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Cov teebmeem ntawm Cistanche deserticola ntawm lubmorphologythiab histochemistry ntawm testis thiab epididymis hauv nas

Wang Dejun, Sheng Shuqing, Liang Hong

【Cov Lus Qhia】 Lub Hom Phiaj: Saib xyuas cov teebmeem ntawmCistanche deserticolanyob rau hauv morphology thiab histochemistry ntawmqevthiabepididymishauv nas. Txoj Kev: Peb caug Kunming txiv neej nas raug xaiv thiab muab faib ua pawg Ⅰ, pab pawg Ⅱ, thiab pawg tswj hwm. Muab lub plab nrogCistanche deserticoladecoction (4g·kg -1·d-1 thiab 8g·kg -1·d-1), thiab pawg tswj tau gavage nrog ib txwm saline. Peb lub lis piam tom qab, cov nas raug txi thiab covphevsuav hauv cov phev raug txiav txim. Morphological ntsuas xws li viability, khiav ceev, thiab cov ntsiab lus ntawm fructose hauv seminal plasma. Histochemical kev soj ntsuam ntawmqevthiabepididymissuccinate dehydrogenase thiab non-specific lipase cov tshuaj tiv thaiv. Cov txiaj ntsig: Cov ntsuas saum toj no ntawm pawg tshuaj tau siab dua li ntawm pawg tswj hwm. Xaus: Nws qhia tiasCistanchedeserticolamuaj cov nyhuv ntawm kev ntxiv dag zog rau yang thiab nourishing lub raum.

Cistanche deserticolamuaj npe raws li ib tsob nroj ntawm tsev neeg Dang. Nws yog ib qho tseem ceeb heev tonic, uas muaj cov teebmeem ntawm nourishing lub raum, ntxiv dag zog rau yang, ntxiv dag zog rau lub siab thiab lub raum, thiab nourishing essence thiab qi. [1]Cistanche deserticolayog cov tshuaj feem ntau siv nyob rau hauv cov tshuaj noj rau lub raum thiab ntxiv dag zog rau yang yav dhau los. Nws kuj yog ib qho ntawm cov tshuaj tonic nto moo, thib ob tsuas yog ginseng [2]. Nyob rau hauv thiaj li yuav tshawb nrhiav ntxiv mechanism ntawmCistanchedeserticola, peb tau soj ntsuam morphology thiab histochemistry ntawm lubqevthiabepididymisntawm nas nyob rau hauv pawg tshuaj thiab pawg tswj.

Cistanche deserticola

1 Cov khoom siv thiab cov txheej txheem

1.1 Cov khoom siv

Tshuaj kho mob:Cistanchedeserticolatau tsim nyob rau hauv Alexa, Inner Mongolia, thiab tau txheeb xyuas los ntawm Inner Mongolia Institute for Drug Control, uas ua raws li cov cai ntawm National Pharmacopoeia. XovxwmCistanchedeserticolaExtract: dej=100g: 400ml, tsau rau 30min, tshav kub, thiab boil, ces Fry rau 30min nrog ib tug sov sov hluav taws, ncuav tawm cov tshuaj tov, thiab rov kib raws li qhov ratio ntawm 100g: 200ml. Tsom rau ob lub decoctions rau qhov sib npaug ntawm 1g crude tshuaj ib milliliter rau kev siv.

Tsiaj txhu: Kunming nas, 30 txiv neej, hnyav 25g -30g. Muab los ntawm Animal Center ntawm Yangzhou University School of Medicine.

Cistanchedeserticola tuaj yeem txhim kho cov phev zoo los ntawm kev noj haustestis thiab epididymis

1.2 Txoj kev

Cov tsiaj sim tau muab faib ua pawg Ⅰ thiab Ⅱ, nrog rau 10 tus tsiaj hauv txhua pab. Lawv muabCistanchedeserticoladecoction los ntawm gavage, thiab noj txhua hnub yog 4g / kg thiab 8g / kg, feem. Pawg tswj hwm (10 nas) tau muab tib lub ntim ntawm cov ntsev ib txwm los ntawm gavage. Nws yog siv ib zaug ib hnub rau 3 lub lis piam sib law liag. 24 teev tom qab kev tswj hwm zaum kawg, cov tsiaj tau txi.

