Kev hloov pauv hauv kev ua haujlwm antioxidant thaum enzyme fermentation

TSHOOJ 4 Hloov hauv Kev Ua Mob Antioxidant thaum enzyme fermentation

Txiv hmab txiv ntoo yog nplua nuj nyob hauv cov as-ham thiab tuaj nyob rau ntau yam. Kev tsim cov tshuaj uas fermentation yog cov nyom thiab sib txawv, thiab muaj ntau txoj kev tshawb fawb ntawm lawvantioxidant kev ua siCov. Tib neeg ib txwm tshawb nrhiav thiab nrhiav pom, nrhiav cov khoom noj uas muaj txiaj ntsig zoo rauNeej Neeg Noj Qab Haus Huv, yog li enzymes tau dhau los ua lub ntsiab lus kub nyob rau xyoo tas los no. Cov txheej txheem enzyme ntau lawm yog kom tau cov enzymes los ntawm ntuj fermentation ntawm cov txiv hmab txiv ntoo thiab zaub. Raws li kev tshawb fawb dhau los, kab lus no ntxiv rau 4 tus kab mob muaj txiaj ntsig. Cov kab mob no nws tus kheej kuj muaj nyob hauv enzyme. Tom qab cov kab ntxiv ntxiv ntawm cov kab mob, tus naj npawb thiab hom microorganisms hauv cov txheej txheem fermentation hloov. Cov kev hloov pauv no yuav muaj dab tsi ntawm nws cov kev ua tshuaj antioxidant? Hauv tshooj dhau los, peb coj cov txiv av ua piv txwv rau cov lus qhia ntxaws ntxaws. Cov kev sib piv ntawm cov tshuaj antioxidant ntawm cov pab pawg sim thiab cov pab pawg tswj hwm ntawm cov enzyme hauv pawg sim ntau dua li ntawm pawg tswj hwm. Qhov siv ntawm cov haujlwm antioxidant tseem qhia txog kev ntseeg tau ntawm cov enzyme nyob rau hauv ib txoj kev.

Tshuaj ntsuab tshiab cistanche nrog muaj zog antioxidant zog

 

Cov kev pabcuam txhawb nqa ntawm wecistanche-tus tshiab cistanche exporter nyob rau hauv Suav teb:
Email: wallence.suen@wecistanche.com
WhatsApp / Tel: {+86 15292862950

 

Tshooj lus no muaj cov txiv hmab txiv ntoo ntau yam ntxiv thiab cov citrus rau kev sim thawj zaug, thiab cov ciav hlau thiab ntes cov kev hloov pauv hauv lawvantioxidant kev ua sithaum fermentation. Ntawm lub hauv paus ntawm kev txo nqi, los ntawm kev sim, peb vam tias yuav nkag siab ntau ntxiv tom qab cov kab mob thiab cov ntaub ntawv txhawb nqa rau cov qauv microbialy Artificial inoculation ntawm hlab.

 

 

 

4.1 Cov ntaub ntawv thiab cov hau kev

4.1.1 Ntaub Ntawv

(1) Cov ntaub ntawv sim

Siv cov txiv apples tshiab, pears thiab cov txiv hmab txiv ntoo nrog cov txiv hmab txiv ntoo tsis huv, tev lawv ib txwm muaj rau ntawm cov lus tsis huv, tev lawv thiab hlais lawv rau tom qab siv. Ntxiv cov piam thaj dawb thiab cov txiv hmab txiv ntoo rau lub khob sterilized iav ntawm ib pawg sib piv ntawm 1: 1. Ua kom cov kab mob uas xav tau rau kev sim thiab ua rau lawv tswj hwm tau rho tawm), muab nws tso rau hauv qhov chaw txias thiab qhuav. Coj cov qauv ntawm cov sijhawm sib txawv ntawm kev ua kom sov ntawm chav sov kom tau txhua cov kua enzyme muaj kua txiv ntoo kom muaj txiv hmab txiv ntoo thiab lim. Nqa supernatant raws li cov qauv kev sim, thiab siv nws ntsuas cov ntaub ntawv cuam tshuam tom qab centrifuging nws ntawm 10, {rpm nyob rau hauv lub siab-nrawm centrifuge rau 15 feeb. Nws yog tsim nyog sau cia tias nyob rau hauv cov txheej txheem ntawm ua enzymes, txhawm rau kom zam kev tsis tsim nyog muaj, tag nrho cov haujlwm yog nqa tawm hauv qab tsis muaj menyuam.

(2) cov twj siv |

Cov cuab yeej uas xav tau rau kev sim yog tib yam li 3.1.1 (2).

 

4.1.2 Cov hau kev

(1) hloov pauv hauv cov ntsiab lus phenol tag nrho thaum lub sijhawm fermentation

450 GRECT cov qauv tau ntxiv nrog cov dej distilled kom ua rau cov ntim 46mL, thiab tom qab ntawd 1ml ntawm folin-phenol reagent tau ntxiv. Sib tov zoo thiab hnov ​​mob rau 3min. Tom qab ntawd 3ml ntawm 20% naco3 tau ntxiv. Cov sib tov tau shaken nyob rau hauv ib qho dej sov tas li da dej da dej ntawm 25 degree rau 2H. Distilled dej tau siv raws li kev tswj ntsug. Qhov nqus tau ib qho yog ntsuas ntawm 760NM los ntawm Spectrophotometer. Peb qhov kev rov ua tiav tau ua rau txhua txoj kev kho mob. Tag nrho cov phenol cov ntsiab lus tau raug suav raws li cov kab sib npaug ntawm nkhaus.

