Kev Tshawb Fawb Kev Tshawb Fawb Thiab Daim Ntawv Thov hauv Fingerprint Technology Ntawm Suav Materia Medica Ⅱ
Sep 19, 2024
1.2 Electrochemical ntiv tes
Electrochemistry yog ib txoj hauv kev yooj yim analytical raws li cov khoom siv hluav taws xob electrochemical thiab cov ntsiab lus ntawm cov khoom yuav tsum tau kuaj, uas yog tus nqi qis, rhiab heev, thiab yuav tsum tau siv tsawg [19]. Txawm li cas los xij, txoj kev no muaj qee qhov kev txwv thiab tam sim no feem ntau siv rau kev soj ntsuam ntawm Suav tshuaj uas muaj cov tshuaj lom neeg xws li terpenes (xws li Angelica sinensis), flavonoids (xws li Scutellaria baicalensis), alkaloids (xws li Aconite), thiab anthraquinones ( xws li Rhubarb) uas tuaj yeem ua rau cov tshuaj tiv thaiv electrochemical.
Wu Di et al. [20] siv tus ntiv tes electrochemical los tsim cov txheej txheem zoo tshaj plaws ntawm Scutellaria baicalensis Georgi, muab txoj hauv kev zoo rau kev siv cov khoom siv tshuaj hauv Suav teb. Shi Hui et al. [21] siv Belousov-Chabotinsky (BZ) cov tshuaj tiv thaiv tshuaj oscillation thev naus laus zis los tshawb xyuas qhov cuam tshuam ntawm qhov kub thiab txias, kev sib hloov ceev, thiab cov khoom siv tshuaj ntxiv rau cov txiaj ntsig kev sim thiab tsim cov kev sim tsim nyog kom tau txais electrochemical ntiv tes ntawm Magnolia biondii Pamp. ., muab txoj hauv kev tshiab rau kev tswj xyuas zoo ntawm Magnolia biondii. Zou Guihua et al. [22] tsim cov nonlinear electrochemical ntiv tes ntawm Codonopsis pilosula (Franch.) Nannf. Thiab Gentiana macrophylla Pall. Cov ntiv tes electrochemical ntawm ob cov khoom siv tshuaj yog qhov pom kev sib txawv, uas tuaj yeem siv los ua ib txoj hauv kev yooj yim thiab siv tau los paub qhov txawv ntawm Codonopsis pilosula los ntawm Gentiana macrophylla. Dai Hongxia et al. [23] ua ib qho kev tshuaj xyuas electrochemical ntawm 20 pawg ntawm Angelica sinensis (Oliv.) Diels nrog lub sijhawm loj hlob ntawm 1, 2, thiab 3 xyoo. Ua ke nrog cov ntsiab lus tseem ceeb, nws tau pom tias cov ntiv tes electrochemical thiab oscillation tsis (xws li oscillation induction lub sij hawm, lub peev xwm siab tshaj plaws, lub neej oscillation, siab tshaj plaws amplitude, tsis muaj peev xwm, thiab pes tsawg lub voj voog) ntawm Angelica sinensis ntawm lub sij hawm loj hlob txawv txawv, uas tuaj yeem siv los ua lub hauv paus rau kev txheeb xyuas Angelica sinensis ntawm ntau lub sijhawm.
