hauv kev sib txawv ntawm osteoblast, peb tau sim seb CD(Cistanchedeserticola)extract exerts nws cov nyhuv ntawm osteoblast txawv los ntawm kev tswj BMP-2, ALP, thiab OPN qhia. Kev kho cov hlwb nrog CD(Cistanchedeserticola)rho tawm ntau ntxiv mRNA qhia ntawm ALP, BMP-2, thiab OPN nyob rau hauv ib tug concentration-dependent yam (Daim duab 1c), qhia tau hais tias CD(Cistanchedeserticola)extract induced sib txawv ntawm osteoblasts los ntawm upregulating ALP, BMP -2, thiab OPN qhia.
Cistanche deserticola extract nce pob txha nodule tsim los ntawm mitogen-activated protein kinase txoj kev
Nws tau raug tshaj tawm tias MAPK plays lub luag haujlwm tseem ceeb hauvpob txhatsim[24,25] Yog li ntawd peb mam li tshuaj xyuas seb qhov kev taw qhia no puas koom nrog CD(Cistanchedeserticola)extract-induced pob txha mineralization. Kev kho cov hlwb nrog ERK inhibitor U0126, p38 inhibitor SB203580, lossis JNK inhibitor SP600125 txo CD(Cistanchedeserticola)extract-increased pob txha mineralization (Figures 2a, 3a, thiab 4a). Tsis tas li ntawd, cov inhibitors kuj thaiv CD(Cistanchedeserticola)extract-induced ALP, BMP-2, thiab OPN qhia (Figures 2b–4c). Kev hloov pauv ntawm cov hlwb nrog ERK2, p38, lossis JNK mutant txo CD(Cistanchedeserticola)extract-increase hauv ALP, BMP-2, thiab OPN mRNA qhia (Figures 2c, 3c, thiab 4c). Tom ntej no, peb ncaj qha tshuaj xyuas ERK, p38, thiab JNK ua kom tom qab CD(Cistanchedeserticola)extract kev kho mob. Incubation ntawm cov hlwb nrog CD(Cistanchedeserticola)extract induced ERK, p38, thiab JNK phosphorylation (Figures 2d, 3d, thiab 4d). Yog li ntawd, ERK, p38, thiab JNK kho qhov ncepob txhatsiminduced los ntawm CD(Cistanchedeserticola)kev kho mob ntawm osteoblasts.
Cistanche deserticola extract ua rau cov pob txha nodule tsim los ntawm txoj hauv kev nuclear-kB
Raws li tau hais dhau los, kev ua kom NF-kB yog qhov tsim nyog raupob txhatsim.[26,27] Peb tom ntej no kho osteoblasts nrog NF-kB inhibitors pyrrolidine dithiocarbamate (PDTC) thiab N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) tau yuav los ntawm Calbiochem (Darmstadt, Lub teb chaws Yelemees) los txiav txim seb NF-kB ua kom puas yog koom nrog CD(Cistanchedeserticola)extract-induced pob txha mineralization. Daim duab 5a qhia tau hais tias pretreatment ntawm osteoblasts nrog PDTC los yog TPCK inhibited CD(Cistanchedeserticola)extract-induced pob txha nodule tsim, thiab kuj txo qhov nce hauv ALP, BMP-2, thiab OPN qhia (Daim duab 5b thiab 5c). Phosphorylation ntawm NF-kB p65 subunit yog qhov qhia txog kev ua kom NF-kB; [28] raws li qhov no, peb pom tias CD(Cistanchedeserticola)extract nce p65 phosphorylation hauv osteoblasts (Daim duab 5d). Cov txiaj ntsig no tau qhia tias kev ua kom NF-kB yog qhov tseem ceeb rau CD extract-induced ALP, BMP-2, thiab OPN kev qhia thiab cov pob txha nodule tsim.
