Kev ua txuj ci mechanism thiab kev pom zoo ntawm cistancho verticanca hauv kev kho mob ntawm cov hnyuv qoj ⅱ
Nov 15, 2024
| Compound npe (Suav) | Tcmsp id | Molecular hnyav | Qhov ncauj Bioavailability |
|---|---|---|---|
| -Saib pestriroterol (植物甾醇) | Mol000358 | 36.91 | 0.75 |
| NCAUJ ROOJ (安诺炼) | Mol005320 | 45.57 | 0.20 |
| Xws li casillinolol (苏沟酚) | Mol005384 | 57.52 | 0.56 |
| yangambul (杨安补) | Mol007563 | 57.53 | 0.81 |
| Qerercitrin (槲皮素) | Mol000098 | 46.43 | 0.28 |
| myricetin (桑椹素) | Mol001454 | 37.05 | 0.68 |
| acetosid (毛蕨花酷) | Mol003333 | 2.94 | 0.62 |
| Ecodistsoid (脱皮激素) | Mol003734 | 3.14 | 0.38 |
| poliumoside (金合欢苷) | Na | Na | Na |
| Cistanoside A (肥皂树) | Mol008856 | 3.62 | 0.36 |
| Cistanoside B (肥皂树 b) | Mol008872 | 4.27 | 0.35 |
| Cistanoside C (肥皂树 c) | Na | Na | Na |
| Cistanoside D (肥皂树 d) | Mol00882 | 5.19 | 0.69 |
| Tubuloside A (管花草) | Na | Na | Na |
| Tubuloside B (管花草 B) | Na | Na | Na |
| isoacreosid (异亮芨芨草) | Mol107795 | 1.52 | 0.66 |
| 2- acetylacteoside (乙酰柠檬晶) | Na | Na | Na |
| L-arabinose (l- 阿拉伯糖) | Mol010200 | 54.12 | 0.03 |
| Galactosid (半乳糖) | Mol010203 | 10.22 | 0.55 |
| dulciol (半乳醇) | Na | Na | Na |
| D-glucaric acid (d- 葡萄糖酸) | Mol0129557 | 60.80 | 0.06 |
| mannitol (甘露醇) | Mol000003 | 17.73 | 0.03 |
| fructose (果糖) | Mol000053 | 1.68 | 0.03 |
| Xylose (木糖) | Mol000731 | 58.74 | 0.03 |
| piam thaj (葡萄糖) | Mol000734 | 24.44 | 0.03 |
| Rhaamnose (鼠李糖) | Mol004690 | 40.73 | 0.03 |
| sucrose (蔗糖) | Mol000842 | 7.17 | 0.23 |
| Mannose (甘露糖) | Mol000951 | 1.76 | 0.03 |
Ntawm no yog lub rooj txhais ua lus Askiv:
| Compound npe (Suav) | Tcmsp id | Molecular hnyav | Qhov ncauj Bioavailability |
|---|---|---|---|
| fructose (果糖) | Mol000053 | 1.68 | 0.03 |
| Xylose (木糖) | Mol000731 | 58.74 | 0.03 |
| piam thaj (葡萄糖) | Mol000734 | 24.44 | 0.03 |
| Rhaamnose (鼠李糖) | Mol004690 | 40.73 | 0.03 |
| sucrose (蔗糖) | Mol000842 | 7.17 | 0.23 |
| Mannose (甘露糖) | Mol000951 | 1.76 | 0.03 |
| Cistanin (肉桂素) | Mol008855 | 71.78 | 0.06 |
| Cistachlorin (肉桂苷素) | Na | Na | Na |
| Kankanosid A (甘菊苷) | Na | Na | Na |
| Kankanosid B (甘菊苷 b) | Na | Na | Na |
| {{0 {0}} epiloganic acid (8- 表乙酸) | Mol003203 | 98.51 | 0.09 |
| glucoside (榈苷) | Na | Na | Na |
| LiriDendrin (鹤掌枫脂素) | Mol007927 | 5.19 | 0.29 |
| Citrusin A (柑橘素) | Mol013438 | 25.04 | 0.73 |
| palripic acid (棕榈酸) | Mol000069 | 19.30 | 0.10 |
| niacin (烟酸) | Mol000421 | 47.65 | 0.02 |
Daim duab 1 "Cov Tshuaj Yeeb Tshuaj-Tshuaj-Hom Phiaj-Kab Mob" Sib Tham Network
3.1.4 Mus thiab KeGg Enrichment tsom xam
Raws li muaj nyob hauv cov duab 3 thiab 4, cov txheej txheem roj ntsha ntawm Cistanche Cistanche Condicorola hauv kev kho ib qho kev ua kom muaj protein itpings, thiab lwm yam.
