Antifatigue Activity Ntawm Phenylethanoid-nplua nuj Extract Los ntawm Cistanche Deserticola
Mar 12, 2022
Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791
A phenylethanoid- nplua nujrho tawm(ECD) ntawmCistanche deserticolaYC Ma, cov nroj tsuag holoparasitic thiab cov tshuaj suav tshuaj muaj txiaj ntsig, tau raug soj ntsuam rauantifatigue kev uahauv ICR nas. ECD ({{0}}.25, 0.50, 1.00 g/kg) tau muab tshuaj rau cov nas rau 3 lub lis piam. Lub sij hawm ua luam dej kom qaug zog tau ntev dua hauv cov pab pawg kho mob (0.50, 1.00 g / kg) dua li hauv pawg tswj hwm (p <0.01). cov="" ntshav="" creatine="" kinase,="" lactate="" dehydrogenase,="" thiab="" lactic="" acid="" qib="" tau="" txo="" qis="" hauv="" cov="" pab="" pawg="" kho="" mob="" piv="" nrog="" pawg="" tswj="" hwm,="" thaum="" cov="" ntsiab="" lus="" ntawm="" hemoglobin="" thiab="" qabzib="" tau="" nce="" ntau.="" hauv="" kev="" xaus,="" ecd="" tau="" tshwm="" sim="" los="" txhim="" kho="" lub="" peev="" xwm="" ua="" luam="" dej="" ntawm="" cov="" nas="" los="" ntawm="" kev="" txo="" cov="" leeg="" nqaij,="" ncua="" kev="" sib="" sau="" ntawm="" lactic="" acid,="" thiab="" txhim="" kho="" lub="" zog="" cia.="" cov="" txiaj="" ntsig="" no="" muab="" cov="" pov="" thawj="" scientific="" rau="" kev="" coj="" noj="" coj="" ua="" hauv="" suav="" teb="" ntawm="" c.="" deserticola.="" copyright="" ©="" 2009="" john="" wiley="" &="" sons,="">
Ntsiab lus:Cistanche deserticola; antifatigue kev ua; phenylethanoid; biochemical tsis muaj.
Antifatigue kev uahauvCistanche deserticola
Taw qhia
Cistanche deserticola(Orobanchaceae), ib tsob nroj holoparasitic, tuaj yeem pom hauv thaj av suab puam ntawm sab hnub poob ntawm Tuam Tshoj. Nws yog siv los ua tshuaj suav tshuaj tonic rau lub raum tsis muaj zog, ua rau lub cev tsis muaj zog, mob hauv lub duav thiab lub hauv caug, poj niam tsis muaj menyuam, thiab cem quav vim lub plab zom mov hauv cov laus. Cov nroj tsuag hu ua 'desert ginseng' los ntawm cov neeg nyob hauv zos vim nws cov nyhuv ginseng zoo li tonic. Qhov tseeb, C. deserticola tau nquag siv hauv cov ntawv sau thaum ub rau kev txhim kho lub cev lub zog. Nws txoj kev ua tau zoo hauv kev ua kom lub cev muaj zog tau sau tseg hauv phau ntawv kho mob Suav thaum ub (Shang, 1993).
Pharmacological kev tshawb fawb pom tau tiasCistanche deserticola rho tawmmuaj antinociceptive, antiinflammatory (Lin li al., 2002), thiab sedative teebmeem (Lu, 1998).Phenylethanoidsthiab polysaccharides, uas yog cov khoom tseem ceeb ntawm cov nroj tsuag no, tau tshaj tawm tias muaj cov tshuaj tiv thaiv oxidative (Xiong li al., 1996), hepatocyte tiv thaiv cov nyhuv (Xiong li al., 1998), TSIS MUAJ radical-scavenging kev ua (Xiong li al., 2000), thiab kev tiv thaiv kab mob (Dong et al., 2007). Txawm li cas los xij, qee qhov kev tshawb fawb tau tsom mus rauantifatigue kev ua. Ib txoj kev tshawb fawb yav dhau los pom tau tias muaj cov khoom siv polysacchariderho tawmpab me ntsis rau covantifatigue kev uantawmCistanche deserticola(cov ntaub ntawv tsis qhia). Nws yog hypothesized tiasphenylethanoid- nplua nujrho tawm(ECD), uas muaj lwm pab pawg loj ntawm cov khoom siv tshuaj, tuaj yeem ua lub luag haujlwm tseem ceeb hauv nwsantifatigue kev ua. Lub hom phiaj ntawm txoj kev tshawb no yog los soj ntsuam cov txiaj ntsig ntawm ECD ntawm lub cev lub zog thiab kev ua siab ntev ntawm cov nas thaum yuam kev ua luam dej.
antifague acitivityhauvCistanche deserticola
Cov khoom siv thiab cov txheej txheem
Cov khoom cog.Cov txiv hmab txiv ntoo tshiab ntawm C. deserticola tau muab los ntawm Yongning C. deserticola cog (Ningxia, Tuam Tshoj). Ib daim ntawv pov thawj tau muab tso rau hauv Herbarium ntawm Lub Tsev Haujlwm Saib Xyuas Kev Noj Qab Haus Huv (Suav Academy ntawm Kev Kho Mob Science, Tuam Tshoj).