1.2.1 Kev soj ntsuam nyob rau hauv lub microscope Coj nasepididymis[3], muab tso rau hauv 10ml ntawm 37 degree ib txwm salines, txiav mus rau hauv daim, sov da dej rau 10 feeb, tau txaisphevsuspension, thiab sau covphevQhov ceev raws li pom nyob rau hauv lub tshuab tsom xam raws li txoj kev suav cov qe ntshav dawb (D),phevkev muaj peev xwm (A),phevdeformity tus nqi (T) thiabphevkhiav ceev (V).

1.2.2 Kev txiav txim siab ntawm fructose cov ntsiab lus: coj nas phev siv resorcinol xim txoj kev [4], spectrophotometer ntawm 50 nm wavelength colorimetric, siv tus qauv fructose kos tus qauv nkhaus tom qab tib txoj kev kho, txiav txim siab cov ntsiab lus fructose hauv cov phev.

1.2.3 Kev soj ntsuam ntawm cov ntaub so ntswg: Coj nasqevthiabepididymis, kho nrog 4 formalin, embedded nyob rau hauv paraffin, seem, HE stain, soj ntsuam histological xwm txheej nyob rau hauv ib lub teeb tsom.

1.2.4 Kev soj ntsuam Histochemical: Coj nasqevthiabepididymis, kho nws nyob rau hauv 4 formalin, khov seem, saib cov kev hloov ntawm succinate dehydrogenase SDH thiab non-specific lipase NSE [5].

2 Kev tshwm sim

2.1 Cov txiaj ntsig ntawm Cistanche deserticola ntawm cov phev ntom ntom, muaj peev xwm ua tau, khiav ceev, thiab deformity tus nqi hauv cov phev

Table 1 Cov nyhuv ntawmCistanchedeserticolantawmphevD, A, T, V (x ± SD N=10)

2.2 Cov nyhuv ntawm Cistanche deserticola rau cov ntsiab lus ntawm fructose hauv nas phev

Table 2 Hloov cov ntsiab lus fructose hauv nas phev (mg / 100ml x ± SD)

Cov ntsiab lus ntawm fructose nyob rau hauv pab pawg neeg noj tshuaj yog ho siab tshaj qhov uas nyob rau hauv pawg tswj, P<>

2.3 Kev soj ntsuam Histomorphological

Pab pawg tswj: Lub hnab ntawv testicular yog du thiab ua tiav, thiab cov tubules seminiferous tuaj yeem pom nyob rau hauv ntu ntu ntawm cov tubules seminiferous. 4 mus rau 5 txheej ntawm spermatogonia thiab spermatocytes,phevcell, thiabphevnyob rau theem sib txawv ntawm txoj kev loj hlob yog teem nyob rau hauv ib qho kev txiav txim, tsim ntau yam morphology ntawm seminiferous epithelium dluab. Sertoli hlwb tuaj yeem pom thiab lawv cov morphology yog qhov qub. Muaj ob peb lub hlwb nyob rau hauv lub interstitium ntawm lubqev, thiab ob peb lub hlwb interstitial yog nyob rau hauv lub xeev zoo li fibroblast, nrog ntau vacuoles nyob rau hauv lub cytoplasm, lub teeb staining, thiab tsub zuj zuj ntawm lipid droplets thiab pigment. Lub thickness ntawm lub hauv paus membrane nyob ib ncig ntawm lub seminiferous tubules yog hais txog 6-8um

