Cistanche Squaticola Polysaccharides daje Amergety Chenuelety hauv kev cuam tshuam rau lub plab muaj zog 4 / nuclear Peretor 4 / Nuclear Performoring Txoj Kev-κB
Nov 05, 2024
Abstract: Lub hom phiaj ntawm txoj kev tshawb fawb no tau tshawb xyuas cov nyhuv ameliorive thiabCov peev txheej ntawm cistanche Showicola Polysaccharides (CDPS)saumDiabety Mephopathy (DN)hauv nas. DN cov tub ntxhais tus qauv DN tau tsim los ntawm kev ua kom muaj rog rog (HFD) pub ua ke nrog kev txhaj tshuaj ntawm streptoozotocin (STZ). Peb caug-rau C57BL / 6N txiv neej nas tau faib ua pawg (n=6 txhua). Txhua pawg tau raug tswj hwm nrog dej distilled (tswj hwm thiab qauv), dapagliflozin lossis CDPS rau 4 asthiv. Kev hloov pauv hauv lub cev qhov ntsuas hluav taws xob, lub raum muaj nuj nqi,Inflammatory yam ntxwvthiab lub pob zeb hnyuv laus tau raug ntsuas. Cov txiaj ntsig tau pom tias cdps loj dua txhim kho qhov kev mob thiab muaj zogLub raum histopathological hloov, thiabTxo cov theem ntawm cov ntshav ntshav ntshav, itinary proteinthiabInflammatory yam ntxwvHauv cov nas. Ib qho ntxiv, CDPS cuam tshuam qhov sib npaug ntawm cov kab mob plab zom mov thiab cov kab mob muaj txiaj ntsig zoo, uas nyob hauv qhov tig txhim kho txoj hnyuv. CDPS kuj tseem tso cai ntawm tus xov tooj-nyiam receptor 4 / nuclear factor-κb (tlr4 / nf-κb) signing txoj kev lipopolysacysacysacysacysacysacylide (LPS). Hauv kev xaus, CDPS nthuav tawm cov nyhuv ameliorive rau DN hauv kev cuam tshuam los ntawm kev cuam tshuam lub plab hnyuv puab muv thiab inhibiting signing signaling pathways. Qhov kev tshawb pom no muaj cov kev pom tshiab rau hauv kev kho ntawm DN.
Cov Ntsiab Lus: Cistanche Showicola Polysaccharides;Diabetchy; txoj hnyuv hnyuv; Mob plab hnyuv. Hu-zoo li receptor 4; nuclear factor-κb
Cistanche Squaticola extract nrog cov khoom siv raw zoo
Nyem kom tau txais cov ntsiab lus ntxiv
Ntshav Qab Zib Mephopathy (DN)yog qhov ua rau muaj kab mob raum nyob kawg thiab mob siab rau lub neej thiab kev noj qab haus huv ntawm cov neeg mob [1]. Cov kev tshawb fawb tau lees tias txoj hnyuv Flora tsis zoo ua ib qho txheej txheem tseem ceeb hauv kev txhim kho hauv DN [{2}]. Lub "lub plab-axis" Txoj kev xav ntseeg tias kev hloov pauv ntawm lub plab zom mov ntawm lub plab zom mov ntawm cov hnyuv roj av uas muaj kev tswj hwm ntawm plab hnyuv muaj zog. Cov plab hnyuv muaj ua lub luag haujlwm tseem ceeb hauv kev tswj hwm lub raum muaj nyob hauv DN nas models [{5 5}}]. Qhov kev tshawb fawb hauv "plab-axis" yuav pab qhia meej txog pathogenesis ntawm DN Pathogenesis ntawm DN thiab nrhiav tau cov hom phiaj tshiab rau kev tiv thaiv thiab kho.
