Kev Tshawb Fawb Suav Tshawb Pom Cov Qauv Crystal Thiab Catalytic Mechanism Ntawm MbnBC Holoenzyme Yuav Tsum Tau Rau Methanotrophic Biosynthesis

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Methanobactins (Mbns) yog ib tsev neeg ntawm tooj liab-binding peptides koom nrog hauv kev nce ntawm tooj liab los ntawm methanotrophs thiab yog cov muaj peev xwm kho tau cov kab mob uas tshwm sim los ntawm kev puas tsuaj tooj liab. Mbns yog tsim los ntawm kev hloov kho ntawm MbnA precursor peptide ntawm cysteine residues catalyzed los ntawm cov tub ntxhais biosynthetic machinery suav nrog MbnB, hlau-dependent enzymes, thiab MbnC. Txawm li cas los xij, cov ntsiab lus ntawm kev siv tshuab ntawm MbnBC holoenzyme catalysis tseem tsis meej.

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Thaum Lub Ob Hlis 2, 2022, ib daim ntawv tshawb fawb hu ua "Crystal qauv thiab catalytic mechanism ntawm MbnBC holoenzyme xav tau rau methanobactin biosynthesis" tau luam tawm hauv online hauv Cell Research los ntawm Cheng Wei pab neeg los ntawm Sichuan University, uas qhia tau hais tias MbnABC complexes los ntawm ob hom sib txawv. Cov qauv siv lead ua ntawm lub substrate tau qhia tias tus thawj coj peptide ntawm lub substrate MbnA khi rau MbnC los nrhiav MbnBC holoenzyme, thaum lub ntsiab peptide ntawm MbnA nyob rau hauv lub catalytic kab noj hniav tsim los ntawm MbnB-MbnC kev sib cuam tshuam thiab muaj ib qho tshwj xeeb triiron pawg.

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Qhov txuas ntawm substrate sulfhydryl rau lub chaw triiron ua rau cov tshuaj tiv thaiv dioxygen-dependent rau oxazolone-thioamide installation. Kev txheeb xyuas cov qauv ntawm MbnABC complex thiab kev ua haujlwm ntawm MbnB variants tau txheeb xyuas qhov kev khaws cia catalytic aspartate residue raws li xav tau rau MbnBC-mediated hloov kho ntawm MbnA. Hauv kev xaus, txoj kev tshawb no nthuav tawm cov qauv zoo sib xws thiab kev ua haujlwm ntawm MbnBC complexes los ntawm ntau hom, ua kom pom kev hloov pauv ntawm kev txuag catalytic mechanism ntawm MbnBC holoenzyme.

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Cov hlau xws li tooj liab yog qhov tseem ceeb rau kev tswj hwm lub cev homeostasis hauv txhua yam kab mob thiab tshwj xeeb yog koom nrog hauv catalysis ntawm qee cov kab mob tseem ceeb. Txawm hais tias tooj liab xav tau rau kev ua haujlwm ntawm qee cov proteins, nws kuj tseem siv los ua cov tshuaj tua kab mob hauv ntau hom kev lag luam thiab kev kho mob. Hauv kev teb, cov kab mob tuaj yeem raug tshem tawm los ntawm kev siv tooj liab-chelating tebchaw xws li Chalkophore. Chalkophore molecules zoo ib yam li cov hlau-binding cab kuj koom nrog hauv cov kab mob metabolism thiab detoxification. Qhov tseem ceeb, lawv tau kawm hauv kev sim tshuaj raws li qhov muaj peev xwm kho tau rau Wilson's kab mob, kab mob caj ces uas ua rau ntau dhau ntawm cov tooj liab hauv lub cev (lub siab thiab lub hlwb).

Methanoxins (Mbns) yog Chalkophore nkaus xwb tam sim no hauv methanotrophs. Ob peb Mbns tau tus cwj pwm thiab pom tias yog ribosomally synthesized thiab post-translationally modified peptides (RiPPs). Mbn molecules yog biosynthesized los ntawm genetic encoded precursor polypeptides (MbnAs), uas muaj ib tug thawj coj peptide (LP) rau kev lees paub los ntawm biosynthetic machinery thiab ib tug tub ntxhais peptide (CP) uas dhau los ua cov khoom mature tom qab MbnA hloov kho thiab LP cleavage. Ib lub tshuab biosynthetic tseem ceeb uas cuam tshuam nrog MbnBC complex tau pom tsis ntev los no hauv Methylosinus trichosporium (Mt) OB3b.

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Txog niaj hnub no, tsib pawg gene-encoding pawg (I-V) tau ua rau Mbn biosynthesis. Hauv kev paub Mbn operons, mbnA ib txwm ua raws los ntawm mbnB thiab mbnC noob lub luag haujlwm rau MbnA hloov kho. mbnB yog kwv yees kom encode ib triosephosphate isomerase (TIM) thiab qhia MbnA-hloov kev ua si tsuas yog thaum complexed nrog MbnC. Txawm li cas los xij, lub koom haum thiab catalytic mechanisms ntawm MbnBC machinery tseem tsis paub.

Ntawm no, txoj kev tshawb fawb tau txiav txim siab cov qauv siv lead ua ntawm RrMbnABC thiab VcMbnABC complexes ntawm Rugamonas rubra (Rr) ATCC 43154 (RrMbnBC, sawv cev pawg III) thiab Vibrio caribbenthicus (Vc) BAA-2122 (VcMbnBC, sawv cev rau pawg V). Cov qauv ntawm RrMbnABC thiab VcMbnABC tau khaws cia thiab muaj ib qho tshwj xeeb triiron pawg txuas rau cysteine residues ntawm substrate MbnA. Cov txiaj ntsig ntawm txoj kev tshawb no nthuav tawm cov txheej txheem los ntawm MbnBC holoenzyme catalyzes tsim Mbn.

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