Cov tshuaj pab pawg: Cov tubules seminiferous tsis zoo, nrog lub thickness ntawm li 10 ~ 14um, thiab muaj cov leeg nqaij zoo li cov leeg nyob nruab nrab. Cov tsos ntawm cov tubules seminiferous yog ib txwm tsis tu ncua, nrog rau txhua theem ntawm cov spermatogenic hlwb nyob rau hauv ib qho kev txiav txim, txog 6 mus rau 8 txheej. Spermatogonia nyob ntawm lub hauv paus, chromatin yog nplua-grained, thiab staining yog tob. Muaj 1-2 qhov chaw me me nyob rau hauv lub nucleus nrog pom tseeb nucleoli. Tus naj npawb ntawm cov spermatocytes thawj zaug tau nce, thiab lawv qhov loj yog li 17um inch. Cov phev zoo li ib tug tadpole, nrog nws lub taub hau tig rau hauv qab daus daim nyias nyias thiab sib sib zog nqus staining. Testicular stromal hlwb raug faib ua pawg, tus lej nce, thiab cov hlab ntsha muaj ntau.

2.4 Cov txiaj ntsig ntawm Cistanche deserticola ntawm succinate dehydrogenase (SDH) thiab tsis yog lipase (NSE) hauv nas testis thiab epididymis

2.4.1 Succinate dehydrogenase (SDH): Muaj cov tshuaj tiv thaiv SDH nyob rau hauv cytoplasm ntawm spermatogenic hlwb ntawm txhua theem, cov spermatogonia muaj zog, thiab cov mesenchymal hlwb muaj cov tshuaj tiv thaiv zoo (Table 3). SDH-positive particles yog tawg nyob rau hauv epididymal tube epithelial hlwb, nrog rau ntau qhov chaw supranuclear. SDH cov lus teb ntawm pawg tshuaj muaj zog dua li ntawm pawg tswj hwm.

2.4.2 Non-specific lipase (NSE):qevthiabepididymismuaj cov tshuaj tiv thaiv zoo, tshwj xeeb tshaj yog cov noob qes stromal, thiab cov hlwb txhawb nqa tau zoo. Tus naj npawb ntawm cov khoom zoo nyob rau saum npoo ntawm cov kab mob epididymal duct epithelial hlwb hauv pawg tshuaj tau nce ntxiv (saib Daim duab 3).

Table 3 Kev sib piv ntawm cov tshuaj tiv thaiv kev siv ntawmCistanchedeserticolarau SDH thiab NSE ntawm nasqevthiabepididymis

3 Kev sib tham

Hauv qhov kev sim no, decoction ntawmCistanchedeserticolatau siv los muab cov txiv neej nas ntawm 4g / kg thiab 8g / kg rau 2 mus rau 3 lub lis piam. Covphevsuav thiab viability ntawm nas tau nce ntau, lubphevkhiav ceev nce, thiab covphevdeformity tus nqi yog qhov poob. Raws li kev soj ntsuam histomorphological, spermatogenic muaj nuj nqi ntawm covqevnyob rau hauv pab pawg tshuaj tau txhim kho, microenvironment ntawm lub epididymal raj tau txhim kho, thiab cov ntsiab lus fructose nce. Cov kev tshawb fawb histochemical tau pom tias SDH thiab NSE muaj txiaj ntsig zoo.

