Cov nyhuv tiv thaiv ntawm Cistanche Glycoside ntawm kev raug mob spleen hauv cov nas raug hluav taws xob

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Cov txiaj ntsig ntawm Cistanche Glycosides ntawm kev raug mob spleen hauv cov nas raug hluav taws xob

Jiang Xiaoyan, Wang Xiaowen, Shang Xiaoying

Department of Pharmacology, Tsev Kawm Ntawv Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang

Abstract:

Aim: Qhov kev tshawb fawb no yuav musstudy tnws tiv thaiv cov nyhuvCistanche Glycosidentawm spleen raug mob nyob rau hauv cov nas raughluav taws xob.Txoj kev: Saib xyuas cov teebmeem ntawmCistanche Glycosidesntawm tus po index, selenium-glutathione-peroxidase (Se-GSH-Px) kev ua ntawm tus po, cov ntsiab lus ntawm malondialdehyde (MDA), histomorphology, thiab cov teebmeem ntawmhluav taws xobkev puas tsuaj rau nas. Raws li qhov cuam tshuam ntawm 30-hnub ciaj sia taus tus nas. Xaus: Tom qabhluav taws xobkev raug mob, tus spleen Performance index ntawm nas txo. Kev ua ntawm tus po Se-GSH-Px txo qis. Txawm hais tias MDA cov ntsiab lus nce ntxiv, morphology ntawm cov ntaub so ntswg yog qhov tsis zoo. Tus 30-hnub ciaj sia taus ntawm cov nas irradiated txo qis.Cistanche Glycosidestuaj yeem txhawb cov ntsuas saum toj no ntawm Kev Rov Qab. Cov txiaj ntsig: Cistanche Tag Nrho Glycosides muaj kev tiv thaiv kev raug mob spleen hauv cov nas irradiated. Nws cov txheej txheem yuav cuam tshuam rau cov teebmeem antioxidant.

Ntsiab lus: Cistanche Glycosides; raug mob plees; hluav taws xob; antioxidant nyhuv

Cistanche muaj cov teebmeem ntawm invigorating lub raum, replenishing essence, thiab aphrodisiac. Nws cov kev npaj tsis zoo tuaj yeem txhim kho SOD kev ua haujlwm ntawm cov qe ntshav liab nas, txo cov ntsiab lus ntawm myocardial lipofuscin, thiab ua kom muaj peev xwm ntawm tsiaj DNA synthesis hauv yang-deficiency tsiaj.Hluav taws xobkev tiv thaiv kev nqis tes ua/siv ⑶. Txawm li cas los xij, cov nyhuv tiv thaiv ntawmCistanche Glycosidesntawm spleen raug mob nyob rau hauv irradiated nas tsis tau qhia. Qhov kev sim no soj ntsuam cov teebmeem ntawmCistanche GlycosidesNyob rau ntawm tus po indexes, spleen biochemical indexes, thiab histomorphology ntawm nas puas los ntawm CoY rays, thiab lub hom phiaj los tshawb txog qhov muaj peev xwm tiv thaiv mechanism.Cistanche Glycosidesntawm nas spleen puas tsuaj los ntawm 6OCoY rays.

Cistanche

Nyem rau Cistanche NZ cov khoom

1 Cov khoom siv thiab cov txheej txheem

1.1 Tshuaj thiab tshuaj reagent

Cistanche Glycosidestau muab los ntawm Department of Phytochemistry ntawm Department of Pharmacy ntawm peb lub tsev kho mob; Huang Zhi (Astragalus Membranaceus, AM) txhaj tshuaj yog tsim los ntawm Shanghai Fuda Pharmaceutical Co., Ltd., 1,3, 3-tetra ethoxy propane (1,1, 3,3-tetra ethoxy propane, TEP ) yog ib yam khoom ntawm Sigma; (2-nitrobenzoic acid) [5, 5-dithiol-bis (2-nitrobenzoic acid), DTNB] yog Fluka Khoom; Coomassie Brilliant Blue (CBB) G 250 yog cov khoom lag luam Fluka; txo glutathione yog ib yam khoom ntawm Dongfeng Biochemical Technology Company, Shanghai Institute of Biochemistry, Suav Academy ntawm Sciences.

Cistanche Glycosides

1.2 Radiation tej yam kev mob

Tag nrho lub cev yog irradiated uniformly nyob rau hauv ib lub sij hawm, lubhluav taws xobkoob tshuaj npaum li cas yog 3.89X10-2Cy/kg, thiab cov nas nyob rau hauv pab pawg raug mob thiab txhua pawg tswj hwm ntawm qhov deb ntawm 0.80 mo tau txais koob tshuaj 2 Gy, uas yog siv rau kev soj ntsuam ntawm tus po index, 6 Gy Nws yog siv los soj ntsuam cov biochemical ntsuas ntawm tus po thiab 8 Gy yog siv los soj ntsuam cov 30-hnub ciaj sia taus tus nqi.