Cistanche Squaticola yog ib txwm tshuaj tshuaj thiab khoom siv tau, tsis muaj npe, Jinsun, thiab lwm yam ntawm "Suab puam Ginseng" [6]. Raws li cov neeg muag tshuaj ntawm cov neeg koom pheej ntawm Tuam Tshoj, cistanche Showicola yog qhov qhuav formesicola yc ma lossis c. Yakhauj
Ntawm lawv, cistanche Squaticola muaj keeb kwm ntev los siv tshuaj thiab khoom noj. Cistanche Squaticola yog qab zib, qab ntsev, thiab sov nyob rau hauv qhov thiab sov thiab nkag mus rau lub raum thiab cov hnyuv roj meridians. Nws muaj qhov cuam tshuam ntawm lub toninging rabney yaj, tau txiaj ntsig cov ntshav, thiab noo noo illiping [7-8]. Polysaccharides yog ib qho ntawm cov khoom xyaw tseem ceeb ntawm cistancey Squaticola. Cov ntsiab lus ntawm polysaccharides hauv contanche Showicola dej extract yog qhov tseem ceeb. Nws tsuas yog tsim ntawm Monosaccharides xws li cov piam thaj, galactose, rhamnose, arabinose, thiab fructose. Nws muaj ntau yam kev ua kom muaj zog xws li kev siv tshuaj tiv thaiv kab mob, kev txhim kho kev ua haujlwm, tiv thaiv lub siab, tiv thaiv kab mob, thiab tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob, thiab cov tshuaj tiv thaiv kab mob tawm tsam,}}}]. Cov kev tshawb fawb dhau los los ntawm pab pawg tshawb fawb tau qhia tias Cistancho Desertico Dej Extract muaj cov peev xwm kho tau rau DN [13]. Raws li qhov no, qhov kev sim no tau coj cov plab muaj zog thiab kev hloov pauv ntawm lub raum ua haujlwm, txhawm rau muab lub hauv paus science rau lub luag haujlwm ntawm CDPS hauv kev kho DN.
1 cov ntaub ntawv thiab cov hau kev
1.1 Tsiaj, Khoom thiab Reagents
{{0 {0}}} Lub lim tiam C57BL / 6J tus txiv neej tau yuav los ntawm cov chaw kho mob tsiaj ntawm Xinjiang Medical University, Chaw Tsim Tshuaj Ntau.: Kev daws teeb meem tsis muaj.: Iacuc -20210331-04.
Suab puam CDPs (polysaccharide pawg feem ntau 80%, batch tooj: 2306019) Shaanxi Xintianyu tuam txhab; Dapagliflozin (Dapa; Batch Zauv: 2108119) AstraSeca Kws Tshuaj Co.: LTD.; Tso zis tag nrho cov protein (tso zis tag nrho cov protein, utp; creat} Nitrea Nitrogen, Creat}, {}} C 012-2-1) Cov Khoom Siv Nanjing Jiancheng Bioengineering Institute; Lipopolysaccharide (LPS; Batch Tus lej: Yx {}}} ({} ({} ({} (tgf-; transform); YX-E20217), Qum Necrosis Qhov Tshwj - (TNF-; Batch Tus Xov Tooj: Yx-E20220) Kit Shanghai Youxuan Biologi Waldan.; Ltd.; Enhanced chemiluminescene (ECL) Khoom Siv Beijing Pulilai Gene Technology Co., Ltd;; Streptoozotocin (STZ; Batch Zauv: 20230503, muaj roj ntau (cov khoom noj muaj roj: Boaiigang12492m) Beijing Boaiigang Biotechnology Co., Ltd.
Cov khoom noj yooj yim (muaj 22% hmoov nplej, 12% cov roj uas muaj nyiaj,} {}}}. 25%}. 25%}. 25%}. 25%}. 25%} Xinjiang Medical University.
1.2 cov twj siv thiab cov khoom siv
AE240 ntsuas kev sib npaug, mettler-toledo ntsuas Co., Ltd., Switzerland; DHG -9053 Lub tshuab hluav taws xob hluav taws xob tawg tawg qhuav cub, shanghai yiheng scientific Ntsuas Co., Ltd.; DK-S22 tas li dej da dej da dej, shanghai jinghong simtal ntsuas Co., Ltd.; {{5 5}} ks high-ceev tsawg-kub centrifuge, sigmaaldrich, USA; Bs -380 tag nrho cov neeg siv hluav taws xob tag nrho tsis siv neeg EM KMR3 Slicer, Hi1220 Slicer, Leitz Opten Lub Vev Xaib, Leica, Lub Tebchaws Yelemees; Fluorchem e Chemiluminescence Yees system, Proteinsimple, USA.
