Cistanche Thiab Morinda Officinalis Ntawm Kev Kho Mob AVN

Tus sau:Guo Xuefeng, Hou Decai, Yu Rui

1. Liaoning University of Traditional suav tshuaj, Shenyang 110847;

2. Shenyang Orthopedic Tsev Kho Mob, Shenyang 110041;

3. Affiliated Tsev Kho Mob ntawm Liaoning University of Traditional suav tshuaj, Shenyang 110032

Abstract:Lub hom phiaj txhawm rau tshawb xyuas cov txheej txheem ntawmCistanchethiab Morinda officinalis tshuaj nyob rau hauv kev kho mob ntawmAVN(Avascular necrosis)raws li network pharmacology.

Nyem qhov no kom paub ntau ntxiv txog Cistanche Cov txiaj ntsig ntawm AVN

Methods: Lub active Cheebtsam ntawm lubCistanche- Morinda officinalis khub tau raug tshuaj xyuas los ntawm Cov Tshuaj Hauv Suav Tuam Tsev Pharmacology Database thiab Analysis Platform (TCMSP), thiab cov hom phiaj kab mob ntawm femoral taub hau necrosis tau muab los ntawm GeneCards thiab OMIM databases, thiab cov noob sib txuas tau txais thiab tsim siv Cytoscape 3.7.1. .

Lub koom haum-kab mob-lub hom phiaj kev tswj hwm kev sib txuas lus, raws li Database of Biological Information Annotation (DAVID), ua Gene Ontology (GO) functional enrichment analysis thiab Encyclopedia of Genomes (KEGG) pathway enrichment analysis for core targets.

Cov txiaj ntsig: Tag nrho ntawm 21 cov khoom siv nquag thiab 182 lub hom phiaj muaj peev xwm ntawm cov tshuaj Cistanche-Morinda Officinalis tau txais, thiab 727 lub hom phiaj cuam tshuam txog kab mob. GO enrichment tsom xam feem ntau suav nrog kev ua haujlwm xws li apoptosis, lipid metabolism tsis zoo, thiab cov kab mob oxygen reactive. txoj kev; KEGG txoj kev tsom xam feem ntau suav nrog hypoxia-inducible factor-1 signaling pathway, p53 signaling pathway, qog necrosis factor signaling pathway, etc.

Xaus: Raws li lub network pharmacology, tag nrho cov kev tswj xyuas ntawm Cistanche thiab Morinda officinalis ntawm kev kho mob ntawm femoral taub hau necrosis nrog ntau yam, ntau lub hom phiaj thiab ntau txoj hauv kev tau raug qhia ua ntej. Cov txheej txheem ntawm kev ua haujlwm tuaj yeem cuam tshuam nrog lipid metabolism tsis zoo, apoptosis thiab hypoxia induction. Qhov xwm txheej -1 txoj kev taw qhia thiab p53 txoj kev taw qhia txoj kev sib raug zoo.

Lo lus tseem ceeb:Avascular necrosis (AVN) ntawm lub taub hau femoral, network pharmacology, necrosis ntawm lub taub hau femoral, Cistanche,Cov txiaj ntsig Cistanche,Morinda officinalis

Osteonecrosis ntawm lub taub hau femoral yog ib qho kab mob sib koom ua ke los ntawm kev cuam tshuam los yog kev puas tsuaj rau cov ntshav ntawm lub taub hau femoral, uas ua rau muaj kev hloov pauv thiab ua haujlwm ntawm lub taub hau femoral. Cov txiaj ntsig ntawm kev sib kis tau pom tias muaj li ntawm 28 txog 30 lab tus neeg mob uas muaj necrosis ntawm lub taub hau femoral thoob plaws ntiaj teb thiab qhov kev nthuav dav tshiab tau nce xyoo los ntawm xyoo [1-2].