CHINESE MATERIALMEDICAL HERB CISTANCHE HPLC FINGERPRINT
1.3 Chromatographic ntiv tes
1.3.1 Nyias txheej chromatography
Nyias-txheej chromatography yog ib txoj kev chromatographic uas ua tiav kev sib cais los ntawm kev cia siab rau qhov sib txawv adsorption muaj peev xwm ntawm tib lub adsorbent rau cov tshuaj sib txawv. Nws yog txoj kev siv ntau tshaj plaws rau kev txheeb xyuas cov tshuaj suav tshuaj, nrog rau qhov zoo ntawm kev ua haujlwm yooj yim, cov khoom siv yooj yim, zoo rhiab heev, kev sib piv siab, muaj zog tshwj xeeb, thiab kev tsim xim yooj yim. Qhov tseeb ntawm cov khoom siv tshuaj tuaj yeem txiav txim siab los ntawm qhov muaj lossis tsis muaj pob ntawm cov phaj nyias nyias. Qhov tob thiab qhov loj ntawm cov xim ntawm cov pob tuaj yeem cuam tshuam qhov zoo ntawm cov khoom siv tshuaj rau qee yam [24]. Tam sim no, kev ua haujlwm siab nyias-txheej chromatography(HPTLC) feem ntau yog siv los hloov cov kua chromatography (HPLC) kom muaj txiaj ntsig zoo thiab muaj nuj nqis txheeb xyuas cov khoom lag luam suav tshuaj vim nws qhov yooj yim, raug, tus nqi qis, kev ua haujlwm siab, thiab ceev ceev. HPTLC cov ntiv tes muaj kev daws teeb meem zoo dua thiab tuaj yeem kwv yees cov khoom xyaw nquag hauv cov tshuaj hauv lub sijhawm luv dua [25]. Txawm li cas los xij, vim muaj ntau yam cuam tshuam (humidity, kub, thiab lwm yam), kev tsim tawm ntawm cov txiaj ntsig tsis zoo piv rau HPLC. Loescher et al. [26] siv HPLC thiab HPTLC los tshuaj xyuas cov extract ntawm Calendula officinalis thiab pom tias HPLC yog qhov tsim nyog rau kev soj ntsuam ntau dua li HPTLC.
Nyob rau hauv teb rau cov nyhuv no, Li Xiangjun li al. [27] tau ua ntau txoj kev daws teeb meem, suav nrog kev ua cov kev sim kev txhim kho hauv lub tub yees lossis lub tshuab kaw qhov ntsuas kub kom txo tau qhov kub thiab txias thiab ntxiv qhov sib txawv ntawm sulfuric acid ntawm ib kawg ntawm lub tog raj kheej txoj kev loj hlob los tswj cov av noo.
CHINESE MATERIAL MEDICAL HERB CISTANCHE HPLC LABLE
Patil et al. [28] tsim HPTLC ntiv tes ntawm cov roj av ether extract ntawm Catsia tora Linn. Cov txiaj ntsig tau pom tias cov nplooj, cov noob, thiab paj muaj 10, 7, thiab 11 hom khoom xyaw, feem. Txoj kev no tuaj yeem siv los txheeb xyuas qhov tseeb ntawm Catsia tora Linn. Xiao Honghua et al. [29] siv nyias txheej chromatography ua ke nrog pawg spectrometry los tsim ib txheej nyias chromatographic ntiv tes ntawm chromones, organic acids, thiab triterpenoid saponins nyob rau hauv Cimicifuga foetida L., thiab ua qhov zoo sib xws tsom xam, pawg tsom xam thiab cov ntsiab lus tsom xam ntawm 16 pawg ntawm Cimicifuga kuaj los ntawm tsib qhov chaw, muab lub hauv paus rau kev tswj hwm zoo ntawm Cimicifuga.
Guzelmeric et al. [30] siv ethyl acetate-formic acid-acetic acid-dej (30: 1.5: 1.5: 3) ua cov kuab tshuaj los tsim HPTLC ntiv tes ntawm apigenin -7-glucoside, ib qho cim cim ntawm Matricaria chamomilla L., uas tuaj yeem siv los ua cov lus qhia tseem ceeb rau kev soj ntsuam zoo ntawm chamomile. Bazylko et al. [31] tsim HPTLC ntiv tes ntawm tsib lub ntsiab lus flavonoid, uas yog tagetoides, quercetin, flavonoids, caffeic acid, thiab chlorogenic acid, los ntawm Galinsoga parviflora Cav. Txoj kev no tuaj yeem siv los txheeb xyuas Galinsoga parviflora. Ethanolic thiab al. [32] tsim cov ntiv tes ntawm ethanol extract ntawm Acacia catechu (L. f.) Willd. Siv HPTLC tau ua qhov kev ntsuam xyuas yooj yim thiab nrawm ntawm rutin thiab quercetin hauv cov extract. Agatonovic-Kustrin et al. [33] siv HPTLC kom muaj nuj nqis txheeb xyuas apigenin, Matricaria, thiab bisabolol hauv nplooj thiab paj rho tawm ntawm Pyrethrum parthenium (L.) Smith, Matricaria chamomilla L., thiab Calendula officinalis L., ua ke nrog DPPH txoj kev los sib piv cov tshuaj antioxidant. muaj peev xwm ntawm cov khoom no. Txoj kev no yog qhov yooj yim, nrawm, txhim khu kev qha, thiab pheej yig, thiab tseem tuaj yeem siv los tshuaj xyuas cov haujlwm antioxidant ntawm cov nroj tsuag rho tawm.