(2) kev hloov pauv hauv lub zog txo thaum fermentation

Ntxiv 45 0 μl ntawm phosphate buffer nrog rau ib tug neeg muaj txiaj ntsig ntawm 6.5, ntxiv 2.5ml ntawm trichloroacetic acid (w / v) nrog kev sib nkag siab ntau ntawm 10%, centrifuge ntawm 3000 teev ntawm ferric chloride (w / v) nrog ib tug loj concentration ntawm 0.1%. Siv dej distilled raws li kev tswj dawb, thiab ntsuas kev nqus tau ib lub nthwv dej 700nm nrog lub spectrophotometer. Ua 3 replicates rau txhua qhov kev kho mob. Lub zog ntawm kev txo hwj chim tau txiav txim siab raws li qhov muaj txiaj ntsig.

 

(3) Kev hloov pauv hauv Superoxide anion radical lub peev xwm thaum fermentation
Place 4.5 mL of 0.{{10}}5 mol/L pH 8.2 Tris-HCl buffer in a constant temperature water bath and adjust the temperature to 25 degree . Tom qab 20 min, ntxiv 1 ml ntawm enzyme qauv tshuaj thiab 0.4 ml ntawm pyrogalol daws nrog cov moulol / l. Sib tov zoo thiab muab tso rau hauv 25 lub sijhawm dej da dej rau 5 min. Ntxiv 1.0 ml ntawm 8 mol / l hcl los txiav cov tshuaj tiv thaiv. Siv Tris-HCL Buffer raws li kev siv thiab siv lub spectrophotometer los ntsuas qhov nqus tau qhov nqus tau ib ntawm 299 nm los laij cov nqi sib txig. Cov pab pawg tswj tau dawb yog siv 1 ml ntawm cov kuab tshuaj siv cov qauv. Tus nqi Superoxide Super Radical Scaven raug suav nrog suav nrog cov qauv 4.1: {15} {23} { A2 yog qhov nqus ntawm cov enzyme qauv daws ntawm pawg sim.

 

(4) hloov pauv hauv Hydroxyl radical ua rau muaj peev xwm thaum fermentation

 

Ntxiv dej rau 45 {5}}}} μL piv txwv ntawm cov roj hmab loj ntawm cov roj ntsha Da dej ntawm 37 degree rau 1h. Xoom nrog dej distilled, thiab ntsuas kev nqus ib ntawm nthwv cua ntawm 562nm nrog lub spectrophotometer. Peb qhov kev rov ua tiav tau ua rau txhua txoj kev kho mob. Lub Hydroxyl Radical tus nqi tau suav raws li cov mis 4.2: Hydroxyl radical tus nqi (%)}) (4.2) qhov twg: A1 yog qhov nruab nrab ntawm seem; A2 yog qhov nruab nrab ntawm cov qauv daws teeb meem.

 

(5) Hloov nyob rau hauv DPPH dawb radical scavenging muaj peev xwm thaum fermentation
Qhov tseeb hloov 2 ml ntawm enzyme qauv tshuaj rau ib tug 1 {}}}}}}}}} dpph ethanol a ethanol a agolle concectation ntawm 2 10-4 mol / l. Sib tov zoo thiab cia sawv ntsug ntawm chav tsev kub rau 30 min. Siv 80% ethanol kev daws teeb meem raws li kev siv thiab ntsuas qhov nqus ntawm cov qauv ntawm 517 nm, uas tau sau ua A1. Mix 2 Ml ntawm Dpph daws thiab 2 ml ntawm 80% ethanol daws, thiab ntsuas lawv txoj kev nqus ntawm tib lub wavelength, uas tau sau ua A0. Mix 2 ml ntawm enzyme tov thiab 2 ml ntawm 80% ethanol daws, thiab ntsuas lawv txoj kev nqus ntawm tib lub nthwv dej, uas tau sau ua A2. Peb qhov kev rov ua tiav tau ua rau txhua txoj kev kho mob. Laij DPHPE DAWB RADILIC SCAVEGING RELIT raws li cov mis 4.3:
Dpph Free radical txaus luag tus nqi (%) {{{}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} A2 yog qhov nqus ntawm 2 ml enzyme daws thiab 2 ml 80% ethanol sib xyaw ua ke; A0 yog qhov nqus ntawm 2 ml dpph daws thiab 2 ml 80% ethanol sib xyaw daws teeb meem.

(6) kev hloov pauv hauv abts dawb radical txaus luag thaum fermentation
8 μL cov qauv tshuaj yog ntxiv rau 10 μl nrog phosphate tsis tau (5 mmol / l potassium sulfate thiab abs tov kab mob kom hnov ​​mob ntawm 30 degree thiab lub sijhawm tshuaj tiv thaiv ntawm 5 min. XOOM qhov kev daws nrog dej distilled thiab ntsuas kev nqus tau ib lub nthwv dej ntawm 734 nm siv lub spectrophotometer. Peb qhov kev rov ua tiav tau ua rau txhua txoj kev kho mob. Cov abts dawb radical funact tus nqi tau suav raws li cov mis 4.4:
Abts dawb radical txaus luag (%) =}}}} ... (4.4 yog qhov nqus tau ntawm pab pawg tsis muaj kev tiv thaiv; A2 yog qhov nqus ntawm cov pab pawg sim.