Beneficial Bioapplications Of Silver Nanoparticles Synthesized By A Marine Crustacean

Thov hu rauoscar.xiao@wecistanche.comyog xav paub ntxiv

Abstract

Silver nanoparticles (AgNPs) muaj kev siv dav. Kev tsim cov AgNPs tuaj yeem tshwm sim los ntawm kev sib txawv ntawm cov tshuaj, lub cev, thiab cov qauv ntsuab. Cov txheej txheem nrov tshaj plaws yog kev siv tshuaj lom neeg. Cov kab mob Marine nthuav tawm ntau yam ntawm bioactivity. Txoj kev tshawb no tau tsim los tsim cov biosynthesis ntawm nyiaj nanoparticles los ntawm marine crustacean extract ntawm cov tawv thiab mos ntawm txiv neej thiab poj niam E.massavensis. Lub microstructure, morphology, thiab optical absorption zog ntawm nanoparticles yog tus cwj pwm los ntawm X-ray diffraction (XRD), scanning electron microscopy (SEM), thiab [IV-pom spectrosconvQhov tsim ntawm cov nyiaj nanoparticles tau lees paub los ntawm-Vis absorption, thiab cov spectrum. tau pom plasmon bands ntawm 441.79-462.74 nm. XRD cov txiaj ntsig tau pom tias cov nanoparticles yog crystalline nyob rau hauv cov xwm, thiab SEM cov duab kuaj pom lub quasi-spherical AgNP's morphological duab. Silver nanoparticles los ntawm marine crustacean extract ntawm qhov nyuaj ntawm txiv neej E.massavensis (HM4) qhia tau qhov zoo tshaj plaws hauv morphology thiab particle loj. Kev soj ntsuam ntawm cytotoxicity ntawm AgNPs (HM4) ntawm cov kab mob qog noj ntshav sib txawv 'cov tshuaj tiv thaiv kab mob, tshuaj tiv thaiv kab mob, tshuaj tiv thaiv kab mob ntshav qab zib, tshuaj tiv thaiv kab mob, tiv thaiv kev laus, thiab tshuaj tiv thaiv kab mob. AgNPs tus cwj pwm tuaj yeem qhia cov ntawv cog lus hauv kev kho mob.

Ntsiab lus:Nyiaj nanoparticles; UV-Vis; SEM; XRD; Biosynthesis; Marine crustacean; Cytotoxicity; Bioapplications.

Thov nias ntawm no kom paub ntxiv

1. Taw qhia

Nanotechnology yog ib ceg loj hlob sai ntawm kev tshawb fawb uas cuam tshuam nrog kev sib txuas thiab kev loj hlob ntawm ntau yam nanomaterials. Lub teb ntawm nanotechnology yog thaj chaw nquag tshaj plaws ntawm kev tshawb fawb hauv cov ntaub ntawv niaj hnub science. Txawm hais tias muaj ntau yam tshuaj nrog rau cov txheej txheem ntawm lub cev, ntsuab synthesis ntawm nanomaterial yog txoj kev tshwm sim tshaj plaws ntawm synthesis [1-4]. Tam sim no, ntau yam hlau nanomaterials tau npaj los ntawm tooj liab, zinc, titanium, magnesium, kub, alginate, thiab nyiaj [5]. Silver nanoparticles AgNPs tau dhau los ua lub ntsiab lus tseem ceeb ntawm kev tshawb fawb hnyav vim tias lawv xaiv ntau yam kev siv hauv thaj chaw xws li catalyst, optics, antimicrobials, thiab biomaterial ntau lawm [6-8]. AgNPs muaj qhov ua haujlwm siab vim qhov loj ntawm qhov loj-rau-ntim piv thiab ua lub luag haujlwm tseem ceeb hauv inhibiting kab mob loj hlob hauv aqueous thiab khoom xov xwm. Piv txwv li, AgNPs tau tshaj tawm tias muaj tshuaj tua kab mob, tshuaj tua kab mob, tshuaj tua kab mob, thiab tshuaj tua kab mob [9].