Kev npaj ntawm cov extracts.Cov khoom siv huab cua qhuav yog hmoov thiab muab rho tawm los ntawm percolation nrog 70 feem pua EtOH. Lub percolate tau evaporated nyob rau hauv lub siab txo thiab cov kua residual tau lim. Cov filtrate tau concentrated thiab chromatographed ntawm ib qho AB -8 macroporous resin (Cov Tshuaj Cog ntawm Nankai University, Tuam Tshoj) kem siv 50 feem pua thiab 95 feem pua EtOH hauv dej li eluants. Lub 50 feem pua EtOH eluant tau concentrated thiab qhuav nyob rau hauv kev txo qis kom tau txais aphenylethanoid- nplua nuj feem. Cov txiaj ntsig ntawm feem yog kwv yees li 3.33 feem pua thiab cov ntsiab lus ntawm covphenylethanoidsyog 58.9 feem pua. Verbascoside thiab echinacoside yog ob lub ntsiab lus tseem ceeb hauv ntu no, nrog rau lawv cov ntsiab lus yog 5.6 feem pua thiab 33.3 feem pua.
Tsiaj txhu, pab pawg, thiab kev sim tsim.Plaub lub lis piam-laus txiv neej ICR nas tau muab faib ua plaub pawg. ECD ntawm koob tshuaj 0.25, 0.50, 1. , thaum pawg tswj hwm tau txais tib lub ntim ntawm 0.9 feem pua saline. Cov nas tau raug cob qhia ua luam dej rau 20 feeb ob zaug hauv ib lub lis piam los ua luam dej. Tom qab 3 lub lis piam, cov nas yoo mov ib hmos ua ntej yuam kev ua luam dej lossis kuaj ntshav. Txhua qhov kev sim tau ua tiav raws li National Institutes of Health Guide for Care and Use of Laboratory Tsiaj thiab tau txais kev pom zoo los ntawm Tsiaj Ethics Committee ntawm Lub Tsev Haujlwm Saib Xyuas Kev Kho Mob, Suav Academy ntawm Kev Kho Mob Sciences.
Qhov hnyav-loaded ua luam dej xeem. Tsib caug-rau nas tau siv nyob rau hauv qhov kev sim no raws li qhia hauv Table 1. Cov txheej txheem kuaj tau zoo ib yam li tau piav qhia los ntawm Porsolt li al. (1977). Luv luv, 30 min tom qab kev tswj hwm intragastric kawg, cov nas tau muab tso rau hauv ib lub pas dej da dej
(65 × 45 × 40 cm) nrog 35 cm qhov tob ntawm dej tuav ntawm 25.5 ± 0.5 ºC. Ib lub tin hlau (5 feem pua ntawm lub cev qhov hnyav) tau thauj khoom ntawm tus Tsov tus tw ntawm tus nas. Kev qaug zog tau txiav txim siab los ntawm kev saib tsis ua luam dej thiab lub sijhawm ua luam dej tau suav tias yog lub sijhawm siv los ntawm tus nas ntab hauv dej nrog kev tawm tsam thiab ua kom tsim nyog txav mus txog thaum nws qaug zog thiab poob dej. Cov nas tau raug ntsuas kom qaug zog thaum lawv tsis tuaj yeem nce mus rau saum npoo dej kom ua pa tsis pub dhau 10 s. Lub sijhawm ua luam dej kom qaug zog tau siv los ua qhov ntsuas ntawm qhov yuam kev ua luam dej.
Kev txiav txim siab ntawm cov ntaub so ntswg glycogen.Tom qab ntsuas qhov hnyav-loaded ua luam dej, daim siab thiab gastrocnemius cov leeg ntawm nas tau raug muab tshem tawm tam sim ntawd, khov rau hauv cov dej khov qhuav, thiab khaws cia ntawm -80 ºC kom txog thaum kev soj ntsuam cov ntsiab lus glycogen tau ua. Cov ntsiab lus glycogen raug ntsuas raws li txoj kev piav qhia lwm qhov (Chun thiab Yin, 1998).
Kev txiav txim siab ntawm cov ntshav biochemical tsis. Nrog lub hom phiaj ntawm kev sau cov ntshav txaus rau kev ntsuam xyuas, lwm 48 nas tau siv. Tom qab ntawd 30 feeb tom qab qhov kev tswj hwm intragastric kawg ntawm ECD, cov nas raug yuam kom ua luam dej hauv lub pas dej da dej (qhov hnyav-tsis hnyav) rau 90 min. Tom qab tshuaj loog nrog ether, cov ntshav tau sib sau ua ke hauv cov hlab ntsha heparinized thiab tsis muaj cov hlab ntshav los ntawm extirpating lub qhov muag sab laug. Tag nrho cov ntshav kuaj tau siv los txiav txim siab hemoglobin thiab lactic acid raws li HiCN (hemoglobin cyanide) txoj kev (ASTM F 756-93, -86) thiab hloov kho Barker-Summerson txoj kev (Pryce, 1969), feem. . Cov ntshav tau npaj los ntawm centrifugation ntawm 1000 × g, 4 ºC rau 15 min thiab cov qib ntawm cov ntshav creatine kinase, lactate dehydrogenase, urea nitrogen, tag nrho cov protein, thiab qabzib tau txiav txim siab los ntawm kev ntsuas biochemical tsis siv neeg (Hitachi 7060, Hitachi, Ltd, Nyiv. ) nrog cov khoom siv coj mus muag (Biosino Biotechnology Co., Ltd, Beijing, Suav).