Txij li thaum pathogenesis ntawm femoral lub taub hau necrosis tsis tau qhia meej meej thiab nws yog ib qho kev loj hlob mus ntev, yog tias nws tsis tuaj yeem kuaj pom thiab kho thaum ntxov, nws yuav cuam tshuam rau lub neej zoo ntawm cov neeg mob, uas yog ib qho teeb meem kho mob uas yuav tsum tau daws. ceev ceev. Nrog rau kev hloov pauv ntawm hom kev kho mob, cov tshuaj suav tshuaj suav tshuaj rau kev kho mob ntawm femoral taub hau necrosis tau cuam tshuam los ntawm kev txhim kho rheological xwm txheej thiab femoral lub taub hau qauv, txhawb kev kho microvascular thiab rov tsim dua tshiab, thiab txo cov ntshav lipids. Nws yog dav siv nyob rau hauv kev tiv thaiv thiab kev kho mob ntawm femoral taub hau necrosis. Cov nyhuv zoo kawg nkaus.

Cistanche yog qhuav, scaly qia ntawm Cistanche nyob rau hauv tsev neeg Lidangaceae; Morinda officinalis yog cov rhizome qhuav ntawm Morinda officinalis, uas yog qab zib thiab pungent nyob rau hauv saj, me ntsis sov nyob rau hauv cov xwm, thiab rov qab mus rau lub raum thiab lub siab meridians.

Cov kev tshawb fawb tau pom tias Cistanche- Morinda officinalis muaj txiaj ntsig zoo rau kev kho mob femoral taub hau necrosis, tab sis vim yog qhov nyuaj ntawm cov khoom xyaw suav tshuaj, nws cov txheej txheem ntawm kev ua tsis tau meej meej. Raws li lub network pharmacology, cov khoom siv tseem ceeb ntawm cov tshuaj suav tshuaj suav tshuaj tuaj yeem txheeb xyuas tau, cov hom phiaj tuaj yeem pom, thiab cov tes hauj lwm tuaj yeem tsim nrog cov kab mob, uas zoo ib yam nrog ntau lub ntsiab lus thiab ntau lub hom phiaj ntawm cov tshuaj suav tshuaj. Raws li lub network pharmacology, peb tshawb nrhiav lub tshuab ntawmcistanche-morinda officinalis nyob rau hauv kev kho mob ntawm femoral taub hau necrosis, aiming qhia lub mechanism ntawm cistanche-morinda officinalis nyob rau hauv kev kho mob ntawm femoral taub hau necrosis los ntawm ib tug molecular taw tes ntawm view, thiab muab ib tug kev tshawb fawb kev taw qhia rau kev kho mob ntawm femoral taub hau necrosis nrog tsoos Suav. tshuaj kho mob. thiab scientific hauv paus.

Table 1 Database thiab tsom xam platform

Cov ntaub ntawv thiab cov txheej txheem rau Kev Kawm Cistanche Cov txiaj ntsig ntawm kev kho AVN

1.1 Database thiab Analysis Platform

Lub database thiab tsom xam platform tau piav qhia hauv Table 1.

1.2 Kev tshuaj ntsuam xyuas thiab lub hom phiaj kev kwv yees ntawm cov khoom xyaw nquag hauv Cistanche- Morinda Officinalis cov tshuaj khub los ntawm Traditional Chinese Medicine System Pharmacology Database thiab Analysis Platform (TCMSP), cov kev tshuaj ntsuam xyuas yog cov khoom zoo li tshuaj (DL) Ntau dua lossis sib npaug rau {{4} }.18, qhov ncauj bioavailability (OB) Ntau dua lossis sib npaug li 30 feem pua ​​, Kev tshuaj xyuas ua ntej ntawm cov khoom xyaw nquag ntawm Cistancheand Morinda Officinalis, thiab lub hom phiaj kev kwv yees ntawm cov tshuaj ntsuam xyuas cov khoom xyaw, ntxiv los ntawm cov ntaub ntawv nug los ntawm PubMed thiab CNKI databases.

Cov npe tshuaj lom neeg tau suav nrog siv Chemical Book thiab AiChem databases, thiab cov npe tshuaj ntsuam xyuas cov npe protein tau tsim qauv siv UniProt database.

1.3 Tau txais cov kab mob lub hom phiaj rau necrosis ntawm lub taub hau femoral

Siv "osteonecrosis ntawm lub taub hau femoral" raws li lo lus tseem ceeb, cov ntaub ntawv tshawb fawb suav nrog GeneCards thiab OMIM databases.

1.4 Core phiaj tshuaj ntsuam xyuas thiab PPI network tsim

Cov khub tshuaj Cistanche- Morinda Officinalis tau kos npe nrog lub hom phiaj femoral taub hau necrosis, thiab daim duab Venn tau siv kom tau txais kev sib tshuam los tshuaj xyuas lub hom phiaj tseem ceeb.