Cov kab mob Marine yog ib qho chaw nplua nuj ntawm bioactive tebchaw nrog kev cuam tshuam zoo kawg nkaus hauv kev tsim tshuaj, kev lag luam thiab cov khoom siv biotechnological. Nyob rau hauv xyoo tas los no, cov kws tshawb fawb tsom mus rau kev tshawb fawb txog kev sib txuas ntawm nanoparticles los ntawm cov chaw hauv dej hiav txwv [10]. Crustaceans, pawg loj taxonomic nyob rau hauv marine ecosystems, nyob ib tug loj infaunal chaw nyob thiab ua si ib tug tseem ceeb luag hauj lwm nyob rau hauv bioturbation, thiab hloov cov organic cov ntaub ntawv thiab cov as-ham. Crustaceans muaj nuj nqis los ntawm kev lag luam aquaculture raws li qhov zoo tshaj plaws ntawm polyunsaturated fatty acids (PUFAs), thiab lawv muaj peev xwm ntxiv cov roj ntses raws li qhov tseem ceeb lipid Cheebtsam ntawm txau [11]. Lub mantis cw (Erugosquilla massavensis) yog cov crustacean ntau hauv Egypt. Nws yog ib qho ntawm cov tsiaj nyeg tseem ceeb tshaj plaws nyob rau hauv ntau qhov chaw ntiav, chaw sov, thiab subtropical marine chaw nyob. Qhov no mantis cw pom nyob rau hauv qhov siab ntom ntom hauv cov cheeb tsam uas tsim nyog burrowing substrates ntawm nplua xuab zeb thiab xuab zeb av nkos, tshwj xeeb tshaj yog qhov cuam tshuam ntawm dej ntws tawm yog qhov tseem ceeb [12]. E. massavensis stomatopods yog benthic, marine, predatory crustaceans uas nyob rau hauv tiv thaiv burrows.

AgNPs muaj ntau daim ntawv thov kev kho mob ib qho tseem ceeb tshaj plaws yog cov tshuaj tiv thaiv qog nqaij hlav qog nqaij hlav cancer (CRC) uas yog qhov thib ob ua rau mob qog noj ntshav hauv ntau lub teb chaws industrialized [13]. Mob qog noj ntshav hauv plab (CRC) suav txog 700,000 cov neeg tuag thiab 1.4 lab tus neeg mob tshiab tau kuaj pom thoob ntiaj teb ib xyoos twg, ua rau nws yog tus thib ib ua rau cov neeg mob qog noj ntshav tsis haus luam yeeb. Cov qog nqaij hlav uas pib hauv cov hlwb uas nyob rau sab hauv ntawm txoj hnyuv thiab qhov quav yog hu ua mob qog nqaij hlav hauv plab. Feem ntau CRCs tshwm sim nyob rau hauv lub epithelium, ib tug txheej txheem tsav los ntawm noob caj noob ces thiab / los yog epigenetic alterations uas tshwm sim nyob rau hauv tsim ntawm premalignant lesions hu ua adenomas. Cov kab mob qog nqaij hlav hauv plab (CRC) tau tshwm sim los ntawm kev nce zuj zus ntawm cov caj ces thiab kev hloov pauv ntawm cov kab mob epigenetic uas ua rau kev hloov pauv ntawm cov kab mob colonic epithelium mus rau txoj hnyuv adenocarcinoma [14].

Cistanche tuaj yeem tiv thaiv kev laus

Txoj kev tshawb no tau tsim los tsim cov biosynthesis ntawm cov nyiaj nanoparticles los ntawm marine crustacean extract ntawm cov tawv thiab mos ntawm txiv neej thiab poj niam E. massavensis thiab characterize silver nanoparticles uas tsim. Lub cytotoxicity ntawm AgNPs uas tsim los ntawm ib qho nyuaj ntawm txiv neej E. massavensis tau soj ntsuam ntawm cov kab mob qog noj ntshav sib txawv. Antiviral, anti-microbial, anti-diabetic, anti-arthritic, anti-aging, thiab anti-inflammatory zog tau soj ntsuam.

Cov ntaub ntawv thiab cov txheej txheem Sample Collection

Mantis cws (E. massiveness) cov qauv tau txais los ntawm Mediterranean Hiav Txwv ntawm Alexandria los ntawm Eastern Harbor. Cov qauv tau sau thaum hmo ntuj los ntawm (Lub Xya Hli mus txog Lub Kaum Hli) thaum lub caij ntuj sov xyoo 2017 siv cov trawlers coj mus muag. Cov neeg laus uas sau tau E. loj heev tau raug coj mus kuaj hauv cov dej hiav txwv zoo aerated kom paub tseeb tias lawv tseem muaj sia nyob.cov txiaj ntsig cistancheTxiv neej (M) thiab poj niam (F) mantis cw tau yooj yim sib cais raws li thaj chaw ntawm qhov chaw mos thiab qhov muaj lossis tsis muaj chaw mos. Kev soj ntsuam morphometric ntawm txiv neej thiab poj niam E. loj heev tau txiav txim siab los ntawm kev ntsuas lub cev ntev thiab lub cev hnyav. Lawv qhov hnyav yog 17.80 ± 3.79 g thiab 16.90 ± 4.04 g, thiab qhov ntev yog 11.81 ± 1.51 thiab 11.78 ± 1.28 cm rau cov txiv neej thiab poj niam, feem. Kev sib cais ntawm cov leeg ntawm exoskeleton los ntawm Tshem tawm tag nrho cov appendages thiab lub cev tshiab kom deb ntawm carapace thiab khaws cia ntawm -20 degree C thaum xav tau.