Spermatogenesis yog txheej txheem ntau theem, suav nrog peb theem: mitosis ntawm spermatogonia, maturation, thiab faib cov spermatocytes, thiab metamorphosis ntawmphevhlwb. Txhua theem muaj nws tus kheej tshwj xeeb hom metabolism. Kev faib tawm thiab spermatogenesis ntawm spermatogenesis ntawm txhua qib hauv cov tubules seminiferous xav tau cov as-ham txaus thiab kev tswj hwm ntawm cov tshuaj hormones endocrine. Cov tshuaj ib txwm ntawm lub teb chaws vam meej ntseeg hais tiasCistanchedeserticolamuaj cov txiaj ntsig zoo ntawm kev txhawb nqa lub raum thiab ntxiv dag zog rau yang, thiab tuaj yeem pom androgen thiab gonadotropin zoo li cuam tshuam los tswj kev ua haujlwm ntawm cov qog endocrine axis [6]. Androgens yog cov tshuaj hormones tseem ceeb hauv cov txheej txheem spermatogenesis. Covepididymisyog microenvironment rau kev loj hlob ntxiv, kev loj hlob, thiab kev sib txawv ntawmphev. Hauv cov txheej txheem no,phevtso siab rau cov nyhuv synergistic ntawm epididymal epithelium. Kev tso tawm, nqus, thiab cov khoom thauj khoom ntawm cov hlwb epithelial feem ntau yog siv los tswj kev txhim kho thiab kev sib txawv ntawmphevnyob rau hauv lub lumen, raws li zoo raws li cia thiab cia. Kev so microenvironment. Cov ntsiab lus ntawm fructose nyob rau hauv seminal plasma yog ib qho tseem ceeb ntawm kev xav txog kev ua haujlwm ntawm epididymal, thiab cov ntsiab lus ntawm fructose nyob rau hauv pawg tshuaj yog qhov siab dua li hauv pawg tswj hwm. SDH yog ib qho enzyme ntawm succinate oxidase system, uas yog teem rau hauv chains hauv mitochondria. SDH yog thawj enzyme hauv qhov system no. Nws muaj nyob rau hauv tag nrho cov hlwb ntawm aerobic respiration thiab yog ruaj khov rau lub mitochondrial membrane. Nws yog qhov tseem ceeb tshaj plaws dehydrogenase. Cov enzymes feem ntau yog siv rau hauv cov tshuaj histochemical los cuam tshuam cov xwm txheej ntawm lub voj voog tricarboxylic acid thiab dhau los ua cov cim enzymes. NSE kev ua haujlwm yog cuam tshuam nrog cov roj cholesterol hydrolysis thiab steroid synthesis thiab feem ntau yog siv los ntsuas kev ua haujlwm ntawm cov qe ntshav stromal hlwb [7]. Tus naj npawb ntawm cov tshuaj uas muaj cov tshuaj tiv thaiv zoo ntawm SDH thiab NSE hauv cov qog ntshav thiabepididymisntawm pawg tshuaj tau ntau dua li qhov hauv pawg tswj hwm, uas tau ua pov thawj tiasCistanchedeserticolaQhov tseeb tuaj yeem txhawb cov spermatogenesis ntawm testis thiab txhim kho microenvironment ntawm covepididymis. Nws muab ib tug txhim khu kev qha morphological hauv paus rau lub mechanism ntawmCistanche deserticolanourishing raum thiab aphrodisiac cuam tshuam.

cistanche deserticola txhim kho cov phev zoo

Cov ntaub ntawv

1. Li Zhou. Compendium ntawm Materia Medica. Beijing: People's Medical Publishing House, 1977: 727-728.

2. Huang Baomin, Li Xinwu. Kev tshawb nrhiav thiab kev tshuaj xyuas ntawm cov tshuaj thaum ub los tiv thaiv kev laus thiab kev ua neej ntev. Phau ntawv Journal of Hunan University of Traditional Chinese Medicine, 1987; 2(7):{5}}.

3. Kho los ntawm Li Yikui. Kev sim Methodology ntawm tsoos suav tshuaj Pharmacology. Shanghai: Shanghai Science thiab Technology Xovxwm, 509-511.

4. Luo Jianhua, tus thawj tswj hwm. Infertility Laboratory Diagnosis Technology. Xuelin Publishing House, 1994:44-46.

5. Chen Xiaomei. Phau ntawv Histochemistry. Beijing: People's Medical Publishing House, 1982: 130,224.

6. He Wei, Shu Xiaofen, Zong Guizhen. Kawm txog cov nyhuv ntawmCistanchedeserticolantawm nourishing raum thiab ntxiv dag zog rau yang ua ntej thiab tom qab ua. Suav Journal of Chinese Materia Medica, 1996; 21(9): 534-539.

7. Wang Yifei, Wu Jingmei, Qiu Yixuan. Histochemical ntsuas ntsuas kev ua haujlwm ntawm cov cell interstitial. Phau ntawv Journal of Cell Biology, 1980; 2(4): 27-30.