Kev sib cuam tshuam ntawm cov protein-protein (PPI) network ntawm lub hom phiaj tseem ceeb tau tsim los siv STRING database online platform, hom kev kawm tau teeb tsa rau tib neeg, thiab tus nqi sib cuam tshuam tau teeb tsa rau 0.400. Txhawm rau tsim kom muaj kev tswj hwm kev tswj hwm los nthuav qhia peb-dimensionally lub mechanism ntawm kev txiav txim ntawm Cistanche thiab Morinda Officinalis ntawm femoral taub hau necrosis.

1.5 GO enrichment tsom xam thiab KEGG txoj kev tsom xam ntawm cov hom phiaj tseem ceeb

Txhawm rau nthuav qhia ntxiv txog lub luag haujlwm ntawm cov phiaj xwm tseem ceeb hauv cov noob muaj nuj nqi, DAVID database tau siv los tshuaj xyuas GO enrichment ntawm lub hom phiaj tseem ceeb thiab KEGG txoj kev tsom xam. Cov hom tau txwv rau tib neeg, thiab qhov pib tau teem rau PValue<0.05. at="" the="" same="" time,="" the="" omicshare="" cloud="" platform="" was="" used="" to="" complete="" the="" data.="" results="" are="">

2 kev tshwm sim

2.1 Kev tshuaj xyuas cov khoom xyaw nquag thiab lub hom phiaj kwv yees ntawm Cistanche- Morinda Officinalis khub TCMSP tau tshuaj xyuas raws li DL thiab OB tsis, thiab tag nrho ntawm 25 cov khoom xyaw tseem ceeb ntawm Cistanche - Morinda Officinalis khub tau txais, tsis suav nrog 4 lub hom phiaj zoo uas tsis tau txais qhov sib xws. Lub hom phiaj cov khoom xyaw, thiab thaum kawg tau 21 cov khoom xyaw nquag, siv tau

Muaj 182 lub hom phiaj sib thooj rau cov khoom, thiab cov ntsiab lus tshwj xeeb thiab cov ntsiab lus cov ntsiab lus tau qhia hauv Table 2.

2.2 Tsim cov hom phiaj kab mob rau necrosis ntawm lub taub hau femoral

277 paub lub hom phiaj ntsig txog femoral lub taub hau necrosis tau kuaj pom los ntawm kev tshawb nrhiav GeneCards, thiab 453 lub hom phiaj paub txog femoral taub hau necrosis raug kuaj pom los ntawm OMIM database. Tom qab ua tiav, tag nrho ntawm 727 lub hom phiaj cuam tshuam tau txais thiab suav nrog hauv txoj kev tshawb fawb.

2.3 Kev tshuaj xyuas cov hom phiaj tseem ceeb ntawm Cistanche thiab Morinda officinalis rau kev kho mob ntawm femoral taub hau necrosis thiab kev tsim kho kev sib raug zoo network Siv Venn daim duab online platform, 727 kab mob lub hom phiaj ntawm femoral taub hau necrosis thiab 182 Cistanche thiab Morinda Officinalis tshuaj ntsuab tau siv los tshuaj xyuas cov nyhuv ntawm cov khoom xyaw nquag. Daim phiaj phiaj xwm tau siv los kos ib daim duab Venn. Raws li cov txiaj ntsig ntawm kev sib tshuam cov noob, 33 lub hom phiaj tseem ceeb rau kev kho mob femoral taub hau necrosis los ntawm Cistanche thiab Morinda officinalis tau txais (Daim duab 1). 33 lub hom phiaj tseem ceeb tau raug xa mus rau hauv STRING database online platform, PPI network qauv tau tsim, thiab PPI network cov ntaub ntawv ntawm cov hom phiaj tseem ceeb raug xa tawm (Daim duab 2).