Kev npaj ntawm extract

Cov nqaij (mos ib feem; S) thiab plhaub (qhov nyuaj; H) (~ 10 g) tau finely pulverized siv mortar thiab pestle. Cov extract tau ua txog li 100 mL siv ob-distilled Milli-Q dej. Tom qab ntawd cov extract tau lim los ntawm Whatman naj npawb 1 lim ntawv kom cais cov ntaub so ntswg rubbles thiab tau txais cov ntshiab extract.

Synthesis ntawm nyiaj nanoparticles

Cov lim dej tau siv los ua tus neeg sawv cev txo qis thiab stabilizer rau cov synthesis ntawm AgNPs.10 mL ntawm cov filtrate yog tov nrog 90 mL ntawm 1 mM nyiaj nitrate tov nyob rau hauv ib tug 250 mL Erlenmeyer hwj thiab agitated ntawm 60 degree C nyob rau hauv maub. Ib lub raj mis uas muaj 10 mL Milli-Q thiab 90 mL silver nitrate tov tau raug coj los tswj. Qhov kev hloov ntawm cov xim yog visually soj ntsuam kom txog rau thaum cov tsos ntawm ib txwm tsaus xim av xim. Tus cwj pwm ntawm cov khoom siv nyiaj nanoparticles (AgNPs) Cov khoom sib xyaw ua ke (SF1, HF2, SM3, thiab HM4) yog tus cwj pwm los ntawm kev nqus spectroscopy, SEM, thiab XRD.

UV-Vis Spectroscopy

UV-pom spectroscopic tsom xam tau ua tiav ntawm Shimadzu UV 1700. Tom qab 24 teev thiab 4 hnub, lub qhov muag kho qhov muag ntawm cov nanoparticized cov dej nyob hauv cov wavelengths nyob rau hauv lub wavelengths ntawm 300 txog 800 nm thiab plotted qhov tseem ceeb ntawm ib daim duab. X-Ray Diffraction Pattern XRD ntsuas tau kaw rau ntawm (Shimadzu LabX XRD-6100 X-ray diffractometer, Nyiv). Uas tau ua haujlwm ntawm qhov hluav taws xob ntawm 40 kV thiab tam sim no ntawm 30 mA nrog rau qhov excitation ntawm CuK hluav taws xob (?=1.541 Å), nyob rau hauv thaj tsam ntawm lub kaum sab xis ntawm 30 txog 80 degree ntawm qhov ntsuas ntawm 5. feem pua ​​/ min nrog cov kauj ruam dav 0.02 degree Rau XRD ntsuas, cov nyiaj nanoparticles (AgNPs) tau muab tso rau ntawm pre-sashed iav substrates thiab qhuav nyob rau hauv ib lub qhov cub ntawm 60 degree C. Scanning Electron Microscopy Lub morphology ntawm deposited AgNPs ntawm iav substrates tau soj ntsuam. siv scanning electron microscopy (JEOL SEM, JSM-636OLA, Nyiv) ntawm qhov nrawm nrawm ntawm 20 kV. Cov qauv saum npoo yog nqus coated nrog kub rau SEM.

Kev ntsuam xyuas cytotoxicity

Ntau hom kab ntawm tes xws li MCF-7( tib neeg kab mob qog noj ntshav ntawm lub mis), Hepa-2 (tib neeg Hepatocellular carcinoma), thiab CACO (Colorectal carcinoma) tau txais los ntawm VACSERA Tissue Culture Unit. Qhov kev sib raug zoo ntawm cov hlwb tseem muaj sia nyob thiab cov tshuaj concentration tau txuas ntxiv rau 24 teev thiab cov txiaj ntsig tau txais txiaj ntsig tau txiav txim siab los ntawm txoj kev colorimetric [15]. Qhov 50 feem pua ​​​​ inhibitory concentration (IC50) tau kwv yees los ntawm cov duab kos duab ntawm cov koob tshuaj tiv thaiv kab mob rau txhua qhov concentration. Antimicrobial activating Assay Txiav plug txoj kev rau kev tshuaj xyuas cov tshuaj tiv thaiv kab mob rau kev sim cov complexes: sau los ntawm Pridham li al [16] tau ua hauj lwm los txiav txim cov tshuaj tua kab mob ntawm cov khoom xaiv. Qhov nruab nrab txoj kab uas hla ntawm inhibition zones tau sau nyob rau hauv millimeters thiab muab piv rau txhua daim hlau. Cov kab mob tshuaj tua kab mob tau sim tawm tsam Gram-positive kab mob (Staphylococcus aureus, Bacillus subtilis, Streptococcus mutant, Enterococcus faecalis, thiab Streptococcus pyogenes), thiab cov kab mob Gram-negative (Escherichia coli, Salmonella Typhigimurium), thiab plaub hom kab mob. Aspergillus fumigatus, Cryptococcus nanoforms, Candida albicans, thiab Aspergillus Brasilienses) siv txoj kev hloov kho kom zoo. Anti-viral effect Kev ntsuam xyuas ntawm cov tshuaj tiv thaiv kab mob siv Cytopathic Effect Inhibition Assay ntawm ob hom kab mob HAV-10 (tus kab mob siab A) thiab HSV-1(Herpes simplex type 1 virus), qhov kev ntsuam xyuas no raug xaiv los qhia tshwj xeeb inhibition ntawm ib tug biologic muaj nuj nqi, piv txwv li, cytopathic nyhuv (CPE) nyob rau hauv lub susceptible mammalian hlwb[17.