1.3 cov hau kev
1.3.1 tus tsiaj sim
36 C57BL / 6J Tus txiv neej nas tau pub nyob rau hauv SPO chaw kuaj xyuas kev kho mob thiab pub dawb. Qhov kev sim tau nqa tawm tom qab 7 hnub ntawm kev pub mis. Rau tus nas tau xaiv los ua ib txwm (tswj) pawg thiab muab cov zaub mov yooj yim. Tus so ntawm cov pab pawg kws sim tau noj cov zaub mov muaj rog ntau thiab khaws cia hauv nyias cov tawb. Lub cev hnyav tau sim ib zaug ib lub lim tiam. Cov nas tsuag tau noj mov dawb thiab haus dej. Tom qab 6 lub lis piam ntawm kev noj haus muaj rog muaj rog, cov pab pawg sim ua ke rau cov zaub mov muaj roj ntau txhua hnub. Kev yoo mov ntshav qab zib tom qab ua qauv yog 11.1 MMOL / L, uas tau txiav txim siab yog tus qauv ua kom muaj ntshav qab zib. Peb caug nas nrog kev ua tiav tau faib ua tsib pawg, Dapa) Pab Pawg, CDP / KG / KG / KG / KG / KG / KG / KG / KG + Kev Noj Qab Haus Huv (CG / KG Cdps-m) pawg, thiab CDPS cov tshuaj muaj zog (200 mg / kg - roj noj kom zoo, cdps-h) pawg. Cov pab pawg tswj hwm (distilled dej + kev noj haus ib txwm muaj) tau muab ib qho sib npaug ntawm cov dej distilled. Txhua tus nas yog gavaged ntawm 0.1 ml / 10 g, ib zaug ib hnub nyob rau tib lub sijhawm, rau tag nrho 4 lub lis piam. Lub cev hnyav thiab ntshav ntshav zoo li cas ntawm cov nas tau sau cia txhua lub lim tiam.
1.3.2 Kev Txiav Txim Siab
Tom qab 4 lub lis piam ntawm kev coj ua guar Cov nas tsuag tau yoo mov tab sis tsis yog watered rau 12 h. Hnub tom qab, lub cev nrawm ntawm cov nas tau ntsuas. Cov nas tsuag tau loog los ntawm kev txhaj tshuaj ntawm kev tsim cua ntawm 3 ml / kg ntawm 10% hydroral hydrate daws. Cov ntshav tau sau los ntawm cov orbits thiab centrifuged nyob rau hauv ib qho centrifuge (4 degree, {9 {9} r / min) rau 15 min. Tus supernatant tau ASPIRATED, ntim khoom, thiab khaws cia rau hauv {{11 {} degree tub yees rau kev siv tom qab. Cov ntshav dej yog ntsuas rau bun, crre, ua,} {}}}}} {}, tnf-, thiab lps raws li cov khoom siv. Cov zis tau ntsuas rau 24 h utp raws li cov khoom siv.
1.3.3 morphological thiab kev soj ntsuam ntawm lub raum thiab txoj hnyuv
1.3.3.1 Kev txiav txim siab ntawm lub raum coefficient
Cov kev sau ua ntshav, cov nas tau ua kom tuag los ntawm kev tuag los ntawm kev tuag los ntawm kev tshem tawm thiab muab cov dej qab zib kiag li Lub raum coefficient tau muab xam raws li cov hauv qab no: Lub raum coefficient /% {1}}}} m yog lub cev hnyav ntawm nas / g.
1.3.3.2 Kev Sumbological ntawm lub raum
Cov nas tsuag nas tau tsau nyob rau hauv 4% pararormaldehyde kho, thiab tom qab ntawd siv los ntawm ethanol daws, thiab eosin (H & E), thiab ib txwm muaj acid-schiff (Pas), kaw, thiab cov duab tau sau thiab pom hauv qab tsom iav.
1.3.3.3 Txoj hnyuv soj ntsuam
Tus nas txoj hnyuv tau tsau nrog 4% pararormaldehyde txhim kho, pob tshab nrog kev tawm tsam, AB) -PAS rau kev sib khi, thiab cov duab tuab rau hauv qab lub tshuab tsom rau kev soj ntsuam.
1.3.4 Tsom xam ntawm txoj hnyuv uas muaj pes tsawg leeg
Cov hnyuv los ntawm cov nas hauv pawg tswj, pab pawg ua, thiab CDPS-H pab pawg tau kuaj pom los ntawm 16s RRNA Cov noob siab siab-throughping exctrencing. DNA rho tawm thiab kev tsim kho tsev qiv ntawv thiab ua ntu zus ntawm cov kab mob ntawm Lianchynology tau ua tiav los ntawm Lianchhanism Biotezism Co., Ltd., thiab cov ntaub ntawv tau txheeb xyuas ntawm Lianchuan huab platform.