3.1.5 molecular docking pov thawj
Molecular docking cov txiaj ntsig tau (rooj 2) qhia tau tias cov khoom siv khi ntawm cov tub ntxhais ntxhais lub hom phiaj thiab cov lus qhia tseem ceeb ntawm {1}}. 7 K}}. 7 Ntawm lawv, txoj hlua khi hluav taws xob ntawm AKT1 thiab -SiSterterol qhov zoo tshaj plaws kev sib xyaw.
Tshuaj ntsuab tshiab cistanche qhov ncauj ntxiv rau cem quav
Cov kev pabcuam txhawb nqa ntawm wecistanche-tus tshiab cistanche exporter nyob rau hauv Suav teb:
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3.2 Cov Ntsiab Lus Tsiaj Deev
3.2.1 Cov nyhuv ntawm CE nyob rau nas lub cev hnyav
Piv nrog rau cov pab pawg tsis sib haum, lub cev hnyav ntawm nas hauv pawg pawg tau raug txo (p <{}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}.} 1); Piv nrog rau cov qauv kev ua qauv, lub cev hnyav ntawm cov nas hauv pab pawg kev tswj hwm zoo thiab cov pab pawg ib pawg tau nce ntxiv (p <0.01). Cov txiaj ntsig tau tshwm sim hauv Table 3.
Daim duab 4 Kgg Txoj Kev Ntsuam Xyuas ntawm cov hom phiaj ntawm cistanche Showicola hauv kev kho ntawm IBD
Rooj 2 Docking Cov Ntsiab Lus Ntawm Cov Cheebtsam Cov Qauv thiab Cov Hom Phiaj Cov Proteins (KJ / Mol)
| Sib xyaw | TNF | Ua | Neeg tshuav | EGFr | SRc |
|---|---|---|---|---|---|
| Oleanolic Acid | -6.2 | -10.0 | -7.9 | -7.6 | -8.6 |
| Ursolic acid | -5.2 | -10.0 | -8.0 | -7.7 | -8.0 |
| Glycyrhetinic acid | -6.4 | -11.3 | -8.0 | -9.2 | -8.3 |
| {{0 {}} glycyrhetinic acid | -4.8 | -6.8 | -5.2 | -4.8 | -5.5 |
Rooj 3 lub cev hnyav, Tai, Txoj Kev Ntev Ntev, thiab Pamological tau ntawm cov nas hauv txhua pawg (x ± s)
| Pab | n | Hnyav (g) | Lub cev ntev (cm) | Dai (%) | Pathological tau |
|---|---|---|---|---|---|
| Kev tswj hwm | 8 | 20.84 ± 0.61 | 7.48 ± 0.18 | 0 | 0.13 ± 0.22 |
| Tus qauv | 7 | 14.24 ± 0.92a | 5.77 ± 0.35* | 3.30 ± 0.50* | 2.85 ± 0.61* |
| Tsawg Koob | 7 | 17.59 ± 0.75b | 7.47 ± 0.70b | 1.99 ± 0.45b | 0.93 ± 0.36b |
| Ce tsawg koob tshuaj | 7 | 16.23 ± 0.99b | 7.27 ± 0.42b | 2.66 ± 0.48b | 2.22 ± 0.60b |
| Ce Nruab Nrab Koob | 8 | 17.07 ± 0.67b | 7.37 ± 0.34b | 2.06 ± 0.31b | 1.51 ± 0.29b |
| Ce muaj koob tshuaj siab | 8 | 17.37 ± 0.41b | 7.43 ± 0.62b | 1.85 ± 0.43b | 1.13 ± 0.77b |
A: piv rau cov pab pawg tswj, p<0.01; b: Compared to the model group, P<0.01; c: Compared to the model group, P<0.05.
3.2.2 Cov nyhuv ntawm CE nyob rau nas Dai thiab Nyuv Ntev
Piv nrog pab pawg tswjfwm dawb, cov nas ntawm nas hauv pawg ua qauv tau nce, thiab txoj hnyuv ntev tau ua kom luv (p<0.01); compared with the model group, the DAI of rats in the positive control group and CE groups at each dose was significantly reduced, and the colon length was significantly shortened (P<0.01). All increased significantly (P＜0.05 or P＜0.01). The results are shown in Table 3 and Figure 5.