Kev tiv thaiv kev laus

Ua ntej kev tshuaj ntsuam xyuas hauv txhua qhov kev ntsuam xyuas, spectra rau tag nrho cov kev rho tawm tau kaw rau ntawm Cary 300 UV-pom spectrophotometer los xyuas kev cuam tshuam thiab hloov pauv hauv lambda max. Qhov kev ntsuam xyuas ua haujlwm tau ua raws li txoj hauv kev spectrophotometric los ntawm Collagenase assay [18] nrog qee qhov kev hloov kho rau siv hauv microplate nyeem ntawv.txo cov roj cholesterolAnti-inflammatory thiab Anti-arthritic kev ua ub no Anti-inflammatory zog ntawm ob qho tib si crude extract thiab synthesized silver nanoparticles raug soj ntsuam siv ib tug albumin denaturation xeem nrog ib co kev hloov kho [19]. Thaum lub sij hawm, Anti-arthritic kev ua ub no tau soj ntsuam siv U937 tib neeg monocytes (ATCC, Manassas, VA, USA) los kawm txog cov txiaj ntsig ntawm cov qauv ntawm cov tshuaj histamine tso tawm [20].

Kev ntsuam xyuas ntawm Anti-diabetic Potential

Antidiabetic kev ua ub no rau ob qho tib si crude extract thiab synthesized silver nanoparticles raug soj ntsuam los ntawm ob txoj kev sib txawv. Thawj yog ib qho -glucosidase inhibitory kev ua haujlwm uas tau ntsuas raws li txoj kev tau piav qhia los ntawm Koj li al. [21]. Qhov thib ob yog, a-amylase inhibitory kev ua haujlwm uas tau txiav txim siab los ntawm kev ntsuam xyuas colorimetric microplate siv cov txheej txheem tsim tau zoo [22].

Kev txheeb cais

Cov ntaub ntawv tau nthuav tawm raws li qhov tseem ceeb ± SD (tus qauv sib txawv) thiab kev txheeb xyuas kev txheeb xyuas tau ua los ntawm ib txoj kev tsom xam ntawm qhov sib txawv (ANOVA) los ntsuas qhov sib txawv tseem ceeb ntawm pab pawg kho mob. Cov txheej txheem rau kev txheeb xyuas qhov tseem ceeb tau teeb tsa ntawm p Tsawg dua lossis sib npaug rau 0.05. Txhua qhov kev txheeb xyuas kev txheeb xyuas tau ua tiav siv SPSS statistical version 17 software pob (SPSSQ Inc., USA). Cov txiaj ntsig thiab Kev Sib Tham Nws tau ua tiav qhov kev sib txuas ntawm cov nyiaj nanoparticles los ntawm kev txo cov tshuaj lom neeg. Kev tsim ntawm cov nyiaj nanoparticles tau pom pom pom nrog cov xim daj (xim av) tom qab incubation. Cov xim xim av tsim ntawm cov qauv qhia tau hais tias cov colloidal nanoparticles tsim yog cov txheej txheem synthesis yog dominated los ntawm cov nplej ntawm cov nyiaj nanoparticles.

UV pom spectroscopy

Ultraviolet thiab pom spectrometry yog yuav luag siv rau kev soj ntsuam kom muaj nuj nqis ntawm cov tebchaw paub tias muaj nyob rau hauv cov qauv. UV-pom spectroscopy yog ib qho ntawm cov kev siv dav tshaj plaws rau cov qauv kev ua haujlwm ntawm cov nyiaj nanoparticles. Nyob rau hauv cov hlau nanoparticles xws li nyob rau hauv cov nyiaj, cov conduction band thiab valence band nyob ze heev rau ib leeg nyob rau hauv uas electrons txav dawb do. Cov hluav taws xob dawb no ua rau lub ntsej muag plasmon resonance (SPR) nqus band [23-26], tshwm sim vim kev sib koom ua ke ntawm cov hluav taws xob ntawm cov nyiaj nanoparticles hauv resonance nrog lub teeb nthwv dej [27].cistanche deserticola phivOptical absorption spectra of silver nanoparticles are dominated by SPR which shows a shift of the red end or blue end nyob ntawm qhov loj me, cov duab, thiab lub xeev ntawm kev sib sau ua ke ntawm cov nyiaj nanoparticles [28]. Kev nqus spectrum ntawm cov qauv (SF1, HF2, SM3, thiab HM4) qhia tau zoo plasmon bands ntawm 441.79-462.74 nm tom qab 24 teev, uas yog cov yam ntxwv ntawm nanosized nyiaj. UV-Vis absorption spectra ntawm AgNPs cov qauv (SF1, HF2, SM3, thiab HM4) tau tshwm sim hauv daim duab 1.