Kev tswj hwm kev sib txuas tau tsim los siv Cytoscape 3.7.1 (Daim duab 3). Lub network muaj 54 nodes thiab 62 npoo. Hais txog cov khoom xyaw nquag, MOL000098 (quercetin) muaj 30 lub hom phiaj, thiab MOL000358 (beta-sitosterol) muaj 6 lub hom phiaj. Hais txog cov hom phiaj tseem ceeb, steroid receptor-activated protein 2 (NCOA2) tuaj yeem txuas rau 13 cov khoom xyaw nquag, thiab calcium-activated potassium channel protein 1 (KCNMA1) tuaj yeem txuas rau plaub cov khoom xyaw nquag. Qhov no qhia txog cov txheej txheem ntawm Cistanche- Morinda Officinalis ntawm ntau lub ntsiab lus thiab ntau lub hom phiaj kev kho mob ntawm femoral taub hau necrosis.

Daim duab 1 Venn daim duab ntawm lub hom phiaj tseem ceeb ntawm Cistanche thiab Morinda officinalis hauv kev kho mobAvascular necrosis (AVN) ntawm lub taub hau femoral

Daim duab 2 PPI network daim duab ntawm lub hom phiaj tseem ceeb ntawm Cistanche thiab Morinda officinalis rau kev kho mob ntawmAvascular necrosis (AVN) ntawm lub taub hau femoral

2.4 GO enrichment tsom xam ntawm cov hom phiaj tseem ceeb hauv kev kho mob femoral taub hau necrosis nrog Cistanche thiab Morinda officinalis

Los ntawm GO enrichment tsom xam, tag nrho ntawm 210 yam khoom ntsig txog cov txheej txheem lom neeg (BP), 34 yam khoom muaj feem xyuam rau kev ua haujlwm molecular (MF), thiab 20 yam khoom ntsig txog cellular muaj pes tsawg leeg (CC) tau txais (Daim duab 4 ~ Daim duab 7). Cov txiaj ntsig tau pom tias cov txheej txheem lom neeg ntawm Cistanche thiab Morinda officinalis hauv kev kho mob ntawm femoral taub hau necrosis feem ntau yog enriched nyob rau hauv cov tshuaj tiv thaiv lipid, reactive oxygen hom tshuaj tiv thaiv, apoptosis, thiab lwm yam. . Aminokinase thiab kev ua haujlwm loj hlob, kev hloov pauv hloov pauv, kev loj hlob zoo receptors, nuclear hormone receptors thiab kev hloov pauv hlau ion khi peev xwm; cell muaj pes tsawg leeg yog enriched nyob rau hauv lub membrane kab noj hniav thiab intimal system, platelet granules, secretory granules thiab

secretory vesicles, extracellular matrix, thiab lwm yam.

2.5 Lub hom phiaj noob ntawm Cistanche thiab Morinda officinalis hauv kev kho mob femoral taub hau necrosis

KEGG txoj kev tsom xam Los ntawm KEGG txoj kev tsom xam, tag nrho ntawm 67 KEGG nkag tau (Daim duab 8-Daim duab 9). Cov txiaj ntsig tau pom tias txoj hauv kev lom neeg ntawm Cistanche thiab Morinda officinalis hauv kev kho mob ntawm femoral taub hau necrosis feem ntau koom nrog p53 teeb liab txoj hauv kev, hypoxia-inducible factor-1 txoj kev taw qhia, qog necrosis factor signaling pathway, thiab interleukin-17 taw qhia txoj kev.

3 Kev Sib Tham ntawm Cistanche

Lub raum yog lub hauv paus ntawm lub hauv paus, feem ntau yog pob txha thiab pob txha. Yog hais tias lub raum essence txaus, pob txha pob txha yog biochemically active thiab muaj zog. Yog tias lub raum tsis txaus, cov pob txha pob txha tsis muaj qhov chaw biochemical, thiab cov pob txha tsis muaj zog thiab tsis muaj zog. Yog li ntawd, nyob rau hauv thaum ntxov thiab nruab nrab theem ntawm kev kho mob ntawm femoral taub hau necrosis, lub raum thiab pob txha loj hlob yuav tsum tau siv raws li lub ntsiab kev kho mob. Lub hauv paus ntsiab lus ntawm kev kho mob feem ntau yog raws li cov tshuaj suav tshuaj xws li Cistanche, Morinda officinalis thiab Epimedium.