1.3 Tsiaj

NIH nas, lub cev hnyav (20 ntxiv rau 2) g, txiv neej thiab poj niam, muab los ntawm Xinzhao Epidemiological Research Institute, cov ntaub ntawv pov thawj ntau lawm yog Yidongzi No. 16-001, muab faib ua 5 pawg: (1) Ib pawg: Physiology Saline (NS) 20 ml / kg gavage, ib hnub ib zaug; (2) Pab pawg raug mob: NS 20 ml / kg gavage, ib hnub ib zaug; (3)Cistanche Glycosidespab pawg:Cistanche Glycosides62 ua . 5.125 mg / kg gavage, ib hnub ib zaug; (4) Cov pab pawg tswj tau zoo: Huangmao txhaj tshuaj (cov tshuaj crude, AM) 65 g / kg. gavage, ib zaug ib hnub.

1.4 Kev soj ntsuam cov ntsuas thiab cov txheej txheem kuaj

1.4.1 Kev txiav txim siab ntawm tus ntiv tes spleen hauv txhua pawg nas 5 hnub ua ntejhluav taws xob, ib hnub ib zaug, cov nas nyob rau hauv pab pawg raug mob thiab pawg thawj coj tau txais 2 Gy, thiab txhua pab pawg txuas ntxiv mus rau 6 hnub tom qabhluav taws xob, tom qab uas hnyav Cov nas raug txi coj tus po, thiab tus spleen hnyav tau hnyav. Qhov hnyav spleen / lub cev hnyav tau raug coj los ua tus spleen index (mg / g).

1.4. 2 Kev txiav txim siab ntawm tus kab mob spleen biochemical ntsuas thiab soj ntsuam histomorphological Cov nas nyob rau hauv txhua pab pawg tau txais kev noj zaub mov tsis tu ncua 7 hnub ua ntej thiab tom qab.hluav taws xob, ib hnub ib zaug. Cov koob txhaj tshuaj ntawm nas nyob rau hauv pab pawg raug mob thiab pawg thawj coj yog 6 Gy. Cov nas ntawm txhua pawg tau muab txi rau hnub 1, 3, 7, 14, thiab 21 hnub tom qabhluav taws xob, cov spleens tau noj, hnyav, yaug nrog dej khov NS, qhuav nrog cov ntawv lim, thiab ua 10 feem pua ​​(W/V) hauv cov dej khov da dej Cov homogenate tau centrifuged ntawm 3000 r / min rau 30 min, thiab cov supernatant raug coj mus rau kuaj. Tus nas spleen raug coj mus rau ntu pathological nyob rau hnub 7 tom qabhluav taws xob, thiab kev soj ntsuam histomorphological tau ua tiav. Cov ntsiab lus protein ntawm txhua qhov ntsuas ntsuas tau raug kho, thiab Se-GSH-Px kev ua haujlwm tau txiav txim los ntawm DTNB ncaj qha txoj kev1]; MDA tau siv los ntawm TBA colorimetric method⑸; Kev ntsuas cov protein tau ua los ntawm CBB-SDS txoj kev⑹.

1.4.3 Kev soj ntsuam ntawm 30-hnub ciaj sia taus tus nas tom qabhluav taws xob. Cov nas nyob rau hauv txhua pab pawg tau txais kev noj zaub mov tas li 5 hnub ua ntej thiab 7 hnub tom qabhluav taws xob, ib hnub ib zaug. Cov nas nyob rau hauv pab pawg raug mob thiab pawg thawj coj tau txais 8 Gy. Saib xyuas cov nas nyob hauv 30 hnub tom qabhluav taws xob, sau lub xeev ntawm cov nas thiab tus naj npawb ntawm cov neeg tuag txhua hnub, thiab suav cov 30-hnub ciaj sia taus tus nqi, kev txhim kho, thiab kev tiv thaiv cov nas raws li cov qauv hauv qab no.

1.5 Cov txheej txheem txheeb cais

Cov ntaub ntawv sim ntawm cov ntaub ntawv ntsuas tau qhia los ntawm G ± s), thiab tom qab kev xeem homogeneity ntawm qhov sib txawv, qhov kev xeem F yog siv; kev sib piv ntawm tus nqi ntawm ntau cov qauv ntawm cov ntaub ntawv suav siv qhov kev xeem chi-square.