3.2.3 Qhov cuam tshuam ntawm CE ntawm keeb kwm athopathological morphology ntawm nas hnyuv hnyuv
Pab pawg nyo ntawm cov nas nyob rau hauv pab pawg tswjfwm tsis tau ua tiav thiab meej nyob rau hauv cov qauv, thiab tsis muaj mob cell infiltration thiab tus mob txhab tau pom; Cov nyo hauv nas nyob rau hauv tus qauv pab pawg ua tsis tiav, feem ntau ntawm cov gobblet hlwb ploj, thiab cov qauv crypt tau raug rhuav tshem; Piv nrog cov qauv sib piv cov pab pawg, cov hnyuv puas tsuaj ntawm cov nas nyob rau hauv cov kev tswj hwm ntawm cov hnyuv tau raug txo qis, thiab cov qhab nees<0.01). The results are shown in Figure 6 and Table 3.
3.2.4 Cov nyhuv ntawm CE ntawm cov qib ntawm cov khoom siv nyiaj hauv nas
Piv nrog pab pawg tswjfwm dawb, cov theem ntawm I6 {{}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} ntawm il -10 tau poob qis. (P <0.01); piv nrog cov qauv ua qauv, cov qib ntawm I -}}}}}}}}}}
Qib tau nce siab (p <{0. 01). Cov txiaj ntsig tau tshwm sim hauv rooj 4.
3.2.5 Cov qib ntawm cov protein tseem ceeb hauv nas cov ntaub qhwv
Piv nrog pab pawg tswjfwm dawb, cov protein protein qhia txog PI3K, EGR, cov nqaij mos ntawm cov pab pawg tau nce ntxiv (p< 0.05 or P＜0.01); compared with the model group, the expression levels of the above proteins in the colon tissue of mice in the positive control group and CE groups were significantly reduced (P＜0.05 or P＜0.01).
Cov txiaj ntsig tau tshwm sim hauv daim duab 7 thiab cov lus 5.
Rooj 4 kev sib piv ntawm cov theem ua kom zoo tshaj plaws hauv nas hnyuv sab hnyuv (x ± s)
| Pab | n | Il -10 (pg / ml) | Il -6 (pg / ml) | Il -1 (pg / ml) | TNF- (PG / ML) | MPO (U / L) |
|---|---|---|---|---|---|---|
| Kev tswj hwm | 8 | 47.0 ± 4.70 | 17.35 ± 0.64 | 291.27 ± 72.33 | 387.71 ± 116.76 | 23.06 ± 7.26 |
| Tus qauv | 7 | 3.68 ± 0.32a | 145.08 ± 23.86a | 338.94 ± 95.11a | 635.27 ± 135.15a | 97.06 ± 28.07a |
| Tsawg Koob | 7 | 44.74 ± 8.34b | 23.23 ± 4.64b | 300.52 ± 84.71b | 448.21 ± 123.28b | 38.60 ± 16.78b |
| Ce tsawg koob tshuaj | 7 | 12.87 ± 3.26b | 81.12 ± 18.95b | 331.24 ± 95.80b | 510.81 ± 110.63b | 51.61 ± 11.62b |
| Ce Nruab Nrab Koob | 8 | 21.80 ± 4.12b | 50.75 ± 22.16b | 323.62 ± 79.21b | 508.68 ± 146.97b | 45.53 ± 15.21b |
| Ce muaj koob tshuaj siab | 8 | 31.10 ± 7.40b | 27.55 ± 41.22b | 294.13 ± 86.05b | 461.42 ± 140.30b | 44.26 ± 13.58b |
A: piv rau cov pab pawg tswj, p<0.01; b: Compared to the model group, P<0.01.
A: Pawg tsis raug; B: MODEL GROUP; C: Cov pab pawg tswj tau zoo; D: CE cov pab pawg siab; E: CE cov pab pawg nruab nrab hauv nruab nrab; F: CE cov pab pawg tsawg-tshuaj.