Raws li daim ntawv thov ntau ntawm cov yam ntxwv ntawm omics thev naus laus zis, cov ntsuas kev ntsuas lub cev thiab tshuaj lom neeg, kev ntsuas kev ntsuas thiab tsis-coding RNA cov cim qhia hauv kev pom zoo ntawm TCM syndrome sib txawv ntawm femoral lub taub hau necrosis, nws muaj peev xwm los tshawb nrhiav tau zoo dua. ua rau TCM kev faib tawm ntawm femoral taub hau necrosis. Cov yam ntxwv kab mob. Network pharmacology tuaj yeem qhia meej txog lub peev xwm ntawm cov tshuaj suav tshuaj suav tshuaj hauv kev kho mob femoral taub hau necrosis. Los ntawm kev tsim lub "compound-kab mob-lub hom phiaj" kev tswj hwm network, nws muaj peev xwm txheeb xyuas cov txiaj ntsig ntawm cov tshuaj suav tshuaj suav nrog rau cov hom phiaj kab mob los ntawm cov khoom xyaw nquag ntawm cov tshuaj suav tshuaj suav tshuaj. Txoj kev loj hlob muab theoretical thiab technical support. Cistanche - Morinda Officinalis tshuaj khub yog ib qho tshuaj siv feem ntau hauv cov tshuaj suav tshuaj los ntxiv dag zog rau lub raum thiab ntxiv dag zog rau cov pob txha.

Hauv txoj kev tshawb no, 21 cov khoom xyaw tseem ceeb ntawm Cistanche - Morinda Officinalis khub tau txais los ntawm kev tshuaj ntsuam ua ntej, thiab 33 lub hom phiaj tseem ceeb ntawm Cistanche - Morinda Officinalis khub tau kuaj xyuas kev kho mob ntawm femoral taub hau necrosis. Lub mechanism ntawm kev txiav txim ntawm Morinda Officinalis nyob rau hauv kev kho mob ntawm femoral taub hau necrosis yog complex thiab muaj ntau haiv neeg, muaj ntau lub hom phiaj, es tsis yog ib lub hom phiaj. Yog li ntawd, cov khoom siv tau zoo ntawm Cistanche-Morida Officinalis cov tshuaj khub muaj cov txiaj ntsig zoo sib xws, thiab lub hom phiaj ua lub luag haujlwm tseem ceeb hauv kev kho mob femoral taub hau necrosis.

Kev soj ntsuam ntawm cov khoom xyaw nquag ntawmCistanche thiab Morinda officinalisqhia tiasquercetinmuaj ntau yam teebmeem pharmacological xws litiv thaiv kab mob, tiv thaiv qog, antioxidant, tiv thaiv kab mob, thiab lwm yam. Nws tuaj yeem tswj qhov sib npaug ntawm osteoblasts / osteoclasts thiab inhibit qhov degradation ntawm extracellular matrix. Raws li kev nthuav qhia ntawmpro-inflammatory yam, cuam tshuam inflammatory signaling pathways, lwm.

Daim duab 3 Daim duab kos duab ntawm kev sib txuas-kab mob-lub hom phiaj kev cai ntawmCistanchethiab Morinda officinalis rau kev kho mob ntawmAvascular necrosis (AVN)ntawm lub taub hau femoral

-sitosterol muaj ntau yam tshuaj xws li tshuaj tiv thaiv kab mob, tiv thaiv platelet aggregation, hypolipidemic, immunosuppressive, thiab lwm yam. Nws tuaj yeem ua lub luag haujlwm hauv cov pob txha metabolism los ntawm kev txhawb kev sib txawv ntawm zes qe menyuam granulosa hlwb thiab txhawb kev loj hlob ntawm osteoblasts. Kev cai. Lub hom phiaj tsom xam ntawm Cistanche thiab Morinda Officinalis cov tshuaj yeeb tshuaj tau pom tias KCNMA1 tuaj yeem tswj hwm los ntawm ob qho tib si membrane voltage thiab intracellular calcium ions, thiab tau nthuav dav hauv tib neeg osteosarcoma hlwb thiab osteogenic precursor hlwb. Cell proliferation thiab osteogenic sib txawv ntawm cov pob txha pob txha mesenchymal qia hlwb; qog necrosis factor- , interleukin -10, thiab interleukin -6 tau nce siab nyob rau hauv cov neeg mob uas muaj femoral taub hau necrosis, uas cuam tshuam nrog genetic susceptibility to femoral head necrosis Lub qog necrosis factor-gene polymorphisms rs1800629 thiab rs1800629, rs1800629 thiab rs1800629. thiab cov interleukin{10}} gene polymorphism rs1800872 tau cuam tshuam nrog qhov tshwm sim ntawm femoral taub hau necrosis;