2 Kev tshwm sim

2.1 Cov nyhuv ntawmCistanche Glycosides

Cov nyhuv ntawmCistanche Glycosides ntawm tus kab mob spleen ntawm cov nas tom qab kis tau, cov poov xab index ntawm nas poob tom qab raug, uas yog 43.94 feem pua ​​​​tsawg dua li ntawm pawg Zhengzhou (P<0.01), while="">Cistanche Glycosides62.5 mg / kg pawg, 125 mg / kg pawg, thiab AM pab pawg tuaj yeem txhawb kev rov qab los ntawm cov poov xab index, uas tau nce los ntawm 43.21 feem pua, 52.08 feem pua ​​thiab 52.63 feem pua ​​(P<0.01) compared="" with="" the="" injury="" group.="" the="" results="" suggest="" that="">Cistanche Glycosides thiab AM Nws muaj kev tiv thaiv los txo qhov hnyav ntawm tus po ntawm cov nas irradiated.

2.2 Cov teebmeem ntawmCistanche Glycosidesntawm Se-GSH-Px kev ua ub no thiab MDA cov ntsiab lus nyob rau hauv tus po ntawm irradiated nas

Piv nrog rau cov pab pawg ib txwm muaj, qhov kev ua ntawm Se-GSH-Px nyob rau hauv tus po ntawm cov nas tau txo qis rau thawj hnub tom qab kis tau, qhov qis tshaj plaws nyob rau hnub xya tom qab kis tau, cov ntsiab lus MDA tau nce, thiab qhov siab tshaj plaws ntawm xya. hnub tom qab kis tau (PVO. 05 ~ 0.01), nyob rau hnub 21 Lub ob koob tshuaj ntawmCistanche Glycosidesthiab pawg tswj xyuas qhov zoo tau muab piv nrog rau pawg raug mob. Cov kev ua ntawm Se-GSH-Px nyob rau hauv tus po tau nce thiab cov ntsiab lus ntawm MDA poob rau hnub 7 thiab 14 tom qab.hluav taws xob (P>0.05). P <0. 05-0.="" 01),="" the="" results="" show="" that="">Cistanche Glycosidestuaj yeem txhim kho lub peev xwm antioxidant ntawm spleen hlwb, txo lipid peroxidation kev puas tsuaj, thiab txhawb kev rov ua haujlwm ntawm splenic.

2.3 Cov nyhuv ntawmCistanche Glycosidesntawm histomorphology ntawm irradiated nas

Pleev Pleev Cov spleen nodules yog cov qauv ua tiav, cov spleen nodules loj, thiab cov hlwb yog tuab. Hauv pab pawg raug mob, cov qauv ntawm tus po nodules raug rhuav tshem, tus lej raug txo qis, cov hlwb tsis sib xws, thiab muaj edema thiab necrosis. Lub histomorphology ntawm lubCistanche Glycosidespawg thawj coj zoo ib yam li cov pab pawg ib txwm muaj. Cov spleen nodules tau ua tiav nyob rau hauv cov qauv, loj hauv cov naj npawb, ntim cov hlwb, me edema, thiab tsis muaj necrosis. Pawg Huangmao yog tib yam li covCistanche Glycosideskoob tshuaj pab pawg.

2.4 Cov nyhuv ntawmCistanche Glycosidesntawm 30-hnub ciaj sia tus nqi ntawm cov nas irradiated

Cov ciaj sia taus ntawm covCistanche Glycosidesob koob tshuaj pab pawg tau txhim kho (P<0.05~0.01) than="" the="" injury="" group,="" and="" the="" rate="" of="" improvement="" is="" respectively="" 21.21%="" and="" 30.30%,="" and="" its="" protection="" coefficient="" is="" all="" above="" 1.20,="" suggesting="" that="">Cistanche Glycosidesmuaj zoo duativ thaiv hluav taws xobkev puas tsuaj rau cov nas irradiated.