Daim duab 7 Electrophoresis ntawm cov tub ntxhais protein hauv cov hnyuv ntawm cov nas hauv txhua pab pawg
4 kev sib tham
Hauv txoj kev tshawb fawb no, cov txiaj ntsig ntawm molecular docking kev sim qhia tias cov hom phiaj tseem ceeb Nws qhia txog cov yam ntxwv synergistic ntawm cistanche Squaticola cov khoom siv ntau thiab ntau hom.
Cov kev tshawb fawb tau qhia tias kev tawm tsam cov plaub hau tuaj yeem ua rau muaj kev zais cia thiab tawm ntawm cov kev cuam tshuam nrog thiab muaj kev tiv thaiv zoo [17]. Suzilide tuaj yeem txhawb nqa cov zais ntawm gamma-interferon los ntawm kev tiv thaiv kab mob, thiab muaj cov txiaj ntsig ntawm cov kev tiv thaiv kab mob ntawm cov qog ntshav. Suzilide thiab quercetin kuj muaj cov nyhuv ntawm kev txhawb nqa lub zais ntawm gamma-interferon los ntawm kev tiv thaiv kab mob. Synergy [18].
-Saib cov neeg muaj peev xwm daws tau tus mob colevoning los ntawm downregulating cov qib mob xws li}} {2}}, thiab tnf- [19]. Cissaneside h tuaj yeem tswj hwm oxidative kev nyuaj siab thiab cov lus teb rau kev pom zoo li glycerophosphapip metabolism thiab Aracidonic Acid metabolism [20].
Nws tuaj yeem pom tau tias cov plaub hau, suzilide, -susterol, thiab cistance glycoside h tej zaum yog cov khoom ntawm Cistanche Showicola hauv kev kho ib qho ntawm IBD.
KGG yuav nrhiav pom tias Cistanche Showicola ua rau muaj kev kho mob rau IBSULTECTOLA xws li PI3K / AKF Tyrosine Kinase inhibitor tsis kam. Cov kev tshawb fawb tau taw qhia tias kev ua haujlwm ntawm PI3K / AKT signaling txoj hauv kev tuaj yeem txhawb nqa kev puas tsuaj [21].
SRC yog qhov nce toj ntawm cov molecule ntawm pi3k / akt signaling txoj kev. DSS nkag mus hauv cov hlwb tuaj yeem qhib kev ua haujlwm ntawm lub cev uas tsis muaj zog ntxiv rau lub cev ua rau muaj kev cuam tshuam nrog lub cev ua rau lub cev tsis muaj zog [22].
Egfr tuaj yeem yog phosphorylated los ntawm kev khi los ntawm cov protein cuam tshuam, thiab tom qab ntawd kev sib koom tes nrog cov lus teb kev qhia txog kev kho cov lus teb ntsig txog kev kho mob [23]. AKT1 tuaj yeem ua kom muaj kev cuam tshuam Nuclear Nuclear κb, yog li txhawb cov synthesis thiab tso tawm cov profammatory thiab ua kom nrawm dua cov lus teb scampampatory [24].
Stat3 yog cov hom phiaj tseem ceeb protein ntawm Jak / Stat signaling txoj kev. Thaum Stat3 tau ua nrawm nrawm, nws tuaj yeem nrawm qhov kev tso tawm ntawm cov khoom siv hluav taws xob xws li}}}}}, ua rau kom nce ntxiv rau hauv inflammatory infiltration [25]
Cov. TNF- yog thawj cytokine tso tawm hauv kev tiv thaiv kab xeb, tuaj yeem sib kho plab hnyuv viav vias los ntawm cov kev sib txawv ntawm cov kev sib txawv ntawm tes epithelial cell agoptosis [26].
Nws tuaj yeem pom tau tias TNF-, AKT1, AID3, EGFR, thiab SRC kuj yog cov hom phiaj tseem ceeb ntawm Cistanche Squaticola hauv kev kho IBD. Cov kev sim tsiaj ntxiv pom tias CE tuaj yeem txo cov protein tshaj tawm ntawm cov protein saud, ua pov thawj ntawm cov chaw muag tshuaj porticals.
Hauv cov ntsiab lus, qhov kev kawm no tau lees paub txog cov nyhuv ntawm cistanche Squaranceola hauv kev kho ibD ntawm IbD los ntawm Network Pharpacology thiab kev sim tsiaj. Nws cov txheej txheem tshwj xeeb ntawm kev nqis tes ua yuav cuam tshuam nrog cov kev cai ntawm SRC / EGF / PI3K / AKT signaling txoj kev.