1.3.2 HPLC txoj kev HPLC yog ib qho kev tshuaj ntsuam xyuas thoob ntiaj teb rau kev kuaj xyuas ntau yam tshuaj hauv cov tshuaj suav tshuaj.
Nws yog dav siv hauv kev tshawb nrhiav cov tshuaj lom neeg hauv cov tshuaj suav tshuaj vim nws qhov zoo ntawm kev kub siab, siab rhiab heev, kev ua haujlwm siab thiab automation [34]. UV ntes, diode array detector (DAD), electrochemical detector, evaporative light detector, thiab lwm yam.
Qiao et al. [35] siv reversed-theem × thim rov qab theem ua tiav ob-dimensional kua chromatography (RP × RP 2DLC) los cais cov phenolic tebchaw tau los ntawm ethyl acetate extraction ntawm licorice Glycyrrhiza uralensis Fisch.
311 cov tebchaw tau kuaj pom nyob rau hauv 40 feeb, thiab cov qauv ntawm 21 yam tsis paub cov tebchaw tau pom ua ntej los ntawm pawg spectrometry, thiab 8 ntawm lawv tau pom nyob rau hauv licorice thawj zaug. Cov txiaj ntsig tau pom tias RP × RP2DLC / MS system muaj cov txiaj ntsig zoo ntawm kev sib cais ntawm cov tshuaj suav tshuaj nyuaj thiab kev tshuaj xyuas zoo ntawm cov khoom ntuj tsim. Peng Liang et al. [36] txheeb xyuas cov flavonoid Cheebtsam ntawm 10 batch ntawm Gynostemma pentaphyllum (Thunb.) Makino tsim HPLC ntiv tes ntawm Gynostemma pentaphyllum thiab ntsuas 11 cov yam ntxwv siab tshaj plaws. Cov txiaj ntsig kev txheeb xyuas qhov zoo sib xws tau pom tias qhov zoo sib xws ntawm 10 batches ntawm Gynostemma pentaphyllum ntiv tes txheeb ze rau qhov siv spectrum yog 0.989, thiab qis tshaj yog 0.128, qhia tias flavonoid Cheebtsam ntawm Gynostemma pentaphyllum los ntawm ntau lub hauv paus chiv keeb tau zoo sib xws hauv hom tab sis muaj qhov sib txawv tseem ceeb hauv cov ntsiab lus. Sun et al. [37] siv HPLC-DAD txoj kev los tshuaj xyuas qhov zoo sib xws ntawm cov tshuaj lom neeg ntawm Bupleurum chinense DC. thiab B. scorzonerifolium Willd. Thawj zaug, thiab pom tias ob qho tib si muaj 12 lub ncov flavonoid thiab 4 saponin peaks, raws li, thiab tib lub sijhawm, txhua tus muaj 6 lub ncov flavonoid thiab 5 saponin peaks, raws li. Cov txiaj ntsig tau pom tias txoj kev no tuaj yeem cuam tshuam qhov sib txawv ntawm cov tshuaj lom neeg ntawm Bupleurum thiab Bupleurum chinense. Qhov kev sib raug zoo ntawm ob yog qhov tsis zoo. Yog li ntawd, Bupleurum chinense yuav tsum tau siv nrog ceev faj hauv kev kho mob. Nws et al. [38] quantitatively analyzed 10 yam bioactive gallic acid, chlorogenic acid, caffeine, thiab catechins muaj nyob rau hauv 10 batch ntawm Ziyang ntsuab tshuaj yej. HPLC ntiv tes tsim tau yooj yim thiab txhim khu kev qha. Li et al. [39] tsim HPLC ntiv tes ntawm pomegranate tev. Lawv xaiv 15 tus yam ntxwv ncov thiab ntsuas qhov zoo sib xws ntawm 10 pawg ntawm pomegranate tev kom yog 0.968. Lawv kuj tau sib cais thiab txheeb xyuas ntau yam ntawm punicalagin, gallic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, thiab ellagic acid muaj nyob rau hauv nws, muab ib txoj kev txhim khu kev qha thiab zoo rau kev txheeb xyuas qhov tseeb thiab kev tswj xyuas ntawm pomegranate tev. Sumathy et al. [1] siv HPLC ua ke nrog HPTLC thiab roj chromatography-mass spectrometry (GC-MS) los txiav txim cov tshuaj muaj pes tsawg leeg ntawm