Cov qauv nyiaj nanoparticles (SF1 thiab HM2) tau pom qhov tshwm sim hauv cov khoom siv hluav taws xob nqus ntawm cov qhab nia nyob ntawm 447.16 nm thiab 441.79 nm tom qab 24 teev (1 hnub), feem cuam tshuam nrog qee qhov tsis xwm yeem. Thaum nqus plua plav ntawm SM3 thiab HM4 cov qauv tshwm sim ntawm qhov ntev wavelengths txuam nrog me me roughly spherical thiab spherical nanoparticles.

Cov tshuaj tiv thaiv sib xyaw pom cov plasmon resonance absorption band nrog qhov siab tshaj plaws ntawm 462.74 nm thiab 453.65 nm tom qab 24 teev, raws li qhia tau hais tias muaj lub ntsej muag kheej kheej lossis roughly kheej kheej cov nyiaj nanoparticles. Kev nthuav dav ntawm lub ncov qhia tias cov khoom yog polydispersed [29,30].

Kev ruaj ntseg ntawm cov khoom siv nyiaj nanoparticle daws tau raug soj ntsuam los ntawm kev sau cov UV-vis spectra ntawm lub sijhawm 1 thiab 4 hnub. Tsis muaj qhov pom tseeb hloov pauv hauv qhov chaw siab tshaj ntawm cov nyiaj nanoparticles (SF1, SM3, thiab HM4), tshwj tsis yog qhov nce hauv absorbance. Kev nqus tau nce ntxiv qhia tias cov nyiaj nanoparticles nce ntxiv. Qhov chaw ruaj khov ntawm lub ncov absorbance qhia tias cov khoom tshiab tsis sib sau ua ke. Raws li rau cov qauv HF2, txoj hauj lwm ntawm lub ncov muaj me ntsis liab hloov (451.06 nm), implying qhov pib ntawm nanoparticle aggregation.cistanche noj redditSEM tsom xam Nyiaj nanoparticles tau raug SEM micrograph tsom xam kom nkag siab txog topology ntawm nyiaj ions. Cov nyiaj nanoparticle morphology tau kawm los ntawm kev tshawb xyuas electron microscopy (SEM). SEM micrographs ntawm SF1, HF2, SM3, thiab HM4 nanoparticles raug muab tso rau hauv daim duab 2.

Raws li SEM tsom xam, cov nyiaj nanoparticles yog spherical (nyob rau hauv cov ntaub ntawv ntawm HM4), roughly spherical (nyob rau hauv cov ntaub ntawv ntawm SM3), phaj, thiab ib co tsis xwm yeem (nyob rau hauv cov ntaub ntawv ntawm SF1 thiab HF2). XRD Analysis Cov qauv ntawm cov nyiaj nanoparticles npaj tau raug tshawb xyuas los ntawm X-ray diffraction (XRD) tsom xam. XRD ntawm SF1, HF2, SM3, thiab HM4 nanoparticles yog tso tawm nyob rau hauv daim duab 3.

Qhov twg '入' yog lub wavelength ntawm X-Ray (0.1541 nm),' ' yog FWHM (tag nrho dav ntawm ib nrab siab tshaj), 'θ' yog lub diffraction lub kaum sab xis, thiab 'D's particle diameter (loj) . X-ray diffraction qauv ntawm cov synthesized nanoparticles (SF1) qhia diffraction peaks ntawm 20=32.319,32.779,46.70 degree thiab 61.349, uas yog feem ntau indexed rau (111), (111), (210) thiab (310) lattice dav hlau. X-ray diffraction qauv ntawm cov synthesized nanoparticles (HF2) qhia diffraction peaks ntawm 20=32.10 degree 39.28 degree thiab 61.24 degree, uas tuaj yeem ua raws indexed rau (111), (200), thiab (310) lattice dav hlau. X-ray diffraction qauv ntawm cov synthesized nanoparticles (SM3) qhia diffraction peaks ntawm 20=32.72 degree, 48.68 degree thiab 61.20 degree, uas yog feem ntau indexed rau (111), (211), thiab (310) lattice dav hlau. X-ray diffraction qauv ntawm cov synthesized nanoparticles (HM4) qhia diffraction peaks ntawm 20=32.62 degree, 48.58 degree thiab 59.46 degree, uas yog feem ntau indexed rau (111), (211), thiab (300) lattice dav hlau. Qhov siab-siv peaks rau cov nyiaj nanoparticles hauv cov qauv (SF1, HF2, thiab SM3) tau pom ntawm 20=61.34 degree ,61.24 degree , thiab 61.20 degree , feem cuam tshuam rau (310) kev xav. Qhov no tau lees paub cov qauv lattice los ua bcc (lub cev-centered cubic).