Glucocorticoid-induced inhibition ntawm hypoxia-inducible yam tseem ceeb-1 plays lub luag haujlwm tseem ceeb hauv cov kab mob thiab kev loj hlob ntawm femoral taub hau necrosis; Kev hloov pauv kev loj hlob 1 tuaj yeem ua rau muaj kev tso tawm ntau dhau ntawm vascular endothelial kev loj hlob ntawm ib sab, ua rau cov pob txha fibrosis los tiv thaiv cov pob txha kho, Ntawm qhov tod tes, undifferentiated mesenchymal hlwb raug ntxias kom sib txawv rau hauv fibroblasts kom txo cov pob txha ntom.

Daim duab 4 Histogram ntawm GO function enrichment tsom xam ntawm Cistanche thiab Morinda officinalis hauv kev kho mob ntawmAvascular necrosis (AVN) ntawm lub taub hau femoral

GO enrichment tsom xam pom tias cov tshuaj ntawm Cistanche thiab Morinda feem ntau siv nws cov kev kho mob ntawm femoral taub hau necrosis los ntawm lipid metabolism, cell apoptosis, cytokine qhia, thiab reactive oxygen hom teb.

① Apoptosis: Qhov loj apoptosis ntawm osteoblasts tuaj yeem ua rau cov pob txha poob, thinning thiab txo cov pob txha trabecular, thiab kev hloov ntawm cov pob txha microstructure xws li pob txha pob txha thiab nyias cortical. Cov txheej txheem pathological no maj mam nce thiab cuam tshuam rau lub taub hau femoral. Cov ntshav ib txwm muab thaum kawg yuav ua rau avascular necrosis ntawm lub taub hau femoral. Serine / threonine-protein kinase phosphorylation thiab cov noob yog txhua yam tseem ceeb cuam tshuam rau cov pob txha cell apoptosis.

Cov kev tshawb fawb pom tau hais tias qog nqaij hlav protein p53 thiab Parkinson-related proteins tuaj yeem tiv thaiv kev ntxhov siab apoptosis thiab cell senescence ntawm cov pob txha pob txha mesenchymal qia hlwb los ntawm kev tswj cov mitophagy, thiab txhim kho kev kho cov pob txha pob txha mesenchymal qia hlwb ntawm cov steroid-induced necrosis ntawm femoral thaum ntxov. taub hau. Cov kev tshawb fawb tau pom tias Morinda officinalis polysaccharide, cov tshuaj nquag ntawm Morinda officinalis, tuaj yeem tswj hwm qhov sib npaug ntawm cov pob txha metabolism los ntawm kev txhawb kev sib txawv ntawm cov pob txha stromal hlwb rau hauv osteoblasts [25].

② Cytokine qhia: insulin zoo li kev loj hlob zoo, cov pob txha morphogenetic protein, vascular endothelial kev loj hlob yam, prostaglandin E2, platelet-derived kev loj hlob yam, transforming kev loj hlob thiab lwm yam loj hlob yam yuav txhawb femoral taub hau angiogenesis, txhim khu pob txha thiab pob txha proliferation thiab sib txawv ua si. lub luag haujlwm hauv kev kho mob ntawm femoral taub hau necrosis.

Tsis tas li ntawd, cov tshuaj hormones nuclear receptors xws li glucocorticoids, androgens, estrogens, thiab vitamin D, thiab transcription factor receptors xws li Runt-related transcription factors thiab forkhead box transcription factor O kuj koom nrog hauv kev tsim thiab kev loj hlob ntawm femoral taub hau necrosis. Cov kev tshawb fawb tau lees paub tias cov tshuaj siv tshuaj ntawm Cistanche tuaj yeem txhawb kev qhia ntawm pob txha morphogenetic protein 2 kom sib txawv rau hauv osteoblasts.

