Tshooj 5 Kev hloov pauv hauv enzyme kev ua thaum lub sij hawm enzyme fermentation ⅱ

5.2 Cov Ntsiab Lus thiab Kev Tshawb Xyuas

5.2.1 Superoxide dismutase (sod)

Pib txij thaum pib ntawm fermentation, tusSuperoxide dismutase (Sod) kev ua ntawm Apple enzymetau ntsuas txhua 15 hnub, thiab cov kev ua si hloov nkhaus ntawmSuperoxide dismutase (sod)Ntawm cov sijhawm sib txawv ntawm lub sijhawm sib txawv lub sijhawm tau, raws li qhia hauv Fig.5.1.

Fig.5.1 Kev nkhaus ntawm cov haujlwm ntawm SuperSoxide dismutase (sod)

Raws li muaj nyob hauv Fig.5.1, kev ua si ntawm cov khoom plig los ntawm cov khoom plig ua ntej nce thiab tom qab ntawd txo qis nrog ncua sijhawm fermentation. Thiab cov kev ua yeeb yam enzyme ntawm pawg sim tau qis dua li ntawm pawg tswj. Nws yuav yog tias cov kab ke sib tw nrog cov txheej txheem fermentation thiab cov tshuaj thaum cov kev ua si ntawm cov kev ua si ntawm cov kev ua si ntawm qee yam kev ua si ntawm cov enzymes. Piv nrog ntxiv ntawm cov leeg, cov kev ua ntawm sod enzyme poob qis los ntawm 22.39% nyob rau hnub 90 ntawm fermentation.

Cov tshuaj ntsuab tshiab nrog cov tshuaj tiv thaiv oxidation

 

 

 

 

5.2.2 amylase

Txij thaum pib ntawm fermentation, amylase kev ua ntawm Apple enzyme tau raug ntsuas txhua lub sijhawm 152, thiab cov kev ua si hloov pauv ntawm cov sijhawm sib txawv lub sijhawm tau, raws li muaj nyob hauv daim duab 5.2.

 

Raws li muaj nyob hauv Daim Duab 5.2, cov kev ua haujlwm amylase ntawm pawg tswj hwm nce hauv thawj 45 hnub ntawm fermentation, thiab poob qis tom qab 45 hnub. Ntawm 45 xyoos ntawm fermentation, amylase kev ua tau mus txog qhov siab tshaj plaws, uas yog 45U / ML; Cov amylase hauv pab pawg sim ua rau muaj nce qib siab los ntawm kev pib fermentation mus rau 60 hnub ntxiv tom qab 60 hnub. Nyob rau 60 hnub ntawm fermentation, cov haujlwm enzyme tau mus txog qhov siab tshaj plaws, uas yog 76U / ml. Amylase tau tsim tawm thaum lub cev nqaij daim tawv hauv pawg sim, thiab cov kev ua ntawm amylase yuav tsis raug txo vim yog lwm yam laj thawj. Piv nrog cov leeg yam tsis muaj ntxiv ntawm cov leeg, cov kev ua si amylase nce los ntawm 50% nyob rau hnub 90 ntawm fermentation.

Cov tshuaj ntsuab tshiab nrog cov tshuaj tiv thaiv oxidation

5.2.3 lipase

Txij thaum pib ntawm fermentation, Lipase kev ua si ntawm Apple enzyme tau raug ntsuas txhua txhua lub sijhawm li 15 hnub, thiab cov kev hloov pauv hloov ntawm lub sijhawm sib txawv ntawm cov sijhawm sib txawv

Fig.5.3 Txoj kev nkhaus ntawm cov haujlwm ntawm lipase

 

Raws li muaj nyob hauv Daim duab 5.3, muaj qhov sib txawv loj hauv daim duab las ntawm ib pab pawg uas muaj kev sim thiab pawg tswj hwm. Hauv pab pawg tswj, lub pas dej lasase nce ntau nyob rau hauv thawj 30 hnub, thiab tom qab ntawd maj mam poob rau hauv 45 hnub tom ntej; Lipase kev ua haujlwm ntawm pawg kws sim tau qhia txog kev hloov pauv nce li 45 hnub, tsis ruaj khov ntawm 45 hnub, thiab pom ib qho tseem ceeb ntawm 55 hnub. Lipase cov ntsiab lus ntawm cov pawg sim tau siab los ntawm 45 hnub, thaum lub sijhawm tswj hwm qis dua thaum lub sijhawm no, thiab cov rog rog loj zuj zus. Cov yam ntxwv ua haujlwm ntawm cov enzymes muaj sib txawv hauv cov sijhawm sib txawv. Piv nrog cov ntaub ntawv tsis muaj kab mob, cov lassase kev ua si ntawm rooj plaub uas muaj cov kab mob ntxiv los ntawm 69.49% nyob rau hnub nyoog 90th ntawm fermentation.

Cov tshuaj ntsuab tshiab nrog cov tshuaj tiv thaiv oxidation

5.2.4 Protase

(1) nkhaus nkhaus ntawm Tyrosine yog qhia hauv Daim Duab 5.4.

Raws li qhia hauv Daim duab 5.4, qhov sib npaug ntawm cov qauv nkhaus ntawm tyrosine yog y {}}. 9963, uas y yog qhov nqus tau thiab x yog cov qauv pom zoo.
(2) Kev txiav txim siab ntawm cov kev ua txhaum ntawm Apple enzyme ntawm cov kev ua si sib txawv ntawm cov kev ua haujlwm ntawm cov kev ua haujlwm ntawm cov kev ua haujlwm ntawm cov sijhawm sib txawv, raws li qhia hauv Daim duab. 5.5.