Ib tug xov tooj ntawm Bragg reflections nyob rau hauv lub (111), (21l), thiab (300) txheej ntawm lattice dav hlau raug soj ntsuam rau cov nyiaj nanoparticles qauv (HM4). Qhov siab siv rau cov ntaub ntawv fcc feem ntau yog (11l) kev xav, uas tau pom hauv cov qauv los ntawm qhov siab tshaj plaws ntawm 20=32.62 degree . Qhov no tau lees paub cov qauv lattice ua fcc (lub ntsej muag-centered cubic). Cov qauv nyiaj nanoparticles (SF1, HF2) thiab (SM3, HM4) cov ntaub ntawv tau nthuav tawm hauv Table 1 (a, b), feem. Nws tau pom tias kev sib koom ua ke ntawm bcc (SFl, HF2, thiab SM3) thiab fcc (HM4) cov qauv siv lead ua tshwm sim nrog kev hloov pauv ntawm cov neeg ua haujlwm txo qis (qhov muag thiab tawv ntawm lub cev). Lattice tas li tau kwv yees siv cov qauv, a =d*√(h2 plus k2 plus 12) rau cov nyiaj nanoparticles

cov qauv (SF1, HF2, SM3, thiab HM4). Qhov nruab nrab ntawm plaub qhov tseem ceeb suav los ntawm qhov tseem ceeb ntawm qhov tau los ntawm cov ntaub ntawv rau cov peaks pom yog 4.66,4.73,4.69 thiab 4.66 A, feem. Nws tau pom tias cov lattice tsis ntawm cov nyiaj nanoparticles txo qis nrog kev txo qis ntawm qhov loj me. Qhov nruab nrab loj ntawm nanoparticle particle qauv (SF1, HF2, SM3, thiab HM4) tau pom tias yog 67.07, 557.03,80.66, thiab 20.63 nm, raws li. Nyob rau hauv cov ntaub ntawv ntawm particles synthesized nyob rau hauv HM4 xov xwm qhov nruab nrab particle loj yog 20.63 nm thaum lub particles synthesized nyob rau hauv SF1, HF2 thiab SM3 yog loj nyob rau hauv nruab nrab.cistanche extract cov txiaj ntsigCov txiaj ntsig XRD qhia tau hais tias cov nanoparticles yog crystalline nyob rau hauv cov xwm thiab cov muaju yog cubical nyob rau hauv cov duab. HF2 tau pom tias muaj qhov loj me me. Cov nyiaj loj dua tau ua ke tuaj yeem yog vim kev sib sau ntawm cov me me. Kev soj ntsuam ntawm XRD cov qauv tau lees paub cov txiaj ntsig tau los ntawm UV-Vis spectra thiab electron micrographs ntawm cov khoom sib txuas nanoparticles.

Bioapplications

Raws li kev pom zoo ntawm biosynthesis ntawm silver nanoparticles los ntawm marine crustacean extract ntawm cov tawv thiab mos ntawm txiv neej thiab poj niam E. loj heev (SF1, HF2, SM3, thiab HM4), exploiting qhov zoo tshaj plaws tshwm sim ntawm AgNPs (HM4) rau kev ntsuam xyuas ntawm cytotoxicity ntawm cov kab mob qog noj ntshav sib txawv antiviral, anti-microbial, anti-diabetic, anti-arthritic, anti-aging thiab anti-inflammatory zog.