Kev txheeb cais.Cov txiaj ntsig tau qhia raws li qhov nruab nrab ± SD. Kev sib piv ntawm pab pawg tau tsim los ntawm Tub Ntxhais Kawm T-test.
antifatigue kev uahauvcistanche deserticola
TSEEM CEEB THIAB PAUB
Table 1 qhia tau hais tias lub sij hawm ua luam dej tau ntev dua nyob rau hauv pawg tswj hwm ECD (0.50, 1.00 g/kg). Qhov kev tshwm sim no tau pom tias ECD muaj ib qhotshuaj tiv thaivnyhuv. Txhawm rau kom paub meej nws cov txheej txheem, cov biochemical tsis muaj feem cuam tshuam txog kev qaug zog tau raug soj ntsuam hauv ob qho tib si ECD-kho thiab tsis kho cov nas ua luam dej.
Creatine kinase thiab lactate dehydrogenase paub tias yog qhov qhia tseeb ntawm cov leeg nqaij puas tsuaj (Burr li al., 1997; Coombes thiab McNaughton, 2000). Kev nce qib ntawm creatine kinase thiab lactate dehydrogenase hauv cov ntshav qhia tau hais tias cov leeg mob tau tshwm sim, lossis tshwm sim. Table 2 qhia tau hais tias lactate dehydrogenase thiab creatine kinase qib tau txo qis hauv cov pab pawg neeg tswj hwm ECD (0.50 g / kg lossis 1.00 g / kg), qhia tias ECD tuaj yeem txo cov leeg mob thaum yuam ua luam dej.
Hemoglobin feem ntau tau hais tias yog ib qho ntawm cov tseem ceeb uas tuaj yeem txhim kho kev muaj peev xwm ua tau zoo los ntawm kev nqa oxygen mus rau cov ntaub so ntswg. Cov theem ntawm lactic acid, raws li ib tug metabolic by-product ntawm anaerobic glycolysis, muaj ib tug inverse kev sib raug zoo rau lub sij hawm ua luam dej. Nws tau pom tias qib hemoglobin tau nce siab thiab lactic acid tau txo qis hauv cov pab pawg ECD uas tswj hwm (0.50, 1.00} g / kg).
Strikingly, qhov sib txawv me me tau pom nyob rau hauv cov ntsiab lus glycogen ntawm lub siab lossis cov leeg ntawm cov tswj thiab ECD-kho pab pawg tom qab kuaj ua luam dej (Table 2). Raws li paub, glycogenolysis predominates tshaj gluconeogenesis thaum lub zog ua haujlwm hnyav. Yog li, qhov nce me ntsis ntawm lub siab glycogen, nrog rau kev nce ntxiv ntawm cov piam thaj hauv cov pab pawg kho ECD (0.50, 1.00} g / kg), qhia tias ECD tuaj yeem txhim kho qhov khaws cia ntawm lub siab glycogen.
Hauv kev xaus, ECD tau txhim kho lub peev xwm ua luam dej ntawm cov nas los ntawm kev txo cov leeg nqaij, ncua kev sib sau ntawm lactic acid, thiab txhim kho lub zog cia. Txawm li cas los xij, kev tshawb fawb ntxiv tseem xav tau los piav qhia qhov tseeb ntawm cov txiaj ntsig ntawm ECD ntawm kev qaug zog thiab / lossis kev ua haujlwm ntev.
Kev lees paub
Txoj haujlwm no tau txhawb nqa los ntawm National Natural Science Foundation ntawmTuam Tshoj (No. 30672659) thiab Ningxia Science thiab Technology Office(No. 05GG-10807). Cov kws sau ntawv ua tsaug rau Dr. Elaine Waldron hauvJohns Hopkins University School of Medicine rau nws txoj kev pab hauvtshawb xyuas daim ntawv no.
antifatigue kev uahauvcistanche deserticola
Los ntawm: 'Antifatigue Activity ntawm Phenylethanoid-nplua nuj Extract los ntawm Cistanche deserticola' los ntawmRun-Lan Cai et.al.
----PHYTOTHERAPY Kev Tshawb Fawb Phytother. Res. 24: 313–315 (2010) Tshaj tawm hauv online 16 Lub Xya Hli 2009 hauv Wiley InterScience (www.interscience.wiley.com) DOI: 10.1002/ptr.2927
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