Daim duab 4 Kev koom tes ntawm c-Jun N-terminal kinases (JNK) hauvCistanchedeserticola(CD) extract-induced pob txha mineralization hauv osteoblasts. (a) Cells raug plated nyob rau hauv 24- zoo daim hlau thiab kab lis kev cai nyob rau hauv ib tug nruab nrab muaj vitamin C (50 mg / ml) thiab b-glycerophosphate (10 mM) rau ob lub lis piam. Cov hlwb raug kho nrog CD(Cistanchedeserticola)Extract (26 mg / ml) ntxiv rau SP600125 (3 mM). Cov mineralized nodule tsim tau raug soj ntsuam los ntawm alizarin liab-S staining (n=5). (b, c) Cells raug pretreated nrog SP600125 rau 30 min los yog hloov nrog JNK mutant los yog 24 h tom qab stimulation nrog CD extract, thiab alkaline phosphatase (ALP) kev ua thiab ALP, pob txha morphogenetic protein (BMP) -2 thiab osteopontin (OPN) mRNA qhia tau ntsuas los ntawm ALP cov khoom siv thiab ntau npaum li cas tiag-lub sij hawm polymerase saw cov tshuaj tiv thaiv. (d) Cells raug kho nrog CD extract rau lub sij hawm luv luv, thiab p-JNK qhia tau tshuaj xyuas los ntawm Western blotting. *P< 0.05,="" compared="" with="" control="" group;="">< 0.05,="" compared="" with="" cd="">(Cistanchedeserticola)extract-kho pab pawg.
Inhibition ntawm pob txha poob los ntawm Cistanche deserticola extract nyob rau hauv ovariectomized nas
Txhawm rau tshuaj xyuas cov nyhuv ntawm CD(Cistanchedeserticola)extract ntawm pob txha poob, osteoporosis yog induced nyob rau hauv poj niam nas los ntawm ovariectomy. Raws li qhov xav tau, ovariectomized nas pom tau txo qis tag nrho lub cev pob txha pob txha pob txha thiab pob txha pob txha cov ntsiab lus (Daim duab 6a thiab 6b). Kev kho mob nrog CD(Cistanchedeserticola)extract rau plaub lub lis piam inhibited qhov poob ntawm pob txha pob txha pob txha pob txha thiab pob txha pob txha cov ntsiab lus nyob rau hauv ib tug npaum li cas ntawm koob tshuaj (Daim duab 6a thiab 6b). Cov ntshav siab ntawm ALP tuaj yeem cuam tshuam txog kev ua haujlwm osteoblastic.[29] Raws li pom hauv daim duab 6c, CD(Cistanchedeserticola)Extract inhibited qhov txo qis hauv cov ntshav ALP kev ua haujlwm los ntawm ovariectomy. Tsis tas li ntawd, CD(Cistanchedeserticola)extract kuj nce qib ntawm osteocalcin, ib tug cim ntawmpob txhatsim, thiab txo cov qib C-terminal telopeptides ntawm hom-I collagen, ib qho cim ntawm pob txha resorption (Daim duab 6d thiab 6e). Cov kev tshawb pom no yog li txhawb nqa lub zog thiab kev ua tau zoo ntawm CD(Cistanchedeserticola)extract hauv kev tiv thaiv cov pob txha poob hauv vivo.
Daim duab 6 Inhibition ntawm ovariectomy-vim kom txo cov pob txha pob txha pob txha thiab pob txha pob txha cov ntsiab lus los ntawmCistanchedeserticola(CD) rho tawm. Poj niam ICR nas tau txais kev ua haujlwm sham los yog ovariectomized. Ovariectomized nas tau kho nrog qhov qhia tau tias muaj ntau ntawm CD(Cistanchedeserticola)extract los ntawm qhov ncauj noj. Tag nrho lub cev pob txha ntxhia qhov ntom ntom (a), cov pob txha ntxhia hauv cov ntsiab lus (b), ntshav alkaline phosphatase (ALP) kev ua haujlwm (c), ntshav osteocalcin (d), thiab ntshav C-terminal telopeptides ntawm hom I collagen (e), tau txiav txim plaub lub lis piam tom qab kev phais. Cov ntaub ntawv raug nthuav tawm raws li txhais tau tias SE, n=8–10 nas/pab pawg. *P < 0.05,="" piv="" nrog="" sham;="" #p=""><0.05, piv="" nrog="" ovariectomized="">
Kev sib tham
Los ntawm: 'Cistanchedeserticolaextract ncepob txhatsimnyob rau hauv osteoblasts 'los ntawmTe Mao Liaua al
---© 2012 Tus Sau. JPP © 2012 Royal Pharmaceutical Society 2012Phau ntawv Journal of Pharmacy thiab Pharmacology, 64Ib., 897–907