Cov txiaj ntsig tau los ntawm kev ntsuas cytotoxicity tawm tsam cov kab sib txawv rau ob qho tib si crude extract thiab AgNPs ntawm ib qho nyuaj ntawm txiv neej E. massavensis (Table 2) qhia tias, AgNPs uas ua los ntawm ib qho nyuaj ntawm txiv neej E. massavensis muaj zog cytotoxic zog tiv thaiv txhua tus. cov kab mob uas kuaj tau (muab los ntawm txoj hnyuv, lub mis, thiab lub siab mob cancer) dua li cov kua txiv hmab txiv ntoo los ntawm cov txiv neej tawv E. massavensis. IC50 qhov tseem ceeb ntawm cytotoxicity tau txais los ntawm AgNPs yog ze li ntawm cov uas tau txais los ntawm cov tshuaj siv, tshwj xeeb tshaj yog mob qog noj ntshav. Cov txiaj ntsig no tau ua raws li cov kev tshawb fawb yav dhau los sib txawv uas tau ua pov thawj tias AgNPs tau tsim los ntawm zib ntab muv extract tau pom tias muaj kev sib txheeb siab tiv thaiv CACO cell kab tau los ntawm tib neeg cov kab mob qog noj ntshav nrog 58.6 feem pua ​​​​ inhibition [32,33].Lwm txoj kev tshawb fawb qhia tias AgNPs muaj peev xwm ua tau. txo kev muaj peev xwm ntawm Dalton's lymphoma ascites qog [34]. AgNPs los ntawm cov nroj tsuag tshuaj xws li Taraxacum officinale thiab Commelina nudiflora tau pom tias lawv cov txiaj ntsig cytotoxic siab rau tib neeg lub siab mob hlwb (HepG2) thiab cov qog nqaij hlav hauv plab (HCT-116) ​​[35,36]. Nws tuaj yeem piav qhia los ntawm qhov tseeb tias hauv cov hlwb, cov nanoparticles yooj yim hla lub cell membrane thiab cuam tshuam nrog intracellular macromolecules zoo li cov protein thiab DNA. Biologically synthesized AgNPs muaj peev xwm rau alternation cell morphology ntawm cov qog nqaij hlav cancer uas yog qhov taw qhia ntxov rau apoptosis uas tuaj yeem txiav txim siab los ntawm cov qauv hloov hauv cov hlwb [37]. Cov ntaub ntawv tau txais los ntawm kev ntsuam xyuas tshuaj tua kab mob ntawm ob qho tib si crude thiab AgNPs los ntawm lub plhaub ntawm E. massivansis (Table 3) qhia tau hais tias muaj kev tiv thaiv kab mob zoo dua rau cov kab mob Gram-positive, (Staphylococcus aureus, Streptococcus mutants, Bacillus subtilis, Enterococcus faecalis, thiab Streptococcus faecalis. pyogenes) los ntawm inhibition zones ranged ntawm 9-15 hli txoj kab uas hla. Thaum lub sij hawm, crude extract pom tsis muaj kev ua si. Ntawm qhov tod tes, AgNPs tau pom tias muaj kev tiv thaiv kab mob zoo tiv thaiv kab mob Gram-negative (Salmonella typhimurium, Pseudomonas aeruginosa, Escherichia coli, thiab Klebsiella pneumonia) nrog thaj tsam inhibition ranged ntawm 10-14 hli txoj kab uas hla. Cov kua txiv hmab txiv ntoo los ntawm lub plhaub ntawm txiv neej E.massivansis tau pom cov txiaj ntsig zoo sib xws nrog cov cheeb tsam inhibition xws li 10-16 hli hauv txoj kab uas hla tsuas yog tawm tsam E.coli uas tsis qhia txog kev ua haujlwm. Los ntawm qhov zoo sib xws nrog cov kab mob Gram-positive, AgNPs kuj tau pom qhov nruab nrab ntawm cov tshuaj tiv thaiv kab mob tiv thaiv Aspergillus fiumigatus, Cryptococcus nanoforms, Candida albicans, thiab Aspergillus Brasilienses nrog inhibition zones ntawm 10-15 hli txoj kab uas hla. Txawm li cas los xij, crude extract qhia tsis muaj kev ua haujlwm. Cov txiaj ntsig no yog nyob rau hauv txoj kab nrog rau lwm cov kev tshawb fawb yav dhau los tau tshaj tawm tias AgNPs los ntawm hemolymph ntawm marine crabs (Carcinus maenas, Ocypode quadrata, thiab Polychaeta) tau pom tias muaj kev tiv thaiv kab mob siab tiv thaiv kab mob sib txawv. Nws tuaj yeem sib tham raws li lawv qhov chaw ua haujlwm loj ntawm AgNPs uas ua rau lawv ua tiav kev sib cuag nrog cov kab mob zoo dua. Nanoparticles tau txais adsorb rau hauv cov cell membrane thiab nkag mus rau hauv cov kab mob hauv cov kab mob uas cuam tshuam nrog cov protein uas muaj sulfur hauv cov cell membrane ntawm cov kab mob thiab cov phosphorus txuas ntxiv zoo li DNA. Cov AgNPs ua rau inhibition ntawm replication ntawm DNA ntawm ib tug kab mob cell uas ua rau inhibits cell division uas ua rau cov kab mob cell tuag [38,39]. Lwm daim ntawv thov tseem ceeb ntawm AgNPs yog cov tshuaj tiv thaiv kab mob.