③ Lipid metabolism: lipid deposition tuaj yeem cuam tshuam qhov sib npaug thiab kho ntawm intra-osseous microcirculation, qhib lossis inhibit qhov kev hloov pauv ntawm osteogenic thiab adipogenic hlwb, ua rau muaj kev cuam tshuam ntawm cov roj metabolism thiab nws thiaj li ua rau femoral taub hau necrosis. Cov kev tshawb fawb tau pom tias qhov tsis txaus ntawm cov tshuaj tiv thaiv redox tuaj yeem ua rau lipid peroxidation ua rau osteoblast tsis ua haujlwm, kev tsis sib haum xeeb thiab apoptosis, thiab lipid metabolism tsis meej ua rau tsis muaj mob femoral taub hau necrosis thiab peroxisome proliferator ua kom. Lub cev taw qhia txoj hauv kev, calcium ion-txog kev taw qhia txoj hauv kev, thiab adenylate-activated protein kinase signaling txoj kev muaj feem cuam tshuam.

Cov duab ntawm Cistanche tubulosa

④ reactive oxygen hom teb: ntau hom pa oxygen tuaj yeem txhawb nqa vascular endothelial puas, txo cov pob txha hlwb thiab

Osteoblasts, kev ua kom tsis zoo ntawm osteoclasts, thiab txhawb kev degradation ntawm pob txha mos collagen fibers thiab proteoglycans, ua rau muaj kev cuam tshuam ntawm cov ntshav ntawm lub taub hau femoral, kev puas tsuaj rau cov qauv kev txhawb zog, flattening thiab vau ntawm lub taub hau femoral hauv cheeb tsam kev ntxhov siab, thiab thaum kawg femoral taub hau necrosis.

Daim duab 5 GO function enrichment tsom xam ntawm Cistanche thiab Morinda officinalis hauv kev kho mob ntawmAvascular necrosis (AVN) ntawm lub taub hau femoral- BP tsom xam npuas daim ntawv

Fig. 6 GO function enrichment analysis of Cistanche deserticola thiab Morinda officinalis hauv kev kho mobAvascular necrosis (AVN) ntawm lub taub hau femoral- MF tsom xam npuas daim ntawv

Daim duab 7 GO function enrichment tsom xam ntawmCistanchethiab Morinda officinalis hauv kev kho mob ntawmAvascular necrosis (AVN) ntawm lub taub hau femoral- CC tsom xam npuas daim ntawv

KEGG txoj kev tsom xam pom tau tias Cistanche - Morinda Officinalis cov tshuaj khub feem ntau ua rau femoral taub hau necrosis los ntawm p53 signaling pathway, interleukin -17 signaling pathway, hypoxia-inducible factor-1 signaling pathway, qog necrosis factor signaling pathway, thiab lwm txoj kev taw qhia. Cov nyhuv kho.

①p53 kev taw qhia txoj hauv kev: Raws li cov qog nqaij hlav, p53 tuaj yeem ua rau apoptosis los ntawm kev tswj hwm txoj hauv kev endogenous apoptosis, txoj kev tuag teeb liab receptor protein, thiab mitochondrial tso tawm cov pa dawb radicals. Ua kom muaj kev cuam tshuam los ntawm ib puag ncig, p53 kev taw qhia txoj hauv kev ua lub luag haujlwm tseem ceeb hauv femoral lub taub hau necrosis los ntawm kev tswj hwm apoptosis thiab lub voj voog ntawm tes. Cov kev tshawb fawb tau pom tias p53 kev qhia tuaj yeem nce ntxiv hauv necrosis ntawm lub taub hau femoral.

②Hypoxia-inducible yam tseem ceeb-1 txoj kev taw qhia: Txoj hauv kev hypoxia-inducible -1 txoj kev taw qhia yog qhov tseem ceeb hauv lub cev cov lus teb rau qis oxygen concentration lossis hypoxia. Qhov ib puag ncig hypoxic ntawm femoral taub hau necrosis tuaj yeem cuam tshuam rau osteoclastogenesis, thiab hypoxia-inducible yam Lub -1 txoj kev taw qhia tuaj yeem ua ob peb angiogenesis, pob txha resorption thiab cov pob txha tshiab tsim kom muaj txiaj ntsig kho. Cov kev tshawb fawb tau pom tias hypoxia-inducible factor-1 tuaj yeem koom nrog hauv cov txheej txheem ntawm angiogenesis los ntawm kev kho cov vascular endothelial kev loj hlob, thiab tib lub sijhawm, nws tuaj yeem ua lub luag haujlwm hauv kev tswj cov khoom noj khoom haus thauj thiab cuam tshuam rau kev ua haujlwm ntawm osteoclasts, yog li. ua rau femoral taub hau necrosis thiab pob txha resorption disorders.