Fig.5.5 Kev nkhaus ntawm cov haujlwm ntawm procease

Raws li muaj nyob hauv Daim duab 5.5, kev ua kev ua txhaum txawv txav heev thaum lub sijhawm fermentation txheej txheem. TusKev ua protease siab tshaj plawsNtawm pawg kev sim yog nyob rau 45th hnub, nrog kev ua si enzyme ntawm 890U / ml. Cov kev ua si ntawm cov pab pawg tswj hwm yog qhov siab tshaj plaws nyob rau 15 hnub ntawm fermentation, nrog ntau kawg ntawm 655u / ml. Saib xyuas tag nrho cov txheej txheem fermentation tuaj yeem muab siv rau cov yam ntxwv ua haujlwm ntawm cov enzyme. Piv nrog fermentation yam tsis muaj cov kab mob ntxiv, cov kev ua kom muaj protease nce los ntawm 85.71% ntawm 90th hnub fermentation.

 

5.2.5 Cellulase

(1) Curvve nkhaus ntawm cov dej qab zib yog qhia hauv Daim Duab 5.6.

Fig.5.6 Tus qauv nkhaus ntawm cov piam thaj

Raws li pom hauv Daim duab. 5.6, qhov sib npaug ntawm cov qauv nkhaus ntawm cov piam thaj yog y {}. 10687}. 10687}.
(2) Pib txij thaum ntxov ntawm kev ua haujlwm, lub cellulase kev ua si ntawm Apple enzyme tau ntsuas txhua lub sijhawm ntawm cov sijhawm sib txawv ntawm cov sijhawm 5.7.

Fig.5.7 Txoj kev nkhaus ntawm cov haujlwm ntawm cellulase

Raws li muaj nyob hauv Daim duab 5.7, lub cellulase kev ua ntawm cov pab pawg sim ib txwm yog siab dua li ntawm pawg tswj thaum lub sijhawm fermentation txheej txheem. Lub cellulase kev ua ntawm cov pab pawg sim tau mus txog qhov siab tshaj plaws nyob rau 30 hnub ntawm fermentation, nrog ntau kawg ntawm 63.7U / ml; Lub cellulase kev ua ntawm pawg tswj hwm tau ncav qhov siab tshaj plaws ntawm 45 xyoos ntawm fermentation, nrog ntau tshaj ntawm 47.9U / ml. Piv nrog fermentation tsis muaj cov kab mob uas tsis muaj cov kab mob cellulase tau nce los ntawm 54.19% nyob rau hnub 90 xyoo ntawm fermentation.

 

 

5.3 Cov ntsiab lus ntawm tshooj no

Tshooj lus no siv Apple enzyme ua ib qho piv txwv los kawm cov kev hloov pauv ntawm cov txheej txheem kev sim thiab cov pab pawg tswj tau thaum lub sijhawm fermentation txheej txheem. Kev sim ua qhia tau hais tias muaj qhov sib txawv ntawm cov kev ua ntawm cov microbial enzymes ntawm cov khoom noj khoom haus hauv Inoculation ntawm cov hlab ntsha thiab ntuj fermentation. Sod enzyme kev ua tau qhia txog kev hloov ntawm thawj nce thiab tom qab ntawd txo qis ntawm fermentation lub sijhawm, thiab cov kev ua si enzyme ntawm pawg sim tau qis dua li ntawm pawg tswj hwm. Cov kev ua yeeb yam amylase tau nce hauv thawj 45 hnub ntawm fermentation nyob rau hauv pab pawg tswj thiab poob qis tom qab 45 hnub; Nyob rau hauv thawj 60 hnub ntawm cov pab pawg sim, nws tau pom ib qho qauv nce thiab poob qis tom qab 60 hnub. Nyob rau 60 hnub ntawm fermentation, cov kev ua enzyme tau mus txog nws qhov siab tshaj plaws, nrog ntau tshaj ntawm 76U / ml. Muaj qhov sib txawv tseem ceeb hauv kev ua si ntawm ib daim duab las ntawm cov pab pawg sim thiab pawg tswj. Lipase cov ntsiab lus ntawm pawg sim siab dua los ntawm 45 hnub rau 75 hnub, thaum tus pab pawg tswj tau qis dua nyob rau lub sijhawm no. Muaj peev xwm maj mam ploj zuj zus. Cov kev ua ntawm kev tiv thaiv sib txawv heev thoob plaws hauv cov txheej txheem fermentation. Qhov kev ua protease siab tshaj plaws hauv pawg sim tau nyob rau hnub 45, nrog kev ua si enzyme ntawm 890u / ml. Cov pab pawg tswj xyuas tau ncav cuag tus nqi siab tshaj plaws ntawm 655U / mL ntawm 15th hnub ntawm fermentation. Lub cellulase kev ua ntawm cov pab pawg sim yog ib txwm siab dua li ntawm cov pab pawg tswj hwm thaum lub sij hawm uas muaj nuj nqis siab tshaj plaws ntawm 30th thiab 60 hnub ntawm fermentation.

Cov tshuaj ntsuab tshiab nrog cov tshuaj tiv thaiv oxidation

Piv nrog cov uas tsis tau ntxiv cov leeg, nyob rau hnub 90 ntawm fermentation, cov kev ua si ntawm daim tawv nqaij txo qis los ntawm 55.71%, thiab kev ua kom cov tub cellulose nce los ntawm 54.19%.
Los ntawm cov kev ua si cuam tshuam nrog cov pab pawg kws sim thiab cov kev tswj hwm, nws tau pom tias muaj cov haujlwm enzyme tsis qhia ib qho ntxiv zuj zus, tab sis nce thiab poob. Yog li ntawd, cov enzymes tsis yog neeg ib txwm hais, ntev dua qhov zoo dua, koj yuav tsum haus lawv nyob rau lub sijhawm.