1.3.5 Nrhiav kom muaj lub raum thiab cov proxais ntsig txog cov protein
Tus nas Rabney thiab cov hnyuv ntxig cov nqaij ntxig tau muab rho tawm, thiab cov protein tau kom muaj nuj nqis los ntawm cov protein ntau. Tom qab protein denaturation, cov protein tau khaws cia rau hauv -20} degree tub yees. 10% sodium dodecyl sulecyl sulecyl sulecyl sulecyl sulecyl sulecate-polyvrylamide, polyvane tau muab tso rau hauv ib qho tris buffered filine nrog kaum ob} (TBST) Tank, thiab thaiv ntawm chav tsev kub. Kev pom
Cov tshuaj tiv thaiv tseem ceeb rau hu-nyiam cov receptors (nf-} ({}} occ{ulaody} (Zo {{{{{}}) hauv cov hnyuv (tshuaj tiv thaiv diluted 1: {} (v / v) nrog tbst daws teeb meem thaum hmo ntuj. TBTSilution, tshuaj tiv thaiv theem nrab (antibody
diluted nrog tbst daws 1: 2 000 (v / v)), thiab tom qab ntawd nrhiav tau los ntawm kev ntxiv cov lus qhia txog kev ntxiv cov tshuaj tiv thaiv ecl -Txoj kev siv tau siv los ua cov khoom siv sab hauv, thiab cov protein grey tus nqi tau txheeb xyuas los ntawm Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Im Imsia Qhov protein qhia ntawm txhua pawg tau qhia raws li tus txheeb ze tus nqi ntawm cov nqi sib cav ntawm pawg tswj hwm.
1.4 Cov Ntawv Txheeb Xyuas Cov Ntaub Ntawv
Tag nrho cov ntaub ntawv cov txiaj ntsig tau qhia tias yog ± S, thiab UPSS 21. 0}}}} tau siv rau kev tsom xam. Rau cov ntaub ntawv txuas ntxiv, yog tias lawv tau ua raws li ib qho kev faib tawm ib txwm, ib txoj kev ntsuas ntawm kev sib txawv tau siv rau kev sib piv hauv pawg. Yog tias qhov sib txawv ntawm cov pab pawg tau yog cov ntsiab lus tseem ceeb, tus menyuam qaib ntxhw txoj kev tau siv ntxiv rau kev sib piv pairwise; Yog tias cov ntaub ntawv ua raws li ib qho kev faib tawm ib txwm, Kruskal-Wallis H sim siv rau kev sib piv hauv pawg. Thaum muaj ib qho txawv ntawm cov pab pawg, durbin tsawg kawg qhov qauv sib txawv tau siv ntxiv rau ntau qhov sib piv. P <{{6 {6}}. 05 qhia tias qhov txawv yog qhov tseem ceeb tshaj plaws.
2 cov qhab nia thiab tshuaj ntsuam
2.1 nyhuv ntawm CDPS ntawm cov ntshav ntshav qab zib, lub cev hnyav, thiab raum coefficient ntawm C57BL / 6J
Raws li tuaj yeem pom los ntawm cov rooj 1 txog 3, piv nrog cov pab pawg tswj hwm, kev yoo ntshav ntshav qab zib ntawm cov tsiaj muaj nce ntxiv (p<0.01), and the kidney coefficient was significantly increased (P<0.01); compared with the Model group, CDPs- The body weight of mice in group H increased significantly after 4 weeks of administration (P＜0.05), and blood sugar began to decrease after 3 weeks of administration (P＜0.05). The kidney coefficient of mice in each intervention group decreased, and the CDPs-H group was the lowest (P<0.01); the body weight of mice in the Control group was larger than that of each group, indicating that CDPs can slow down the swelling of the kidneys of DN mice, thereby Reduce the kidney coefficient.