cistanche glycoside tiv thaiv hluav taws xob

3 Kev sib tham

Ionizinghluav taws xobtuaj yeem ua rau tag nrho cov molecules lom hauv lub cev kom ionize thiab excite, tshuaj bonds tawg, thiab ntau cov dawb radicals tsim, uas induces tsim lipid peroxides. Lub zog lipid peroxidation cov tshuaj tiv thaiv los ntawm ntau cov dawb radicals tuaj yeem ua rau cov nucleic acids, proteins, enzymes, thiab lwm yam. Biological macromolecules ua rau cov cell thiab cov ntaub so ntswg puas. Cov kev ua ntawm deoxyribonuclease nyob rau hauv tus po ntawm nas nce sharply tom qab tag nrho lub cevhluav taws xob, uas tuaj yeem ua rau kev tawg ntawm DNA. Lub deoxyribonucleic acid nyob rau hauv tus po yog heev puas thiab cov ntsiab lus yog heev txo. Cov ntaub so ntswg rhiab heev (xws li thymus, spleen, pob txha pob txha) lipid peroxidation khoom malondialdehyde nce ntau thiab nce nrog kev nce ntxiv ntawmhluav taws xobkoob. Hauv qhov kev sim no, nws tau pom tias qhov hnyav ntawm tus po ntawm cov nas tau txo qis tom qabhluav taws xob, cov kev ua ntawm tus po Se-GSH-Px raug txo, cov ntsiab lus ntawm MDA tau nce, morphology ntawm tus po cov ntaub so ntswg puas lawm, thiab 30-hnub ciaj sia taus tus nas raug txo.Cistanche Glycosidestuaj yeem txhawb cov ntsuas saum toj no ntawm Kev Rov Qab. Se- GSH-Px tuaj yeem tswj hwm qib HQ thiab txo cov tiam ntawm • OH ⑴. Tshem tawm cov khoom lipid peroxidation hauv cov khoomhluav taws xobtxheej txheem, indirectly inhibit qhov kev puas tsuaj ntawm lipid peroxidation decomposition khoom rau hauv nruab nrog cev thiab ntaub so ntswg, thiab tiv thaiv lub cev los ntawmhluav taws xobLub zog nce ntxiv ua rau muaj sia nyob ntawm cov nas irradiated rau 30 hnub. Kev tshawb fawb los ntawm Li Linlin et al. (3) qhia tiasCistanche Glycosidestuaj yeem txhawb kev rov qab los ntawm RNA thiab DNA cov ntsiab lus hauv tus po ntawm irradiated nas. Yog li ntawd, cov nyhuv tiv thaiv ntawmCistanche Glycosidesntawm spleen raug mob nyob rau hauv irradiated nas tej zaum yuav muaj feem xyuam rau cov nyhuv antioxidant ntawmCistanche Glycosidesthiab kev txhawb nqa ntawm splenic DNA thiab RNA synthesis.

Cov ntaub ntawv:

[1] Zhou Zhijin. Tshawb nrhiav kev nce qib ntawmCistanche]. Cov ntaub ntawv qhia txog tshuaj suav tshuaj, 1995,12(5):28,

[2] Wang Xu, Wang Xufei, Li Yuqi, et al. Tshawb nrhiav ntawmAnti-lipid peroxidation nyhuv ntawm Cistanche Glycosides. Northwest Pharmaceutical Journal, 1997, 12 (Ntxiv rau), 37.

[3] Li Xu, Wang Xu, et al. Anti-lipid peroxidation thiabKev tiv thaiv hluav taws xob los ntawm Cistanche Glycosides. Suav Journal of Chinese Materia Medica, 1997, 22(6); 364 ib.

[4] Xia Yiming, Zhu Lianzhen. Kev txiav txim siab ntawm glutathione peroxidase kev ua haujlwm hauv cov ntshav thiab cov ntaub so ntswg. DTNB direct method [J]. Hygiene Research, 1987, 16(4): 29.

[5] Chen Shunzhi. Jin Yau. Kev sib piv ntawm 3 txoj kev ntawm lipid peroxide TBA xim kev loj hlob]. Phau ntawv Journal of Clinical Laboratory Science, 1984, 2(4); 8.

[6] Macart M, Gerbaut L. Ib qho kev txhim kho ntawm coomassie xiav dye-binding txoj kev tso cai sib npaug rhiab heev rau ntau yam proteins; daim ntawv thov rau cerebrospinal kua [J]* Clin. Chem Acta, 1982, 112:93.

[7] Quan Hongxun. Zhang Qinxian. Kev sim kev tshawb fawb ntawm covtiv thaiv hluav taws xobnyhuv ntawmCistanche[J]. Intermediate Edition thiab 1993.24(8): 423.

[8] Jiang Tienan, Qi Heling, Li Zhiwang. Kawm txog lub ultrastructure ntawm tus po nyob rau hauv nas tom qab tag nrho lub cevhluav taws xob

Suav Journal of Radiological Medicine and Protection, 1983.3(5): 20.

[9] Zhou Xun Zhong Weiqiang, Fang Yunzhong. Cov teebmeem ntawm 7- kab tag nrho-lub cevhluav taws xobntawm lipid peroxidation nyob rau hauv cov ntaub so ntswg [Suav Phau Ntawv Xov Xwm ntawm Radiological Tshuaj thiab Tiv Thaiv, 1985, 5(6): 429;

[10] Chen Wei, Tus Thawj Saib Xyuas Haujlwm Zhou Mei. Free Radical Medicine [M] Beijing: Tib Neeg Tub Rog Kev Kho Mob Publishing House, 1991. 385.