methanol extract ntawm Ixora chinensis Lam. HPLC tsom xam pom muaj biochin A, myricetin, quercetin, rutin, daidzein, thiab formononetin; ursolic acid tau pom nyob rau hauv HPTLC ntiv tes; 24 phytochemical Cheebtsam tau pom los ntawm GC-MS; Txoj kev sib xyaw ua ke zoo thiab ntau yam tuaj yeem ntsuas qhov zoo thiab ruaj khov ntawm Ixora chinensis Lam. Ge et al. [40] tsim lub UHPLC-DAD ntiv tes ntawm Radix Angelicae Pubescentis, quantitatively analyzed coumarins thiab phenolic acids muaj nyob rau hauv Radix Angelicae Pubescentis, thiab qualitatively txheeb xyuas nws cov khoom xyaw nquag siv quadrupole-lub sij hawm ntawm davhlau huab hwm coj spectrometry (Q-TOF-MS). Lawv pom 9 phenolic acids, 30 coumarins, thiab 41 tebchaw ntawm myrrh thiab adenosine. Lawv tau ua ke txoj kev txheeb xyuas kev ntxub ntxaug kom paub qhov txawv 32 pawg ntawm Radix Angelicae Pubescentis los ntawm ntau lub xeev. Txoj kev no muab lub tswv yim tshiab rau kev tswj xyuas zoo ntawm Radix Angelicae Pubescentis.
CISTANCHE SPECS LIST
Yang et al. [41] siv ultra-high performance kua chromatography (UPLC)-DAD detector thiab ESI-MS thev naus laus zis kom tau txais HPLC ntiv tes thiab cov ntiv tes loj ntawm Xiaoyanlidan Tablets, feem. Lawv tau soj ntsuam 113 cov qauv ntawm Xiaoyanlidan ntsiav tshuaj thiab tau txais 39 lub ncov, ntawm 26 yog los ntawm Quassia, 9 los ntawm Andrographis paniculata, thiab 4 los ntawm Rhizoma Cynoglossi. Siv cov txheej txheem chemometric zoo sib xws thiab PCA, lawv tau txiav txim siab tias tsib yam tseem ceeb yog 4-methoxy-5-hydroxyferrocephalostomone, andrographolide, dehydroandrographolide, neoandrographolide, thiab rosmarinic acid. Lawv tau tsim cov txheej txheem thiab cov ntiv tes ua ke los txhim kho thiab ntsuas qhov zoo ntawm Xiaoyanlidan Tablets. Fan et al. [42] tsim HPLC thiab UPLC cov ntiv tes ntawm flavonoid glycosides hauv Lophatherum gracile Brongn., thiab cais ib qho tshiab flavonoid C-glycoside luteolin 6-C- -D-glucuronic acid-(1→2){19 }}D-pyranoglucoside thawj zaug. Lawv kuj nrhiav pom cov isomers optical ntawm ob lub tebchaw, flavonoid flavonoid thiab flavonoid hydroxyflavonoid, thawj zaug. Lawv siv ib-dimensional thiab ob-dimensional nuclear magnetic resonance thiab huab hwm coj spectrometry kom paub qhov txawv thiab txiav txim lawv cov qauv, muab ib txoj kev tshiab rau kev tswj zoo ntawm Lophatherum gracile Brongn. Tshibangu et al. [43] siv HPLC-DAD ua ke nrog nyias txheej chromatography, nuclear magnetic resonance, thiab huab hwm coj spectrometry kom zoo cais lub ntsiab biflavonoid compounds GB1, GB2, GB-1a thiab flavonoids los ntawm Garcinia kola Linn., thiab paub tseeb tias lawv cov qauv. , uas tuaj yeem siv los ua ib qho kev soj ntsuam niaj hnub rau kev npaj tshuaj. Zhao et al. [44] tsim HPLC-UV-MS ntiv tes ntawm Andrographis paniculata (Burm. f.) Nees, quantitatively txiav txim siab andrographolide thiab dehydroandrographolide muaj nyob rau hauv Andrographis paniculata, thiab ntsuam xyuas qhov sib txawv ntawm 10 batch ntawm Andrographis paniculata los ntawm kev tsom xam thiab PCA sib xws. Txoj kev no muab lub hauv paus rau kev tswj kom zoo ntawm Andrographis paniculata.