Cov lus 1 Teeb meem ntawm CDPS ntawm cov ntshav qab zib ntawm cov nas (n {1}})
| Pab | Sijhawm / Lub Limtiam | ||||
|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | |
| Kev tswj hwm | 7.98±0.55 | 7.32±0.40 | 7.58±0.50 | 7.38±0.87 | 7.28±0.34 |
| Tus qauv | 10.62±2.79# | 20.32±2.69# | 24.47±4.18# | 24.92±3.98# | 20.33±2.96# |
| Cdps-l | 16.03±2.97 | 21.83±4.81 | 20.13±6.97 | 23.30±3.77 | 21.25±1.65 |
| Cdps-m | 13.17±3.59 | 22.6±5.88 | 19.35±4.45 | 22.6±4.87 | 18.38±1.20 |
| Cdps-h | 11.07±1.74 | 21.53±3.71 | 21.57±6.19 | 17.43±2.05** | 15.87±0.90** |
| Dapa | 10.28±4.75 | 21.12±4.96 | 17.72±2.00** | 17.35±2.00** | 14.55±1.06** |
Chav: MMOL / L
Cov lus 2 Qhov cuam tshuam ntawm CDPS ntawm lub raum qhia ntawm nas (n=6)
| Pab | Kev tswj hwm | Tus qauv | Cdps-l | Cdps-m | Cdps-h | Dapa |
|---|---|---|---|---|---|---|
| Raum / lub cev hnyav% | 0.98±0.04 | 1.69±0.17## | 1.42±0.12 | 1.37±0.10* | 1.28±0.10** | 1.18±0.10** |
Cov lus 3 teebmeem ntawm CDPS ntawm lub cev loj ntawm nas (n=6)
| Pab | Sijhawm / Lub Limtiam | ||||
|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | |
| Kev tswj hwm | 28.57±0.82 | 28.75±0.97 | 28.70±0.79 | 29.10±1.24 | 29.32±1.35 |
| Tus qauv | 24.53±1.25## | 24.92±1.20## | 24.35±1.39## | 24.82±1.22## | 24.73±1.56## |
| Cdps-l | 25.75±0.69 | 24.77±0.91 | 24.83±0.99 | 25.10±0.83 | 25.42±0.97 |
| Cdps-m | 25.10±0.56 | 25.00±0.54 | 24.68±0.48 | 25.13±0.62 | 25.25±0.58 |
| Cdps-h | 25.38±0.28 | 25.43±0.49 | 25.52±0.62 | 25.73±0.50 | 26.08±0.34* |
| Dapa | 25.13±1.02 | 25.52±0.98 | 25.97±1.00* | 26.25±0.63* | 26.63±0.78* |
2.2 Cov nyhuv ntawm CDPS ntawm lub raum muaj nuj nqi ntawm C57BL / 6J nas
Raws li qhia hauv Table 4, piv nrog pab pawg tswj, tus bre, thiab UTP kev pom zoo hauv cov tshuaj ntsuab hauv cov pab pawg tau nce ntxiv (p<0.01); compared with the Model group, CDPs-M and CDPs-H can significantly reduce the concentrations of BUN, Cre, and UA in serum and the 24-hour UTP mass concentration in urine (P＜0.05, P＜0.01). CDPs-L can significantly reduce the concentrations of BUN and UA in mouse serum. and 24 h UTP mass concentration in urine (P＜0.05, P＜0.01).
Cov lus 4 Teeb meem ntawm CDPS ntawm Bun, cre, ua rau 24 h utp hauv DN nas (n {2}})
| Pab | Bun concentration (MMOL / L) | Cree concentration (μmol / l) | 24h up cov ntsiab lus siab (mg / ml) | Ua ntej (μmmol / l) |
|---|---|---|---|---|
| Kev tswj hwm | 47.51±7.32 | 12.80±1.36 | 1.32±0.12 | 103.9±18.59 |
| Tus qauv | 157.97±30.57## | 44.85±4.36## | 2.38±0.37## | 500.43±81.06## |
| Cdps-l | 112.70±16.76* | 40.72±3.62 | 1.37±0.12** | 264.07±14.85** |
| Cdps-m | 82.31±14.68** | 35.37±6.21* | 1.06±0.17** | 247.62±11.22** |
| Cdps-h | 64.58±8.64** | 30.00±2.87** | 0.65±0.17** | 222.71±37.52** |
| Dapa | 76.98±10.47** | 28.82±3.72** | 0.37±0.10** | 129.87±17.69** |
2.3 Cov nyhuv ntawm CDPS ntawm cov khoom siv hluav taws xob thiab LPS kev cuam tshuam hauv cov dej hiav txwv ntawm C57BL / 6J nas
Raws li qhia hauv Table 5, piv nrog cov pab pawg tswj, cov ntshav siab ntawm TNF-,}}, tgf- thiab LPS hauv cov pab pawg tau nce ntxiv (p<0.01); compared with the Model group, Each dose of CDPs can reduce the levels of TNF-α, IL-1β, IL-6, TGF-β and LPS in serum, CDPs-M
Cov nyhuv ntawm CDPS-H pab pawg yog qhov tseem ceeb (P <{1}}. {}}.}. {6}}.}}.}}.}}.