③Tumor necrosis factor signaling pathway: TNF ua lub luag haujlwm hauv femoral taub hau necrosis los ntawm kev khi rau nws cov receptor. Cov kev tshawb fawb tau pom tias qhov kev qhia ntawm cov qog nqaij hlav necrosis tseem ceeb - yog qhov nce ntxiv hauv steroid-induced necrosis ntawm lub taub hau femoral hauv pawg luav qauv, thiab polymorphism ntawm qog necrosis factor-gene thiab hypoxic ib puag ncig tuaj yeem cuam tshuam rau kev soj ntsuam cov txiaj ntsig ntawm hormonal-induced necrosis ntawm lub taub hau femoral. kev loj hlob. Raws li kev kho mob feem ntau tshwm sim, qog necrosis factor- plays lub luag haujlwm tseem ceeb hauv kev puas tsuaj ntawm vascular endothelial hlwb ntawm lub taub hau femoral, thiab tseem tuaj yeem cuam tshuam cov ntshav ntawm lub taub hau femoral los ntawm intravascular coagulation; Nws kuj tuaj yeem ua rau kev nqus ntawm cov pob txha pob txha thiab chondrocytes. Nws tuaj yeem txhawb kev loj hlob ntawm fibroblasts; Nws tuaj yeem ua rau muaj cov pa dawb radicals, lipid peroxidation, apoptosis ntawm osteoblasts thiab lwm yam txheej txheem pathological, thiab kev cuam tshuam ntawm cov kev sib txuas saum toj no yuav ua rau muaj qhov tshwm sim ntawm femoral taub hau necrosis.

Daim duab 8 Histogram ntawm KEGG pathway enrichment tsom xam ntawmCistanchethiab Morinda officinalis hauv kev kho mob ntawmAvascular necrosis (AVN) ntawm lub taub hau femoral

Daim duab 9 Bubble chart of KEGG pathway enrichment analysis ofCistanchethiab Morinda officinalis hauv kev kho mob ntawmAvascular necrosis (AVN) ntawm lub taub hau femoral

Txhawm rau ua kom tiav, Cistanche thiab Morinda officinalis muaj cov yam ntxwv ntawm ntau cov khoom xyaw nquag, ntau lub hom phiaj, thiab ntau txoj hauv kev hauv kev kho mob femoral taub hau necrosis, nrog rau txoj hauv kev lom neeg nrog rau p53 taw qhia txoj hauv kev, hypoxia-inducible yam {{2 }} signaling pathway, qog necrosis yam signaling pathway, interleukin-

17 Txoj kev teeb liab, thiab lwm yam, cov txheej txheem lom neeg muaj xws li apoptosis, cytokine qhia, lipid metabolism, reactive oxygen hom teb, thiab lwm yam kev ua haujlwm. Cov txiaj ntsig ntawm txoj kev tshawb no muab lub hauv paus rau kev tshawb fawb tom ntej ntawm cov txheej txheem ntawm Cistanche thiab Morinda officinalis hauv kev kho mob.Avascular necrosis (AVN) ntawm lub taub hau femoral. 

NRHIAV LUB BOTANIKAL HOLISTIC CARE AVN

Peb ua tib zoo xuas tes ua los tiv thaiv daim siab cov mis suav nrog cov tshuaj tshawb fawb zoo tshaj plaws thiabcov khoom cistanche. Txhua qhov qhia tau hais tias muaj peev xwm zoo rau kev tswj hwm kev tiv thaiv kab mob, ntxiv rau cov paj hlwb thiab kev txhawb nqa neuron tag nrho.

Xws li:

Tnws Cistanche Cov txiaj ntsig:– Cistanche extract tsis cuam tshuam rau osteoclastogenesis. Tsis tas li ntawd, Cistanche extract tiv thaiv cov pob txha poob vim los ntawm ovariectomy hauv vivo. cistanche tej zaum yuav yog ib tug tshiab pob txha tsim tus neeg sawv cev rau kev kho mob ntawm AVN thiab osteoporosis