Ahmed et al. [45] siv HPLC kom tau txais cov ntiv tes ntawm Commiphora wightii (Arn.) Bhandari resin, thiab txheeb xyuas qhov ntau ntawm cov trans- thiab cis-sterolone uas muaj nyob hauv nws. Nyob rau tib lub sijhawm, txoj kev no tuaj yeem paub qhov txawv ntawm 22 pawg ntawm Commiphora wightii resin thiab 9 pawg ntawm cov khoom qias neeg, uas tuaj yeem siv los ua ib txoj hauv kev los txheeb xyuas qhov tseeb ntawm Commiphora wightii resin.
2 Cov ntiv tes lom neeg
Biological fingerprints yog siv cov txheej txheem soj ntsuam cov noob nyob rau theem molecular kom paub qhov txawv thiab faib thaj chaw faib thiab hloov pauv hauv Suav cov ntaub ntawv tshuaj, suav nrog cov ntiv tes genomic, cov ntiv tes proteomic [60], thiab DNA ntiv tes [61]. Tam sim no, muaj tsawg dua kev tshawb fawb txog cov ntiv tes lom neeg ntawm cov tshuaj suav tshuaj ntau dua li cov ntiv tes tshuaj lom neeg. Cov kev siv feem ntau yog polyacrylamide gel electrophoresis (PAGE), random amplified polymorphic DNA molecular marker technology (RAPD), polymerase chain reaction.
(PCR) thiab lwm txoj hauv kev.
Liu Li et al[62] siv RAPD cov cim, ua ke nrog PCR thiab kev txheeb xyuas pawg, txhawm rau txheeb xyuas Panax notoginseng (Burk.) f. H. Chen, Panax ginseng CA Mey., thiab American ginseng Panaxquinquefolium L. Fan Wei et al. [63] thawj zaug tsim SDS-PAGE protein ntiv tes ntawm Eupolyphaga sinensis Walker, Bombyx mori Linnaeus, thiab Scolopendra nyiaj pab mutilans L. Koch, muab ib txoj hauv kev txhim khu kev qha thiab zoo rau kev txheeb xyuas ntawm peb cov khoom siv tshuaj. Wei Yicong et al. [64] tsim dual-site tshwj xeeb primers thiab tsim ib tug multiplex PCR Kev qhia ceev ceev ntawm ob yam khoom siv tshuaj, Houttuynia cordata Thunb. Thiab Gymnotheca chinensis Decne., tuaj yeem txheeb xyuas ob hom zoo thiab txiav txim siab seb lawv puas sib xyaw. Wang Fu et al. [65] siv ITS2 (sab hauv transcribed spacer 2) raws li DNA barcode kom raug txheeb xyuas cov tshuaj suav, Zanthoxylumbungeanum Maxim. Thiab nws cov txuj lom Zanthoxylumbungeanum Maxim., muab lub hauv paus rau kev txheeb xyuas ntawm Suav tshuaj Zanthoxylumbungeanum thiab nws cov txuj lom Zanthoxylumbungeanum.