Cov Lus 5 Qhov cuam tshuam ntawm CDPS ntawm cov khoom siv hluav taws xob thiab LPS hauv nas (n=6)
| Pab | Mass concentration (PG / ML) | ||||
|---|---|---|---|---|---|
| Tnf- | Il -1 | Il -6 | Tus hlauf- | LPS | |
| Kev tswj hwm | 111.23±5.47 | 125.85±11.41 | 112.27±10.28 | 152.25±7.28 | 116.17±15.07 |
| Tus qauv | 167.90±16.91## | 184.53±21.03## | 195.57±31.88## | 209.36±16.17## | 173.51±15.72## |
| Cdps-l | 144.57±10.15* | 160.46±11.84* | 169.10±20.32 | 189.03±8.02* | 151.41±9.40* |
| Cdps-m | 133.62±13.24** | 154.30±17.39* | 150.47±19.28* | 171.25±14.35** | 135.33±23.92** |
| Cdps-h | 119.30±7.31** | 140.54±16.11** | 127.12±10.94** | 169.47±10.57** | 124.98±16.38** |
| Dapa | 114.79±10.48** | 135.17±14.37** | 120.90±9.32** | 159.11±10.92** |
113.34±13.14**
|
2.4 Cov nyhuv ntawm CDPS ntawm lub raum pathology ntawm C57BL / 6J nas
Raws li muaj nyob hauv daim duab 1, tom qab nws steing, nws tau pom tias cov qauv glomerular yog li qub, lub raum tsis tu ncua, thiab tsis muaj mob ntawm tes ntawm tes. Piv nrog cov pab pawg tswj hwm, lub glomerular ntim ntawm cov nas nyob rau hauv tus qauv pab pawg tau infilding, thiab renal tubules tau cuam tshuam thiab dilated; Piv nrog rau cov qauv kev ua qauv, cov pab pawg CDPS muaj cov qib sib txawv ntawm kev txhim kho ntawm nas lub raum. Lub ntsej muag zoo nkauj ntawm cov nas hauv CDPS-M thiab CDPS-H pab pawg tau raug txo qis, thiab lub raum tsis zoo thiab tau teem kom nruj. Tom qab 4 lub lis piam ntawm CDPS kev cuam tshuam, cov kev mob phologological saum toj no tau rov qab los sib txawv ntawm cov qib sib txawv.
Qhov loj tshaj plaws pom tias lub raum ntawm cov nas nyob rau hauv pawg tswj hwm tau tshwm sim tau tshwm sim, thiab tubular basement jhaly waslranes tau pom; Qhov ntau ntawm cov txiv collagen loj tau pom nyob rau hauv Renal intersistial nqaij ntawm cov nas nyob rau hauv tus qauv cov nqaij mos yog pob thiab cov laus proliferated; Lub glomerular ntim ntawm cov nas nyob rau hauv CDPS-M Pab pawg tau raug txo, thiab cov tsos mob ntawm cov nqaij mos mos cov nqaij ntshiv tau txais txiaj ntsig; Lub raum roj liab ntawm cov nas hauv CDPS-H pab tau npaj ntau dua, thiab ib qho me me ntawm collagen fiber ntau proliferation tau raug pom.
PAS tso cov txiaj ntsig pom tau pom tias lub glomeruli ntawm cov nas hauv pab pawg tswj tau ib txwm nyob hauv cov qauv; Cov nas hauv pawg ua qauv muaj raum puas, lub glomerular ntim tau loj dua li ib txwm muaj thiab cov duab tsis xwm yeem, thiab Mesangial Matrix tau muab tso; Piv nrog rau cov qauv pab pawg, lub raum kev puas tsuaj ntawm pab pawg neeg cov tshuaj muaj kev pab cuam txhua yam, thiab qhov loj me ntawm Mesangial Matrix txo qis. Cov txiaj ntsig qhia tau tias CDPs tuaj yeem txo lub raum puas tsuaj rau hauv DN nas.
Daim duab 1 nyhuv ntawm CDPS ntawm cov kev hloov pauv ntawm lub teb chaws DN raws li kev tshuaj xyuas los ntawm H & E, Mass massing (× 400)