Cov txiaj ntsig tau txais hauv peb txoj kev tshawb fawb tau tshaj tawm tias cov tshuaj tiv thaiv kab mob ntawm AgNPs uas tau tsim los ntawm exoskeleton ntawm txiv neej E. loj heev tau pom tias muaj cov tshuaj tiv thaiv kab mob nruab nrab tiv thaiv HAV-10 thiab tsis muaj zog tiv thaiv HSV-1(Table 4) . Ntawm qhov tod tes, crude extract los ntawm ib qho nyuaj ntawm txiv neej E.massavensis tsis pom muaj kev tiv thaiv kab mob. Cov txiaj ntsig no tau pom zoo nrog kev tshawb fawb yav dhau los uas tau qhia tias qhov cuam tshuam ntawm AgNPs rau ntau hom kab mob kis tau zoo li Human Immunodeficiency Virus Type 1 (HIV)Herpes Simplex Virus Type 1 HSV-1, Hepatitis B Virus (HBV), Monkeypox Virus, Tacaribe virus (TCRV) thiab Respiratory syncytial virus [40]. AgNPs uas tau tsim los ntawm lub plhaub ntawm txiv neej E. loj heev kuj pom tias muaj kev tiv thaiv kev laus ntau dua li cov khoom siv raw. Cov txiaj ntsig no yog nyob rau hauv tib txoj kab nrog ntau cov kev tshawb fawb yav dhau los uas tau qhia txog lub luag haujlwm ntawm AgNPs hauv kev tiv thaiv UVB-induced photoaging thiab lub luag haujlwm ntawm nanoparticles hauv Cosmeceuticals siv rau ntawm daim tawv nqaij, plaub hau, ntsia thawv, thiab daim di ncauj [41,42]. AgNPs synthesized los ntawm cov exoskeleton ntawm txiv neej E. loj heev tau pom tias muaj kev tiv thaiv kev mob caj dab uas siv cov inhibition ntawm protein denaturation txoj kev. Txawm hais tias crude extract muaj tsawg heev anti-Arthritic kev ua piv nrog Diclofenac sodium raws li tus qauv compound (Table 5). Cov txiaj ntsig no tau pom zoo nrog txoj kev tshawb fawb yav dhau los uas tau tshaj tawm tias AgNPs los ntawm cov hlab ntsha hauv cov hlab ntsha tuaj yeem siv los ua cov tshuaj tiv thaiv kab mob uas muaj zog vim muaj cov tshuaj bioactive uas siv los tiv thaiv o nrog nws cov mob txuam thiab txo cov tsos mob ntawm kev txav mus los yog qhov tseem ceeb hauv kev kho mob caj dab. [43, 44]. Nws tau raug tshaj tawm tias ib qho ntawm cov yam ntxwv ntawm ntau cov tshuaj uas tsis yog-steroidal anti-inflammatory yog lawv lub peev xwm kom ruaj khov thiab tiv thaiv denaturation [45].

Nyob rau hauv txoj kev tshawb no, AgNPs (HM4) uas yog synthesized los ntawm ib qho nyuaj ntawm txiv neej E.massavensis muaj ib tug ntau dua anti-diabetic muaj peev xwm ntawm -glucosidase thiab -amylase inhibitory kev ua ub no tshaj crude extract raws li piv nrog Acarbose raws li ib tug qauv compound (Table 5) . Cov txiaj ntsig no tau pom zoo nrog cov kev tshawb fawb yav dhau los sib txawv tau tshaj tawm tias qhov txo qis hauv cov ntshav qab zib hauv cov nas kho nrog AgNPs siv P. sapota thiab Lonicera japonica nplooj extract thiab pom tias AgNPs nthuav tawm cov tshuaj tiv thaiv ntshav qab zib, raws li kev soj ntsuam hauv vitro thiab hauv vivo. SNPs tau elucidated raws li cov tshuaj tiv thaiv kab mob uas ua rau txo cov ntshav qabzib [46-48].

Cov lus xaus

Silver nanoparticles synthesized los ntawm tshuaj txo txoj kev los ntawm kev siv marine crustacean extract ntawm cov tawv thiab mos ntawm txiv neej thiab poj niam E. loj heev. Cov nanoparticles tau tsim los ntawm UV-Vis spectroscopy, SEM, thiab XRD. Kev soj ntsuam ntawm XRD cov qauv tau lees paub cov txiaj ntsig tau los ntawm UV-Vis spectra thiab electron micrographs ntawm cov khoom sib txuas nanoparticles. AgNPs (HM4) tau nthuav tawm cytotoxic kev ua ntawm cov kab mob qog noj ntshav sib txawv los tiv thaiv kab mob, tiv thaiv kab mob, tiv thaiv mob ntshav qab zib, tiv thaiv mob caj dab, tiv thaiv kev laus, tiv thaiv kab mob. AgNPs da thiab 7.1 characterization tej zaum yuav qhia tau cov ntawv cog lus hauv kev kho mob.

Kab lus no yog muab rho tawm los ntawm Egypt. J. Cheem. Vol. 64, nr 8 p. 4653 - 4662 (2021)